HotScriptase for the Detection of SARS-CoV-2, COVID-19

HotScriptase RT-PCR master mixes enable the amplification of RNA or DNA target sequences with quick and easy PCR protocols, even without a separate isothermal reverse transcription step. The Genaxxon Hotscriptase Probe Master Mix was successfully used by several customers for the detection of corona viruses (SARS-CoV-2, COVID-19).

Genaxxon offers a unique polymerase for RNA PCR (RT-PCR = Reverse transcription polymerase chain reaction) which is still regarded as the most sensitive method for the detection and quantification of mRNA. Our HotScriptase is a major step forward for RT-PCR, since even stable tertiary RNA structures are no longer a problem due to high annealing temperatures 

Over the past few years the development of novel chemicals and instruments for qPCR has meant that the real time RT-PCR has become established as the method of choice for quantifying changes in gene expression. Furthermore, the real time RT-PCR is the preferred method for validating the results from array analysis and other techniques to track changes in gene expression.

Compared to other conventional techniques for the quantification of mRNA - Northern Blot analysis and RNase protection assays - in RT-PCR much lower amounts of sample are needed.

The standard enyzme for the synthesis of cDNA is Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase >. M-MuLV Reverse Transcriptase shows no 3´ → 5´ exonuclease activity.
For the synthesis of cDNA Genaxxon offers also a cDNA One-Step RT-PCR Kit >.

Troubleshooting & complaints feeedback form >
Frequently asked questions (FAQs) >

HotScriptase RT-PCR master mixes enable the amplification of RNA or DNA target sequences with quick and easy PCR protocols, even without a separate isothermal reverse transcription step. The... read more »
Close window
HotScriptase for the Detection of SARS-CoV-2, COVID-19

HotScriptase RT-PCR master mixes enable the amplification of RNA or DNA target sequences with quick and easy PCR protocols, even without a separate isothermal reverse transcription step. The Genaxxon Hotscriptase Probe Master Mix was successfully used by several customers for the detection of corona viruses (SARS-CoV-2, COVID-19).

Genaxxon offers a unique polymerase for RNA PCR (RT-PCR = Reverse transcription polymerase chain reaction) which is still regarded as the most sensitive method for the detection and quantification of mRNA. Our HotScriptase is a major step forward for RT-PCR, since even stable tertiary RNA structures are no longer a problem due to high annealing temperatures 

Over the past few years the development of novel chemicals and instruments for qPCR has meant that the real time RT-PCR has become established as the method of choice for quantifying changes in gene expression. Furthermore, the real time RT-PCR is the preferred method for validating the results from array analysis and other techniques to track changes in gene expression.

Compared to other conventional techniques for the quantification of mRNA - Northern Blot analysis and RNase protection assays - in RT-PCR much lower amounts of sample are needed.

The standard enyzme for the synthesis of cDNA is Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase >. M-MuLV Reverse Transcriptase shows no 3´ → 5´ exonuclease activity.
For the synthesis of cDNA Genaxxon offers also a cDNA One-Step RT-PCR Kit >.

Troubleshooting & complaints feeedback form >
Frequently asked questions (FAQs) >

Close filters
  •  
  •  
 
from to
  •  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
No results were found for the filter!
NEW
OneStep RT-PCR Kit cDNA One-Step RT-qPCR 2X GreenMastermix
The cDNA One-Step RT-qPCR 2X master mix was developed for quantitative real-time analyzes of RNA templates using EvaGreen®. The ready-to-use mixture is based on a genetically modified reverse transcriptase with improved thermal...
From €102.25 *
NEW
OneStep RT-PCR Kit cDNA One-Step RT-qPCR 2X ProbeMastermix
The cDNA One-Step RT-qPCR 2X ProbeMastermix was developed for quantitative real-time analyzes of RNA templates with double-labelled fluorescence probes. The ready-to-use mixture is based on an optimized genetically modified reverse...
From €102.25 *
Hint!
NEW
HotScriptase - heat stable reverse Transcriptase HotScriptase Probe RT-qPCR master mix without ROX
HotScriptase Probe master mix for RT-PCR without an isothermal intermediate step, thereby saving time and money: HotScriptase Probe master mix was successfully used by several customers for the detection of RNA viruses like Sika or...
From €20.00 *
NEW
HotScriptase RT-PCR direct from cells HotScriptase RT PCR from cell master mix
HotScriptase RT Cell, a 2-time master mix that has been specifically optimized for direct-from-cell applications. Skip the time-consuming and expensive RNA purification and cell lysis steps. With the new HotScriptase RT 2X Cell master...
From €35.00 *
HotScriptase RT-PCR without RNA purification HotScriptase RT polymerase
"One-Step" RT-PCR without isothermal reverse transcription step. Using HotScriptase RT is very time and cost efficient as HotScriptase RT is a reverse transcriptase and a DNA polymerase combined in one highly thermostable enzyme. (find...
From €11.00 *
Hint!
NEW
HotScriptase - heat stable reverse Transcriptase HotScriptase RT-qPCR COVID-19 CDC Probe Assay Kit
The HotScriptase RT-qPCR SARS-CoV-2 CDC Probe Assay Kit is a real-time RT-PCR-based detection system for the targets N1 and N2 of the nucleocapsid from the SARS-CoV-2-Corona virus (2019-nCoV), as well as for the control target RNASeP....
From €205.00 *
M-MuLV Reverse Transcriptase RNase H negative M-MuLV Reverse Transcriptase RNase H negative
The Genaxxon M-MuLV Reverse Transcriptase, encoded by Moloney Murine Leukemia Virus (M-MuLV RT) and expressed in E.coli is an RNA-dependent DNA polymerase lacking the RNase H activity. The enzyme synthesizes the cDNA first strand from a...
From €110.14 *
NEW
OneStep RT-PCR Kit One-Step cDNA RT-PCR Kit (2X) - improved
Improved version of our kit. Now coming as a 2-time ready-to-use RT-PCR master mix. Our cDNA RT-PCR One-Step Kit is designed for performing highly sensitive and specific RT-PCR. The kit is based on a genetically engineered reverse...
From €325.00 *