Products for RT-PCR

Genaxxon offers a unique polymerase for RNA PCR (RT-PCR = Reverse transcription polymerase chain reaction) which is still regarded as the most sensitive method for the detection and quantification of mRNA.

Over the past few years the development of novel chemicals and instruments for qPCR has meant that the real time RT-PCR has become established as the method of choice for quantifying changes in gene expression. Furthermore, the real time RT-PCR is the preferred method for validating the results from array analysis and other techniques to track changes in gene expression.

Compared to other conventional techniques for the quantification of mRNA - Northern Blot analysis and RNase protection assays - in RT-PCR much lower amounts of sample are needed.

The standard enyzme for the synthesis of cDNA is Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase >. M-MuLV Reverse Transcriptase shows no 3´ → 5´ exonuclease activity.
For the synthesis of cDNA Genaxxon offers also a cDNA One-Step RT-PCR Kit >.

Troubleshooting & complaints feeedback form >
Frequently asked questions (FAQs) >

Genaxxon offers a unique polymerase for RNA PCR (RT-PCR = Reverse transcription polymerase chain reaction) which is still regarded as the most sensitive method for the detection and... read more »
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Products for RT-PCR

Genaxxon offers a unique polymerase for RNA PCR (RT-PCR = Reverse transcription polymerase chain reaction) which is still regarded as the most sensitive method for the detection and quantification of mRNA.

Over the past few years the development of novel chemicals and instruments for qPCR has meant that the real time RT-PCR has become established as the method of choice for quantifying changes in gene expression. Furthermore, the real time RT-PCR is the preferred method for validating the results from array analysis and other techniques to track changes in gene expression.

Compared to other conventional techniques for the quantification of mRNA - Northern Blot analysis and RNase protection assays - in RT-PCR much lower amounts of sample are needed.

The standard enyzme for the synthesis of cDNA is Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase >. M-MuLV Reverse Transcriptase shows no 3´ → 5´ exonuclease activity.
For the synthesis of cDNA Genaxxon offers also a cDNA One-Step RT-PCR Kit >.

Troubleshooting & complaints feeedback form >
Frequently asked questions (FAQs) >

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OneStep RT-PCR Kit
cDNA One-Step RT-PCR Kit
The cDNA RT-PCR One-Step Kit is designed for performing highly sensitive and specific RT-PCR. The kit is based on a genetically engineered reverse transcriptase with enhanced thermal stability resulting in an increased specificity, high...
From €127.05 *
M-MuLV Reverse Transcriptase RNase H negative
M-MuLV Reverse Transcriptase RNase H negative
The Genaxxon M-MuLV Reverse Transcriptase, encoded by Moloney Murine Leukemia Virus (M-MuLV RT) and expressed in E.coli is an RNA-dependent DNA polymerase lacking the RNase H activity. The enzyme synthesizes the cDNA first strand from a...
From €106.93 *