The G2 DNA/RNA enhancer shows a significant increase of DNA extraction efficiency using the G2 DNA/RNA Enhancer in combination with 7 commercial kits intended for extraction of DNA from soils.
Are you having trouble isolating microbial DNA or RNA from soil samples, environmental or wastewater samples? Poor accessibility of DNA samples, coupled with low biomass and a variety of inhibitory factors make isolation and further work such as sequencing difficult.
Are you interested in how you can significantly increase your yield of DNA or RNA by a simple additional step in the isolation process?
Genaxxon now offers you a simple solution to your problems - the G2 DNA/RNA Enhancer.
Are you having trouble isolating microbial DNA or RNA from soil samples, environmental or wastewater samples? Poor accessibility of DNA samples, coupled with low biomass and a variety of inhibitory factors make isolation and further work such as sequencing difficult.
Are you interested in how you can significantly increase your yield of DNA or RNA by a simple additional step in the isolation process?
Genaxxon now offers you a simple solution to your problems - the G2 DNA/RNA Enhancer.
Are you looking for customized cell culture media (custom made media), for customized peptides (custom made peptides) or do you need an amino acid analysis? But you lack the personnel, the time, or the suitable equipment? Then outsource this work to us - we will gladly step in and provide you with a solution exactly according to your wishes.
Whether you need a standard agarose, a high-resolution agarose or a low-melting agarose in your laboratory depends on the size of the DNA or RNA fragments you want to separate electrophoretically, or whether other analytical methods such as blotting, PFGE, in-gel applications, plaque assays or immunodiffusion are to be used.
Do you deal with cloning, sequencing, NGS applications, SNP analyses or mutagenesis in your daily laboratory routine and do you need sequence-exact DNA amplicons ...
Isolate miRNA, RNA and DNA with only one kit from GENAXXON!
Spin columns and Midi columns for nucleic acid purification separate from the purification kit - a sustainable and environmentally friendly offer from GENAXXON!
The popularity of RT-PCR or Reverse Transcription PCR has grown tremendously during Coronavirus pandemic and likewise is true for mRNA technology ...
Due to the large number of tests, however, the question arises: which test fits my question? This starts with the...
To achieve successful qPCR result is as important as it is to have a reliable sample to work with.
Aus diesem Grund bietet GENAXXON Ihnen eine breitgefächerte Auswahl von Mastermixen aus einer Hand an, mit denen die verschiedensten Fragestellungen und Projekte bearbeitet werden können. Daraus können Sie ganz einfach den passenden Mastermix für Ihre Forschung ...
Aus diesem Grund bietet GENAXXON Ihnen eine breitgefächerte Auswahl von Mastermixen aus einer Hand an, mit denen die verschiedensten Fragestellungen und Projekte bearbeitet werden können. Daraus können Sie ganz einfach den passenden Mastermix für Ihre Forschung ...
Time saving, better control on the reaction, high throughput screening of the samples, exclusion of laborious pipetting steps, etc has been made possible by mastermixes offered by various companies around the world. So, what is so special about GENAXXON RedTaq MasterMix (2x),
Progress in the field of SNP genotyping and its applications, from the perspective of plant breeding and agriculture
Definition, mechanism of work, applications - advantages ...
Study of fast Cycling Real-Time PCR Conditions using GENAXXON MasterMixes
When in need for optimising RNA isolation, you shall know GENAXXON and its possibilities
One step and Two step RT-PCR
The struggle with understanding various types of PCR is real but we can help you understand it ! Trust us!!!
Gel electrophoresis is the technique where DNA molecules move through the, agarose gel-based on their different mass to charge ratio.
So we made a troubleshooting guide only for you!!
So we made a troubleshooting guide only for you!!
Wondering why some countries are more intensely hit by COVID-19 and some not? Could it be different strategies adopted by their government, quarantine, different genetic population herd immunity? Or could the answer lie within the virus itself?
Researchers first identify the surface molecules like proteins or sugars which are used by the viruses to first contact the human cells. These molecules are then analyzed for if they can be used to produce an immune response. This is not possible unless the genetic sequence of the virus must be known.
We are surrounded by microbes or microorganisms but not all of them are harmful. Actually some are beneficial for us and harmful microbes are called pathogens. Let Genaxxon help you know them better!
Sanger sequencing technology was the first generation of sequencing and helped provide basic knowledge about genome sequencing but didn’t have the potential for its further improvement. Hence a new technological jump in sequencing expertise was required. That’s when Next Generation Sequencing techniques came into existence and revolutionized our entire capability of genome sequencing.
Extraction and isolation of viral RNA is the first step in the detection and testing of viral RNA by real-time RT-PCR. Here we compare different methods and protocols for extraction of viral RNA.
Nested RT-PCR and real-time RT-PCR are the most common methods for the analysis of viral infections (see Robert Koch Institute >). In contrast to examinations with ELISA or IFA, this can be done at an earlier stage of infection.
Nested RT-PCR and real-time RT-PCR are the most common methods for the analysis of viral infections (see Robert Koch Institute >). In contrast to examinations with ELISA or IFA, this can be done at an earlier stage of infection.
Extraction and isolation of viral RNA is the first step in the detection and testing of viral RNA by real-time RT-PCR. Here we compare different methods and protocols for extraction of viral RNA.
Nested RT-PCR and real-time RT-PCR are the most common methods for the analysis of viral infections (see Robert Koch Institute >). In contrast to examinations with ELISA or IFA, this can be done at an earlier stage of infection.
Nested RT-PCR and real-time RT-PCR are the most common methods for the analysis of viral infections (see Robert Koch Institute >). In contrast to examinations with ELISA or IFA, this can be done at an earlier stage of infection.
A special regulatory strategy of genome translation in RNA viruses and bacterias is mediated via RNA thermometers. RNA thermometers (RNATs) are secondary structures in RNA sequences that are essential for the virulence of the organism. However the base pairing in the RNA thermometers can be altered due to various factors like the thermal denaturation.
A special regulatory strategy of genome translation in RNA viruses and bacterias is mediated via RNA thermometers. RNA thermometers (RNATs) are secondary structures in RNA sequences that are essential for the virulence of the organism. However the base pairing in the RNA thermometers can be altered due to various factors like the thermal denaturation.
With the virus already claiming the lives of 1,873 people and infecting 73,333 people around the globe, there is an urgent need to have a strong grip on the virus. Scientists overall are publishing new information in this regard almost every day, one of which is that 2019-nCoV uses the same ACE2 protein to enter the host cells as SARS coronavirus did.
With humankind facing a new virus outbreak every now and then, everyone is in the race to develop therapeutics. Out of all the available methods to do so involves knowing the interacting proteins of the pathogen and hosts. The method that uses this approach is known as Epitope Mapping and involves two general strategies.
The outbreak of a novel coronavirus in Wuhan, China was first reported on 31 December 2019. Just one month later, the World Health Organization has declared the virus a Public Health Emergency of International Concern.
MicroRNAs play a pivotal role in shaping cell phenotype. However, until recently, there were no effective methods to observe miRNA expression within an individual cell, within complex animal tissues, without introducing technical and/or biological artefacts.
The human body is colonized by microorganisms of diverse phylogenetic origins, principally bacteria, archaea, eukaryote, and viruses. In health, specific microbes exist in a symbiotic relationship with their human host, where they contribute essential vitamins and nutrients, prime the human immune system to recognize “non-self” antigens, and produce useful anti-inflammatory compounds that deter disease-causing microbes.
Non-protein-coding RNAs (ncRNAs) are expressed in viruses, archaea, prokaryotes and eukaryotes. They fulfil vital roles in the regulation of chromatin architecture, epigenetic memory, transcription, RNA splicing, editing and turnover. To date, at least 18 distinct types of ncRNA have been identified, which are classified according to their size, function, subcellular localization and target specificity.
Here, we focus on two approaches to the safe and efficient design and delivery of CRISPR/Cas9 gene editing technology.
The development of novel antibacterial drugs, such as synthetic venom antimicrobial peptides (AMPs), is a positive step towards overcoming antimicrobial drug resistance.
To date, several different CRISPR/Cas systems have been isolated and classified according to their molecular structure, substrate specificity and the conditions that support their optimum activity.
Priavoid, a company based in Jülich (Germany), has successfully completed a Phase I project with peptides consisting of D-amino acids instead of the natural occuring L-configuration.
In the articles below you will find the authors already relying on the Genaxxon products. We say thank you to these authors, trusting in our service. Let us convince you as well.
Chinese scientists have succeeded in changing the genetic material of pigs so that they are resistant to the CSF virus. A combination of the CRISPR / Cas9 method and the RNA interference (RNAi) technology was used.
Dog`s and cat`s allergy and exclusion diets are often contaminated with foreign proteins and fats. Small amounts of a potential allergen (such as protein of a certain species) in feed are often sufficient to trigger allergic reactions.
Cell-penetrating peptides (CPPs), also known as protein transduction domains (PTDs), are typically 5-30 amino acid residues in length. Whether they occur naturally or are synthetic, CPPs are designed to breach cell membranes and deliver bioactive cargo to the intracellular space.
Wine production, while considered an art form, owes an increasing debt to the latest molecular genetic technologies. Not only do these facilitate batch-to-batch consistency, they also enable the generation of novel strains of grape microflora. While the choice of grapes is important, so too is the interplay between extrinsic and intrinsic factors, and the technological decisions made at every step in the process. Together, these determine whether the finished product will be classed as a “vintage wine”, a “quaffing wine”, or “vinegar”.
What are the differences between proteins, peptides and amino acids and and how did these molecules originally come about?
What is a peptide and how is it formed in nature respective a living cell? What are the applications of peptides in diagnostic and medicine?
Peptides show an ever increasing interest in specific therapeutics or for coating surfaces
Short description of different protein analysis / characterisation methods as Edman-Sequencing, Intact Mass Determination, Amino acid analysis, Post-translational Modifications or Disulfide Bridge Analysis.
Standard PCR amplification once required often laborious DNA extraction, purification, end-stage processing and sample set-ups, using relatively primitive instruments and complex protocols. The notion that 35 cycles of PCR amplification could be completed in under 25 minutes, without compromising precision or accuracy, was considered a distant prospect.
SNP PolTaq DNA polymerase for better Sake taste and flavor: SNP PolTaq for qPCR for the unambiguous identification of point mutations in yeast!
Read more about a recent case report from Japan.
Read more about a recent case report from Japan.
Point mutations are the cause of many hereditary diseases and are considered risk factors, for example, for Alzheimer's Disease.
One of the main causes of many hereditary diseases could not be resolved precisely enough by mid-2016, even with the "super tool" CRISPR / Cas9: the point mutation.
One of the main causes of many hereditary diseases could not be resolved precisely enough by mid-2016, even with the "super tool" CRISPR / Cas9: the point mutation.
We at Genaxxon bioscience are aware of our corporate responsibility. Our philosophy is to practice sustainability whenever possible. The following are some examples of the many changes that we have implemented successfully.
Nowadays there are many different suppliers and systems for polymerase chain reaction (PCR) and of its derivative techniques, quantitative, or real-time, PCR (qPCR) and reverse transcription PCR (RT-PCR). Competition gives you the opportunity to simplify diagnostics and to make research more financially favorable.
We are proud to inform you that Genaxxon bioscience has been successfully certified according TÜV ISO 9001: 2015!
Get the same reliable performance in your realtime PCR as before, now with a two-year shelf life at room temperature!
Pre-formulated, freeze-dried realtime PCR master mix in bead format. No need to store the PCR Master Mix (Beads) in the refrigerator or freezer. No unnecessary freeze-thaw cycles. No wasted time for thawing the master mix each time!
Pre-formulated, freeze-dried realtime PCR master mix in bead format. No need to store the PCR Master Mix (Beads) in the refrigerator or freezer. No unnecessary freeze-thaw cycles. No wasted time for thawing the master mix each time!
The qPCR and Digital PCR Conference started with lectures on the reliability of qPCR and ddPCR, from their technical limitations to the interpretation of results to errors in published data with a very interesting presentation by Prof. Stephen Bustin.
miRNAs occur naturally in animal and plant organisms. They are highly conserved and play an important role in gene regulation, especially in gene silencing. They have an important function in cell differentiation and dedifferentiation, for example in female fertility and preimplantation development.
Doing digital PCR means to carry out a PCR reaction with only one single amplification step nonetheless resulting in absolute/quantitative values. For dPCR, in contrast to conventional real-time PCR (qPCR), no standard curves, calculating of Ct-values or standard references are required because the single amplification step is performed to obtain a pure yes (positive) or no (negative) answer.
By using the new HotScriptase 2X Cell master mix from Genaxxon RNA viruses (e.g. Zika virus, dengue virus, yellow fever virus) can be detected directly from body fluids as blood, salvia or semen without isolating or purifying RNA prior to RT-PCR.
There's no accounting for taste.
Cilantro (Coriander): some people love it in their food, some hate it. Here we are detecting a genomic SNP (rs72921001) in HeLa genomic DNA. This SNP is reported to be close to a number of genes coding for olfactory receptors. (Reference: Eriksson N. et al. (2012), "A genetic variant near olfactory receptor genes influences cilantro preference.")
Considering, that only the C-allele specific primer is extended and yielding in a specific amplicon, we can conclude a genetic predisposition in disliking cilantro, as this SNP is significantly associated with detecting a soapy taste to cilantro.
Cilantro (Coriander): some people love it in their food, some hate it. Here we are detecting a genomic SNP (rs72921001) in HeLa genomic DNA. This SNP is reported to be close to a number of genes coding for olfactory receptors. (Reference: Eriksson N. et al. (2012), "A genetic variant near olfactory receptor genes influences cilantro preference.")
Considering, that only the C-allele specific primer is extended and yielding in a specific amplicon, we can conclude a genetic predisposition in disliking cilantro, as this SNP is significantly associated with detecting a soapy taste to cilantro.
The gRNA ("guide RNA") of the CRISPR/Cas9 system is a short synthetic RNA composed of a sequence (tracrRNA = non-target specific sequence) necessary to bind to the Cas9 protein and a gene specific ca. 20 nucleotide “spacer” or “targeting” sequence (crRNA) which defines the genomic target to be modified. We show in a scheme (according to Liang et al., 2015) an optimized workflow for cell analyzes with the CRISPR/Cas9 system from the theoretical design of the gRNA via transcription and transfection to the analysis of the experimentally obtained data within shortest time with special tips for products to use.
Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) systems are revolutionizing the field of genome editing providing a powerful tool for disrupting the gene of interest. Able to achieve highly flexible and specific targeting, CRISPR-Cas systems can be modified and redirected being a powerful tool for genome editing in many species, including mammalian cells.
Real-time RT-PCR compared to end-point detection methods.
Description of different methods for gene expression quantitation by real-time RT-PCR and end-point detection/quantification techniques.
Description of different methods for gene expression quantitation by real-time RT-PCR and end-point detection/quantification techniques.
Results of our online survey
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We would like to thank our customers for the great response in answering our online survey and the excellent ratings for our service and our quality and price-performance ratio.
Our new
HotScriptase RT Cell Mastermix:
For the cDNA synthesis directly from cells, there is no need for a time-consuming and expensive cell lysis or RNA purification any more.
HotScriptase RT Cell Mastermix:
For the cDNA synthesis directly from cells, there is no need for a time-consuming and expensive cell lysis or RNA purification any more.
Every single pipetting step and each tube that has to be opened increases the risk of contamination. So far, reverse transcription involved the risk of contaminating the valuable sample by many pipetting steps. With our new all-in-one enzyme > HotScriptase RT Polymerase the risk of contamination is clearly minimized with the zero-step protocol. Moreover, not to mention the considerable time saving.
Genaxxon bioscience offers new an innovative "one-step" method to transcribe mRNA into DNA with simultaneous quantification of DNA. With the new HotScriptase enzyme and the HotScriptase master mixes the RNA has only to be added to the PCR reaction and the PCR program simply has to be started.
Genaxxon bioscience offers different Peptides for examination on binding studies of MHC-Class-I and MHC-Class-II complexes. The advantage of the Genaxxon bioscience peptides is their defined and known amino acid sequence and the high purity, which enables an exact interpretation of results, without ambiguity that might be caused by impurities.