RedMasterMix (2x) - PCR MasterMix with red dye to help visualize pipetting and mixing steps. Electrophoresis can be performed immediately after PCR without the need of a gel loading buffer. This makes this MasterMix time efficient, cost efficient and a reliable choice for the best PCR results with high efficiency.  One tube, one pipetting step. RedMasterMix (2x) is delivered to you as a optimized, ready-to-use solution containing- Taq DNA Polymerase - dNTPs- MgCl2- a red dye- reaction bufferfor efficient amplification of DNA. Additionally the RedMasterMix (2x) contains an additive and a red dye for proceeding with electrophoresis after PCR without adding loading buffer. Just add your primers and template DNA. This PCR MasterMix (2x) has been optimized for use in routine PCR amplification of DNA templates in the range of 0.2-4kb. The special formulation of our PCR RedMasterMix (2x) can withstand repeated freezing/thawing without compromising yields, sensitivity or results, when used as directed. The PCR MasterMix with red dye is efficient (no left over of reagents), scalable from 10µL to 50µL and stable for at least 24 months.Our RedMastermix can also be used for Sanger sequencing. Just dilute the PCR reaction 1:8 after PCR, or purify using spin columns and then apply. Read now in our blog how Genaxxon's RedMasterMix can also simplify your laboratory work. Our Agaroses > and DNA Ladders > are ideally suited for subsequent electrophoresis of PCR products.

Highly pure, HPLC purified (>99%) dNTPs packaged as 4 separate 100mM solutions of dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. Genaxxon’s dNTP solutions have been optimized for use in DNA amplification and other related methods. Genaxxon dNTPs contain no measurable bacterial or human DNA. For long term storage and/or for repeated use, our recommendation is to aliquot the stock solutions. Solutions of dNTP sodium salts (dATP, dCTP, dGTP und dTTP) Concentration: 100 mM each nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mixes with 2 mM or 10 mM or the modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003) as well as the ready-to-use RedMastermix (M3029), already including dNTPs.

Overview:Proteinase K is a robust and versatile serine protease used extensively in molecular biology for the digestion of proteins in nucleic acid preparations. This solution, at a concentration of 20 mg/mL, is ideal for a wide range of applications including the preparation of DNA and RNA samples. The PCR grade quality ensures that the enzyme is free from contaminants that could interfere with downstream applications, making it highly suitable for sensitive techniques such as PCR.Product Details:ready-to-useConcentration: 20 mg/mLactive over a wide range of reaction conditionsAn elevation of the reaction temperature from 37°C to 50 - 60°C may increase the activity several timesQuality: PCR grade, ensuring high purity and no interference with PCR reactions.Applications: Suitable for DNA and RNA extractionRemoval of protein contaminantsPreparation of samples for various molecular biology techniques.Benefits:High Purity: The PCR grade quality guarantees minimal contamination, ensuring reliable results in sensitive applications. Versatility: Ideal for various molecular biology procedures, including sample preparation for PCR.Convenience: Ready-to-use solution at an optimal concentration for ease of use. Notes:The recommended working concentration of Proteinase K is 0.05 to 1 mg/mL. The activity of the enzyme is stimulated by 0.2 to 1% SDS and also by 1 to 4M ureaCa2+ protects Proteinase K against autolysis and increases the thermal stabilityStable over a wide pH range: 4.0 to 12.5, optimum pH 7.5 to 8.0Activity optimum: 50°C to 55°CRapid denaturation of enzyme occurs at temperatures above 65°CFeatures:No detected exonuclease, endonuclease or RNase activitySpecific activity: >40 units/mg protein Activity: ≥30 units/mg lyophilizateSolubility: ≥20 mg/mLDNA content ≤10 pg/mgShipping conditions: Shipment on wet iceInhibitors of proteinase KWhich substances can inhibit proteinase K? Proteinase K can be inhibited by a variety of substances. These include ‘denaturing substances’, detergents and proteinase inhibitors. Sensitivity to inhibitors depends pretty much on the specific application (exact pH, concentration, temperature). Inhibitors frequently used in biochemistry and molecular biology are:SDS: High concentrations of SDS can denature and inactivate proteinase K.EDTA: EDTA is a chelating agent that can bind to metal ions that are essential for proteinase K activity.Urea: High concentrations of urea can denature and thereby inactivate proteinase K.Detergents: Some detergents, such as Triton X-100 or Tween® 20, can inhibit proteinase K, especially when the detergents are present in high concentrations.Protease inhibitors: Protease inhibitors, such as phenylmethylsulfonyl fluoride (PMSF), can irreversibly inhibit proteinase K.Chaotropic salts: Depending on the DNA extraction protocol, the presence of chaotropic salts or detergents may increase proteinase K activity. However, the same reagents may also inhibit proteinase K activity.Proteinase K can be obtained from Genaxxon as Powder (M3036) > or as 20mg/mL Solution (M3037) >.Produced by Genaxxon bioscience GmbH, founded in 2002 by Dr. Norbert Tröndle to provide reliable products for PCR and custom cell culture media formulations, the Proteinase K Solution 20 mg/mL offers a dependable and efficient solution for your molecular biology needs. Achieve consistent, high-quality results with an enzyme designed specifically for your requirements.

Why Choose Challenger Hotstart RT-qPCR Mix?Room-temperature setup – Easy pipetting without cooling, saving time and reducing errors.warm-start reverse transcriptase – Prevents nonspecific amplification and allows primer/probe premixing.Superior contamination control – UDG/dUTP system eliminates carryover contamination for reliable qPCR results.Fast RT-qPCR protocols – Complete assays in just 30 minutes with a 5-minute reverse transcription step.High sensitivity RT-qPCR – Detect as few as 1–10 RNA copies.Flexible applications – Single and multiplex RT-qPCR for viral detection, gene expression, and diagnostics.Optimized for High-Performance qPCR WorkflowsThe 4× concentrated formulation allows high-throughput and multiplexing while keeping reaction efficiency high. The combination of warm-start RTase and antibody-modified Taq DNA polymerase and a RNAse inhibitor ensures precise control, so your reactions only start when you are ready.Contamination-Free One-Step RT-qPCRBuilt-in dUTP/UDG safeguards protect your assays from false positives due to carryover contamination. This, in combination with an RNase inhibitor is essential for labs running high-sensitivity viral RNA detection and diagnostic assay developments.Fast RT-qPCR Protocols Without Sacrificing AccuracyWith Challenger, you can speed up detection while keeping sensitivity intact:Standard program: 55 °C for 15 min RT, ~1 hr totalFast program: 55 °C for 5 min RT, ~30 min total Benchmark studies with HBV/HCV RNA samples confirmed superior stability and consistent Ct values compared to competitor brands.Long-Term Stability You Can TrustUnlike conventional RT-qPCR mixes, the Challenger Hotstart RT-qPCR Master Mix is engineered for long shelf life. Even under accelerated testing at 37 °C for 10 days, the mix maintained stable multiplex detection - ensuring reliable performance during storage and shipping.Example ApplicationsViral RNA detection: HBV, HCV, Influenza, RSV, SARS-CoV-2, hMPV, PIV1, and moreGene expression analysis: accurate detection of high- and low-copy RNA targetsClinical research & diagnostics: robust, reproducible dataHigh-throughput testing: multiplex RT-qPCR panels for respiratory, gastrointestinal, and hepatitis virusesMany more…Key Features SummaryOne-step RT-qPCR Master Mix with warm-start RTaseHigh sensitivity qPCR mix – detects single RNA copiesFast RT-qPCR protocol – 5 min RT, 30 min totalLong stability RT enzyme – stable at 37 °C for 10 daysUDG/dUTP contamination control – eliminates carryover risk4× concentrated format – supports multiplexing High sensitivity and stable detectionStable detection of single-digit copy templates using the Challenger Hotstart RT-qPCR 4X Mastermix:

Direct PCR from Plant Samples – Fast, Robust and Inhibitor-ResistantThe InhibiCore Rapid Plant DNA Q-Extraction PCR Kit is a streamlined, high-performance solution for rapid DNA analysis from plant material. It combines the efficient Plant DNA Q-Extraction Solution (M3230) with the highly robust InhibiCore Rapid Plant PCR MasterMix (5X) (M3250), enabling a complete workflow from sample preparation to reliable qPCR analysis.Plant tissues often contain strong PCR inhibitors such as polyphenols, polysaccharides, and other secondary metabolites. This kit is specifically designed to overcome these challenges, delivering reproducible and highly sensitive qPCR results even from difficult plant matrices.The Plant DNA Q-Extraction Solution enables fast and efficient DNA release without the need for conventional, time-consuming extraction procedures. Combined with the inhibitor-resistant PCR master mix, it creates a seamless Direct-PCR workflow that significantly reduces hands-on time and simplifies the overall process.The included InhibiCore Rapid Plant PCR MasterMix (5X) is based on a modified HotStart Taq DNA polymerase, ensuring high specificity, sensitivity, and robustness. Its exceptional inhibitor tolerance makes it ideally suited for demanding applications in plant research, agriculture, and quality control. The kit is also lyo-ready and includes dUTP for optional carry-over contamination control when used with UDG (Uracil-DNA Glycosylase). The HotStart polymerase enables convenient room-temperature setup without cooling or activation steps.Key BenefitsComplete solution for fast direct PCR from plant materialSeamless workflow: DNA extraction + qPCR in one systemHigh inhibitor tolerance for robust amplification from plant samplesFast and simple sample preparation without classical DNA isolationHigh sensitivity and specificity for reliable resultsTypical Applications Plant genotyping GMO and transgene detection Agricultural and plant research Plant pathogen detection Seed and quality control High-throughput direct PCR from plant material Applications examples:1. Sensitive qPCR analysis of chlorophyll gene targets from wheat lysatesReliable and sensitive detection of chlorophyll gene targets from plant lysates was demonstrated using qPCR with the InhibiCore Rapid Plant PCR MasterMix (5X) and fluorescent dye chemistry. A serial dilution of wheat lysate (10% to 0.001%), prepared with Plant DNA Q-Extraction Solution (M3230) was used to evaluate sensitivity and inhibitor tolerance.Successful amplification was achieved across all dilutions, confirming strong robustness against plant-derived inhibitors even at high lysate concentrations. A single, specific melt curve peak confirmed assay specificity with no non-specific amplification. Samples were prepared using the rapid 8-minute Plant DNA Q-Extraction Solution protocol, enabling fast DNA preparation without conventional purification steps. Figure 1: qPCR data by wheat lysate qPCR with InhibiCore Rapid Plant PCR MasterMix (5X).The figure shows qPCR amplification curves and melting curve analysis of a wheat lysate dilution series (10% to 0.001%) and a no-template control (NTC) using InhibiCore Rapid Plant PCR MasterMix (5X) and 1xGreenDye. Clear, concentration-dependent amplification is observed in the FAM channel, with reduced signal intensity at 10% lysate due to matrix effects. Melting curve analysis reveals a single specific peak for all positive samples, confirming specific target amplification without non-specific products. 2. Benchmark of Direct PCR performance from 10% tomato lysateThe performance of the InhibiCore Rapid Plant PCR MasterMix (5X) was evaluated against three competitor master mixes for qPCR from 10% tomato lysate. Samples were prepared using the Plant DNA Q-Extraction Solution and analyzed by fluorescent dye detection in the FAM channel.The InhibiCore Master Mix showed successful amplification with the earliest Ct values, demonstrating superior efficiency and the highest inhibitor tolerance compared to all tested competitors. Two competitor systems also produced amplification, while two showed strong inhibition with no detectable signals. Specific amplification was confirmed by a single, distinct melt curve peak with no evidence of non-specific products or primer-dimer formation.Figure 2: Plant direct PCR from plant samples Benchmark - inhibitor effect from Tomato lysateDirect PCR was performed using a cycling protocol of 95 °C for 3 min, followed by 40 cycles of 95 °C for 10 sec and 60 °C for 30 sec, with subsequent melt curve analysis. InhibiCore Rapid Plant PCR MasterMix (5X) and Competitor N showed successful amplification, while Competitor T and Competitor Q were strongly inhibited and did not generate reliable amplification curves. InhibiCore Rapid Plant PCR MasterMix (5X) exhibited the earliest Ct value among all tested systems, indicating the highest tolerance to plant-derived PCR inhibitors and superior performance in crude tomato lysates.