PCR and related Products

Uracil DNA Glycosylase (UDG), also called uracil N-glycosylase (UNG), catalyzes the release of uracil from uracil-containing DNA. The UDG/UNG from Genaxxon is a highly purified, recombinant enzyme isolated from E. coli with a mass of 26 kDa. UDG efficiently hydrolyzes single- and double-stranded DNA containing uracil, except for oligomers with 6 or fewer bases. Therefore, uracil-DNA glycosylase (UDG) is used wherever it is necessary to avoid the risk of DNA carryover (contamination) during PCR. In order to be able to hydrolyze DNA by UDG/UNG you do need dUTP > in your PCR reactions. Storage buffer: 20mM Tris HCl (pH 8.0) 50mM NaCl 0.1mM EDTA 50% Glycerin Reaction buffer (1X): 20 mM Tris HCl (pH 8.0) 1 mM EDTA

5X Multiplex PCR Mastermix for robust PCR with all components for rapid, sensitive and reproducible quantification of DNA. The optimized DNA polymerase and an optimized buffer including our ultrapure dNTPs are key components of the ready to use mix. A hot-start formulation of the included DNA polymerase prevents false amplification during the reaction setup. Our 5X qPCR master mix will function with a wide range of templates including human-, mammal-, and plant-derived samples. Features:- 5-time concentrate for more variability in primer- and probe volumes- amplification of different targets in a single PCR tube (tested for up to 4 targets).- fast start function due to a hot-start aptamer formulation.- the hot-start formulation of the included DNA polymerase prevents false amplification during the reaction setup- the optimized buffer includes our ultrapure dNTPs Our new lyophilized Multiplex master mix for fast and easy multiplexing minimizes the need for optimization and makes the development of multiplex PCR assays fast and easy. Our new 5-fold multiplex mastermix minimizes the need for optimization and therefore makes the development and establishment of multiplex PCR easier and faster.  Read now in our blog why the 5x qPCR Multiplex MasterMix is the optimal choice for your multiplex qPCR. Our Standard Agarose LE > and especially our high resolution Agarose Tiny > are ideally suited for the subsequent electrophoresis analysis. Other realtime master mixes for your realtime PCR experiments can be found here >.> Examples of Multiplex applications: F. Javier Pérez-Pérez and Nancy D. Hanson, Detection of Plasmid-Mediated AmpC β-Lactamase Genes in Clinical Isolates by Using Multiplex PCR, J. Clin. Microbiol. June 2002 vol. 40 no. 6 2153-2162. doi: 10.1128/JCM.40.6.2153-2162.2002. Tamara B. Souzaa, Diego M. Lozerb, Sônia M. S. Kitagawab, Liliana C. Spanob, Neusa P. Silvac and Isabel C. A. Scaletskya, Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli. J. Clin. Microbiol. March 2013 vol. 51 no. 3 1031-1033, doi: 10.1128/JCM.02478-12.

Lyophilized 5X Multiplex PCR Mastermix for robust PCR with all components for rapid, sensitive and reproducible quantification of DNA. The optimized DNA polymerase and an optimized buffer including our ultrapure dNTPs are key components of the ready to use mix. A hot-start formulation of the included DNA polymerase prevents false amplification during the reaction setup. Our 5X qPCR master mix will function with a wide range of templates including human-, mammal-, and plant-derived samples. Features:- 5-time concentrate for more variability in primer- and probe volumes- amplification of different targets in a single PCR tube (tested for up to 4 targets).- the hot-start formulation of the included DNA polymerase prevents false amplification during the reaction setup- the optimized buffer includes our ultrapure dNTPs Our new lyophilized Multiplex master mix for fast and easy multiplexing minimizes the need for optimization and makes the development of multiplex PCR assays fast and easy. Our new 5-fold multiplex mastermix minimizes the need for optimization and therefore makes the development and establishment of multiplex PCR easier and faster.  Stability- the lyophilized MasterMix is stable for 3 years, if stored at -20°C.- the lyophilized MasterMix is stable for at least 12 months, if stored after delivery at +15°C to+30°C.- the reconstituted MasterMix is stable for 6 months, if stored at -20°C. Read now in our blog why the 5x qPCR Multiplex MasterMix is the optimal choice for your multiplex qPCR. Our Standard Agarose LE > and especially our high resolution Agarose Tiny > are ideally suited for the subsequent electrophoresis analysis. Other realtime master mixes for your realtime PCR experiments can be found here >. Examples of Multiplex applications:F. Javier Pérez-Pérez and Nancy D. Hanson, Detection of Plasmid-Mediated AmpC β-Lactamase Genes in Clinical Isolates by Using Multiplex PCR, J. Clin. Microbiol. June 2002 vol. 40 no. 6 2153-2162. doi: 10.1128/JCM.40.6.2153-2162.2002. Tamara B. Souzaa, Diego M. Lozerb, Sônia M. S. Kitagawab, Liliana C. Spanob, Neusa P. Silvac and Isabel C. A. Scaletskya, Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli. J. Clin. Microbiol. March 2013 vol. 51 no. 3 1031-1033, doi: 10.1128/JCM.02478-12.

Multiplex PCR is a method that enables amplification of two or more amplicons simultaneously in a single reaction tube/reaction. It is widely used in genotyping and different areas of DNA testing in research, forensic and diagnostic laboratories. Our Multiplex HS Mastermix (2X) is an optimized ready-to-use mixture for probe-based assays such as TaqMan®, Beacons and MGBs. It contains a modified fast HotStart Taq DNA Polymerase, dNTPs and MgCl₂ combined in an optimized buffer system for realtime PCR / qPCR applications except primers, probe and template DNA / cDNA.  The HotStart Taq Polymerase is based on the standard Taq DNA polymerase from Genaxxon inactivated by an specific antibody against Taq DNA polymerase which is activated by heat treatment. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity, increased sensitivity and greater yields when compared to standard DNA polymerases, making this enzyme especially well-suited for multiplex PCR. - Amplification of multiple targets in a single tube- All-in-one master mix for convenient multiplexing- High specificity, sensitivity and product yield- Easy reaction setup at room temperature The Multiplex HS Master mix 2X is shipped in aliquots of 1mL. Our Standard Agarose LE > and especially our high resolution Agarose Tiny > are ideally suited for the subsequent electrophoresis analysis. Other realtime master mixes for your realtime PCR experiments can be found here >. Examples of Multiplex applications: F. Javier Pérez-Pérez and Nancy D. Hanson, Detection of Plasmid-Mediated AmpC β-Lactamase Genes in Clinical Isolates by Using Multiplex PCR, J. Clin. Microbiol. June 2002 vol. 40 no. 6 2153-2162. doi: 10.1128/JCM.40.6.2153-2162.2002. Tamara B. Souzaa, Diego M. Lozerb, Sônia M. S. Kitagawab, Liliana C. Spanob, Neusa P. Silvac and Isabel C. A. Scaletskya, Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli. J. Clin. Microbiol. March 2013 vol. 51 no. 3 1031-1033, doi: 10.1128/JCM.02478-12.

Lyophilized (freeze-dried) temperature stable and pre-mixed Hotstart master mix with all necessary components for probe realtime PCR (optimized buffer, dNTPs, Hotstart Taq) for difficult to amplify templates, qPCR and Multiplex applications. No cooling/cooling chain necessary! Our LyoMix and all ingredients are unopened stable at ambient temperature for at least 24 months! Our lyophilized qPCR master mix is also available as LyoBalls pre-portioned in PCR tube strips or PCR plates (M3069 > - 0.1mL tubes or M3070 > - 0.2mL tubes)! Our Hotstart Polymerase guarantees that during setup and the first PCR cycle the enzyme is not active and misprimed primers are not extended. As a result specificity and efficiency are increased by far compared to standard Taq DNA polymerase. Additionally, difficult targets with high GC-content can be amplified. The higher sensitivity improves multiplex PCR results.This lyophilized master mix in powder form is the perfect choice for a fast reaction setup that reduces the time required for pipetting and the possibility for pipetting errors. Just add PCR-grade water, template and primers to reconstitute the master mix and you are all set! Lyophilized realtime PCR master mix:• Hotstart Polymerase• increased specificity• increased sensitivity• for difficult to amplify templates with high GC content• for Multiplex applications• for qPCR• ready-to-useThis lyophilized Hotstart master mix is delivered in powder form that can be easily dispensed. With our high quality dNTPs as Set > (M3015.4100 and M3015.0250) or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.

ProbeMasterMix FAST without ROX is optimised for fast qPCR assays with probes in block systems. The master mix is optimized for fast PCR with short denaturation 2-step cycles. This 2X Mastermix is ready-to-use and contains all components for a successful and reliable qPCR with the exception of primer and template DNA. Advantages of the Hotstart Taq polymerase used in the Genaxxon ProbeMasterMix: Hotstart technology enables setup of PCR mixture at room temperature. short initial denaturation of not more than 2 minutes. optimized for 2-step PCR protocols. No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon Hotstart Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The hotstart formulation inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Probe qPCR master mix without ROX contains all the necessary components in an optimized composition to carry out quantitative PCR. hotstart Taq DNA Polymerase dATP, dCTP, dGTP, dTTP optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1mL simplifies handling and storage (less freeze-thaw cycles per aliquote) This Mastermix is specially suited for the following instruments: BioRad CFX96 Touch™, CFX384 Touch™, CFX Connect™, DNA Engine Opticon® 2, Chromo4™, iCycler iQ™ and My iQ™ , Roche LightCycler® 480, LightCycler® 1536, LightCycler® Nano, LightCycler® 96 and QuantStudio™ instruments, Thermo Scientific™ PikoReal™, Cepheid SmartCycler®, Bio Molecular Systems Mic qPCR cycler, Qiagen Rotor Gene Q, Rotor Gene 6000, MyGo Mini and MyGo Pro. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

ProbeMasterMix without ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains optimised amounts of all ingredients. Multiplex PCR: Applications at Genaxxon and at customers site have shown that the qPCR Probe Mastermix can be used for the simultaneous detection of up to four DNA targets in the same PCR reaction. For further details please refer to the product manual or contact us: info@genaxxon.com. Advantages of the chemically modified SuperHot Taq polymerase used in the Genaxxon ProbeMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Probe qPCR master mix without ROX contains all the necessary components in an optimized composition to carry out quantitative PCR. chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: BioRad CFX96 Touch™, CFX384 Touch™, CFX Connect™, DNA Engine Opticon® 2, Chromo4™, iCycler iQ™ and My iQ™ , Roche LightCycler® 480, LightCycler® 1536, LightCycler® Nano, LightCycler® 96 and QuantStudio™ instruments, Thermo Scientific™ PikoReal™, Cepheid SmartCycler®, Bio Molecular Systems Mic qPCR cycler, Qiagen Rotor Gene Q, Rotor Gene 6000, MyGo Mini and MyGo Pro. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

GreenMasterMix FAST without ROX is optimised for fast qPCR assays without probes in block systems. The master mix is optimized for fast PCR with short denaturation 2-step cycles. This 2X Mastermix is ready-to-use and contains all components for a successful and reliable qPCR with the exception of primer and template DNA. Advantages of the HotStart Taq DNA polymerase used in the Genaxxon GreenMasterMix: Hotstart technology enables setup of PCR mixture at room temperature. short initial denaturing time of not more than 2 minutes No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Green qPCR master mix without ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: Hotstart Taq DNA Polymerase dATP, dCTP, dGTP, dTTP intercalating green fluorescent dye optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: BioRad CFX96 Touch™, CFX384 Touch™, CFX Connect™, DNA Engine Opticon® 2, Chromo4™, iCycler iQ™ and My iQ™ , Roche LightCycler® 480, LightCycler® 1536, LightCycler® Nano, LightCycler® 96 and QuantStudio™ instruments, Thermo Scientific™ PikoReal™, Cepheid SmartCycler®, Bio Molecular Systems Mic qPCR cycler, Qiagen Rotor Gene Q, Rotor Gene 6000, MyGo Mini and MyGo Pro. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

GreenMasterMix without ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains optimised amounts of all ingredients. Advantages of the chemically modified SuperHot Taq polymerase used in the Genaxxon GreenMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Green qPCR master mix without ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP intercalating green fluorescent dye optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: BioRad CFX96 Touch™, CFX384 Touch™, CFX Connect™, DNA Engine Opticon® 2, Chromo4™, iCycler iQ™ and My iQ™ , Roche LightCycler® 480, LightCycler® 1536, LightCycler® Nano, LightCycler® 96 and QuantStudio™ instruments, Thermo Scientific™ PikoReal™, Cepheid SmartCycler®, Bio Molecular Systems Mic qPCR cycler, Qiagen Rotor Gene Q, Rotor Gene 6000, MyGo Mini and MyGo Pro. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

ProbeMasterMix FAST with 50nM ROX is optimised for fast qPCR assays with probes in block systems. The master mix is optimized for fast qPCR with short denaturation 2-step cycles. This 2X Mastermix is ready-to-use and contains all components for a successful and reliable qPCR with the exception of primer and template DNA Advantages of the Hotstart Taq polymerase used in the Genaxxon ProbeMasterMix: Hotstart technology enables setup of PCR mixture at room temperature. short initial denaturation of not more than 2 minutes. optimized for 2-step PCR protocols. No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon Hotstart Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The hotstart formulation inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Probe qPCR master mix with 50nM ROX contains all the necessary components in an optimized composition to carry out quantitative PCR. hotstart Taq DNA Polymerase dATP, dCTP, dGTP, dTTP optimized reaction buffer 50nM ROX as internal passive reference stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) This Mastermix is specially suited for the following instruments: Applied Biosystems® 7500, 7500 Fast and ViiA™ 7, QuantStudio™ instruments, Agilent Mx3000P™, Mx3005P™, Mx4000™ and AriaMx. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

ProbeMasterMix low ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X master mix is ready-to-use and contains optimised amounts of all ingredients. Multiplex PCR: Applications at Genaxxon and at customers site have shown that the qPCR Probe Mastermix can be used for the simultaneous detection of up to four DNA targets in the same PCR reaction. For further details please refer to the product manual or contact us: info@genaxxon.com. Advantages of the Genaxxon ProbeMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Probe qPCR master mix without ROX contains all the necessary components in an optimized composition to carry out quantitative PCR. chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP 50nM ROX optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: Applied Biosystems® 7500, 7500 Fast and ViiA™ 7, QuantStudio™ instruments, Agilent Mx3000P™, Mx3005P™, Mx4000™ and AriaMx. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

GreenMasterMix FAST with 50nM ROX is optimised for realtime PCR assays in block systems. This 2X Mastermix is ready-to-use and contains all components for a successful and reliable qPCR with the exception of primer and template DNA. Advantages of the HotStart Taq DNA polymerase used in the Genaxxon GreenMasterMix: Hotstart technology enables setup of PCR mixture at room temperature. short initial denaturing time of not more than 2 minutes No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Green qPCR master mix with 50nM ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: Hotstart Taq DNA Polymerase dATP, dCTP, dGTP, dTTP intercalating green fluorescent dye optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets 50nM internal passive reference fluorescence dye the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) This Mastermix is specially suited for the following instruments: Applied Biosystems® 7500, 7500 Fast and ViiA™ 7, QuantStudio™ instruments, Agilent Mx3000P™, Mx3005P™, Mx4000™ and AriaMx. Other realtime PCR master mixes from Genaxxon are: M3023 - GreenMastermix No ROX >, M3045 - ProbeMastermix No ROX >, M3011 - GreenMastermix Low ROX >, M3031 - ProbeMastermix Low ROX >, M3052 - GreenMastermix High ROX >, M3010 - ProbeMastermix High ROX >.

GreenMastermix Low ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains optimised amounts of all ingredients. Advantages of the chemically modified SuperHot Taq polymerase used in the Genaxxon GreenMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Green qPCR master mix high ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP intercalating green fluorescent dye 50nM ROX optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: Applied Biosystems® 7500, 7500 Fast and ViiA™ 7, QuantStudio™ instruments, Agilent Mx3000P™, Mx3005P™, Mx4000™ and AriaMx. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

ProbeMasterMix FAST with 500nM  ROX optimised for fast realtime PCR assays in block systems. This master mix is ready-to-use and contains all components for a successful and reliable quantitative PCR with the exception of primer and template DNA. Advantages of the Hotstart Taq polymerase used in the Genaxxon ProbeMasterMix: Hotstart technology enables setup of PCR mixture at room temperature. short initial denaturation of not more than 2 minutes. optimized for 2-step PCR protocols. No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon Hotstart Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The hotstart formulation inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Probe qPCR master mix with 500nM ROX contains all the necessary components in an optimized composition to carry out quantitative PCR. hotstart Taq DNA Polymerase dATP, dCTP, dGTP, dTTP optimized reaction buffer 500nM ROX as internal reference stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) This Mastermix is specially suited for the following instruments: Applied Biosystems® 5700, 7000, 7300, 7700, 7900, 7900 HT, Eppendorf Realplex4, StepOne™ and StepOnePlus™. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

ProbeMastermix High ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains optimised amounts of all ingredients. Multiplex PCR: Applications at Genaxxon and at customers site have shown that the qPCR Probe Mastermix can be used for the simultaneous detection of up to four DNA targets in the same PCR reaction. For further details please refer to the product manual or contact us: info@genaxxon.com. Advantages of the Genaxxon ProbeMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Probe qPCR master mix high ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP 500nM ROX optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: Applied Biosystems® 5700, 7000, 7300, 7700, 7900, 7900 HT, Eppendorf Realplex4, StepOne™ and StepOnePlus™. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

GreenMasterMix FAST with 500nM ROX is optimised for realtime PCR assays in block systems. This 2X Mastermix is ready-to-use and contains all components for a successful and reliable qPCR with the exception of primer and template DNA. Advantages of the HotStart Taq DNA polymerase used in the Genaxxon GreenMasterMix: Hotstart technology enables setup of PCR mixture at room temperature. short initial denaturing time of not more than 2 minutes No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Green qPCR master mix with 500nM ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: Hotstart Taq DNA Polymerase dATP, dCTP, dGTP, dTTP intercalating green fluorescent dye optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) This Mastermix is specially suited for the following instruments: Applied Biosystems® 5700, 7000, 7300, 7700, 7900, 7900 HT, Eppendorf Realplex4, StepOne™ and StepOnePlus™. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

GreenMastermix High ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X PCR master mix is ready-to-use and contains optimised amounts of all ingredients. Advantages of the chemically modified SuperHot Taq polymerase used in the Genaxxon GreenMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Green qPCR master mix high ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP intercalating green fluorescent dye 500nM ROX optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: Applied Biosystems® 5700, 7000, 7300, 7700, 7900, 7900 HT, Eppendorf Realplex4, StepOne™ and StepOnePlus™. Other realtime PCR master mixes from Genaxxon are: M3023 - GreenMastermix No ROX >, M3045 - ProbeMastermix No ROX >, M3011 - GreenMastermix Low ROX >, M3031 - ProbeMastermix Low ROX >, M3052 - GreenMastermix High ROX >, M3010 - ProbeMastermix High ROX >.

ready-to-use PCR Mastermix with red Loading Dye for visual control of the pipetting steps and additional fluorescent dye for fast and easy detection of the DNA bands. After PCR, the PCR mix can be pipetted directly into the gel pockets without adding loading buffer. This makes our RedMasterMix Fluoro (2x) even more time- and cost-saving than our proven Red Mastermix. The RedMasterMix Fluoro (2x) with fluorescent gel staining dye and red loading dye also features high specificity for best results. The RedMasterMix Fluoro (2x) is a ready-to-use mixture of: - Taq DNA Polymerase- PCR reaction buffer- dNTPs- MgCl2- red loading dye- fluorescence gel staining dye for DNA band detection in an optimal concentration for efficient amplification of DNA templates by PCR. Only the primers and the template DNA have to be added. At the same time, the PCR Matermix contains an additive and a red dye, which allows subsequent electrophoresis without the addition of loading buffer. After electrophoresis, detection is performed directly under blue light without further staining. This saves additional time and costs. The RedMasterMix Fluoro (2x) with fluorescent dye was developed for use in routine PCR up to 4 kb amplicon length. The special composition of the buffer guarantees reproducible results even after repeated thawing and freezing cycles. Our Red MasterMix (2x) Fluoro is shipped in convenient aliquots of 1.25mL. For more information on our SimplyEnlight PCR products, read our blog now. Genaxxon's SimplyEnlight PCR - Unlock the Power of Your PCR!

RedMasterMix (2x) - PCR MasterMix with red dye to help visualize pipetting and mixing steps. Electrophoresis can be performed immediately after PCR without the need of a gel loading buffer. This makes this MasterMix time efficient, cost efficient and a reliable choice for the best PCR results with high efficiency.  One tube, one pipetting step. RedMasterMix (2x) is delivered to you as a optimized, ready-to-use solution containing- Taq DNA Polymerase - dNTPs- MgCl2- a red dye- reaction bufferfor efficient amplification of DNA. Additionally the RedMasterMix (2x) contains an additive and a red dye for proceeding with electrophoresis after PCR without adding loading buffer. Just add your primers and template DNA. This PCR MasterMix (2x) has been optimized for use in routine PCR amplification of DNA templates in the range of 0.2-4kb. The special formulation of our PCR RedMasterMix (2x) can withstand repeated freezing/thawing without compromising yields, sensitivity or results, when used as directed. The PCR MasterMix with red dye is efficient (no left over of reagents), scalable from 10µL to 50µL and stable for at least 24 months.Our RedMastermix can also be used for Sanger sequencing. Just dilute the PCR reaction 1:8 after PCR, or purify using spin columns and then apply. Read now in our blog how Genaxxon's RedMasterMix can also simplify your laboratory work. Our Agaroses > and DNA Ladders > are ideally suited for subsequent electrophoresis of PCR products.

The RedMastermix Hot (2x) from Genaxxon for fast and easy PCR setup and high reproducibility in standardized applications (Colony PCR, High-throughput PCR, Automated PCR applications). The Mastermix contains a red dye that allows direct application of the PCR product to the gel. The red dye enables monitoring of the gel run. Compared to standard Taq PCR mastermixes, the hotstart polymerase and the optimized buffer mixture with MgCl2 and dNTP results in an increased yield of amplicons up to 6kb even under Fast PCR conditions. Highly specific Hotstart Mastermix for routine applications. FAST PCR amplification up to 2kb. Ready-to-use, consisting of: Antibody inhibited Taq DNA Polymerase optimized PCR-Puffer dNTPs MgCl2 for your HotStart PCR. Ready-to-use Hotstart Mastermix. All you have to do is add the primers and the template DNA. The special composition of the buffer guarantees reproducible results even after repeated thawing and freezing cycles. The Hotstart Mastermix will be sent in 1mL aliquots. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and favourable standard agarose (M3044) > we can offer additional products for your PCR.

For reliable and easy PCR: PCR Master Mix for standard PCR applications (e.g. screenings) for amplifying DNA fragments <5kb. It is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by routine PCR (one tube, one pipetting step). Just add your primers and template DNA - that´s all.  The special formulation of our PCR Master Mix guarantees you reproducible results even after repeated freezing/thawing steps leading to good yields and sensitivity. The PCR Master Mix is efficient (no left over of reagents), scalable from 10µL to 50µL and stable for 24 months. Test sample available at a special price! The test sample price will be refunded on the first official order of the product.   Our Agaroses > and DNA Ladders > are ideally suited for subsequent electrophoresis of PCR products.

SNP Pol DNA polymerase for easy, reliable and rapid allele-specific discrimination, eg. CRISPR / Cas9 point mutations, for detecting incorrect CRISPR / Cas9 products, or validating sequencing results. The SNP Pol DNA polymerase distinguishes highly specific, whether a mismatch of the primer-template-complex is present or not. The mismatch (point mutation) must be at the 3 'end of the primer. Thus, mutant alleles can be distinguished exactly from wild-type alleles - without sequencing, since the polymerase simply does not amplify in the case of a mismatch. Just place your primer on the supposed point mutation (Important: The point mutation must be at the 3 'end) and the polymerase will detect a mismatch in this region with almost 100% accuracy: If the template base complementary to the 3' end of the primer shows the mutation and the primer does not, no amplification takes place - 100% certainty within a short time!The SNP Pol DNA polymerase can therefore be easily, time and cost-effectively used for the screening of point mutations. The variant SNP PolTaq DNA polymerase > has 5'-3 'nuclease activity and can therefore be used for specific hydrolysi probes such as Taqman® probes or Molecular beacons. For more information on our SNP DNA polymerases and applications, read our blog now. DescriptionSNP Pol DNA Polymerase is a highly selective DNA polymerase for the detection of Single Nucleotide Polymorphisms. It was developed specifically for allele-specific discrimination, where a very high discrimination rate is required e.g., in allele-specific PCR (ASA; AS-PCR), allele-specific primer extension (AS-PEX), SNP analysis, genotyping or in methylation-specific PCRs (MSP). Many other DNA polymerases tolerate mismatched primer-template complexes and are therefor not suitable. The SNP Pol DNA polymerase, on the other hand, distinguishes these specifically (high discrimination) and supplies only PCR products with perfectly matching primer pairs! The Genaxxon SNP Pol and SNP PolTaq DNA polymerases differ up to 100% by means of allele-specific PCR between the two alleles and, after a simple qPCR, give a clear result as to which allele is present. Thus, the principly great potential of the CRISPR/Cas9 technology can be used for human medicine and plant biotechnology especially together with the SNP PolTaq or SNP Pol DNA polymerase. Allele-specific PCR can be used to quantify the mutation rate in a pool or background of wild-type sequences. The verification of mutation frequencies determined by NGS can also be verified by means of allele-specific PCR and SNP Pol DNA polymerase. The SNP PolTaq DNA polymerase is very suitable for the analysis of liquid biopsy samples. With SNP PolTaq, the presence and frequency of cancer mutations can be analyzed and quantified very well. Picture below: Application note SNP Pol DNA Polymerase   We recommend short amplicon length (about 60-200 bp) for best results, but also longer amplicon lengths are possible. The addition of additional Magnesium (+0.5 - 1.5mM) might be needed in case of longer amplicons >500 bp.Trouble shooting: No bands after PCR of 30 cycles! Optimisation procedure for missing or weak bands.Annealing temperature is too low! Attention: The SNP Pol is an aptamer inhibited DNA polymerase. The aptamer oligo used reversibly inhibits the polymerase at temperatures <55°C! Therefore, the primers should ideally have an annealing temperature of >57°C. - realtime PCR approach - Check the dNTP concentration. This should be between 200 and 300µM (in the PCR). - Increase the number of cycles (at least 35, preferably equal to 40) Test optimisation - number of cyclesIn endpoint detection, a cycle count of 30 is not always sufficient to generate a clearly visible band. For example, with less than 200 DNA copies as a starting value. The test should therefore be run using real-time PCR, or five parallel PCRs should be used, one of which is taken from the PCR device after each of five different cycles (example below). Endpoint detection: Set up five identical PCR reactions in parallel using the SNP Pol DNA polymerase. Remove one of the PCR tubes from the cycler at each of 20, 25, 30, 35 and 40 cycles and apply all five reactions to an agarose gel at the end. This makes it easy to recognise which is the optimum number of cycles in the PCR for the given application (starting material, target, primer). SNP Pol PCR with cell lysates Animal cells and E.coli can be used directly for PCR with the SNP Pol DNA polymerase! No separate lysis or proteinase K digestion is necessary. PCR procedure:1. 50 - 500 cells are required per PCR batch! 2. prepare PCR mix with primers and buffer and place on ice! 3. add the picked colony directly to 10-20µL water (no separate lysis and no proteinase K digestion), vortex briefly (5 seconds), then add this cell suspension directly to the PCR reaction mixture, e.g. 10µL cell suspension for a PCR preparation with a total volume of 20µL. An initial denaturation time of 2-3 minutes is sufficient is sufficient, can be extended to 5 minutes if necessary. Genomic DNA per reaction max. 100 copies is relatively low, but should still work. The remainder of this cell suspension can also be frozen away in order to carry out further tests. Optional: 4. if possible: do real-time PCR!The SNP Pol DNA polymerase (M3009 > or M3061 >) can be used together with non-specific fluorescent dyes (eg Genaxxon's Green DNA Dye > or SybrGreen®) in real-time PCR. When working with specific PCR probes, only the SNP PolTaq DNA polymerase can be used, since only these have a 5'-3 'exonuclease activity. With our high quality dNTPs as Set (M3015.4100) > or Mix (M3016.1010) > or our DNALadders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR. Application areas for SNP Pol DNA and SNP Pol DNA polymerase- Monitoring, verification and detection of point mutations- Identification of correct or wrong CRISPR/Cas9 products- Verification/validation of sequencing results- Quantification of mutations (e.g. NGS results)- SNP-detection by allele-specific amplification (ASA) / Allele-specific PCR- Methylation specific PCRs (MSP) after bisulfite treated DNA (CpG methylation sides)- HLA genotyping- micro sequencing- realtime PCR with hydrolysis probes- realtime multiplex PCRs - DamID-seq data in C. elegans. As an alternative to ChIP, DNA adenine methyltransferase identification by sequencing (DamID-seq) was recently shown to be able to characterize binding sites in single mammalian cells. Additionally, DamID can be achieved for cell-type specific analysis by expressing Dam fusion proteins under tissue specific promoters in a controlled manner. In this report, we present a user-friendly pipeline to analyse DamID-seq data in C. elegans. Sharma R, Ritler D, Meister P.Tools for DNA adenine methyltransferase identification analysis of nuclear organization during C. elegans development. Genesis. 2016 Feb 4. doi: 10.1002/dvg.22925. - Minisequencing SNP genotyping with SNPase DNA Polymerase can be carried out by the procedure described in: Lovmar L, Fredriksson M, Liljedahl U, Sigurdsson S, Syvänen AC. Quantitative evaluation by minisequencing and microarrays reveals accurate multiplexed SNP genotyping of whole genome amplified DNA. Nucleic Acids Res. 2003;31:e129. - Allel specific mismatch selectivity by the HiDi DNA polymerase Drum M, Kranaster R, Ewald C, Blasczyk R, Marx A (2014) Variants of a Thermus aquaticus DNA Polymerase with Increased Selectivity for Applications in Allele- and Methylation-Specific Amplification. PLoS ONE 9(5): e96640. doi:10.1371/journal.pone.0096640

SNP Pol DNA polymerase for easy, reliable and rapid allele-specific discrimination, eg. CRISPR / Cas9 point mutations, for detecting incorrect CRISPR / Cas9 products, or validating sequencing results. The SNP Pol DNA polymerase distinguishes highly specific, whether a mismatch of the primer template complex is present or not. The mismatch (point mutation) must be at the 3 'end of the primer. Thus, mutant alleles can be distinguished exactly from wild-type alleles - without sequencing since the polymerase simply does not amplify in the case of a mismatch. Just place your primer on the supposed point mutation (Important: The point mutation must be at the 3 'end) and the polymerase will detect a mismatch in this region with almost 100% accuracy: If the template base complementary to the 3' end of the primer shows the mutation and the primer does not, no amplification takes place - 100% certainty within a short time!The SNP Pol DNA polymerase can therefore be easily, time and cost-effectively used for the screening of point mutations. SNP PolTaq DNA polymerase shows also 5'-3'-nuclease activity and is therefore suitable for hydrolysis probe-based assays (Taqman®, molecular beacons, etc.). For more information on our SNP DNA polymerases and applications, read our blog now. DescriptionSNP PolTaq DNA Polymerase is a highly selective DNA polymerase for the detection of Single Nucleotide Polymorphisms. It was developed specifically for allele-specific discrimination, where a very high discrimination rate is required e.g., in allele-specific PCR (ASA; AS-PCR), allele-specific primer extension (AS-PEX), SNP analysis, genotyping or in methylation-specific PCRs (MSP). Many other DNA polymerases tolerate mismatched primer-template complexes and are therefor not suitable. The SNP Pol DNA polymerase, on the other hand, distinguishes these specifically (high discrimination) and supplies only PCR products with perfectly matching primer pairs! The Genaxxon SNP Pol and SNP PolTaq DNA polymerases differ up to 100% by means of allele-specific PCR between the two alleles and, after a simple qPCR, give a clear result as to which allele is present. Thus, the principle great potential of the CRISPR/Cas9 technology can be used for human medicine and plant biotechnology especially together with the SNP PolTaq or SNP Pol DNA polymerase. Allele-specific PCR can be used to quantify the mutation rate in a pool or background of wild-type sequences. The verification of mutation frequencies determined by NGS can also be verified by means of allele-specific PCR and SNP Pol DNA polymerase. The SNP PolTaq DNA polymerase is very suitable for the analysis of liquid biopsy samples. With SNP PolTaq , the presence and frequency of cancer mutations can be analyzed and quantified very well. Picture below: Application note SNP PolTaq DNA Polymerase   We recommend short amplicon length (about 60-200 bp) for best results, but also longer amplicon lengths are possible. The addition of additional Magnesium (+0.5 - 1.5mM) might be needed in case of longer amplicons >500 bp.Trouble shooting: No bands after PCR of 30 cycles! Optimisation procedure for missing or weak bands.Annealing temperature is too low! Attention: The SNP PolTaq is an aptamer inhibited DNA polymerase. The aptamer oligo used reversibly inhibits the polymerase at temperatures <55°C! Therefore, the primers should ideally have an annealing temperature of >57°C. - realtime PCR approach - Check the dNTP concentration. This should be between 200 and 300µM (in the PCR). - Increase the number of cycles (at least 35, preferably equal to 40) Test optimisation - number of cyclesIn endpoint detection, a cycle count of 30 is not always sufficient to generate a clearly visible band. For example, with less than 200 DNA copies as a starting value. The test should therefore be run using real-time PCR, or five parallel PCRs should be used, one of which is taken from the PCR device after each of five different cycles (example below). Endpoint detection: Set up five identical PCR reactions in parallel using the SNP Pol DNA polymerase. Remove one of the PCR tubes from the cycler at each of 20, 25, 30, 35 and 40 cycles and apply all five reactions to an agarose gel at the end. This makes it easy to recognise which is the optimum number of cycles in the PCR for the given application (starting material, target, primer). SNP Pol PCR with cell lysates Animal cells and E.coli can be used directly for PCR with the SNP Pol DNA polymerase! No separate lysis or proteinase K digestion is necessary. PCR procedure:1. 50 - 500 cells are required per PCR batch! 2. prepare PCR mix with primers and buffer and place on ice! 3. add the picked colony directly to 10-20µL water (no separate lysis and no proteinase K digestion), vortex briefly (5 seconds), then add this cell suspension directly to the PCR reaction mixture, e.g. 10µL cell suspension for a PCR preparation with a total volume of 20µL. An initial denaturation time of 2-3 minutes is sufficient is sufficient, can be extended to 5 minutes if necessary. Genomic DNA per reaction max. 100 copies is relatively low, but should still work. The remainder of this cell suspension can also be frozen away in order to carry out further tests. Optional: 4. if possible: do real-time PCR!The SNP Pol DNA polymerase (M3009 > or M3061 >) can be used together with non-specific fluorescent dyes (eg Genaxxon's Green DNA Dye > or SybrGreen®) in real-time PCR. When working with specific PCR probes, only the SNP PolTaq DNA polymerase can be used, since only these have a 5'-3 'exonuclease activity. With our high quality dNTPs as Set (M3015.4100) > or Mix (M3016.1010) > or our DNALadders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR. Application areas for SNP Pol DNA and SNP PolTaq DNA polymerase- Monitoring, verification and detection of point mutations- Identification of correct or wrong CRISPR/Cas9 products- Verification/validation of sequencing results- Quantification of mutations (e.g. NGS results)- SNP-detection by allele-specific amplification (ASA) / Allele-specific PCR- Methylation specific PCRs (MSP) after bisulfite treated DNA (CpG methylation sides)- HLA genotyping- micro sequencing- realtime PCR with hydrolysis probes- realtime multiplex PCRs - DamID-seq data in C. elegans. As an alternative to ChIP, DNA adenine methyltransferase identification by sequencing (DamID-seq) was recently shown to be able to characterize binding sites in single mammalian cells. Additionally, DamID can be achieved for cell-type specific analysis by expressing Dam fusion proteins under tissue specific promoters in a controlled manner. In this report, we present a user-friendly pipeline to analyse DamID-seq data in C. elegans. Sharma R, Ritler D, Meister P.Tools for DNA adenine methyltransferase identification analysis of nuclear organization during C. elegans development. Genesis. 2016 Feb 4. doi: 10.1002/dvg.22925. - Minisequencing SNP genotyping with SNPase DNA Polymerase can be carried out by the procedure described in: Lovmar L, Fredriksson M, Liljedahl U, Sigurdsson S, Syvänen AC.bQuantitative evaluation by minisequencing and microarrays reveals accurate multiplexed SNP genotyping of whole genome amplified DNA. Nucleic Acids Res. 2003;31:e129. - Allel specific mismatch selectivity by the HiDi DNA polymerase Drum M, Kranaster R, Ewald C, Blasczyk R, Marx A (2014) Variants of a Thermus aquaticus DNA Polymerase with Increased Selectivity for Applications in Allele- and Methylation-Specific Amplification. PLoS ONE 9(5): e96640. doi:10.1371/journal.pone.0096640

The SNP Pol DNA polymerase > used for the SNP Pol 2X Mastermix has been specially designed for easy, reliable and rapid allele-specific discrimination, eg. CRISPR / Cas9 point mutations, for detecting incorrect CRISPR / Cas9 products, or validating sequencing results. The SNP Pol DNA polymerase distinguishes highly specific, whether a mismatch of the primer-template-complex is present or not. The mismatch (point mutation) must be at the 3 'end of the primer. Thus, mutant alleles can be distinguished exactly from wild-type alleles - without sequencing, since the polymerase simply does not amplify in the case of a mismatch. Just place your primer on the supposed point mutation (Important: The point mutation must be at the 3 'end) and the polymerase will detect a mismatch in this region with almost 100% accuracy: If the template base complementary to the 3' end of the primer shows the mutation and the primer does not, no amplification takes place - 100% certainty within a short time!The SNP Pol DNA polymerase can therefore be easily, time and cost-effectively used for the screening of point mutations. The variant SNP PolTaq DNA polymerase has 5'-3 'nuclease activity and can therefore be used for specific hydrolysi probes such as Taqman® probes or Molecular beacons. For more information on our SNP DNA polymerases and applications, read our blog now. DescriptionSNP Pol DNA Polymerase and SNP PolTaq Polymerase are  highly selective DNA polymerase for the detection of Single Nucleotide Polymorphisms. It was developed specifically for allele-specific discrimination, where a very high discrimination rate is required e.g., in allele-specific PCR (ASA; AS-PCR), allele-specific primer extension (AS-PEX), SNP analysis, genotyping or in methylation-specific PCRs (MSP). Many other DNA polymerases tolerate mismatched primer-template complexes and are therefor not suitable. The SNP Pol DNA polymerase, on the other hand, distinguishes these specifically (high discrimination) and supplies only PCR products with perfectly matching primer pairs! The Genaxxon SNP Pol and SNP PolTaq DNA polymerases differ up to 100% by means of allele-specific PCR between the two alleles and, after a simple qPCR, give a clear result as to which allele is present. Thus, the principly great potential of the CRISPR/Cas9 technology can be used for human medicine and plant biotechnology especially together with the SNP PolTaq or SNP Pol DNA polymerase. Allele-specific PCR can be used to quantify the mutation rate in a pool or background of wild-type sequences. The verification of mutation frequencies determined by NGS can also be verified by means of allele-specific PCR and SNP Pol DNA polymerase. The SNP PolTaq DNA polymerase is very suitable for the analysis of liquid biopsy samples. With SNP PolTaq, the presence and frequency of cancer mutations can be analyzed and quantified very well. Picture below: Application note SNP Pol DNA Polymerase   We recommend short amplicon length (about 60-200 bp) for best results, but also longer amplicon lengths are possible. The addition of additional Magnesium (+0.5 - 1.5mM) might be needed in case of longer amplicons >500 bp.Trouble shooting: No bands after PCR of 30 cycles! Optimisation procedure for missing or weak bands.Annealing temperature is too low! Attention: The SNP Pol is an aptamer inhibited DNA polymerase. The aptamer oligo used reversibly inhibits the polymerase at temperatures <55°C! Therefore, the primers should ideally have an annealing temperature of >57°C. - realtime PCR approach - Check the dNTP concentration. This should be between 200 and 300µM (in the PCR). - Increase the number of cycles (at least 35, preferably equal to 40) Test optimisation - number of cyclesIn endpoint detection, a cycle count of 30 is not always sufficient to generate a clearly visible band. For example, with less than 200 DNA copies as a starting value. The test should therefore be run using real-time PCR, or five parallel PCRs should be used, one of which is taken from the PCR device after each of five different cycles (example below). Endpoint detection: Set up five identical PCR reactions in parallel using the SNP Pol DNA polymerase. Remove one of the PCR tubes from the cycler at each of 20, 25, 30, 35 and 40 cycles and apply all five reactions to an agarose gel at the end. This makes it easy to recognise which is the optimum number of cycles in the PCR for the given application (starting material, target, primer). SNP Pol PCR with cell lysates Animal cells and E.coli can be used directly for PCR with the SNP Pol DNA polymerase! No separate lysis or proteinase K digestion is necessary. PCR procedure:1. 50 - 500 cells are required per PCR batch! 2. prepare PCR mix with primers and buffer and place on ice! 3. add the picked colony directly to 10-20µL water (no separate lysis and no proteinase K digestion), vortex briefly (5 seconds), then add this cell suspension directly to the PCR reaction mixture, e.g. 10µL cell suspension for a PCR preparation with a total volume of 20µL. An initial denaturation time of 2-3 minutes is sufficient is sufficient, can be extended to 5 minutes if necessary. Genomic DNA per reaction max. 100 copies is relatively low, but should still work. The remainder of this cell suspension can also be frozen away in order to carry out further tests. Optional: 4. if possible: do real-time PCR!The SNP Pol DNA polymerase (M3009 > or M3061 >) can be used together with non-specific fluorescent dyes (eg Genaxxon's Green DNA Dye > or SybrGreen®) in real-time PCR. When working with specific PCR probes, only the SNP PolTaq DNA polymerase can be used, since only these have a 5'-3 'exonuclease activity. With our high quality dNTPs as Set (M3015.4100) > or Mix (M3016.1010) > or our DNALadders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR. Application areas for SNP Pol DNA and SNP Pol DNA polymerase- Monitoring, verification and detection of point mutations- Identification of correct or wrong CRISPR/Cas9 products- Verification/validation of sequencing results- Quantification of mutations (e.g. NGS results)- SNP-detection by allele-specific amplification (ASA) / Allele-specific PCR- Methylation specific PCRs (MSP) after bisulfite treated DNA (CpG methylation sides)- HLA genotyping- micro sequencing- realtime PCR with hydrolysis probes- realtime multiplex PCRs - DamID-seq data in C. elegans. As an alternative to ChIP, DNA adenine methyltransferase identification by sequencing (DamID-seq) was recently shown to be able to characterize binding sites in single mammalian cells. Additionally, DamID can be achieved for cell-type specific analysis by expressing Dam fusion proteins under tissue specific promoters in a controlled manner. In this report, we present a user-friendly pipeline to analyse DamID-seq data in C. elegans. Sharma R, Ritler D, Meister P.Tools for DNA adenine methyltransferase identification analysis of nuclear organization during C. elegans development. Genesis. 2016 Feb 4. doi: 10.1002/dvg.22925. - Minisequencing SNP genotyping with SNPase DNA Polymerase can be carried out by the procedure described in: Lovmar L, Fredriksson M, Liljedahl U, Sigurdsson S, Syvänen AC. Quantitative evaluation by minisequencing and microarrays reveals accurate multiplexed SNP genotyping of whole genome amplified DNA. Nucleic Acids Res. 2003;31:e129. - Allel specific mismatch selectivity by the HiDi DNA polymerase Drum M, Kranaster R, Ewald C, Blasczyk R, Marx A (2014) Variants of a Thermus aquaticus DNA Polymerase with Increased Selectivity for Applications in Allele- and Methylation-Specific Amplification. PLoS ONE 9(5): e96640. doi:10.1371/journal.pone.0096640

The SNP PolTaq DNA polymerase used for the SNP PolTaq 2X Master Mix has been specially designed for easy, reliable and rapid allele-specific discrimination, eg. CRISPR / Cas9 point mutations, for detecting incorrect CRISPR / Cas9 products, or validating sequencing results. The SNP PolTaq DNA polymerase distinguishes highly specific, whether a mismatch of the primer-template-complex is present or not. The mismatch (point mutation) must be at the 3 'end of the primer. Thus, mutant alleles can be distinguished exactly from wild-type alleles - without sequencing, since the polymerase simply does not amplify in the case of a mismatch. Just place your primer on the supposed point mutation (Important: The point mutation must be at the 3 'end) and the polymerase will detect a mismatch in this region with almost 100% accuracy: If the template base complementary to the 3' end of the primer shows the mutation and the primer does not, no amplification takes place - 100% certainty within a short time!The SNP PolTaq DNA polymerase can therefore be easily, time and cost-effectively used for the screening of point mutations. The SNP PolTaq DNA polymerase has 5'-3 'nuclease activity and can therefore be used for specific hydrolysi probes such as Taqman® probes or Molecular beacons. For more information on our SNP DNA polymerases and applications, read our blog now. DescriptionSNP PolTaq DNA polymerase is a highly selective DNA polymerase for the detection of Single Nucleotide Polymorphisms. It was developed specifically for allele-specific discrimination, where a very high discrimination rate is required e.g., in allele-specific PCR (ASA; AS-PCR), allele-specific primer extension (AS-PEX), SNP analysis, genotyping or in methylation-specific PCRs (MSP). Many other DNA polymerases tolerate mismatched primer-template complexes and are therefor not suitable. The SNP PolTaq DNA polymerase, on the other hand, distinguishes these specifically (high discrimination) and supplies only PCR products with perfectly matching primer pairs! The Genaxxon SNP Pol and SNP PolTaq DNA polymerases differ up to 100% by means of allele-specific PCR between the two alleles and, after a simple qPCR, give a clear result as to which allele is present. Thus, the principly great potential of the CRISPR/Cas9 technology can be used for human medicine and plant biotechnology especially together with the SNP PolTaq or SNP Pol DNA polymerase. Allele-specific PCR can be used to quantify the mutation rate in a pool or background of wild-type sequences. The verification of mutation frequencies determined by NGS can also be verified by means of allele-specific PCR and SNP Pol DNA polymerase. The SNP PolTaq DNA polymerase is very suitable for the analysis of liquid biopsy samples. With SNP PolTaq, the presence and frequency of cancer mutations can be analyzed and quantified very well. Picture below: Application note SNP Pol DNA Polymerase   We recommend short amplicon length (about 60-200 bp) for best results, but also longer amplicon lengths are possible. The addition of additional Magnesium (+0.5 - 1.5mM) might be needed in case of longer amplicons >500 bp.Trouble shooting: No bands after PCR of 30 cycles! Optimisation procedure for missing or weak bands.Annealing temperature is too low! Attention: The SNP PolTaq is an aptamer inhibited DNA polymerase. The aptamer oligo used reversibly inhibits the polymerase at temperatures <55°C! Therefore, the primers should ideally have an annealing temperature of >57°C. - realtime PCR approach - Check the dNTP concentration. This should be between 200 and 300µM (in the PCR). - Increase the number of cycles (at least 35, preferably equal to 40) Test optimisation - number of cyclesIn endpoint detection, a cycle count of 30 is not always sufficient to generate a clearly visible band. For example, with less than 200 DNA copies as a starting value. The test should therefore be run using real-time PCR, or five parallel PCRs should be used, one of which is taken from the PCR device after each of five different cycles (example below). Endpoint detection: Set up five identical PCR reactions in parallel using the SNP Pol DNA polymerase. Remove one of the PCR tubes from the cycler at each of 20, 25, 30, 35 and 40 cycles and apply all five reactions to an agarose gel at the end. This makes it easy to recognise which is the optimum number of cycles in the PCR for the given application (starting material, target, primer). SNP Pol PCR with cell lysates Animal cells and E.coli can be used directly for PCR with the SNP Pol DNA polymerase! No separate lysis or proteinase K digestion is necessary. PCR procedure:1. 50 - 500 cells are required per PCR batch! 2. prepare PCR mix with primers and buffer and place on ice! 3. add the picked colony directly to 10-20µL water (no separate lysis and no proteinase K digestion), vortex briefly (5 seconds), then add this cell suspension directly to the PCR reaction mixture, e.g. 10µL cell suspension for a PCR preparation with a total volume of 20µL. An initial denaturation time of 2-3 minutes is sufficient is sufficient, can be extended to 5 minutes if necessary. Genomic DNA per reaction max. 100 copies is relatively low, but should still work. The remainder of this cell suspension can also be frozen away in order to carry out further tests. Optional: 4. if possible: do real-time PCR!The SNP Pol DNA polymerase (M3009 > or M3061 >) can be used together with non-specific fluorescent dyes (eg Genaxxon's Green DNA Dye > or SybrGreen®) in real-time PCR. When working with specific PCR probes, only the SNP PolTaq DNA polymerase can be used, since only these have a 5'-3 'exonuclease activity. With our high quality dNTPs as Set (M3015.4100) > or Mix (M3016.1010) > or our DNALadders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR. Application areas for SNP Pol DNA and SNP PolTaq DNA polymerase- Monitoring, verification and detection of point mutations- Identification of correct or wrong CRISPR/Cas9 products- Verification/validation of sequencing results- Quantification of mutations (e.g. NGS results)- SNP-detection by allele-specific amplification (ASA) / Allele-specific PCR- Methylation specific PCRs (MSP) after bisulfite treated DNA (CpG methylation sides)- HLA genotyping- micro sequencing- realtime PCR with hydrolysis probes- realtime multiplex PCRs - DamID-seq data in C. elegans. As an alternative to ChIP, DNA adenine methyltransferase identification by sequencing (DamID-seq) was recently shown to be able to characterize binding sites in single mammalian cells. Additionally, DamID can be achieved for cell-type specific analysis by expressing Dam fusion proteins under tissue specific promoters in a controlled manner. In this report, we present a user-friendly pipeline to analyse DamID-seq data in C. elegans. Sharma R, Ritler D, Meister P.Tools for DNA adenine methyltransferase identification analysis of nuclear organization during C. elegans development. Genesis. 2016 Feb 4. doi: 10.1002/dvg.22925. - Minisequencing SNP genotyping with SNPase DNA Polymerase can be carried out by the procedure described in: Lovmar L, Fredriksson M, Liljedahl U, Sigurdsson S, Syvänen AC. Quantitative evaluation by minisequencing and microarrays reveals accurate multiplexed SNP genotyping of whole genome amplified DNA. Nucleic Acids Res. 2003;31:e129. - Allel specific mismatch selectivity by the HiDi DNA polymerase Drum M, Kranaster R, Ewald C, Blasczyk R, Marx A (2014) Variants of a Thermus aquaticus DNA Polymerase with Increased Selectivity for Applications in Allele- and Methylation-Specific Amplification. PLoS ONE 9(5): e96640. doi:10.1371/journal.pone.0096640

Genaxxon's FAST DNA polymerase is a robust enzyme, ideally suited for applications like genotyping and screening, amplifying with extreme speed of up to 2 seconds per 1kb, as well as high yield and consistency. FAST DNA polymerase has 5’-3’ exonuclease activity, but no 3’-5’ exonuclease (proofreading) activity. PCR products generated with this enzyme are A-tailed and can thus be cloned into TA cloning vectors. The extreme speed of Genaxxons' FAST DNA polymerase allows the use of an extension rate of 4-8 kb/min. Genaxxon's FAST DNA Polymerase is delivered with 5X reaction buffer already containing MgCL2, dNTPs, enhancers and stabilizers for an optimal PCR result. Genaxxon's FAST DNA polymerase is also available as a convenient 2x master mix (M3286), including an inert red tracking dye for electrophoresis, containing all required components for fast PCR, except specific primers. Example of a typical PCR protocol (graphical presentation)* Ta is the annealing temperature (Ta should be 2°C above Tm**)  ** Tm is the melting temperature of a primer which is defined as the temperature at which 50% of the primer bind to the complementary sequence of the target DNA. Please note: By increasing Ta above Tm, this percentage decreases, however, primers will still anneal up to a certain degree and initiate extension. Therefore, PCR would still work with a Ta of several degrees higher than the Tm but with a dramatically reduced efficiency. Hence, we recommend optimizing the Ta by performing a temperature gradient (e.g., starting at the lowest Tm or a few degrees below and increasing with 2°C increments). *** Largest tested amplicon size: 5kb. Please note that longer extension times may be required for targets larger than 5kb!

Genaxxon’s FAST DNA polymerase (2X) MasterMix with dye is the master mix of our FAST DNA polymerase (M3285) which is a robust enzyme, ideally suited for applications like genotyping and screening, amplifying with extreme speed of up to 2 seconds per 1kb, as well as high yield and consistency. FAST DNA polymerase has 5’-3’ exonuclease activity, but no 3’-5’ exonuclease (proofreading) activity. PCR products generated with this enzyme are A-tailed and can thus be cloned into TA cloning vectors. The extreme speed of Genaxxon’s FAST DNA polymerase allows the use of an extension rate of 4-8 kb/min. Genaxxon bioscience’s FAST DNA Polymerase (2X) MasterMix with dye includes all components needed for a fast PCR, except the primers. Besides this, it includes an inert red tracking dye for gel electrophoresis. Therefore, upon completion of the PCR, the reaction is ready for direct loading onto an agarose gel without the need of adding an additional loading buffer.Example of a typical PCR protocol (graphical presentation)* Ta is the annealing temperature (Ta should be 2°C above Tm**)  ** Tm is the melting temperature of a primer which is defined as the temperature at which 50% of the primer bind to the complementary sequence of the target DNA. Please note: By increasing Ta above Tm, this percentage decreases, however, primers will still anneal up to a certain degree and initiate extension. Therefore, PCR would still work with a Ta of several degrees higher than the Tm but with a dramatically reduced efficiency. Hence, we recommend optimizing the Ta by performing a temperature gradient (e.g., starting at the lowest Tm or a few degrees below and increasing with 2°C increments). *** Largest tested amplicon size: 5kb. Please note that longer extension times may be required for targets larger than 5kb!

Genaxxon’s FAST HotStart DNA polymerase is a robust enzyme with an antibody mediated hotstart function, ideally suited for applications like genotyping and screening, amplifying with extreme speed of up to 2 seconds per 1kb, as well as high yield and consistency. PCR products generated with this enzyme are A-tailed and can thus be cloned into TA cloning vectors. The extreme speed of Genaxxon’s FAST HotStart DNA polymerase allows the use of an extension rate of 4-8 kb/min. The buffer composition has been optimized via high-throughput screening and makes the enzyme particularly resistant to PCR inhibitors. Therefore, Genaxxon’s FAST HotStart DNA polymerase is suitable for direct PCR from unpurified samples, including blood, urine, and bacterial colonies, making this the ideal choice for consistent results in fast complex PCR amplifications. Genaxxon's FAST HotStart DNA polymerase is also available as a convenient 2x master mix (M3288), including an inert red tracking dye for electrophoresis, containing all required components for fast PCR, except specific primers. Example of a typical PCR protocol (graphical presentation)* Ta is the annealing temperature (Ta should be 2°C above Tm**)  ** Tm is the melting temperature of a primer which is defined as the temperature at which 50% of the primer bind to the complementary sequence of the target DNA. Please note: By increasing Ta above Tm, this percentage decreases, however, primers will still anneal up to a certain degree and initiate extension. Therefore, PCR would still work with a Ta of several degrees higher than the Tm but with a dramatically reduced efficiency. Hence, we recommend optimizing the Ta by performing a temperature gradient (e.g., starting at the lowest Tm or a few degrees below and increasing with 2°C increments). *** Largest tested amplicon size: 5kb. Please note that longer extension times may be required for targets larger than 5kb!

Genaxxon’s FAST HotStart DNA polymerase (2X) MasterMix with dye is the master mix of our FAST HotStart DNA polymerase (M3287) which is a robust enzyme, ideally suited for applications like genotyping and screening, amplifying with extreme speed of up to 2 seconds per 1kb, as well as high yield and consistency.PCR products generated with this enzyme are A-tailed and can thus be cloned into TA cloning vectors. The extreme speed of Genaxxon’s FAST HotStart DNA polymerase allows the use of an extension rate of 4-8 kb/min. The buffer composition has been optimized via high-throughput screening and makes the enzyme particularly resistant to PCR inhibitors. Due to its robustness, Genaxxon’s FAST HotStart DNA polymerase (2X) MasterMix with dye is suitable for direct PCR from unpurified samples, including blood, urine, and bacterial colonies, making it the ideal choice for consistent results in fast complex PCR amplifications. Besides this, it includes an inert red tracking dye for gel electrophoresis. Therefore, upon completion of the PCR, the reaction is ready for direct loading onto an agarose gel without the need of adding an additional loading buffer.Example of a typical PCR protocol (graphical presentation)* Ta is the annealing temperature (Ta should be 2°C above Tm**)  ** Tm is the melting temperature of a primer which is defined as the temperature at which 50% of the primer bind to the complementary sequence of the target DNA. Please note: By increasing Ta above Tm, this percentage decreases, however, primers will still anneal up to a certain degree and initiate extension. Therefore, PCR would still work with a Ta of several degrees higher than the Tm but with a dramatically reduced efficiency. Hence, we recommend optimizing the Ta by performing a temperature gradient (e.g., starting at the lowest Tm or a few degrees below and increasing with 2°C increments). *** Largest tested amplicon size: 5kb. Please note that longer extension times may be required for targets larger than 5kb!

High-quality Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience Taq Polymerase allows amplification of DNA fragment of up to >7 kb. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer and separate MgCL2.The enzyme is delivered with our buffer component 'Buffer-S'. The buffer is optimised for high specificity amplification of DNA-templates. Our complete buffer contains 15mM MgCl2. Free Taq DNA Polymerase test sample available!No shipping costs within Germany. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.

High-quality Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience Taq Polymerase allows amplification of DNA fragment of >7 kb. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer and separate MgCL2.The enzyme is delivered with our buffer component 'Buffer-E'. The buffer is optimised for high yield amplification of DNA-templates. Our complete buffer contains 25mM MgCl2. Taq DNA Polymerase test sample available! No shipping costs within Germany. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.

Especially suitable for use in microbiology, the DNA free Taq Polymerase DF Taq S for high specificity is virtually free of foreign DNA. The special purification procedure guarantees DF Taq Polymerase free of any DNA impurities, especially free of DNA from the conservative region of the 16S ribosomal gene. DNA free DF Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience DNA free Taq Polymerase allows amplification of DNA fragments >7 kb. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer S and separate MgCl2. The buffer is optimised for high specificity amplification of DNA-templates. Our complete buffer S contains 15mM MgCl2. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.

Especially suitable for use in microbiology, the DNA freeTaq Polymerase DF Taq E for high yields is virtually free of foreign DNA. The special purification procedure guarantees Taq Polymerase free of any DNA impurities, especially free of DNA from the conservative region of the 16S ribosomal gene. DNA free DF Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience Taq Polymerase allows amplification of DNA fragments >7 kb. Test sample available at a special price! No shipment costs within Germany. The test sample price will be refunded on the first official order of the product. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer E and separate MgCL2. The buffer is optimised for high yield amplification of DNA-templates. Our complete buffer E contains ammonium sulfate and 30mM MgCl2. The special purification procedure guarantees Taq-Polymerase free of any DNA impurities, especially free of DNA from the conservative region of the 16S ribosomal gene. The special purification procedure guarantees Taq-Polymerase free of any DNA impurities, especially free of DNA from the conservative region of the 16S ribosomal gene. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.

Ampliqon Taq DNA Polymerase is developed for automation and freeze drying. It is a glycerol free formulation of standard Ampliqon Taq DNA Polymerase and is well suited for automated routine PCR applications, or where accurate pipetting of small amounts is crucial. Features:- Glycerol free storage buffer- Suitable for automated high throughput testing- Suitable for robot-aided pipetting- Suitable for applications involving freeze drying- High product yield- Processes up to 5 kb- dUTP incorporation possible Description:Ampliqon Taq DNA Polymerase Glycerol Free exhibits both a 5'→3'dA DNA polymerase and a 5'→3' exonuclease activity. The 5'-3' exonuclease activity leaves a 3' overhang on the PCR product, which are convenient for direct T-A cloning. Taq DNA Polymerase Glycerol Free lacks 3'-5' exonuclease activity and proofreading ability. Why glycerol?Glycerol is normally a major part of the storage buffer for enzymes, and acts as a cryoprotectant. Glycerol disrupts the water structure and makes the buffer more cell like, thus stabilising the polymerase. Glycerol is a highly viscous liquid and is therefore difficult and time-consuming to pipet accurately, especially in smaller volumes. As a consequence, pipetting glycerol in fast robot-aided automation processes is almost an unsolvable challenge. Furthermore, the presence of glycerol in the enzyme buffer makes freeze drying impossible. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.  

AQ97 High Fidelity DNA Polymerase ist eine proofreading Polymerase mit hoher Amplifikationsgeschwindigkeit und sehr hoher Genauigkeit (>60-fach) gegenüber Taq DNA Polymerase. AQ97 ist ideal für schwierige Targets mit sehr niedrigem oder hohem GC-Gehalt geeignet. Neben diesen Eigenschaften zeichnet sich die AQ97 auch durch Ihre Eignung, lange Targets bis zu 18 kb, zu amplifizieren aus. Dies führt zu sehr genauen und zuverlässigen PCR-Ergebnissen.  AQ97 High Fidelity DNA Polymerase besitzt sowohl eine 5'→3' DNA Polymerase-Aktivität als auch eine 3'→5' proofreading Exonuklease-Aktivität, die es dieser Polymerase ermöglicht, Basenpaar-Fehlpaarungen zu korrigieren. AQ97 High Fidelity DNA Polymerase ist ein fusionierter Proteinkomplex aus DNA-Polymerase mit einer prozessivitätssteigernden DNA-Bindungsdomäne. Neben einer sehr schnellen und robusten Amplifikation komplexer und langer Zielmoleküle zeichnet sich die AQ97 High Fidelity DNA Polymerase durch eine hohe Genauigkeit aus, was dazu führt, dass auch für lange Amplicons eine genaue Amplifikation gewährleistet ist. Dadurch eignet sich die AQ97 besonders für PCR-Experimente, die eine Amplifikation mit sehr niedrigen Fehlerraten erfordern, wie z. B. Klonen/Subklonen, NGS-Anwendungen, SNP-Analysen und Mutagenese. Eigenschaften: • High Fidelity: >60x Taq fidelity• High elongation rate: 10 sec/kb (bis 6000bp pro Minute)• Long range amplification: 18 kb for human gDNA• 5'-3' polymerase acitivity and 3'-5' exonuclease activity.• generates blund ends.Picture 1: Comparison figures of fidelity values for AQ97 DNA Polymerase, AccuPol DNA Polymerase, two well-recognized high fidelity DNA polymerases P and Q and Taq DNA Polymerase were determined through NGS-based analysis of nucleotide misincorporation during PCR. Initially, PCR amplification was performed on a ~ 200 bp synthetic DNA target, generating PCR products for each of the tested polymerases (using recommended setup conditions).  Picture 2: Distribution of substitution errors. PCR was performed using Taq DNA Polymerase, AQ97 High Fidelity DNA Polymerase, high fidelity DNA Polymerase Q and high fidelity DNA Polymerase P. The PCR was followed by NGS sequencing of the PCR products. The number of substitutions at each PCR target position was calculated and plotted in diagram A. Substitutions include misincorporated nucleotides and deletions at each position. Non-polymerase errors are subtracted from the total number of errors to revel true polymerase errors. Non-polymerase errors include mutations caused by thermocycling-induced DNA damage, pre-NGS sample preparation and sequencing errors. In these diagrams the average number of substitutions for Taq DNA Polymerase (Taq average) and for AQ97 High Fidelity DNA Polymerase (AQ97 average) is also plotted. Diagram B magnifies the area near the detection limit, displaying more information about the number of substitutions for AQ97 High Fidelity DNA Polymerase, high fidelity DNA Polymerase Q and high fidelity DNA polymerase P. Click to enlarge diagrams.  

AQ97 High Fidelity DNA Polymerase ist eine proofreading Polymerase mit hoher Amplifikationsgeschwindigkeit und sehr hoher Genauigkeit (>60-fach) gegenüber Taq DNA Polymerase. AQ97 ist ideal für schwierige Targets mit sehr niedrigem oder hohem GC-Gehalt geeignet. Neben diesen Eigenschaften zeichnet sich die AQ97 auch durch Ihre Eignung, lange Targets bis zu 18 kb, zu amplifizieren aus. Dies führt zu sehr genauen und zuverlässigen PCR-Ergebnissen.  AQ97 High Fidelity DNA Polymerase besitzt sowohl eine 5'→3' DNA Polymerase-Aktivität als auch eine 3'→5' proofreading Exonuklease-Aktivität, die es dieser Polymerase ermöglicht, Basenpaar-Fehlpaarungen zu korrigieren. AQ97 High Fidelity DNA Polymerase ist ein fusionierter Proteinkomplex aus DNA-Polymerase mit einer prozessivitätssteigernden DNA-Bindungsdomäne. Neben einer sehr schnellen und robusten Amplifikation komplexer und langer Zielmoleküle zeichnet sich die AQ97 High Fidelity DNA Polymerase durch eine hohe Genauigkeit aus, was dazu führt, dass auch für lange Amplicons eine genaue Amplifikation gewährleistet ist. Dadurch eignet sich die AQ97 besonders für PCR-Experimente, die eine Amplifikation mit sehr niedrigen Fehlerraten erfordern, wie z. B. Klonen/Subklonen, NGS-Anwendungen, SNP-Analysen und Mutagenese. Eigenschaften: • High Fidelity: >60x Taq fidelity• High elongation rate: 10 sec/kb (bis 6000bp pro Minute)• Long range amplification: 18 kb for human gDNA• 5'-3' polymerase acitivity and 3'-5' exonuclease activity.• generates blund ends.Picture 1: Comparison figures of fidelity values for AQ97 DNA Polymerase, AccuPol DNA Polymerase, two well-recognized high fidelity DNA polymerases P and Q and Taq DNA Polymerase were determined through NGS-based analysis of nucleotide misincorporation during PCR. Initially, PCR amplification was performed on a ~ 200 bp synthetic DNA target, generating PCR products for each of the tested polymerases (using recommended setup conditions).  Picture 2: Distribution of substitution errors. PCR was performed using Taq DNA Polymerase, AQ97 High Fidelity DNA Polymerase, high fidelity DNA Polymerase Q and high fidelity DNA Polymerase P. The PCR was followed by NGS sequencing of the PCR products. The number of substitutions at each PCR target position was calculated and plotted in diagram A. Substitutions include misincorporated nucleotides and deletions at each position. Non-polymerase errors are subtracted from the total number of errors to revel true polymerase errors. Non-polymerase errors include mutations caused by thermocycling-induced DNA damage, pre-NGS sample preparation and sequencing errors. In these diagrams the average number of substitutions for Taq DNA Polymerase (Taq average) and for AQ97 High Fidelity DNA Polymerase (AQ97 average) is also plotted. Diagram B magnifies the area near the detection limit, displaying more information about the number of substitutions for AQ97 High Fidelity DNA Polymerase, high fidelity DNA Polymerase Q and high fidelity DNA polymerase P. Click to enlarge diagrams.  

The High-Fidelity Pfu proofreading DNA polymerase from Genaxxon bioscience is a thermostable, highly processive enzyme possessing 5'-3' DNA polymerase with additional 3'-5' proofreading exonuclease activity, which enables the correction of nucleotide incorporation errors. It has no 5'→3' exonuclease activity. The High-Fidelity Pfu DNA polymerase is a recombinant form of the hyperthermophilic archaebacteria Pyrococcus furiosus (Pfu). Pfu proofreading DNA polymerase shows an increased thermostability and a 10-times higher accuracy compared to Taq DNA polymerase. A mixture of Taq DNA Polymerase and Pfu DNA polymerase provides more robust synthesis of longer amplification products (Barnes, 1994. Proc. Natl. Acad. Sci. USA 91:2216-2220). Test sample available at a special price! The test sample price will be refunded on the first official order of the product. Features: 10-times higher accuracy compared to Taq DNA polymerase High-Fidelity polymerase Proofreading function (3' - 5' exonuclease activity) High thermo stability Generates blunt-end PCR products Generates PCR products for cloning and expression More High-Fidelity Proofreading Polymerases from Genaxxon bioscience:- M3003 ReproFast Proofreading Polymerase- M3002 Pwo Proofreading Polymerase- M3012 ReproHot (KOD) Proofreading Polymerase - AQ97 High Fidelity proofreading Polymerase With our high quality dNTPs as Set (M3015) or Mix (M3016) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.

The High-Fidelity Pwo proofreading DNA polymerase from Genaxxon bioscience is a thermostable, highly processive enzyme possessing 5'-3' DNA polymerase with additional 3'-5' proofreading exonuclease activity, which enables the correction of nucleotide incorporation errors. It has no 5'→3' exonuclease activity. The High-Fidelity Pwo DNA polymerase is a recombinant form of the hyperthermophilic archaebacteria Pyrococcus woesei. Pwo proofreading DNA polymerase shows an increased thermostability and a 10-times higher accuracy compared to Taq DNA polymerase. A mixture of Taq DNA Polymerase and Pwo DNA polymerase provides more robust synthesis of longer amplification products (Barnes, 1994. Proc. Natl. Acad. Sci. USA 91:2216-2220). Test sample available at a special price! The test sample price will be refunded on the first official order of the product. Features: 10-times higher accuracy compared to Taq DNA polymerase High-Fidelity polymerase Proofreading function (3' - 5' exonuclease activity) High thermo stability Generates blunt-end PCR products Generates PCR products for cloning and expression More High-Fidelity Proofreading Polymerases from Genaxxon bioscience:- M3003 ReproFast Proofreading Polymerase- M3004 Pfu Proofreading Polymerase- M3012 ReproHot (KOD) Proofreading Polymerase - AQ97 High Fidelity proofreading Polymerase With our high quality dNTPs as Set (M3015) or Mix (M3016) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.

ReproFast high-fidelity DNA polymerase provides high overall performance and more robust amplification of longer targets due to Genaxxons polymerase-enhancing factor. The use of ReproFast polymerase enhances overall PCR performance, including shorter extension times, higher yield and greater target length capability compared to that from standard Pfu- or Pwo DNA polymerase. The main application of our ReproFast is for amplification of long target sequences, including genomic DNA giving high accuracy for cloning experiments. With ReproFast it will be possible to amplify fragments up to 5 kb from human genomic DNA and up to 7 kb from lambda DNA (Barnes (1994) Proc. Natl. Acad. Sci. USA 91. 2216-2220) with the accuracy of Pfu- or Pwo polymerases. The Genaxxon bioscience ReproFast DNA-Polymerase is a thermostable enzyme possessing 5‘-3‘ DNA polymerase and 3‘-5‘ proofreading exonuclease activities (generates no A-overhangs / suitable for blund-end cloning). Test sample available at a special price! The test sample price will be refunded on the first official order of the product. More High-Fidelity Proofreading Polymerases from Genaxxon bioscience: - M3002 Pwo Proofreading Polymerase- M3004 Pfu Proofreading Polymerase- M3012 ReproHot (KOD) Proofreading Polymerase- AQ97 High Fidelity proofreading Polymerase With our high quality dNTPs as Set (M3015) or Mix (M3016) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.

ReproHot is a high fidelity DNA polymerase for accurate PCR amplification of GC-rich templates used for cloning and cDNA amplification. This enzyme is a mixture of Taq DNA polymerase plus a proofreading polymerase and a monoclonal antibody, which blocks polymerase activity prior to heating. The complex formed between the polymerase and the antibody prevents primer-dimer and other artifacts resulting from unspecific primer binding. The antibody is inactivated while heating up above 75°C, requiring no prolonged first heating step prior to cycling. The combined hotstart and proofreading feature leads to highly specific and correct amplicons. ReproHot can be used as a cheap alternative to Phusion DNA polymerase from. NEB or other sources. More High-Fidelity Proofreading Polymerases from Genaxxon bioscience: - M3002 Pwo Proofreading Polymerase- M3003 ReproFast Proofreading Polymerase- M3004 Pfu Proofreading Polymerase With our high quality dNTPs as Set (M3015) or Mix (M3016) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.

Taq DNA Hot Start Polymerase with Aptamer technology. The specific aptamer inhibits DNA polymerase activity below temperatures of 50°C to 55°C, thus enabling hot start PCR. At higher temperatures, the aptamer falls off the active site of Taq polymerase. The Hot Start Taq Polymerase can be used like a normal Taq without having to lengthen the first denaturation step.The binding of aptamer and polymerase prevents the formation of primer-dimer constructs and other artefacts revealed by non-specific primer binding. Check also our SuperHot Mastermix with or without dye. With our high quality dNTPs as Set or Mix or our DNA Ladders and our favourable standard agarose we can offer additional products for your PCR.

SuperHot Taq DNA Polymerase is a superior DNA Polymerase for Real Time PCR, Hot-Start PCR, low-copy number PCR, or PCR of difficult templates. The enzyme is developed to enhance the specificity, sensitivity and yield of DNA amplification. SuperHot Taq DNA polymerase is a chemicaly modified form of thermostable DNA polymerase Taq, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and before the first PCR cycle, the enzyme is not active and misprimed primers are not extended. As a result specifity and efficiency are increased by far compared to standard Taq DNA polymerase. Additionally, difficult targets with high GC-content can be amplified. For realtime PCR Genaxxon offers specially optimized 2-times qPCR mastermix >, respective a special Multiplex-PCR mastermix >. Features of Genaxxons SuperHot DNA Polymerase chemically modified increased specifity increased sensitivity for difficult templates with high GC-content Please have also a look on our SuperHot Taq 2-times Mastermix >. Even more convenient than the SuperHot Taq. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and favourable standard agarose (M3044) > we can offer additional products for your PCR.

Highly specific Hotstart master mix for routine and diagnostic PCR and qPCR up to 4kb: ready-to-use master mix with chemical modified Taq DNA polymerase. This Hotstart Master Mix for qPCR is a premixed, ready-to-use solution containing chemically modified Taq DNA Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR and qPCR (one tube, one pipetting step). Just add your primers and template DNA. The Hotstart PCR Master Mix has been optimized for use in routine and diagnostic Hotstart PCR and qPCR for amplifying DNA template in the range of 0.2-4kb. The special formulation of our Hotstart Master Mix guarantees you reproducible results even after repeated freezing/thawing steps leading to good yields and sensitivity. The Hotstart Master Mix is efficient (no left over of reagents), scalable from 10µL to 50µL and stable for 12 months. This Hotstart PCR master mix is also available as SuperHot Mastermix Blue > enabling visualisation of each pipetting step. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and favourable standard agarose (M3044) > we can offer additional products for your PCR.

Biotin-11-dUTP (Biotin-11-2'-deoxyuridin-5'-triphosphat - Tetralithiumsalt) is a commonly used component for non-radioactive labelling of DNA. Biotin-11-dUTP can be incorporated into DNA enzymatically by Nick-Translation, random-priming, 3'-end terminal labelling or by PCR. The figure '11' stands for the number of carbon atoms of the linker between dUTP and Biotin. The longer the 'linker-chain' the better the interaction between Biotin and Avidin. The shorter the linker the better the incorporation of dUTP into DNA. '11' has been shown to be a good compromise for most applications. The product Biotin-11-dUTP for non-radioactive labelling of DNA is delivered as 1 mM solution in 10 mM Tris-HCl (pH7.5), 1 mM EDTA. Biotin-11-dUTP can be enzymatically incorporated into DNA with Taq DNA Polymerase >, phi29 DNA Polymerase, Klenow Fragment, and DNA Polymerase I. Besides Biotin-11-dUTP Genaxxon offers other modified nucleotides or non modified dNTPs as our PCR dNTP-Mix> with 2 mM > or 10 mM > respective our dNTP-Set 100mM >. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001) >, or our proof-reading polymerases Pfu (M3004) >, Pwo (M3002) > and ReproFast (M3003) >, as well as the ready-to-use RedMastermix (M3029) >, already including dNTPs and a red dye.

Highly pure, HPLC purified (>99%) dNTPs packaged as 4 separate 100mM solutions of dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. Genaxxon’s dNTP solutions have been optimized for use in DNA amplification and other related methods. Genaxxon dNTPs contain no measurable bacterial or human DNA. For long term storage and/or for repeated use, our recommendation is to aliquot the stock solutions. Solutions of dNTP sodium salts (dATP, dCTP, dGTP und dTTP) Concentration: 100 mM each nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mixes with 2 mM or 10 mM or the modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003) as well as the ready-to-use RedMastermix (M3029), already including dNTPs.

Deoxynucleotide (dNTP) Solution Mix as an equimolar solution of ultrapure, HPLC purified (>99%) dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. The Genaxxon dNTP solutions are optimized for use in DNA amplification and other related methods. The Genaxxon dNTPs and dNTP-mixes contain no measurable bacterial or human DNA. For long term storage and/or for repeated use we do recommend to aliquot the stock solutions. Mixture of dNTP sodium salts (dATP, dCTP, dGTP und dTTP) Concentration: 10 mM each nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mix withv2 mM dNTP mix , our dNTP set, or modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003), as well as the ready-to-use RedMastermix (M3029), already including dNTPs.

Highly pure, HPLC purified dNTPs (>99%) delivered as a 2 mM solution of dATP, dCTP, dGTP and dTTP for use in qPCR, standard PCR, RT-PCR and Klenow reactions. Use 5 µL of Mix for PCR in 50µL reaction volume.The Genaxxon dNTP mix is optimized for its use in DNA polymerisation and related methods. Our dNTPs contain no measurable bacterial or human DNA. For storage for a prolonged periode of time we recommend to prepare small aliquots, especially in case of rare use. Mixture of dNTP sodium salt solutions (dATP, dCTP, dGTP und dTTP) Concentration: 2 mM of ach nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mix with 10 mM dNTP mix, our dNTP set , or modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003), as well as the ready-to-use RedMastermix (M3029), already including dNTPs.

Highly pure, HPLC purified 2'-Deoxyadenosine 5'-triphosphate (dATP >99%) delivered as 100 mM soution for use in qPCR, standard PCR, RT-PCR and Klenow reactions. The Genaxxon dNTP solutions are optimized for their use in DNA polymerisation and related methods. Our dNTPs contain no measurable bacterial or human DNA. For storage for a prolonged periode of time we recommend to prepare small aliquots, especially in case of rare use. Sodium salt solutions of the 4 nucleotides: dATP, dCTP, dGTP and dTTP Concentration: 100 mM of each nucleotide Genaxxon offers also PCR dNTP-Mix 10 mM >, unser dNTP-Set as 4 x 100 mM solution > or modified nucleotides as Biotin-11-dUTP >. For your successful PCR you will find different enzymes or PCR master mixes: Taq DNA Polymerse (M3001) >, Proof-Reading Polymerases Pfu (M3004) >, Pwo (M3002) > and ReproFast (M3003) >, as well as our  ready-to-use RedMastermix (M3029) >, containing dNTPs already.

100mM solution of 2'-Deoxycytidine 5'-triphosphate (dCTP) of purity >99%. Product has been tested for PCR products of length up to 10kb. Highly pure, HPLC purified Deoxycytidine 5'-triphosphate, tetrasodium salt (dCTP / >99%) delivered as 100 mM soution for use in qPCR, standard PCR, RT-PCR and Klenow reactions. The Genaxxon dNTP solutions are optimized for their use in DNA polymerisation and related methods. Our dNTPs contain no measurable bacterial or human DNA. For storage for a prolonged periode of time we recommend to prepare small aliquots, especially in case of rare use. Sodium salt solutions of the 4 nucleotides: dATP, dCTP, dGTP and dTTP Concentration: 100 mM of each nucleotide Genaxxon offers also PCR dNTP-Mix 10 mM >, unser dNTP-Set as 4 x 100 mM solution > or modified nucleotides as Biotin-11-dUTP >. For your successful PCR you will find different enzymes or PCR master mixes: Taq DNA Polymerse (M3001) >, Proof-Reading Polymerases Pfu (M3004) >, Pwo (M3002) > and ReproFast (M3003) >, as well as our  ready-to-use RedMastermix (M3029) >, containing dNTPs already.

Highly pure, HPLC purified 2'-Deoxyguanosine 5'-triphosphate (dGTP / >99%) delivered as 100 mM soution for use in qPCR, standard PCR, RT-PCR and Klenow reactions. The Genaxxon dNTP solutions are optimized for their use in DNA polymerisation and related methods. Our dNTPs contain no measurable bacterial or human DNA. For storage for a prolonged periode of time we recommend to prepare small aliquots, especially in case of rare use. Sodium salt solutions of the 4 nucleotides: dATP, dCTP, dGTP and dTTP Concentration: 100 mM of each nucleotide Genaxxon offers also PCR dNTP-Mix 10 mM >, unser dNTP-Set as 4 x 100 mM solution > or modified nucleotides as Biotin-11-dUTP >. For your successful PCR you will find different enzymes or PCR master mixes: Taq DNA Polymerse (M3001) >, Proof-Reading Polymerases Pfu (M3004) >, Pwo (M3002) > and ReproFast (M3003) >, as well as our  ready-to-use RedMastermix (M3029) >, containing dNTPs already.

Highly pure, HPLC purified 2'-Deoxythymidine 5'-triphosphate (dTTP / >99%) delivered as 100 mM soution for use in qPCR, standard PCR, RT-PCR and Klenow reactions. The Genaxxon dNTP solutions are optimized for their use in DNA polymerisation and related methods. Our dNTPs contain no measurable bacterial or human DNA. For storage for a prolonged periode of time we recommend to prepare small aliquots, especially in case of rare use. Sodium salt solutions of the 4 nucleotides: dATP, dCTP, dGTP and dTTP Concentration: 100 mM of each nucleotide Genaxxon offers also PCR dNTP-Mix 10 mM >, unser dNTP-Set as 4 x 100 mM solution > or modified nucleotides as Biotin-11-dUTP >. For your successful PCR you will find different enzymes or PCR master mixes: Taq DNA Polymerse (M3001) >, Proof-Reading Polymerases Pfu (M3004) >, Pwo (M3002) > and ReproFast (M3003) >, as well as our  ready-to-use RedMastermix (M3029) >, containing dNTPs already.

Deoxyuridine 5'-triphosphate, tetrasodium salt (dUTP) of purity >99% (HPLC). dUTP may be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. Product has been tested for PCR products of length up to 8kb (Taq DNA polymerase) and 0.8kb (Pfu DNA polymerase). The substitution of dTTP for dUTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme Uracil-DNA-Glycosylase, UDG >, can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives. Genaxxon offers also PCR dNTP-Mix 10 mM >, unser dNTP-Set as 4 x 100 mM solution > or modified nucleotides as Biotin-11-dUTP >. For your successful PCR you will find different enzymes or PCR master mixes: Taq DNA Polymerse (M3001) >, Proof-Reading Polymerases Pfu (M3004) >, Pwo (M3002) > and ReproFast (M3003) >, as well as our  ready-to-use RedMastermix (M3029) >, containing dNTPs already.

2',3'-Dideoxyadenosine-5'-O-triphosphate (ddATP) sodium salt of purity >95% HPLC. For other salt forms or a guaranteed higher purity please inquire. 2',3'-Dideoxyadenosine-5'-Triphosphate (ddATP) is a modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination while PCR. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is widely useful in antiviral research and in a variety of biotechnology applications. ddATP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations. Reference: Sanger et al., Proc. Natl. Acad. Sci. USA, 74, 5463 - 5467 (1977). ddATP is an analogue of cyditine triphosphate (ATP) where both ribose hydroxy groups have been removed and replaced by hydrogen.

2',3'-Dideoxycytidine-5'-O-triphosphate (ddCTP) sodium salt of purity >95% HPLC. For other salt forms or a guaranteed higher purity please inquire. 2',3'-Dideoxycytidine-5'-Triphosphate (ddCTP) is a modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination while PCR and is widely useful in antiviral research and in a variety of biotechnology applications. ddCTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations. Reference: Sanger et al., Proc. Natl. Acad. Sci. USA, 74, 5463 - 5467 (1977). ddCTP is an analogue of cyditine triphosphate (CTP) where both ribose hydroxy groups have been removed and replaced by hydrogen.

2',3'-Dideoxyguanosine-5'-O-triphosphate (ddGTP) sodium salt of purity >95% HPLC. For other salt forms or a guaranteed higher purity please inquire. 2',3'-Dideoxyguanosine-5'-triphosphate (ddGTP) is a modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination while PCR and is widely useful in antiviral research and in a variety of biotechnology applications. ddGTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations. Reference: Sanger et al., Proc. Natl. Acad. Sci. USA, 74, 5463 - 5467 (1977). ddGTP is an analogue of guanosine triphosphate (GTP) where both ribose hydroxy groups have been removed and replaced by hydrogen.

3'-Deoxythymidine-5'-O-triphosphate (2',3'-Dideoxythymidine-5'-O-triphosphate) (dTTP/ddTTP) sodium salt of purity >95% HPLC. For other salt forms or a guaranteed higher purity please inquire. Note: Since “thymidine“ already describes a 2'- deoxy nucleosides, the term “2'-deoxythymidine“ is redundant. 3'-Deoxythymidine-5'-O-triphosphate (dTTP/ddTTP) is a modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination while PCR and is widely useful in antiviral research and in a variety of biotechnology applications. dTTP/ddTTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations. Reference: Sanger et al., Proc. Natl. Acad. Sci. USA, 74, 5463 - 5467 (1977). dTTP/ddTTP is an analogue of thymidine triphosphate (TTP) where both ribose hydroxy groups have been removed and replaced by hydrogen. Amount and packaging:The product is delivered as 500µL of a10mM solution, pH7.6 (500µL correspond to 5µmol (appr. 2.3mg)).

The GenLadder 100 bp Plus with gel staining dye and loading dye - ready-to-use is an innovation for high throughput approaches or colony screenings! Ideally suited to determine the size of double-stranded DNA between 100 and 1000 base pairs. The additional bands at 1.5kbp and 3.0kbp allow additional DNA sizes in this range to be assigned without having to use an additional 1kbp DNA marker. At the same time, the mixture also contains a fluorescent gel staining dye. Additional staining (pre- or post staining) or the addition of fluorescent dye prior to application is thus unnecessary and saves an additional pipetting step. At the same time, it is possible to check whether a PCR was successful or not before applying the PCR sample! This DNA ladder is an ideal match for our Red MasterMix Fluoro 2X, which also contains the gel staining dye. Together the ideal pair for Colony Screens! For more information on our SimplyEnlight PCR products, read our blog now. Genaxxon's SimplyEnlight PCR - Unlock the Power of Your PCR! The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp. The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 3000 bp (40ng/6µL), 2x 1500 bp (70ng/6µL), 1000 bp (50ng/6µL), 900 bp (40ng/6µL), 800 bp (40ng/6µL), 700 bp (30ng/6µL), 600 bp (30ng/6µL), 2x 500 bp (90ng/6µL), 400 bp (40ng/6µL), 300 bp (30ng/6µL), 200 bp (40ng/6µL), 100 bp (40ng/6µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 90 µg/mL. The recommended amount of DNA marker per lane is 0,5μg (6µL). GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3084 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

Pure, quality tested, nuclease-free water is suitable for use in all experiments that require nuclease-free water, including molecular biology applications. Nuclease-free water is processed, to yield DNase, RNase, and nuclease-free, deionized water. Contamination of water with nucleases may lead to inconsitencies or even complete failures of experiments. For this Genaxxon offers different grades of nuclease free water: sterile, Diethylpyrocarbonate (DEPC) treated water (M6082 >) and nuclease free water (not DEPC treated) (M6340 >). Our Nuclease-Free Water is provided in nuclease-free PE bottles or in glass bottles.

Loading buffer I Fluoro is a fluorescent reagent that produces instant visualization of DNA bands upon blue light or UV illumination of agarose gels. Supplied in 6X DNA Loading Buffer, Loading buffer I Fluoro is used to prepare DNA markers or samples for loading on agarose or polyacrylamide gels.  Loading buffer I Fluoro is the sensitive staining reagent available for detecting the double-stranded DNA (dsDNA). It contains three tracking dyes (bromophenol blue, xylene cyanol FF, and orange G) for visually tracking the DNA migration during the electrophoresis plus an additional fluorescence dye for detection of DNA bands without an additional staining of the gel. It is an ideal alternative to Ethidium Bromide (EtBr). Approximate fluorescence excitation / emission: 300, 495 / 537 nm, bound to nucleic acid. For more information on our SimplyEnlight PCR products, read our blog now. Genaxxon's SimplyEnlight PCR - Unlock the Power of Your PCR! Picture 1: Comparison of UV- and blue light excitation             Picture 2: Effect of EtBr plus UV on cloning efficiency.

Gel Loading Dye I 6X is a loading buffer for DNA application on gels. Buffer contains Glycerol, EDTA, Bromophenol Blue and Xylene cyanol. Genaxxon 6X DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (bromophenol blue and xylene cyanol FF) for visual tracking of DNA migration during electrophoresis.

DNA 6X Loading buffer is a special loading buffer for DNA application on gels that enables viusalization of DNA bands smaller than 100 bp without problems. Buffer contains Glycerol, EDTA, Orange G. The special dye gives the opportunity to detect even DNA bands running below 65 bp. Other loading dye: M3308 and PCR dye: M3309.

Sterile distilled water free of DNAses, Rnases and Proteinases. Water treated with DEPC to ensure absence of Rnase. For that reason this water is especially suited for RT-PCR or other molecular biology application where 100% absence of RNAse is necessary (resuspension of DNA, dilution of enzymes, etc.). Genaxxon offers different grades of nuclease free water: sterile, Diethylpyrocarbonate (DEPC) treated water (M6082 >) and nuclease free water (not DEPC treated) (M6340 >).

Oligo (dT)20 are single stranded oligodeoxyribonucleotides containing only deoxythymine (dT) to be able to prime with the poly(A) tail of mRNA molecules. Primers are quality controlled (MS), purified (reversed phase HPLC), aliquoted and lyophilized and came with a guaranteed amount of at least 5OD (about 27.2nmol (163.7µg)). Oligo (dT)20 Primer is designed to initiate the synthesis of cDNA from total RNA in a reverse transcription reaction, where Reverse Transcriptase, e.g. MMuLV (M3042) > is starting the reaction from the poly-A-end of mRNA. It can also be used for generation of labeled cDNA to screen microarrays. A mixture (1:1 ratio) of random hexamer primers (M3038 >) and Oligo(dT)20 primer may improve the sensitivity of cDNA synthesis as it will especially improve the efficiency of transcripts longer than 600 bp. Guaranteed delivery of at least 5OD (equals about 27.2nmol (163.7µg)). Primers are quality controlled (MS), purified (reversed phase HPLC), lyophilised and aliquoted. Dissolve content (5 OD) in 271.5μL of molecular biology grade water for an end concentration of 100 pmol/μL (100μM). 760μL (100μM) are sufficient for 760 reverse transcriptions of 20μL each. Oligo (dT)20 Primer is suitable for use as a primer for first strand cDNA synthesis with a reverse transcriptase, such as MMuLV or AMV. The primer hybridizes to the poly-adenylated tail found on the 3´ end of most eukaryotic mRNAs. Oligo (dT)20 ensures that the 3´ end of mRNAs are represented. Oligo(dT)20 Primer may be better suited for the new generation of Reverse Transcriptases like SuperScript™ that work at higher temperatures as the longer Oligo(dT)20 Primer enable annealing in reverse transcription reactions at higher temperatures. Applications• cDNA synthesis from total RNA in a reverse transcription reaction.• optimal choice for construction of cDNA libraries from eukaryotic mRNAs.• Full length cDNA cloning• 3’ rapid amplification of cDNA ends (3’ RACE)• Generation of labelled cDNA for screening of microarrays.Oligo (dT)20 Primer can not be used together with degraded RNA, prokaryotic RNA or miRNA (lack of poly(A) tail). Primer Sequence: 5´ – d (TTT TTT TTT TTT TTT TTT TT) –3´

Random Hexamers are short oligodeoxyribonucleotides of random sequence [d(N)6]. Primers are quality controlled (MS), purified (reversed phase HPLC), lyophilised and aliquoted and came with a guaranteed amount of at least 5OD (about 76.1nmol (136.4µg)). Hexanucleotide primers are a mixture of random 5'-hydroxyl hexanucleotides or hexamers, and can be used to quickly and efficiently prepare radioactive or non-radioactive probes using a DNA polymerase and a suitable DNA template or for cDNA synthesis from mRNA. The heterogeneous nature of the random primers ensures that all possible sequences will be represented in the probe mixture. As a result, these primers are well suited for generating as long as possible (complete) transcripts of the RNA, transcribing the 5 'ends, in particular for long RNA templates. As an alternative you can also use Oligo(dT)-Primer > for reverse transcription. Oligo(dT) primer bind to the polyA tail of RNA and therefore transcription will start always at the 3'-end of the RNA. The guaranteed delivery quantity of 5OD, corresponding to approx. 76.1nmol, or 136.4μg, can be dissolved in 760μL DEPC water to give a concentration of 100pmol/μL (100μM), or approx. 180μg/μL. If using between 50ng and 250ng of the hexamers per 20μL reverse transcription reaction, 760μL (100μM) will be sufficient for 460 to 2280 reverse transcriptase reactions.

The better performance of the Genaxxon bioscience 5X B-Enhancer Solution compared to standard enhancers such as form amide, DMSO, TMCA or BSA especially when used with GC rich regions or templates with a high degree of secondary structures depends on its ability to lower the DNA melting temperature and its enhancing effect on the DNA polymerase. In detail, Betaine binds preferentially to AT rich sequences in the major groove, thereby stabilizing AT rich regions of the DNA. Because AT forms 2 hydrogen bonds and GC forms 3, the bonding of AT is less stable than the one of GC. As a consequence of the stabilizing effect of Betaine on AT bonding, the stability of AT bonding and GC bonding is brought close to an equal level. At the same time, Betaine has a sequence independent destabilizing effect on all DNA. Summarized, the Tm of AT rich and GC rich sequences are equalized and the overall Tm is lowered. Furthermore, Betaine aids the processivity of thermostable polymerases and reduces "pauses" in polymerization caused by secondary structure that can induce the polymerase to disassociate from the DNA strand.As Betaine has a decreasing effect on the melting temperature of DNA and primers the denaturation temperatures as well as primer annealing temperatures should be reduced by 1 – 5°C compared to the annealing temperature already used. The optimal annealing temperature should be determined individually for each reaction.

Green DNA Dye is a sensitive fluorescent dye for detection of dsDNA in qPCR (real-time PCR) and other applications. Because Green DNA Dye has greater sensitivity for dsDNA, it is especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. With exceptionally low background fluorescence Green DNA Dye is ideal for use with laser scanners. In the detection of double-stranded DNA in native polyacrylamide gels it is also much more sensitive than silver staining. When Green DNA Dye is free in solution, it emits a very low fluorescent signal. As soon as the dye binds to the double-stranded DNA, the signal increases significantly (thousand fold), which makes the fluorescent signal of the dye directly proportional to the amount of amplified dsDNA. Green DNA Dye is delivered as 100-time solution in DMSO. The concentration of Green DNA Dye is traditionally given as a dilution factor where the 1X dilution is used for staining of DNA gels. For PCR, a 0.2X dilution is the starting dilution for optimization.  Our customers rely on Genaxxon's Green DNA Dye and use the dye for research into the regulation of tumor suppressor genes or vaccinations/disease patterns triggered by infections with enteroviruses, among other things. Preparation of a working solution for realtime PCR reactions:Green DNA Dye is suitable for real-time PCR with a suggested concentration for short targets of 0.2X (short targets: 50 – 400bp). Dilute delivered 100-time stock solution with nuclease-free PCR grade water by 1:10 (pre-dilution) and add 0.4µL of this solution to a 20µL final volume (PCR reaction). The 10-time working solution has to be kept at -20°C and has to be renewed every 2-3 weeks latest. Green DNA dye is a low toxic fluorescent nucleic acid dye as an alternative to ethidium bromide. Are you looking for more non-toxic dyes for your gel electrophoresis? Then try SafeGel red stain or SafeGel green stain - the cost-effective alternatives to EtBr. For qPCR we offer various qPCR Master Mixes > for your needs, with or without ROX, e.g., Green Master Mix High ROX >, Green Master Mix No ROX > or Probe Master Mix No ROX for qPCR >. Online form for your qPCR master mix test sample >

ROX is used as passive reference dye to compensate for non-PCR related well to well variations in the fluorescence. Variations in fluorescence can occur e.g. due to well to well variation in light intensity, which depends on the optical construction of the PCR machine or due to pipetting variations. The ROX fluorescence does not change during the course of the PCR reaction but provide a stable baseline to which samples are normalized. The excitation and emission of the reference dye are 584nm and 612nm, respectively.The ROX concentration to be used depends on the Real Time PCR instrument or, more precisely, on the filter available. On earlier Real Time PCR machines there was no filter matching precisely to the ROX fluorescence. Therefore, high ROX concentrations are needed with these machines. Some newer machines do not need any internal standard to correct for light intensity. Nevertheless, an internal standard might still be useful to correct for pipetting variations.This dye has been qualified for use on instruments like the ABI PRISM® 7700, One-Step System, RotorGene and many others.Please consult the user manual of the real time PCR instrument with regard to appropriate levels of ROX. Ready to use qPCR Master Mixes with High ROX (500nM) >, Low ROX (50nM) > or without ROX > are also available from Genaxxon.

SuperHot Taq DNA polymerase is a chemicaly modified form of thermostable DNA polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first PCR cycle, the enzyme is not active and misprimed primers are not extended. As a result specifity and efficiency are increased by far compared to standard Taq DNA polymerase. Additionally, difficult targets with high GC-content can be amplified. Sensitivity improves multiplex PCR, an applied PCR technique that amplifies several specific targets simultaneously. The Genaxxon SuperHot Taq-Kit includes Genaxxon's SuperHot Taq Polymerase, buffer E, MgCl2, appropriate amounts of a 10mM dNTP-Mix sufficient for up to 500 / 2000 x 25µL PCR reactions (0.5 units Taq per reaction). All items are in shipped in separate tubes. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and favourable standard agarose (M3044) > we can offer additional products for your PCR.

PCR Hotstart Master mix with blue dye: for the visual control of pipetting steps after PCR, electrophoresis can be operated without the addition of loading buffer. This makes this PCR master mix saving time and money. This Hotstart Master Mix for qPCR is a premixed, ready-to-use solution containing chemically modified Taq DNA Polymerase, dNTPs, MgCl2, a blue dye and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR and qPCR (one tube, one pipetting step). Just add your primers and template DNA. The addition of the blue loading dye enables direct application of the PCR reaction onto a Gel. The Hotstart PCR Master Mix has been optimized for use in routine and diagnostic Hotstart PCR and qPCR for amplifying DNA template in the range of 0.2-4kb. The special formulation of our Hotstart Master Mix guarantees you reproducible results even after repeated freezing/thawing steps leading to good yields and sensitivity. The Hotstart Master Mix with blue dye is efficient (no left over of reagents), scalable from 10µL to 50µL and stable for 12 months. Hotstart Mastermix with blue dye for 200 x 25µL reactions (2.5mL). This Hotstart PCR master mix is also available as SuperHot Mastermix > enabling visualisation of each pipetting step. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and favourable standard agarose (M3044) > we can offer additional products for your PCR.

GreenMasterMix FAST Blue with 500nM ROX is optimised for realtime PCR assays in block systems. This 2X Mastermix is ready-to-use and contains all components for a successful and reliable qPCR with the exception of primer and template DNA. Advantages of the HotStart Taq DNA polymerase used in the Genaxxon GreenMasterMix: Hotstart technology enables setup of PCR mixture at room temperature. short initial denaturing time of not more than 2 minutes No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Green qPCR master mix Blue with 500nM ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP intercalating green fluorescent dye optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) This Mastermix is specially suited for the following instruments: Applied Biosystems® 5700, 7000, 7300, 7700, 7900, 7900 HT, Eppendorf Realplex4, StepOne™ and StepOnePlus™. Other realtime PCR master mixes from Genaxxon are: M3023 - GreenMastermix No ROX >, M3045 - ProbeMastermix No ROX >, M3011 - GreenMastermix Low ROX >, M3031 - ProbeMastermix Low ROX >, M3052 - GreenMastermix High ROX >, M3010 - ProbeMastermix High ROX >.

1.0mL PCR buffer without MgCl2 and without (NH)4SO4 for specific DNA amplification.

1.0mL PCR buffer with MgCl2 but without (NH4)2SO4 for specific DNA amplification.

1.0mL PCR buffer without MgCl2 but with (NH4)2SO4 for efficient DNA amplification. Standardbuffer for the Genaxxon Taq DNA Polymerase E, and HotStart Taq DNA Polymerase.

1.0mL PCR buffer with MgCl2 and with (NH4)2SO4 for efficient DNA amplification. Standard buffer for the Genaxxon Taq DNA Polymerase E, and HotStart Taq DNA Polymerase.   Please have also a look on our dNTP-Sets (100mM each dNTP) > or dNTP mixes with 2mM > or 10mM > concentration or our modified nucleotides, e.g. Biotin-11-dUTP >.

Custom made 10X PCR Buffer or other buffer solutions. Price is valid for common buffers containing MgCl2, KCl, Tris-HCl, gelatine, Tween 20. Buffer will be delivered in 250mL glas bottles, but we can also offer other bottle sizes. Buffer will be sterile filtered or autoclaved.

5X PCR Buffer Red is a ready to use PCR buffer, including all components for standard PCR applications. FEATURESAll in one buffer for highest convenienceDirect gel loading onto agarose gelsRed colour enables easy visualisation of pipetting and loadingDye front runs at 1000 - 2000 bp on DNA 0.5 - 1.5 % agarose DESCRIPTION5X PCR Buffer Red offers the same specification as our Taq-E buffer. It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or the annealing temperatures. The included red dye and density reagent, eliminates the need for loading dye as well as the time consuming sample preparation before electrophoresis.

Coral Red Buffer is a 10-time dye solution as supplement for PCR buffers. This additive contains Glycerol, EDTA, a red and a yellow dye. 5µL of this solution are added to 45µL of the PCR assay before the PCR reaction to stain it red. An alternative to this product is the Genaxxon bioscience ready-to-use RedMastermix M3029. The RedMastermix contains all incredients necessary for PCR including a red dye that enables to control the different pipetting steps.

1.0mL of a 25mM MgCl2 solution for PCR. Can be used for optimization of PCR reactions. Other volumes or concentrations on request. Please have also a look on our dNTP-Sets (100mM each dNTP) > or dNTP mixes with 2mM > or 10mM > concentration or our modified nucleotides, e.g. Biotin-11-dUTP >.

LyoPlex Multiplex PCR master mix for fast and reliable quantification of up to 5 cDNA or gDNA targets in a single tube by multiplex, real-time PCR. This specially optimized and lyophilized master mix promotes fast multiplex real-time PCR. The combination of the specially designed hot start DNA polymerase together with a unique PCR buffer system as a lyophilized ready-to-use master mix ensures highly sensitive qPCR together with a very convenient storage at room temperature. Our new lyophilized Multiplex master mix for fast and easy multiplexing minimizes the need for optimization and makes the development of multiplex PCR assays fast and easy.- Amplification of multiple targets in a single tube (tested for up to 5 targets)- All-in-one master mix for convenient multiplexing- High specificity, sensitivity and product yield- Easy reaction setup at room temperature- stable for 24 months at room temperature The Multiplex PCR master mix is a premixed, ready-to-use solution containing our highly specific chemically modified Taq DNA Polymerase, highly pure dNTPs, MgCl2, additives and a reaction buffer at optimal concentrations which enable reliable multiplex PCR, e.g. microsatellite, clonality or gene rearrangement analysis. Just add your primers and template DNA. The Multiplex HS Master mix 2X is shipped in aliquots of 1.25mL. Our Standard Agarose LE > and especially our high resolution Agarose Tiny > are ideally suited for the subsequent electrophoresis analysis. Other realtime master mixes for your realtime PCR experiments can be found here >. Examples of Multiplex applications: F. Javier Pérez-Pérez and Nancy D. Hanson, Detection of Plasmid-Mediated AmpC β-Lactamase Genes in Clinical Isolates by Using Multiplex PCR, J. Clin. Microbiol. June 2002 vol. 40 no. 6 2153-2162. doi: 10.1128/JCM.40.6.2153-2162.2002. Tamara B. Souzaa, Diego M. Lozerb, Sônia M. S. Kitagawab, Liliana C. Spanob, Neusa P. Silvac and Isabel C. A. Scaletskya, Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli. J. Clin. Microbiol. March 2013 vol. 51 no. 3 1031-1033, doi: 10.1128/JCM.02478-12.