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Since the introduction of FAST qPCR, the world of real-time PCR/qPCR has entered a revolutionary era. By using special instruments and optimized master mixes aimed at speed and efficiency, this technology allows you to accelerate your qPCR applications without compromising on specificity and sensitivity.
Real-time PCR, also known as qPCR, is undoubtedly an impressive method for measuring the amplification of target DNA in real time. However, some people may despair at the abundance of terms, protocols and kits on the market. This begs the question: are reliable qPCR results only achievable through magic or is it ultimately more about the art of precise fine-tuning?
We can reassure you: in fact, with just a few tricks you can optimize your qPCR so that you can easily achieve reliable and accurate results. Whether you are already an expert in the field of qPCR or just taking your first steps into this fascinating world, this article will guide you through the key factors that will ensure your qPCR assays produce the best possible results. From deciding on the method, to quality control, to optimizing specificity - we have important tips and suggestions for you.
Problems with non-specific bands after your PCR? Or, the PCR does not work, but you find lots of amplified primer dimers? No desire for hectic and cooling when pipetting your PCR and the following steps? Looking for reproducible results summer and winter? Then simply use a hot-start PCR.
Maybe you already know it: Even if you did not make a mistake when pipetting your PCR, the results can sometimes be frustrating and disappointing. But why? Conventional DNA polymerases are already active at room temperature - and this can lead to many "falsely amplified" templates, false-positive results and low yield.
The world of genomics and molecular biology has taken a breathtaking leap into the future in recent years. In addition to "high fidelity" enzymes, "highly discriminating" enzymes such as the highly selective SNP DNA polymerases from Genaxxon have become the superstars of the laboratory. These enzymes are specifically modified for highly selective PCR tests and mutation detection - they help you when you want to find "the needle in the haystack".
Since the isolation of the first Taq DNA polymerase in 1976, PCR has become an integral part of the molecular biology laboratory. Of course, methods, reagents and protocols have evolved. The goal is clear: to make it better, faster, cheaper, and easier. Every day, lab technicians, students and scientists around the world spend countless hours pipetting and preparing PCR experiments. This makes it even more frustrating when errors and contamination only become apparent in the final step of the PCR workflow.

But how can the work in the PCR laboratory be optimised to ultimately minimise sources of error and at the same time save time, money, and nerves? We at Genaxxon would like to shed some light into the darkness of the laboratory and show how our new products around the Red Mastermix Fluoro (2X) can help to optimise your PCR workflow along a typical PCR workflow.

Do you already work with multiplex qPCR? And do you appreciate the error, cost and time savings of the multiplex qPCR method? But does your multiplex assay already deliver the best possible results? Specific quality and service features are crucial for you to perform multiplex qPCR accurately and, above all, conveniently. Thanks to the newly developed Taq DNA polymerase in our 5X qPCR Multiplex MasterMix, Genaxxon bioscience offers a solution that guarantees robust PCR performance for a wide range of different applications.
Have you been interrupted while pipetting and forget which well you have already pipetted into? Pipetting loading buffer takes too long? The DNA sample is not completely clean? Then use Genaxxon's Red MasterMix for your PCR - guaranteed high specificity for the best results in your lab. Here are 4 good reasons why you should use Genaxxon's Red MasterMix to simplify your lab work without sacrificing precision.
How to save money using SafeGel from Genaxxon as DNA staining dye for gel electrophoresis
Everything seemed to be easier in the past. At least that’s true when it comes to staining DNA in agarose gels. Ethidium bromide was the agent of choice, despite its mutagenic effect and costly disposal. Today, one is spoilt for choice. But which DNA staining dye is the right one? GelRed® as an alternative is non-toxic and has a high sensitivity, but the price makes one cringe.
The G2 DNA/RNA enhancer shows a significant increase of DNA extraction efficiency using the G2 DNA/RNA Enhancer in combination with 7 commercial kits intended for extraction of DNA from soils.
Are you having trouble isolating microbial DNA or RNA from soil samples, environmental or wastewater samples? Poor accessibility of DNA samples, coupled with low biomass and a variety of inhibitory factors make isolation and further work such as sequencing difficult.

Are you interested in how you can significantly increase your yield of DNA or RNA by a simple additional step in the isolation process?
Genaxxon now offers you a simple solution to your problems - the G2 DNA/RNA Enhancer.
Are you looking for customized cell culture media (custom made media), for customized peptides (custom made peptides) or do you need an amino acid analysis? But you lack the personnel, the time, or the suitable equipment? Then outsource this work to us - we will gladly step in and provide you with a solution exactly according to your wishes.

Whether you need a standard agarose, a high-resolution agarose or a low-melting agarose in your laboratory depends on the size of the DNA or RNA fragments you want to separate electrophoretically, or whether other analytical methods such as blotting, PFGE, in-gel applications, plaque assays or immunodiffusion are to be used.

Do you deal with cloning, sequencing, NGS applications, SNP analyses or mutagenesis in your daily laboratory routine and do you need sequence-exact DNA amplicons ...

RT-PCR: A tale of unfolding the gene regulation

The popularity of RT-PCR or Reverse Transcription PCR has grown tremendously during Coronavirus pandemic and likewise is true for mRNA technology ...

Master of RT qPCR : Genaxxon mastermix

To achieve successful qPCR result is as important as it is to have a reliable sample to work with.
Aus diesem Grund bietet GENAXXON Ihnen eine breitgefächerte Auswahl von Mastermixen aus einer Hand an, mit denen die verschiedensten Fragestellungen und Projekte bearbeitet werden können. Daraus können Sie ganz einfach den passenden Mastermix für Ihre Forschung ...
Time saving, better control on the reaction, high throughput screening of the samples, exclusion of laborious pipetting steps, etc has been made possible by mastermixes offered by various companies around the world. So, what is so special about GENAXXON RedTaq MasterMix (2x),

SNP genotyping and its applications

Progress in the field of SNP genotyping and its applications, from the perspective of plant breeding and agriculture

What are molecular beacons?

Definition, mechanism of work, applications - advantages ...

Fast Cycling Real-Time PCR Conditions

Study of fast Cycling Real-Time PCR Conditions using GENAXXON MasterMixes

Various ways to optimize RNA isolation

When in need for optimising RNA isolation, you shall know GENAXXON and its possibilities
The struggle with understanding various types of PCR is real but we can help you understand it ! Trust us!!!

“Seal the deal” with Gel Electrophoresis!!!

Gel electrophoresis is the technique where DNA molecules move through the, agarose gel-based on their different mass to charge ratio.
So we made a troubleshooting guide only for you!!

Is SARS-COV-2 biased?

Wondering why some countries are more intensely hit by COVID-19 and some not? Could it be different strategies adopted by their government, quarantine, different genetic population herd immunity? Or could the answer lie within the virus itself?

Do you know how vaccines are made?

Researchers first identify the surface molecules like proteins or sugars which are used by the viruses to first contact the human cells. These molecules are then analyzed for if they can be used to produce an immune response. This is not possible unless the genetic sequence of the virus must be known.

Know your pathogens!

We are surrounded by microbes or microorganisms but not all of them are harmful. Actually some are beneficial for us and harmful microbes are called pathogens. Let Genaxxon help you know them better!

Why is next generation sequencing called so?

Sanger sequencing technology was the first generation of sequencing and helped provide basic knowledge about genome sequencing but didn’t have the potential for its further improvement. Hence a new technological jump in sequencing expertise was required. That’s when Next Generation Sequencing techniques came into existence and revolutionized our entire capability of genome sequencing.
Extraction and isolation of viral RNA is the first step in the detection and testing of viral RNA by real-time RT-PCR. Here we compare different methods and protocols for extraction of viral RNA.
Nested RT-PCR and real-time RT-PCR are the most common methods for the analysis of viral infections (see Robert Koch Institute >). In contrast to examinations with ELISA or IFA, this can be done at an earlier stage of infection.

Another secret of SARS-COV-2 revealed!

Extraction and isolation of viral RNA is the first step in the detection and testing of viral RNA by real-time RT-PCR. Here we compare different methods and protocols for extraction of viral RNA.
Nested RT-PCR and real-time RT-PCR are the most common methods for the analysis of viral infections (see Robert Koch Institute >). In contrast to examinations with ELISA or IFA, this can be done at an earlier stage of infection.
A special regulatory strategy of genome translation in RNA viruses and bacterias is mediated via RNA thermometers. RNA thermometers (RNATs) are secondary structures in RNA sequences that are essential for the virulence of the organism. However the base pairing in the RNA thermometers can be altered due to various factors like the thermal denaturation.

RNA thermometers provide a degree in virulence!

A special regulatory strategy of genome translation in RNA viruses and bacterias is mediated via RNA thermometers. RNA thermometers (RNATs) are secondary structures in RNA sequences that are essential for the virulence of the organism. However the base pairing in the RNA thermometers can be altered due to various factors like the thermal denaturation.

Coronavirus motto- Keep mutating!

With the virus already claiming the lives of 1,873 people and infecting 73,333 people around the globe, there is an urgent need to have a strong grip on the virus. Scientists overall are publishing new information in this regard almost every day, one of which is that 2019-nCoV uses the same ACE2 protein to enter the host cells as SARS coronavirus did.

Map the Epitopes like a boss!

With humankind facing a new virus outbreak every now and then, everyone is in the race to develop therapeutics. Out of all the available methods to do so involves knowing the interacting proteins of the pathogen and hosts. The method that uses this approach is known as Epitope Mapping and involves two general strategies.

Decoding the Wuhan coronavirus

The outbreak of a novel coronavirus in Wuhan, China was first reported on 31 December 2019. Just one month later, the World Health Organization has declared the virus a Public Health Emergency of International Concern.
MicroRNAs play a pivotal role in shaping cell phenotype. However, until recently, there were no effective methods to observe miRNA expression within an individual cell, within complex animal tissues, without introducing technical and/or biological artefacts.
Tags: SNPs, miRNA
The human body is colonized by microorganisms of diverse phylogenetic origins, principally bacteria, archaea, eukaryote, and viruses. In health, specific microbes exist in a symbiotic relationship with their human host, where they contribute essential vitamins and nutrients, prime the human immune system to recognize “non-self” antigens, and produce useful anti-inflammatory compounds that deter disease-causing microbes.
Tags: microbiome, SNPs, IBD
Non-protein-coding RNAs (ncRNAs) are expressed in viruses, archaea, prokaryotes and eukaryotes. They fulfil vital roles in the regulation of chromatin architecture, epigenetic memory, transcription, RNA splicing, editing and turnover. To date, at least 18 distinct types of ncRNA have been identified, which are classified according to their size, function, subcellular localization and target specificity.
Here, we focus on two approaches to the safe and efficient design and delivery of CRISPR/Cas9 gene editing technology.
The development of novel antibacterial drugs, such as synthetic venom antimicrobial peptides (AMPs), is a positive step towards overcoming antimicrobial drug resistance.
To date, several different CRISPR/Cas systems have been isolated and classified according to their molecular structure, substrate specificity and the conditions that support their optimum activity.

Alzheimer Peptide with successful phase I trial

Priavoid, a company based in Jülich (Germany), has successfully completed a Phase I project with peptides consisting of D-amino acids instead of the natural occuring L-configuration.
In the articles below you will find the authors already relying on the Genaxxon products. We say thank you to these authors, trusting in our service. Let us convince you as well.
Chinese scientists have succeeded in changing the genetic material of pigs so that they are resistant to the CSF virus. A combination of the CRISPR / Cas9 method and the RNA interference (RNAi) technology was used.
Dog`s and cat`s allergy and exclusion diets are often contaminated with foreign proteins and fats. Small amounts of a potential allergen (such as protein of a certain species) in feed are often sufficient to trigger allergic reactions.
Cell-penetrating peptides (CPPs), also known as protein transduction domains (PTDs), are typically 5-30 amino acid residues in length. Whether they occur naturally or are synthetic, CPPs are designed to breach cell membranes and deliver bioactive cargo to the intracellular space.
Wine production, while considered an art form, owes an increasing debt to the latest molecular genetic technologies. Not only do these facilitate batch-to-batch consistency, they also enable the generation of novel strains of grape microflora. While the choice of grapes is important, so too is the interplay between extrinsic and intrinsic factors, and the technological decisions made at every step in the process. Together, these determine whether the finished product will be classed as a “vintage wine”, a “quaffing wine”, or “vinegar”.
What are the differences between proteins, peptides and amino acids and and how did these molecules originally come about?

Proteins - Peptides - Amino acids: What is a Peptide?

What is a peptide and how is it formed in nature respective a living cell? What are the applications of peptides in diagnostic and medicine?
Peptides show an ever increasing interest in specific therapeutics or for coating surfaces
Short description of different protein analysis / characterisation methods as Edman-Sequencing, Intact Mass Determination, Amino acid analysis, Post-translational Modifications or Disulfide Bridge Analysis.
Standard PCR amplification once required often laborious DNA extraction, purification, end-stage processing and sample set-ups, using relatively primitive instruments and complex protocols. The notion that 35 cycles of PCR amplification could be completed in under 25 minutes, without compromising precision or accuracy, was considered a distant prospect.
SNP PolTaq DNA polymerase for better Sake taste and flavor: SNP PolTaq for qPCR for the unambiguous identification of point mutations in yeast!
Read more about a recent case report from Japan.
Point mutations are the cause of many hereditary diseases and are considered risk factors, for example, for Alzheimer's Disease.
One of the main causes of many hereditary diseases could not be resolved precisely enough by mid-2016, even with the "super tool" CRISPR / Cas9: the point mutation.

Sustainability at Genaxxon

We at Genaxxon bioscience are aware of our corporate responsibility. Our philosophy is to practice sustainability whenever possible. The following are some examples of the many changes that we have implemented successfully.
Nowadays there are many different suppliers and systems for polymerase chain reaction (PCR) and of its derivative techniques, quantitative, or real-time, PCR (qPCR) and reverse transcription PCR (RT-PCR). Competition gives you the opportunity to simplify diagnostics and to make research more financially favorable.
We are proud to inform you that Genaxxon bioscience has been successfully certified according TÜV ISO 9001: 2015!

PCR Beads Ready-To-Use lyophilized - LyoBeads:

Get the same reliable performance in your realtime PCR as before, now with a two-year shelf life at room temperature!

Pre-formulated, freeze-dried realtime PCR master mix in bead format. No need to store the PCR Master Mix (Beads) in the refrigerator or freezer. No unnecessary freeze-thaw cycles. No wasted time for thawing the master mix each time!
The qPCR and Digital PCR Conference started with lectures on the reliability of qPCR and ddPCR, from their technical limitations to the interpretation of results to errors in published data with a very interesting presentation by Prof. Stephen Bustin.

miRNAs in Gene Expression

miRNAs occur naturally in animal and plant organisms. They are highly conserved and play an important role in gene regulation, especially in gene silencing. They have an important function in cell differentiation and dedifferentiation, for example in female fertility and preimplantation development.

Digital PCR (dPCR)

Doing digital PCR means to carry out a PCR reaction with only one single amplification step nonetheless resulting in absolute/quantitative values. For dPCR, in contrast to conventional real-time PCR (qPCR), no standard curves, calculating of Ct-values or standard references are required because the single amplification step is performed to obtain a pure yes (positive) or no (negative) answer.
By using the new HotScriptase 2X Cell master mix from Genaxxon RNA viruses (e.g. Zika virus, dengue virus, yellow fever virus) can be detected directly from body fluids as blood, salvia or semen without isolating or purifying RNA prior to RT-PCR.

SNP - Allele Specific Discrimination

There's no accounting for taste.
Cilantro (Coriander): some people love it in their food, some hate it. Here we are detecting a genomic SNP (rs72921001) in HeLa genomic DNA. This SNP is reported to be close to a number of genes coding for olfactory receptors. (Reference: Eriksson N. et al. (2012), "A genetic variant near olfactory receptor genes influences cilantro preference.")

Considering, that only the C-allele specific primer is extended and yielding in a specific amplicon, we can conclude a genetic predisposition in disliking cilantro, as this SNP is significantly associated with detecting a soapy taste to cilantro.

CRISPR/Cas9 optimized workflow

The gRNA ("guide RNA") of the CRISPR/Cas9 system is a short synthetic RNA composed of a sequence (tracrRNA = non-target specific sequence) necessary to bind to the Cas9 protein and a gene specific ca. 20 nucleotide “spacer” or “targeting” sequence (crRNA) which defines the genomic target to be modified. We show in a scheme (according to Liang et al., 2015) an optimized workflow for cell analyzes with the CRISPR/Cas9 system from the theoretical design of the gRNA via transcription and transfection to the analysis of the experimentally obtained data within shortest time with special tips for products to use.

CRISPR/Cas9 Methods

Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) systems are revolutionizing the field of genome editing providing a powerful tool for disrupting the gene of interest. Able to achieve highly flexible and specific targeting, CRISPR-Cas systems can be modified and redirected being a powerful tool for genome editing in many species, including mammalian cells.

Basics of quantitative RT-PCR

Real-time RT-PCR compared to end-point detection methods.
Description of different methods for gene expression quantitation by real-time RT-PCR and end-point detection/quantification techniques.

results of our survey in 2015

Results of our online survey

We would like to thank our customers for the great response in answering our online survey and the excellent ratings for our service and our quality and price-performance ratio.
Our new
HotScriptase RT Cell Mastermix:
For the cDNA synthesis directly from cells, there is no need for a time-consuming and expensive cell lysis or RNA purification any more.
Every single pipetting step and each tube that has to be opened increases the risk of contamination. So far, reverse transcription involved the risk of contaminating the valuable sample by many pipetting steps. With our new all-in-one enzyme > HotScriptase RT Polymerase the risk of contamination is clearly minimized with the zero-step protocol. Moreover, not to mention the considerable time saving.

quantitative RT-PCR (engl.)

Genaxxon bioscience offers new an innovative "one-step" method to transcribe mRNA into DNA with simultaneous quantification of DNA. With the new HotScriptase enzyme and the HotScriptase master mixes the RNA has only to be added to the PCR reaction and the PCR program simply has to be started.

MHC-I and MHC-II Peptides

Genaxxon bioscience offers different Peptides for examination on binding studies of MHC-Class-I and MHC-Class-II complexes. The advantage of the Genaxxon bioscience peptides is their defined and known amino acid sequence and the high purity, which enables an exact interpretation of results, without ambiguity that might be caused by impurities.