RNA- DNA- Protein-Purification

Mini DNA purification columns (40µL - 100µL elution volume) for DNA purification kits of different suppliers, e.g. Qiagen, Zymo, Invitrogen, Promega, etc.. These columns are a convenient and cheap alternative if buffers of already existing DNA purification kits are left but columns are out. Columns can be used for Genaxxon kits, but also for such of other suppliers. Content: 50 columns. DNA, RNA, DNase-free columns for DNA purification. Binding capacity: up to 25µg DNA. Yields: 99% to 90% depending on DNA fragment length (<100bp or >10kbp possibly reduced yield). Purity: A260/A280 ratio: 1.7 – 1.9. Time required: 5 - 10 min.

RNA Purification Mini Spin Columns for RNA purification kits of different suppliers. These RNA purification spin columns are a convenient and cheap alternative if buffers of already existing RNA purification kits are left but columns are out. For purification of total RNA. RNA can be used for: Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array analysis, next-generation sequencing. Elution volume of the RNA purification spin columns: 30-100µL, main sample types: tissue, cells, sample amount: 1 - 30mg. Additional Genaxxon offer also ceramic beads for rapid cell disintegration. You can reorder our RNA > and DNA purification columns > for the isolation of total RNA or DNA from enzymatic reactions or ceramic beads > separately. These articles can also be used with buffers/kits from other suppliers. With our HotScriptase RT Polymerase >, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step.

Genomic DNA purification columns with cap for purification of Plasmid-DNA. The Genaxxon columns can also be used for left-over buffer from purification kits of other suppliers than Genaxxon, e.g. for Qiagen kits cat# 69504 and 69506. These columns are a convenient and cheap alternative if buffers of already existing DNA purification kits are left but columns are out.Content: 50 columns.

Ceramic beads for the lysis of tissue from a wide range of sample material, e.g. for RNA extraction. The kit contains 100 preps with 1.4mm ceramic beads (zirconia) and comes pre-proportioned to 1g in 2mL standard screw cap tubes. The beads are suitable for the homogenization of "soft" tissue from lung, liver, brain, kidney, spleen, skin, cells from cell culture, as well as for plant tissues such as e.g. Leaves or even whole insects. The usabl sample amount ranges from up to 200mg tissue, up to 200μL of cells suspended in water or isotonic saline solution, or up to 100mg wet weight of tissue culture cells grown in suspension (e.g., ≤10E7 mammalian cells). The beads can be used for a variety of samples and applications, e.g. B. for veterinary diagnostics (BSE screening), pharmacology and toxicology (drug detection), criminology and forensic medicine, food industry (GMO detection), microbiology in the environment (microorganism lysis). as well as the isolation of nucleic acids and proteins. They are chemically inert and do not bind nucleic acids. The beads are comparable with MP Biomedical No. 116913100 (equivalent to Lysing Matrix D) and are compatible with most commercially available homogenizers, like FastPrep® (MP Biomedicals), MagNA Lyser Instrument (Roche). You can reorder our RNA > and DNA purification columns > for the isolation of total RNA or DNA from enzymatic reactions or ceramic beads > separately. These articles can also be used with buffers/kits from other suppliers. With our HotScriptase RT PCR master mix >, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step. 

GENAzol is a ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA and proteins from biological samples. The single-phase solution of aqueous guanidine isothiocyanate solution can be used to isolate RNA, DNA and proteins in various fractions from cell and tissue samples, e.g. from humans, animals, plants, yeasts, bacteria or viruses (usually within one hour). Properties of GENAzol:- Parallel isolation of RNA, DNA and protein from the same sample- Superior suitability for lysis even with difficult samples- Optimized formulations and protocols for tissues, cells, blood, viruses and bacteria. Reliable purification of RNA from samples of different volumes and sourcesGENAzol is also suitable for small amounts of tissue (50–100mg) and cells (5× 10E6) as well as for larger amounts of cells (>10E7) and contains protocols for the purification of RNA from human, animal, plant or bacterial Rehearse. GENAzol maintains the integrity of the RNA due to the very effective inhibition of RNase activity when cells and components are dissolved during sample homogenization. GENAzol enables the simultaneous processing of a large number of samples. The entire isolation step is usually completed in 1 hour. The total RNA isolated with GENAzol is free from protein and DNA contamination. Procedure for the isolation of RNA, DNA and proteinsGENAzol enables sequential precipitation of RNA, DNA and proteins from a single sample. After homogenizing the sample with GENAzol, chloroform is added and the homogenate separates into a clear aqueous top layer (which contains RNA), a boundary layer and a organic bottom layer (with DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA precipitation from the organic boundary layer takes place with ethanol. Finally, the protein from the phenol-ethanol supernatant is precipitated with isopropanol. Precipitated RNA, DNA or protein is washed to remove impurities and then resuspended for use in subsequent applications.This is a dangerous good with UN number UN2821. Not for sale outside the European Union. UFI-Code: 7H2D-1Q71-Q00M-D0WU

Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments We use magnetic beads with a ferrimagnetic core and an agarose coating coupled to a ligand of choice, our beads meet our own high quality standards. They enable fast and easy purification steps, which can be automated. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Genaxxon MagBeads are ferrimagnetic agarose beads coupled to a chelating ligand (IDA or NTA) coordinating nickel or cobalt ions. All offered ligand-metal systems efficiently bind histidine-tagged proteins. With a binding capacity of up to 40mg protein per mL (Ni-IDA) and 70mg per mL (Ni-NTA) of settled MagBeads, Genaxxon Ni-IDA MagBeads are comparable to equivalent beads from alternative providers.

NTA-Agarose consists of the tetradentate chelating agent, nitrilotriacetic acid (NTA), covalently coupled agarose beads, and is loaded with Co2+ ions. In processing recombinant fusion proteins from eukaryotes or in the case of special protein complexes, Genaxxon Co-NTA Agarose should always be used offering the possibility of higher protein purity levels. Both metal ions feature differing spatial properties and electronic binding potentials. While Co2+ binds only domains adjacent to residual histidine and is thus highly selective for 6×histidine-tagged fusion proteins, Ni2+ ions are less specific and can bind residual histidine outside the polyhistidine tag, which may result in higher contamination with foreign proteins. The unique production process yields a Co-NTA Agarose that exhibits a higher protein binding capacity than that of leading competitor products. Genaxxon Co-NTA Agarose is very robust in the presence of DTT and EDTA. In a stability test, Genaxxon Co-NTA Agarose was exposed to increasing concentrations of DTT or EDTA for 1h. Thereafter, the resins were used to purify E. coli-expressed GFP-His in gravity columns. The binding capacity of the resin decreased in the presence of both DTT and EDTA but the decay rate was shallow. Genaxxon NTA Agarose is supplied as a 50% buffered suspension in 20% Ethanol.

Plasmid DNA purification columns with cap for purification of Plasmid-DNA. The Genaxxon columns can also be used for left-over buffer from purification kits of other suppliers than Genaxxon. These columns are a convenient and cheap alternative if buffers of already existing DNA-purification kits are left but columns are out. Content: 50 columns.

Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments We use magnetic beads with a ferrimagnetic core and an agarose coating coupled to a ligand of choice, our beads meet our own high quality standards. They enable fast and easy purification steps, which can be automated. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Genaxxon MagBeads are ferrimagnetic agarose beads coupled to a chelating ligand (IDA or NTA) coordinating nickel or cobalt ions. All offered ligand-metal systems efficiently bind histidine-tagged proteins. With a binding capacity of up to 40mg protein per mL (Ni-IDA) and 70mg per mL (Ni-NTA) of settled MagBeads, Genaxxon Ni-IDA MagBeads are comparable to equivalent beads from alternative providers.

G2 DNA/RNA Enhancer for improved DNA/RNA extraction!The G2 DNA/RNA Enhancer is developed to increase the yield of microbial DNA during DNA extraction from difficult matrices for example clay and soil samples. The primary function of G2 DNA/RNA Enhancer is to relieve inhibitory DNA - particle or other inhibitory DNA complexes. FEATURES- Inhibition of DNA and RNA sorption to clay particles- At least 2-10 fold increased yield of microbial DNA and RNA.- No trace of endonuclease-, nicking-, exonuclease- or RNase activity- Patent EP2 443 251 DESCRIPTIONG2 DNA/RNA Enhancer is available freeze dried with 0.1mm or 1.4mm ceramic beads in a 2mL tube. G2 DNA/Enhancer is also available in liquid format in three packing sizes including either 10, 50 or 100 reactions of 500µL. G2 DNA/RNA Enhancer should be used in combination with either a standardised extraction methods or commercial purification kits intended for DNA & RNA extraction. G2 DNA/RNA ENHANCER INCREASES DNA YIELD FROM SOIL SIGNIFICANTLY. G2 DNA/RNA Enhancer is available with 0.1mm ceramic beads (> A4201xx) or with 1.4mm ceramic beads (> A4214xx) and as solution (> A4200xx).Protocol for using G2 DNA/RNA Enhancer BeadsHow to use G2 DNA/RNA Enhancer in combination with commerical extraction kits:This protocol serves as a guideline for extraction of DNA and RNA from difficult matrices, such as soil clays, soils, activated coal etc. when using the G2 DNA/RNA Enhancer (G2) in combination with a commercial DNA or RNA extraction kit intended for soil.

Ni-NTA agarose was developed for affinity purification of proteins bearing a polyhistidine label. This affinity chromatography matrix is based on BioWorks workbeads consisting of 7.5% cross-linked agarose. The material is highly porous to allow optimal protein interaction. Cross-linked agarose is also physically very stable and suitable for low pressure purification processes with flow rates up to 6mL/min (optimal 0.5 - 2mL/min). Our Ni-NTA agarose has a very homogeneous size with a mean particle diameter of 40μm, resulting in a high degree of reproducibility between individual purification runs. Ni-NTA agarose consists of cross-linked agarose to which tetradendritic nitrilotriacetic acid (NTA) loaded with Ni2+ ions is covalently bound. The binding capacity varies with different proteins based on their specific properties, but it is at least 50mg target protein per mL agarose gel. The resin is ideally suited for batch, spin and column applications of any scale and offers a high degree of reproducibility between individual purification approaches with a very homogeneous size distribution around a mean particle diameter of 40µm. The Ni-NTA agarose is supplied as a buffered suspension in 50% ethanol. For example, 1x S5377.0010 corresponds to a suspension of 10mL resin and 10mL water with 20% EtOH. Total supplied volumes is 20mL.

G2 DNA/RNA Enhancer for improved DNA/RNA extraction!The G2 DNA/RNA Enhancer is developed to increase the yield of microbial DNA during DNA extraction from difficult matrices for example clay and soil samples. The primary function of G2 DNA/RNA Enhancer is to relieve inhibitory DNA - particle or other inhibitory DNA complexes. FEATURES- Inhibition of DNA and RNA sorption to clay particles- At least 2-10 fold increased yield of microbial DNA and RNA.- No trace of endonuclease-, nicking-, exonuclease- or RNase activity- Patent EP2 443 251 DESCRIPTIONG2 DNA/RNA Enhancer is available freeze dried with 0.1mm or 1.4mm ceramic beads in a 2mL tube. G2 DNA/Enhancer is also available in liquid format in three packing sizes including either 10, 50 or 100 reactions of 500µL. G2 DNA/RNA Enhancer should be used in combination with either a standardised extraction methods or commercial purification kits intended for DNA & RNA extraction. G2 DNA/RNA ENHANCER INCREASES DNA YIELD FROM SOIL SIGNIFICANTLY. G2 DNA/RNA Enhancer is available with 0.1mm ceramic beads (> A4201xx) or with 1.4mm ceramic beads (> A4214xx) and as solution (> A4200xx). Protocol for using G2 DNA/RNA Enhancer BeadsHow to use G2 DNA/RNA Enhancer in combination with commerical extraction kits:This protocol serves as a guideline for extraction of DNA and RNA from difficult matrices, such as soil clays, soils, activated coal etc. when using the G2 DNA/RNA Enhancer (G2) in combination with a commercial DNA or RNA extraction kit intended for soil.

Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments We use magnetic beads with a ferrimagnetic core and an agarose coating coupled to a ligand of choice, our beads meet our own high quality standards. They enable fast and easy purification steps, which can be automated. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Genaxxon MagBeads are ferrimagnetic agarose beads coupled to a chelating ligand (IDA or NTA) coordinating nickel or cobalt ions. All offered ligand-metal systems efficiently bind histidine-tagged proteins. With a binding capacity of up to 40mg protein per mL (Ni-IDA) and 70mg per mL (Ni-NTA) of settled MagBeads, Genaxxon Ni-IDA MagBeads are comparable to equivalent beads from alternative providers.

The non-toxic DNA Q-Extraction Solution provides fast and easy extraction of nucleic acids from various mammalian tissues (e.g. mouse tails, ear snips, liver, kidney, lung), saliva and bacteria. The PCR-ready nucleic acid is extracted, using one tube and one reaction, in as little as 8 minutes. The one-step lysis is performed in either a thermocycler or heating block and is divided into two simple heating steps. The extracted DNA is then ready for PCR without further handling such as vortex, centrifugation or dilutions. The obtained DNA extract is stable at -20°C for up to one week and at -80°C for long term storage.Recent user data confirm that the DNA Q-Extraction Solution can also be successfully used with fungi samples, such as yeast and mold. The standard 8-minute protocol yields PCR-ready DNA without the need for additional clean-up or purification steps. This expands the range of applications and makes the DNA Q-Extraction Solution a versatile tool for rapid DNA extraction, even from more challenging sample types. For optimal genotyping results we recommend to use our DNA Q-Extraction Solution together with our Red Mastermix Master Mix (2X) for PCR (M3029) or our SuperHot Mastermix Blue (2X) (M3007b). E.g. use 2µL to 5µL of the Q-Extract solution for a 25µL PCR reaction. For plant tissue we recommend using our specific Plant DNA Q-Extraction Solution (M3230). Features:- One-reagent set-up- Minimal handling- Rapid 8-minute protocol- PCR-ready DNA- no sample / yield loss- DNA extracts from mammalian tissues and bacteria- Non-toxic reagents- Scalable set-up- Automation-friendly Extraction ProtocolPreparation of DNA extraction should be performed in a separate area from that used for setting up the PCR reaction. 1. Thaw DNA Q-Extraction Solution. For the first time use, aliquot the DNA Q-Extraction solution into smaller volumes. (DNA Q-Extraction Solution has a cloudy appearance).2. Add your sample to a tube containing 100µL DNA Q-Extraction Solution. Recommended sample sizes are shown in Table 1 of the data sheet.3. Vortex the tube containing the sample and the DNA extraction solution for 15 sec. Make sure that the sample is completely covered by the DNA Q-Extraction Solution.4. Transfer the tube to a heat block or a thermal cycler and incubate for 1. 65 °C for 6 min2. 98 °C for 2 min3. 4 °C (or cool down on ice) The DNA extract is now ready for PCR. Anwendungsbeispiele:Efficient Amplification of DNA extracts from various mammalian tissue.DNA Q-Extraction Solution was used together with our RedMastermix (M3029 >) to extract and amplify genomic DNA from various mammalian tissues. For DNA extraction, 0.5-10 mg tissue or 20µL salvia was added to 100µL of DNA Q-Extraction Solution.M: DNA marker Iqon Low DNA Ladder. Lane 1-5: Different mouse tissues as depicted, GAPDH (266 bp). Lane 6: Chicken muscle tissue, HRPT1 (245 bp) and Lane 7: Human saliva, DMD17 (415 bp). DNA Q-Exctract Solution works over a wide range of sample sizesIn order to investigate the flexibility of DNA Q-Extraction Solution protocol, varying amounts of chicken muscle tissues were added to 100µL of DNA Q-Extraction Solution. The extraction was conducted as specified in the datasheet for DNA Q-Extraction Solution. The extracted DNA was subjected to PCR using the Genaxxon RedMastermix (M3029 >). The results show that the DNA Q-Extraction protocol provides the user with a high degree of flexibility over a wide range of applied sample amount.M: DNA marker Iqon Low DNA Ladder. Lanes 1-28: Varying amounts (mg) of chicken muscle tissue, HRPT1 (245 bp). Application Notes:application note genotyping with m3231_dna_q_extraction_solution_en_vs250527application note realtime pcr with m3230 plant dna_q_extraction_solution_en_vs250527

G2 DNA/RNA Enhancer for improved DNA/RNA extraction!The G2 DNA/RNA Enhancer is developed to increase the yield of microbial DNA during DNA extraction from difficult matrices for example clay and soil samples. The primary function of G2 DNA/RNA Enhancer is to relieve inhibitory DNA - particle or other inhibitory DNA complexes. FEATURES- Inhibition of DNA and RNA sorption to clay particles- At least 2-10 fold increased yield of microbial DNA and RNA.- No trace of endonuclease-, nicking-, exonuclease- or RNase activity- Patent EP2 443 251 DESCRIPTIONG2 DNA/RNA Enhancer is available freeze dried with 0.1mm or 1.4mm ceramic beads in a 2mL tube. G2 DNA/Enhancer is also available in liquid format in three packing sizes including either 10, 50 or 100 reactions of 500µL. G2 DNA/RNA Enhancer should be used in combination with either a standardised extraction methods or commercial purification kits intended for DNA & RNA extraction. G2 DNA/RNA ENHANCER INCREASES DNA YIELD FROM SOIL SIGNIFICANTLY. G2 DNA/RNA Enhancer is available with 0.1mm ceramic beads (> A4201xx) or with 1.4mm ceramic beads (> A4214xx) and as solution (> A4200xx).

The PureIT ExoZAP - PCR Clean-Up reagent from Ampliqon can be used for enzymatic cleanup of your PCR products without the need for purification columns. With PureIT ExoZAP PCR products from less than 100bp to over 20kbp can be purified from primers and unincorporated dNTPs with no sample loss. The combination of HL-ExoI and a recombinant Shrimp Alkaline Phosphatase (rSAP) hydrolyzes excess primers and nucleotides in a single step within the PCR reaction tube! ZAP-SAPexo purified samples are ready for use in downstream applications such as DNA sequencing or SNP analysis (single nucleotide polymorphism) after a simple 5 minute incubation. Advantages:• Fast. Only 5 minutes without centrifugation.• Just one pipetting step for pure DNA sequencing samples!• Simple enzymatic removal of excess primers and unincorporated nucleotides.• Minimal loss of your PCR sample. Up to 100% recovery. Our innovative PureIT ExoZAP solution offers a simple method to purify your PCR reactions from primers and unincorporated nucleotides. Only one pipetting step followed by a min. 2 minutes incubation at 37°C (digestion) and a min. 3 (-10) minutes incubation at 80°C (inactivation). One-step solution provides a simple way to enzymatically purify a PCR reaction. Just programme your PCR cycler after the PCR accordingly, pipet the PureIT ExoZAP reagent to each tube or well, start Cycler and you will have your purified PCR samples after 5 minutes ready for use in sequencing or SNP analyis. After the enzymatic reaction with PureIT ExoZAP, primers and dNTPs will no longer interfere with DNA sequencing or SNP analysis, for example with our SNP Pol DNA polymerase >. Using PureIT ExoZAP offers the possibility to proceed with the sequencing reaction after the BigDye™ Cycle Sequencing reaction without the need for addition time consuming purification methods, e.g. with Dye Terminator purification kits. PureIT ExoZAP PCR CleanUp IMPROVES QUALITY OF DNA SEQUENCING PCR product of 866 bp was amplified using TEMPase DNA Polymerase in Ammonium Buffer. The PCR product was spiked with dNTP’s and primers and then treated (A) without PureIT ExoZAP PCR CleanUp (B) with PureIT ExoZAP PCR CleanUp (2 +3 min) and (C) with an enzymatic PCR clean-up reagent from a competting brand (1+ 4 min) prior to Sanger sequencing. All samples were analyzed in triplicates and according to manufactures recommendations. Sanger sequencing result was compared by graphically plotting the number of base calls with certain quality values (Phred score). Treatment of PCR products with PureIT ExoZAP significantly improves the quality of DNA sequencing. Furthermore, PureIT ExoZAP PCR CleanUp showed PCR clean-up results equivalent to the competing PCR clean-up reagent. *Data with Quality values less than 10 is not included. Sanger sequencing results of the 866 bp PCR product. The depicted electropherograms are either after treatment with PureIT ExoZAP PCR CleanUp (top) or untreated (bottom). Sequence shown is approximately the region from 250 - 300 bases. Treatment of spiked PCR product with PureIT ExoZAP PCR CleanUp significantly improves the quality of Sanger Sequencing. Treatment enhances signal intensity and eliminates background interference and base miscalls.

Nickel-loaded agaroses are the most frequently used products for the efficient purification of 6×histidine-tagged recombinant fusion proteins. Because Ni2+ ions show high affinity for 6×histidine-tagged fusion proteins, extremely high yields result from purification of these fusion proteins from the lysates. However, if purification takes place from lysates containing foreign protein chains with repeated histidine elements, selectivity with respect to 6×histidine-tagged fusion protein decreases as non-tagged histidine-containing proteins increasingly also bind to the Ni-IDA agarose. In this case the use of Genaxxon Co-IDA Agarose is recommend (cat#: S5370), which features far higher selectivity with respect to 6×histidine-tagged fusion protein. Genaxxon Ni-IDA Agarose has high chemically and pH stability and enables purification directly from raw lysates. Genaxxon Ni-IDA Agarose can be regenerated several times with nickel sulfate solution for re-use. Features of Ni-IDA-Agarose - a highly efficient and favorable way for the separation of 6-His tagged fusion proteins. Highlights: - ideal for grafity-flow and batch procedures - no expensive purification system necessary - consistent performance - for native and denaturing conditions. The Genaxxon Ni-IDA Agarose consists of cross-linked Agarose, covalently bound to Iminodiacetic acid loaded with Ni2+-ions. Binding capacity: up to 50mg 6xHis tagged fusion protein per mL Agarose. Application: Affinity chromatography of 6-His tagged fusion proteins in batch or grafity flow procedure. Ni-IDA Agarose is supplied as a 50% buffered suspension with 20% Ethanol.

Genaxxon´s Total RNA Purification Kit - Tissue is designed for the rapid and efficient purification of high quality RNA from 1-30mg of tissue (fresh or frozen) or up to 25mg Paraffin embedded tissue. The isolation protocol and buffer formulations were optimised for high isolation efficiency and purity of RNA. The Total RNA Purification Kit utilises spin columns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. The isolation procedure consists of 6 steps. In the first isolation step, the tissue is homogenized in order to disintegrate intercellular bonds (epithelial tissue) and fragmentize high-molecular proteins (muscle or connective tissue). Then the homogenate is lysed. Any RNases are inactivated. The three-step washing stage effectively removes impurities and enzyme inhibitors. The purified RNA is eluted using a low ionic strength buffer or RNase-free water and can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR and so forth. Genaxxon offers also ceramic beads > for rapid cell disintegration and other RNA purification kits >. With our HotScriptase RT Polymerase >, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step. Here you will find further Purification kits >.

Empty Spin Columns for standard protein purification procedures, e.g. affinity chromatography with Ni-IDA, Ni-NTA, Co-IDA oder Co-NTA agaroses. The smallest column size does have a capacity of 0.5mL (S) and are ideal for protein optimisation process screenings. Our L columns (20mL) are a very cost effecitve option if you like to order IDA- or NTA-agarose in bulk and fill columnes by yourself. Our empty spin columns are offered in the sizes: 0.5mL (S), and 20mL (L). Easy Handling. Useable under native and denaturing conditions. For being able to use our spin columns you need a centrifuge with fixed angle (45° for our 600µL columns), or with a swing bucket rotor (for the 20mL columns).

Genaxxon bioscience offers pre-packed Ni2+ IDA columns in ready-to-use format for His-tag protein purification processes by gravity flow. Fast purifications with good yield of target proteins are obtainable by this method. Both matrices Ni2+- and Co2+-IDA-Agarose and 2 different bed volumes (1mL, 5mL) are available supplied as suspension in 20% ethanol. Frit Pore Size 20µm - 6% crosslinked agarose (Sepharose®CL-6B) -Matrix Stability in all commonly used reagents for protein purifications - Activating Agent Epichlorohydrin -Bed Volume Agarose 1mL (for L-Column), 5mL (for XL-Column) - Autoclavable at 121°, 30 min. - Capacity ca. 28 µmol Ni2+/mL gel, ca. 25 µmol Co2+/mL gel.

CentriPure P100 columns are pre-hydrated gel filtration columns for protein purification and desalting. CentriPure P100 Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins. The gel matrix of CentriPure is Zetadex-25, a beaded composite material developed by emp Biotech composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Zetadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for Zetadex-25 is 5 kD for proteins. Proteins larger than 5 kD in a sample volume of up to 10mL can be purified with an elution volume of 12mL to 15mL.

CentriPure P50 columns are pre-hydrated gel filtration columns for protein purification and desalting. Processes sample volumes of 5mL. CentriPure P50 Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins. The gel matrix of CentriPure is Zetadex-25, a beaded composite material developed by emp Biotech composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Zetadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for Zetadex-25 is 5 kD for proteins. Proteins larger than 5 kD in a sample volume of 5 mL can be purified with an elution volume of 6 to 8 mL.

Die CentriPure CP-0219 columns are specially designed for purification and desalting of oligonucleotides longer than 20 base pairs and from proteins greater than 25 kDa with minimal dilution. The gel bed has a volume of 0.5mL. Samples with a volume up to 100µL can be processed. Optimal purification and recovery is obtained with a sample volume of 50µL. Because the columns are rehydrated shortly before use, their shelf life (non-hydrated) at +15°C to +30°C is several years. More than 99% of impurities as salts, dNTPs or Dye terminators can be removed by our CentriPure columns. As a result you will get consitent and reliable signals from base 1 to 700. Applications: - Purification of DNA sequencing reactions.- Removal of free and labelled dNTP’s from DNA/RNA polymerisation reactions (e.g. RT-PCR, PCR, nick translation).- Purification/desalting of proteins. Removal of traces of phenol or exchange of buffer salts, as in multiple restriction digestions. These columns are far superior - in ease of use, speed, and non-toxicity - to such common techniques as phenol/chloroform extractions and ethanol precipitations.

CentriPure P5 columns are pre-hydrated gel filtration columns for protein purification and desalting. Process sample volume of 500µL. CentriPure P5 Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins. The gel matrix of CentriPure is Zetadex-25, a beaded composite material developed by emp Biotech composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Zetadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for Zetadex-25 is 5 kDa for proteins. Proteins larger than 5 kDa in a sample volume of up to 650µL can be purified with an elution volume of 650 to 900µL.

For the rapid and reliable removal of excess dye terminators, primer, dNTPs and other small molecules from completed DNA sequencing reactions, e.g. from BigDye™ Cycle Sequencing reactions. Samples from 20µL can be processed reliably and consistently in under 10 minutes. Removal of salts, dNTPs and other unwanted low molecular weight impurities (ammonia, haptens, biotin, etc.) can be accomplished by more than 98%. High, uniform signal intensity for base calls from base 1 to over 700. The plates can be stored at +2°C to +8°C for 6 months. Gelbed volume available in 400µL and 200µL. The CentriPure plates will be a cost effective alternative to the recommended Centri-Sep plates. CP-0101 plates are normally produced for each order for maximum shelf life of 6-7 months. The delivery time is therefore normally between 10 and 14 days after official receipt of the order.

Glycogen is a branched carbohydrate that can be used as a co-precipitant in nucleic acid isolation. Features of Glyogen: • Ideal for RT-PCR• Increases Pellet-Mass• Quantitative recovery in case of low/very low nucleic acid amounts (ng/mL).• Prevents pellet loss in nuclease protection assays.• 260/280nm measurements are unaffected by Glycogen. Co-precipitants are inert substances (Glycogen or linear Acrylamide) to aid in the recovery of nucleic acids after alcohol precipitation. They are almost indispensable for the quantitative recovery of small quantities of nucleic acids from diluted solutions. Often, the use of such molecules is not desirable for any reason other than the visualization of the pelleted after centrifugation. When used at a final concentration of 50 to 150µg/mL, glycogen will co-precipitate with nucleic acids in the presence of 0.5M ammonium acetate and isopropanol or ethanol.

Hydrated gel filtration columns for protein purification and desalting. Processes sample volumes of 150 to 300µL. CentriPure 2 Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins. The gel matrix of CentriPure is Zetadex-25, a beaded composite material developed by emp Biotech composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Zetadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for Zetadex-25 is 5 kD for proteins. Proteins larger than 5 kD in a sample volume of up to 200µL can be purified with an elution volume of 200 to 350µL.

CentriPure Z25 Mini Spin columns are prehydrated (in water) Mini Columns used for quick and efficient desalting, buffer exchange and/or removal of dyes and small molecules (haptens, biotin, ammonium, etc.) from proteins greater than 5 kD. Purified proteins are eluted into pure, deionized water (Caution! Some proteins may precipitate in pure water with low ionic strength!). The columns are sterile packed, pre-swollen and ready-to-use. Removes up to 99.999% salts, dyes, haptens and other small molecules. Samples up to 100µL are processed in under 5 minutes!

Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments We use magnetic beads with a ferrimagnetic core and an agarose coating coupled to a ligand of choice, our beads meet our own high quality standards. They enable fast and easy purification steps, which can be automated. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. The rho1D4 purification system is based on the highly specific binding of the rho1D4 antibody to the rho1D4 epitope fused to proteins. This system has proven particularly effective with membrane proteins. Magnetic beads are useful for protein purification of dilute samples, and for pull-down experiments to determine protein-protein interactions. Elution of the protein can be done the rho1D4 peptide, which competitively binds to the rho1D4 antibody, thereby release the traget protein. The peptide can be purchased individually, or together with Genaxxon Rho1D4 MagBeads as part of a Starter Set. A 5% suspension is provided that binds up to 3mg of protein per mL settled beads. Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

Set of Rho1D4 agarose plus the Rho1D4 peptide for elution. Proteins tagged with the epitope sequence of the Rho1D4 antibody can be purified with this affinity matrix. Rho1D4 peptide in the Elution Buffer serves to competitively bind to the affinity resin and release the tagged protein, providing gentler elution conditions than, for example, changing pH. Genaxon bioscience offers conveniently aliquoted Rho1D4 peptide that has been tested with Rho1D4 Agarose. Based on BioWorks Workbeads, this product is the first commercially available immunoaffinity resin for the Rho1D4 purification system. Properties:- demonstrably suitable for membrane protein research - binding capacity of 3-4mg protein per mL resin - purifies protein with high specificity - gentle protein elution based on competitive binding - can be regenerated for reuse. Genaxxon resins are produced under strict quality guidelines and each batch undergoes quality checks to ensure that the loaded matrix has a high protein capacity. Combined with the specificity of the antibody-epitope interaction, a purification protocol optimized for the target protein can generate elution fractions with exceptionally high yields. This set offers both the Rho1D4 agarose plus the Rho1D4 peptide for elution.

Proteins tagged with the epitope sequence of the Rho1D4 antibody can be purified with this affinity matrix. Rho1D4 peptide in the Elution Buffer serves to competitively bind to the affinity resin and release the tagged protein, providing gentler elution conditions than, for example, changing pH. Genaxon bioscience offers conveniently aliquoted Rho1D4 peptide that has been tested with Rho1D4 Agarose. Based on BioWorks Workbeads, this product is the first commercially available immunoaffinity resin for the Rho1D4 purification system. Properties: - demonstrably suitable for membrane protein research - binding capacity of 3-4mg protein per mL resin - purifies protein with high specificity - gentle protein elution based on competitive binding - can be regenerated for reuse. Genaxxon resins are produced under strict quality guidelines and each batch undergoes quality checks to ensure that the loaded matrix has a high protein capacity. Combined with the specificity of the antibody-epitope interaction, a purification protocol optimized for the target protein can generate elution fractions with exceptionally high yields. This set offers both the Rho1D4 agarose plus the Rho1D4 peptide for elution. Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

Rho1D4 peptide in the Elution Buffer serves to competitively bind to the affinity resin and release the tagged protein, providing gentler elution conditions than, for example, changing pH. Genaxxon offers conveniently aliquoted Rho1D4 peptide that has been tested with our Rho1D4 Agarose. The peptide can be purchased individually, or together with Genaxxon Rho1D4 Agarose as part of a Starter Set. 5mg peptide is sufficient for 1mL Rho1D4 Agarose or MagBeads. Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

Rho1D4 antibody (antibody to Rhodopsin) is available as a purified mouse monoclonal antibody. The Rho1D4 specifically binds to the C- terminal epitope -T-E-T-S-Q-V-A-P-A- (COOH) of rhodopsin. This epitope can be engineered onto the C-terminus of proteins by recombinant techniques for use in - immunocytochemical analysis of proteins- co-immunoprecipitation- purification of epitope-tagged proteins for structure-function studies- related affinity-based techniques Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

Glutathione MagBeads are developed for the affinity purification of glutathione-S-transferase (GST) fusion proteins. The affinity matrix is based on spherical magnetic cross-linked agarose. The material is highly porous to allow optimal protein interaction. Cross-linked agarose is also physically very stable, making it suitable for purification processes without deformation or destruction. Our magnetic beads are very homogeneous in size with a medium particle diameter of 30µm, yielding a high degree of reproducibility between individual purification runs.Glutathione is coupled to the magnetic agarose beads to obtain an affinity matrix with highest binding capacity for GST fusion proteins. Because the purification method depends on correctly folded GST protein, only native conditions can be used.Glutathione MagBeads are delivered as a 25% suspension in 20% ethanol. Therefore, 1mL suspension will yield a 250µL bed volume. The suspension contains 20% ethanol to prevent microbial growth. Genaxxon MagBeads have a binding capacity of >10mg protein/mL settled beads. Protein Binding CapacityThe protein binding capacity is up to 10mg/mL settled beads, as determined by purification of glutathione-S-transferase (GST) from E.coli cleared lysates, and quantified via spectrophotometry. GST-tagged proteins expressed in bacteria, yeasts, insects and in cell culture supernatants can be readily purified in a single purification step even from proteins expressed at low levels. After binding of target molecules, the magnetic resin can be readily isolated with the aid of a magnet. The GST tag can be cleaved in bound condition or in eluted condition by specific proteases such as TEV-express. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Genaxxon MagBeads are ferrimagnetic agarose beads coupled to glutathione, an efficient ligand for GST fusion proteins. Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

The GST tag is a 26 kDa protein that binds to glutathione-coupled agarose resins. This tag is considerably larger than the His tag and is often chosen to promote recombinant protein folding. In a comparative study with equivalent, market-leading resins, Geanxxon's Glutathione Agarose proved to be a competitive alternative, exhibiting the same protein binding capacity. Compared to competitor G both resins yielded up to 10mg/mL protein. Protein binding capacity: up to 10mg/mL. Compatible with a range of detergents and additives.

The Medicago Protein L-Ligand Leakage Elisa kit has been designed to detect and quantify Protein L in Immunoglobulin (Ig) or Ig-fragment containing solutions, e.g. eluates from Protein L affinity chromatography. The kit was developed as a complement to Cytiva’s affinity chromatography medium Capto L.Easy, stepwise procedureThe Protein L Ligand Leakage Elisa kit is easy to use. It contains all the components necessary for your daily lab routine and is based on a simple stepwise procedure. The concentration of Protein L in your samples is calculated and presented as a calibration curve indicating any ligand leakage in your eluates.Verification of pharmaceutical processesThe kit is for verifying affinity chromatography process quality in purification of pharmaceuticals using chromatography. A low leakage level of Protein L in the column used is proof that both the medium and the process as such are performing as expected. Compared to developing your own protocols, using this validated detection kit is an easy and secure way for researchers and manufacturers to continuously check for leakage and to ensure process quality.The kit comes as a sandwich ELISA with microtiter strips coated with an affinity purified anti-Protein L IgY-antibody. In this assay, samples are boiled to separate the Ig/Ig-fragments from the Protein L in the solution, centrifuged, and incubated in the strip wells. The bound Protein L is then detected by adding a horseradish peroxidase (HRP)-conjugated anti-Protein L IgY antibody. Substrate is added to induce a color change which is proportional to the amount of bound Protein L from the sample. The color change is quantified spectrophotometrically at 450nm by using a plate reader. The amount of Protein L in the sample is determined by using a standard curve prepared in a reference sample. The reference sample should have the same concentration of Ig or Ig-fragments and preferably the same buffer composition as the elution samples from the Protein L affinity chromatography medium. However, it should be prepared using another purification strategy, e.g. ion exchange chromatography. Once a reference sample is created it can be aliquoted and used for several different protein L ELISA assays. ApplicationsDetermination of leakage levels of Protein L in Protein L-medium purification of intact immunoglobulin or immunoglobulin fragments (Ig or Ig fragments). Some fab samples may require optimization. Currently there is no protocol available for sdab molecules. Shipping and storageThe product is shipped cooled (+2°C to +8°C) except the Protein L reference which is shipped frozen (-20°C). On arrival store components cooled ( +2°C to +8°C ) except the Protein L reference which should be stored at -20/-80°C.Strips (96 wells): Store unused strips in sealed bag at +2°C to +8°C . HRP-conjugated anti Protein L pAb and EC-Blue Enhanced TMB-substrate: Store protected from light. Protein L reference: Store in working aliquots to avoid repeated freezing/thawing.

This ELISA-kit has been designed to detect and quantify Protein L in Immunoglobulin (Ig) or Ig-fragment containing solutions. It is a sandwich ELISA with microtiter strips coated with an affinity purified anti-Protein L IgY-antibody. In this assay, samples are boiled to separate the Ig/Ig-fragments from the Protein L in the solution, centrifuged, and incubated in the strip wells. The bound Protein L is then detected by adding a horseradish peroxidase (HRP)-conjugated anti-Protein L IgY antibody. Substrate is added to induce a color change which is proportional to the amount of bound Protein L from the sample. The color change is quantified spectrophotometrically at 450nm by using a plate reader. The amount of protein L in the sample is determined by using a standard curve prepared in a reference sample. The reference sample should have the same concentration of Ig or Ig-fragments and preferably the same buffer composition as the elution samples from the Protein L affinity chromatography medium. However, it should be prepared using another purification strategy, e.g. ion exchange chromatography. Once a reference sample is created it can be aliquoted and used for several different protein L ELISA assays. Applications: Determination of leakage levels of protein L in protein L-medium purification of intact immunoglobulin or immunoglobulin fragments (Ig or Ig fragments). Some fab samples may require optimization. Currently there is no protocol available for sdab molecules. Shipping and storage. The product is shipped cooled (+4°C) except the protein L reference which is shipped frozen (-20°C). On arrival store components cooled (+4ºC) except the protein L reference which should be stored at -20/-80°C: Strips (96 wells), store unused strips in sealed bag at +4ºC. HRP-conjugated anti Protein L pAb and EC-Blue Enhanced TMB-substrate, store protected from light. Protein L reference,store in working aliquots to avoid repeated freezing/thawing.

Fast DNA Extraction & Hot-Start PCR KitThe DNA Q-Extraction Hot-Start PCR Kit combines our rapid DNA Q-Extraction Solution with the high-performance SuperHot Mastermix (2X) Blue. This all-in-one kit enables fast DNA extraction and reliable Hot-Start PCR – with PCR-ready DNA in just 8 minutes, without any additional purification steps. Ideal for genotyping and the amplification of DNA from a wide range of sources including mammalian tissues, bacterial cells, saliva, and oral swabs.Recent user data confirm that the DNA Q-Extraction Solution can also be successfully used with fungi samples, such as yeast and mold. The standard 8-minute protocol yields PCR-ready DNA without the need for additional clean-up or purification steps. This expands the range of applications and makes the DNA Q-Extraction Solution a versatile tool for rapid DNA extraction, even from more challenging sample types.Key FeaturesCombined DNA extraction & hot-start PCRFast 8-minute protocolNo centrifugation or dilution neededPCR-ready DNA from cells and tissuesIncludes loading dye for direct gel loading and pipetting controlApplicationThe simple one-step lysis is performed in two heating steps (65 °C / 98 °C) using a thermocycler or heating block. The resulting lysate contains PCR-ready DNA, suitable for standard and real-time PCR, without vortexing, centrifugation, or dilution.The included SuperHot Mastermix (2X) Blue contains a hot-start Taq polymerase for improved specificity and sensitivity. The integrated blue loading dye allows direct gel loading and visual control of pipetting.Kit ComponentsDNA Q-Extraction SolutionSuperHot Mastermix (2X) BlueStorageExtracted DNA is stable at -20 °C for up to one week, and for long-term storage at -80 °C.Also available as a non-hot-start version with our reliable Red Mastermix (2X) – perfect for robust results in routine PCR.Application Notes:application note genotyping with m3231_dna_q_extraction_solution_en_vs250527

Fast DNA Extraction & PCR in One KitThe DNA Q-Extraction PCR Kit Red combines the powerful DNA Q-Extraction Solution with our reliable Red Mastermix (2X) – enabling rapid and easy DNA extraction followed by PCR amplification in a single streamlined workflow. Perfect for genotyping, this kit delivers PCR-ready DNA in just 8 minutes from a variety of sample types such as mammalian cells, bacteria, saliva, buccal swabs, or tissue samples.Recent user data confirm that the DNA Q-Extraction Solution can also be successfully used with fungi samples, such as yeast and mold. The standard 8-minute protocol yields PCR-ready DNA without the need for additional clean-up or purification steps. This expands the range of applications and makes the DNA Q-Extraction Solution a versatile tool for rapid DNA extraction, even from more challenging sample types.Your Benefits at a GlanceFast 8-minute protocolNo vortexing, centrifugation or dilutionPCR-ready DNA directly from lysateSimple protocol: lysis and PCR with minimal handlingNon-toxic reagents – safe and convenientScalable and automation-friendlyDirect gel loading thanks to built-in loading dyeApplicationThe lysis is performed in two simple heating steps (65 °C for 6 min, 98 °C for 2 min) using a thermocycler or heating block. The resulting DNA is immediately ready for PCR without any further purification – ideal for genotyping applications. The included Red Mastermix (2x) ensures reliable amplification results and enables direct loading onto agarose gels with visual pipetting control.Kit ComponentsDNA Q-Extraction SolutionRed Mastermix (2X)Storage & StabilityExtracted DNA is stable at -20 °C for up to one week. For long-term storage, we recommend -80 °C.Also available as a hot-start version with our SuperHot Mastermix (2X) Blue – ideal for increased specificity with complex templates.Application Notes:application note genotyping with m3231_dna_q_extraction_solution_en_vs250527

The non-toxic Plant DNA Q-Extraction Solution provides fast and easy extraction of nucleic acids from various plant material (e.g. ivy or stinging nettle). The PCR-ready nucleic acid is extracted, using one tube and one reaction, in as little as 8 minutes. The one-step lysis is performed in either a thermocycler or heating block and is divided into two simple heating steps. The extracted DNA is then ready for PCR without further handling such as vortex, centrifugation or dilutions. The obtained DNA extract is stable at -20°C for up to one week and at -80°C for long term storage. For optimal genotyping results we recommend to use our Plant DNA Q-Extraction Solution together with our Red Mastermix Master Mix (2X) for PCR (M3029) or our SuperHot Mastermix Blue (2X) (M2007b). E.g. use 2µL to 5µL of the Plant DNA Q-Extraction solution for a 25µL PCR reaction. Features:- One-reagent set-up- Minimal handling- Rapid 8-minute protocol- PCR-ready DNA- no sample / yield loss- DNA extracts from plants- Non-toxic reagents- Scalable set-up- Automation-friendly Extraction ProtocolPreparation of DNA extraction should be performed in a separate area from that used for setting up the PCR reaction. 1. Thaw Plant DNA Q-Extraction Solution. For the first time use, aliquot the Plant DNA Q-Extraction solution into smaller volumes. (Plant DNA Q-Extraction Solution has a cloudy appearance).2. Add your sample to a tube containing 100µL Plant DNA Q-Extraction Solution. Recommended sample sizes are shown in the table of the data sheet.3. Vortex the tube containing the sample and the DNA extraction solution for 15 sec. Make sure that the sample is completely covered by the Plant DNA Q-Extraction Solution.4. Transfer the tube to a heat block or a thermal cycler and incubate for 1. 65 °C for 6 min2. 98 °C for 2 min3. 4 °C (or cool down on ice) The DNA extract is now ready for PCR. Applications examples:Efficient Amplification of DNA extracts from different plant tissue (leaves).Plant DNA Q-Extraction Solution was used together with our RedMastermix (M3029) to extract and amplify genomic DNA from different plant tissues. For DNA extraction, 10mg tissue (leave) was added to 100µL of DNA Q-Extraction Solution.Figure 1: DNA extracts of stinging needle and ivy. PCR tubes in duplicate with 100µL DNA Q Extract Solution + 10mg leave material from either stinging neetles or ivy. A. stinging neetle and B. Ivy before extraction with DNA Q Extraction Solution. C. stinging neetle and D. Ivy after extraction with DNA Q Extraction Solution.Figure 2. The extracted DNA from ivy was amplified using RedTaq MasterMix (M3029) and two primer sets targeting: A. Genomic plant DNA (ITS 335 bp) or B. Chloroplast DNA (trnL 380 bp). The positive controls are 1 ng/reaction of DNA purified from stinging nettle using DNeasy Plant Mini Kit (Qiagen). Also included are duplicates of samples extracted in buffer without lysing agents, but using the Q-Extraction protocol (No treatment). All DNA extracts are amplified in duplicates. DNA marker is Iqon PCR Ladder. Application Notes:application note genotyping with m3231_dna_q_extraction_solution_en_vs250527application note realtime pcr with m3230 plant dna_q_extraction_solution_en_vs250527

Reliable RNA Stabilization for Optimal AnalysisThe Genaxxon Fix-it - Nucleic Acid Stabilization Reagent is a non-toxic reagent designed to stabilize and preserve RNA & DNA in biological samples such as tissues, cells, and blood. It rapidly penetrates the sample and effectively prevents DNA and RNA degradation by inhibiting Nuclease activity. This eliminates the need for immediate sample processing or storage in liquid nitrogen. Tissue samples can be collected and immersed in the Fix-it - Nucleic Acid Stabilization Reagent immediately after harvesting, ensuring high RNA quality and yield for subsequent isolation and analysis.AdvantagesReliable RNA stabilization – Protects RNA in tissues, cells, and blood by inhibiting RNases.Immediate protection – Quickly preserves RNA integrity after sample collection.Flexible storage – No need for immediate processing or freezing in liquid nitrogen.High-quality RNA – Ensures optimal yield for downstream applications.Safe and easy to use – Non-toxic, aqueous formulation for convenient handling.Highly effective - Stabilizes RNA for 1 day at 37°C, for 1 week at 25°C, for 1 month at +2°C to +8°C, or indefinitely at -20°C/-80°C.ApplicationsGene expression analysis – Provides high-quality RNA for RT-PCR and qPCR.RNA sequencing (RNA-Seq) – Preserves RNA integrity for transcriptome studies.Microarray analysis – Maintains RNA stability for accurate expression profiling.Biobanking – Enables long-term RNA storage without degradation.Field sample collection – Allows RNA preservation without immediate freezing.

Plasmid DNA purification columns with cap for purification of Plasmid-DNA. -  These columns are a convenient and cheap alternative if buffers of already existing DNA-purification kits are left but columns are out. - The Genaxxon columns can also be used for left-over buffer from purification kits of other suppliers than Genaxxon. Content: 50 columns.

Genaxxon's Exosome Protein Extraction Kit is especially engineered to extract proteins from various exosomes with high efficiency. The kit is the perfect solution for researchers aiming for high-quality Western Blot results.Key Features: High Protein Yield: Achieve a high concentration of exosome proteins, ensuring optimal results for your Western Blot experiments. Effective Lysis: The kit contains a special protein denaturant that effectively lyses exosomes, maximizing protein extraction. Easy to Use: Simply mix the kit with your exosome solution to lyse the exosomes. The lysate can then be directly loaded into SDS-PAGE for straightforward analysis. Accurate Quantification: Measure the concentration of exosome proteins in the lysates using either the Bradford or BCA protein concentration assay methods. Western Blot Ready: The denatured proteins are perfectly suited for Western Blot applications, ensuring reliable and high-quality results. Note: These proteins are not suitable for ELISA or enzyme activity measurements. Applications: Western Blot Analysis: Ideal for researchers focused on obtaining high-quality Western Blot data. Protein Quantification: Compatible with Bradford and BCA assay methods for accurate protein concentration measurement. Choose the Genaxxon Exosome Protein Extraction Kit for a reliable, efficient, and high-yield extraction of exosome proteins, tailored specifically for Western Blot success. 

Genaxxon’s Exosome Maxi Purification Kit ensures high efficiency in exosome and extracellular vesicle (EV) isolation. Designed to purify exosomes from cell culture medium, this kit leverages advanced magnetic bead technology. Achieve results comparable to or better than ultracentrifugation, without the need for specialized equipment. The kit efficiently isolates exosomes using magnetic beads that adsorb exosomes during purification and release them in the elution buffer, ensuring high purity and yield. These eluted exosomes are ideal for cell culture applications and animal injections and are suitable for immediate functional analyses such as NTA. Applications The Genaxxon Serum Exosome Purification Kit is versatile and can be used to isolate microvesicles from cell culture medium. The isolated vesicles are perfect for a range of analytical and functional applications, including: Physical Characterization: Techniques such as Nanoparticle Tracking Analysis (NTA) and Tunable Resistive Pulse Sensing (TRPS) Electron MicroscopyIsolation and Analysis: Suitable for DNA, RNA, proteins, lipids, and other constituentsFlow CytometryAntibody- or Fluorescent Dye-Based LabelingUptake by Recipient Cells For smaller sample volumes, the kit allows for volume adjustment using ddH2O, maintaining flexibility and efficiency in your experimental setups. Choose the Genaxxon Exosome Maxi Purification Kit for reliable, high-quality exosome isolation, enabling advanced research and application in various biological fields.Genaxxon also offers a Exosome Protein Purification Kit (S5328), and other Exosome purification kits for your specific needs (Exosome Mini Purification Kit (S5321) for small-volume samples or the Serum Exosome Purification Kit (S5323)).

Genaxxon’s Serum Exosome Purification Kit ensures high efficiency in exosome and extracellular vesicle (EV) isolation. Designed to purify exosomes from serum and blood plasma, this kit leverages advanced magnetic bead technology. Achieve results comparable to or better than ultracentrifugation, without the need for specialized equipment. The kit efficiently isolates exosomes using magnetic beads that adsorb exosomes during purification and release them in the elution buffer, ensuring high purity and yield. These eluted exosomes are ideal for cell culture applications and animal injections and are suitable for immediate functional analyses such as NTA. Applications The Genaxxon Serum Exosome Purification Kit is versatile and can be used to isolate microvesicles from serum, plasma, or other biological liquid samples. The isolated vesicles are perfect for a range of analytical and functional applications, including: Physical Characterization: Techniques such as Nanoparticle Tracking Analysis (NTA) and Tunable Resistive Pulse Sensing (TRPS) Electron MicroscopyIsolation and Analysis: Suitable for DNA, RNA, proteins, lipids, and other constituentsFlow CytometryAntibody- or Fluorescent Dye-Based LabelingUptake by Recipient Cells The exosome purification system provided in this kit is capable of purifying 20 ml of serum, plasma, or other highly viscous samples, meeting various experimental requirements such as exosomal protein extraction, RNA extraction, and miRNA extraction. This kit is not suitable for extracting exosomes from highly concentrated cell culture medium. Choose the Genaxxon Serum Exosome Purification Kit for reliable, high-quality exosome isolation, enabling advanced research and application in various biological fields.Genaxxon also offers a Exosome Protein Purification Kit (S5328), and other Exosome purification kits for your specific needs (Exosome Mini Purification Kit (S5321) for small-volume samples or the best selling product Exosome Maxi Purification Kit (S5326).

Genaxxon’s Exosome Mini Purification Kit ensures high efficiency in exosome and extracellular vesicle (EV) isolation. Designed to purify exosomes from small volumes (0.69mL per sample) of major biofluids such as serum, blood plasma, and other exosome-rich samples, this kit leverages advanced magnetic bead technology. Achieve results comparable to or better than ultracentrifugation, without the need for specialized equipment. The kit efficiently isolates exosomes using magnetic beads that adsorb exosomes during purification and release them in the elution buffer, ensuring high purity and yield. These eluted exosomes are ideal for cell culture applications and animal injections and are suitable for immediate functional analyses such as NTA.Features and Benefits • Short purification time (2h) and good integrity of purified exosomes• Not contain other compounds and used directly in cell culture experiments and animal in vivo injection experiments• High purity and low levels of exogenous contaminant proteins• Simple and efficient, suitable for processing large quantities of samplesApplications The Genaxxon Exosome Mini Purification Kit is versatile and can be used to isolate microvesicles from serum, plasma, cell culture supernatant or other biological liquid samples. The isolated vesicles are perfect for a range of analytical and functional applications, including: Physical Characterization: Techniques such as Nanoparticle Tracking Analysis (NTA) and Tunable Resistive Pulse Sensing (TRPS) Electron MicroscopyIsolation and Analysis: Suitable for DNA, RNA, proteins, lipids, and other constituentsFlow CytometryAntibody- or Fluorescent Dye-Based LabelingUptake by Recipient Cells For smaller sample volumes, the kit allows for volume adjustment using ddH2O, maintaining flexibility and efficiency in your experimental setups. Choose the Genaxxon Exosome Mini Purification Kit for reliable, high-quality exosome isolation, enabling advanced research and application in various biological fields.Genaxxon also offers a Exosome Protein Purification Kit (S5328 >), and other Exosome purification kits for your specific needs (Serum Exosome Purification Kit (S5323 >) or the best selling product Exosome Maxi Purification Kit (S5326 >).

The Genomic DNA Purification Kit - Tissue is specially designed for fast and convenient isolation of highest quality genomic DNA from animal tissue. Yield: up to 50µg DNA. Yields are depending on applied material. Genaxxon's technology guarantees easy handling, low hands-on time, extremely high yields and high quality standards. The high binding capacities of Genaxxon matrices allow the passage of even highly viscous extracts without retention and the isolation of unmet quantities of DNA. This kit DOES NOT include the enzymes Proteinase K and RNase A. Genaxxon also offers the kit with the enzymes (GS5378).Other DNA purification kits for the isolation of DNA from different sample types, tissue and organismns are also available from Genaxxon. GS5380 > (PCR and Gel extraction Mini Prep Kit) - GS5369 > (Plasmid DNA Mini Purification Kit) or GS5370 > (Genomic DNA Purification Kit - Blood/Cell culture). You can also buy separate PCR DNA purification columns (TS5313 >) which can be used for Genaxxon´s kits or for these of competitors!

The Genomic DNA Purification Kit - Tissue is specially designed for fast and convenient isolation of highest quality genomic DNA from animal tissue. Yield: up to 50µg DNA. Yields are depending on applied material. Genaxxon's technology guarantees easy handling, low hands-on time, extremely high yields and high quality standards. The high binding capacities of Genaxxon matrices allow the passage of even highly viscous extracts without retention and the isolation of unmet quantities of DNA. This kit includes the enzymes Proteinase K and RNase A. Genaxxon also offers the kit without the enzymes (GS5379).Other DNA purification kits for the isolation of DNA from different sample types, tissue and organismns are also available from Genaxxon. GS5380 > (PCR and Gel extraction Mini Prep Kit) - GS5369 > (Plasmid DNA Mini Purification Kit) or GS5370 > (Genomic DNA Purification Kit - Blood/Cell culture). You can also buy separate PCR DNA purification columns (TS5313 >) which can be used for Genaxxon´s kits or for these of competitors!

Genaxxon´s Total RNA Purification Kit - Whole Blood/Cell culture is designed for the rapid and efficient purification of high quality RNA from whole blood (up to 300µL) or mammalian/bacterial/fungi cells.  The isolation protocol and buffer formulations were optimised for high isolation efficiency and purity of RNA. The Total RNA Purification Kit utilises spin columns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. The isolation procedure consists of 5 steps. In the first step, cells are harvested and then lysed in the second step. Any RNases are inactivated. The two-step washing stage effectively removes impurities and enzyme inhibitors. The purified RNA is eluted using a low ionic strength buffer or RNase-free water and can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR and so forth. Genaxxon offers also ceramic beads for rapid cell disintegration and other RNA purification kits. With our HotScriptase RT Mastermix, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step.