HotScriptase for the Detection of SARS-CoV-2, COVID-19

HotScriptase RT-PCR MasterMixes enable the amplification of RNA or DNA target sequences with quick and easy PCR protocols, even without a separate isothermal reverse transcription step. The GENAXXON Hotscriptase Probe MasterMix was successfully used by several customers for the detection of corona viruses (SARS-CoV-2, COVID-19).

GENAXXON offers a unique polymerase for RNA PCR (RT-PCR = Reverse transcription polymerase chain reaction) which is still regarded as the most sensitive method for the detection and quantification of mRNA. Our HotScriptase is a major step forward for RT-PCR, since even stable tertiary RNA structures are no longer a problem due to high annealing temperatures 

Over the past few years the development of novel chemicals and instruments for qPCR has meant that the real time RT-PCR has become established as the method of choice for quantifying changes in gene expression. Furthermore, the real time RT-PCR is the preferred method for validating the results from array analysis and other techniques to track changes in gene expression.

Compared to other conventional techniques for the quantification of mRNA - Northern Blot analysis and RNase protection assays - in RT-PCR much lower amounts of sample are needed.

The standard enyzme for the synthesis of cDNA is Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase >. M-MuLV Reverse Transcriptase shows no 3´ → 5´ exonuclease activity.
For the synthesis of cDNA Genaxxon offers also a cDNA One-Step RT-PCR Kit >.

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