Protein Purification

Genaxxon’s Exosome Maxi Purification Kit ensures high efficiency in exosome and extracellular vesicle (EV) isolation. Designed to purify exosomes from cell culture medium, this kit leverages advanced magnetic bead technology. Achieve results comparable to or better than ultracentrifugation, without the need for specialized equipment. The kit efficiently isolates exosomes using magnetic beads that adsorb exosomes during purification and release them in the elution buffer, ensuring high purity and yield. These eluted exosomes are ideal for cell culture applications and animal injections and are suitable for immediate functional analyses such as NTA. Applications The Genaxxon Serum Exosome Purification Kit is versatile and can be used to isolate microvesicles from cell culture medium. The isolated vesicles are perfect for a range of analytical and functional applications, including: Physical Characterization: Techniques such as Nanoparticle Tracking Analysis (NTA) and Tunable Resistive Pulse Sensing (TRPS) Electron MicroscopyIsolation and Analysis: Suitable for DNA, RNA, proteins, lipids, and other constituentsFlow CytometryAntibody- or Fluorescent Dye-Based LabelingUptake by Recipient Cells For smaller sample volumes, the kit allows for volume adjustment using ddH2O, maintaining flexibility and efficiency in your experimental setups. Choose the Genaxxon Exosome Maxi Purification Kit for reliable, high-quality exosome isolation, enabling advanced research and application in various biological fields.Genaxxon also offers a Exosome Protein Purification Kit (S5328), and other Exosome purification kits for your specific needs (Exosome Mini Purification Kit (S5321) for small-volume samples or the Serum Exosome Purification Kit (S5323)).

Genaxxon’s Exosome Mini Purification Kit ensures high efficiency in exosome and extracellular vesicle (EV) isolation. Designed to purify exosomes from small volumes (0.69mL per sample) of major biofluids such as serum, blood plasma, and other exosome-rich samples, this kit leverages advanced magnetic bead technology. Achieve results comparable to or better than ultracentrifugation, without the need for specialized equipment. The kit efficiently isolates exosomes using magnetic beads that adsorb exosomes during purification and release them in the elution buffer, ensuring high purity and yield. These eluted exosomes are ideal for cell culture applications and animal injections and are suitable for immediate functional analyses such as NTA.Features and Benefits • Short purification time (2h) and good integrity of purified exosomes• Not contain other compounds and used directly in cell culture experiments and animal in vivo injection experiments• High purity and low levels of exogenous contaminant proteins• Simple and efficient, suitable for processing large quantities of samplesApplications The Genaxxon Exosome Mini Purification Kit is versatile and can be used to isolate microvesicles from serum, plasma, cell culture supernatant or other biological liquid samples. The isolated vesicles are perfect for a range of analytical and functional applications, including: Physical Characterization: Techniques such as Nanoparticle Tracking Analysis (NTA) and Tunable Resistive Pulse Sensing (TRPS) Electron MicroscopyIsolation and Analysis: Suitable for DNA, RNA, proteins, lipids, and other constituentsFlow CytometryAntibody- or Fluorescent Dye-Based LabelingUptake by Recipient Cells For smaller sample volumes, the kit allows for volume adjustment using ddH2O, maintaining flexibility and efficiency in your experimental setups. Choose the Genaxxon Exosome Mini Purification Kit for reliable, high-quality exosome isolation, enabling advanced research and application in various biological fields.Genaxxon also offers a Exosome Protein Purification Kit (S5328 >), and other Exosome purification kits for your specific needs (Serum Exosome Purification Kit (S5323 >) or the best selling product Exosome Maxi Purification Kit (S5326 >).

Genaxxon’s Serum Exosome Purification Kit ensures high efficiency in exosome and extracellular vesicle (EV) isolation. Designed to purify exosomes from serum and blood plasma, this kit leverages advanced magnetic bead technology. Achieve results comparable to or better than ultracentrifugation, without the need for specialized equipment. The kit efficiently isolates exosomes using magnetic beads that adsorb exosomes during purification and release them in the elution buffer, ensuring high purity and yield. These eluted exosomes are ideal for cell culture applications and animal injections and are suitable for immediate functional analyses such as NTA. Applications The Genaxxon Serum Exosome Purification Kit is versatile and can be used to isolate microvesicles from serum, plasma, or other biological liquid samples. The isolated vesicles are perfect for a range of analytical and functional applications, including: Physical Characterization: Techniques such as Nanoparticle Tracking Analysis (NTA) and Tunable Resistive Pulse Sensing (TRPS) Electron MicroscopyIsolation and Analysis: Suitable for DNA, RNA, proteins, lipids, and other constituentsFlow CytometryAntibody- or Fluorescent Dye-Based LabelingUptake by Recipient Cells For smaller sample volumes, the kit allows for volume adjustment using ddH2O, maintaining flexibility and efficiency in your experimental setups. Choose the Genaxxon Serum Exosome Purification Kit for reliable, high-quality exosome isolation, enabling advanced research and application in various biological fields.Genaxxon also offers a Exosome Protein Purification Kit (S5328), and other Exosome purification kits for your specific needs (Exosome Mini Purification Kit (S5321) for small-volume samples or the best selling product Exosome Maxi Purification Kit (S5326).

Genaxxon's Exosome Protein Extraction Kit is especially engineered to extract proteins from various exosomes with high efficiency. The kit is the perfect solution for researchers aiming for high-quality Western Blot results.Key Features: High Protein Yield: Achieve a high concentration of exosome proteins, ensuring optimal results for your Western Blot experiments. Effective Lysis: The kit contains a special protein denaturant that effectively lyses exosomes, maximizing protein extraction. Easy to Use: Simply mix the kit with your exosome solution to lyse the exosomes. The lysate can then be directly loaded into SDS-PAGE for straightforward analysis. Accurate Quantification: Measure the concentration of exosome proteins in the lysates using either the Bradford or BCA protein concentration assay methods. Western Blot Ready: The denatured proteins are perfectly suited for Western Blot applications, ensuring reliable and high-quality results. Note: These proteins are not suitable for ELISA or enzyme activity measurements. Applications: Western Blot Analysis: Ideal for researchers focused on obtaining high-quality Western Blot data. Protein Quantification: Compatible with Bradford and BCA assay methods for accurate protein concentration measurement. Choose the Genaxxon Exosome Protein Extraction Kit for a reliable, efficient, and high-yield extraction of exosome proteins, tailored specifically for Western Blot success. 

Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments We use magnetic beads with a ferrimagnetic core and an agarose coating coupled to a ligand of choice, our beads meet our own high quality standards. They enable fast and easy purification steps, which can be automated. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Genaxxon MagBeads are ferrimagnetic agarose beads coupled to a chelating ligand (IDA or NTA) coordinating nickel or cobalt ions. All offered ligand-metal systems efficiently bind histidine-tagged proteins. With a binding capacity of up to 40mg protein per mL (Ni-IDA) and 70mg per mL (Ni-NTA) of settled MagBeads, Genaxxon Ni-IDA MagBeads are comparable to equivalent beads from alternative providers.

NTA-Agarose consists of the tetradentate chelating agent, nitrilotriacetic acid (NTA), covalently coupled agarose beads, and is loaded with Co2+ ions. In processing recombinant fusion proteins from eukaryotes or in the case of special protein complexes, Genaxxon Co-NTA Agarose should always be used offering the possibility of higher protein purity levels. Both metal ions feature differing spatial properties and electronic binding potentials. While Co2+ binds only domains adjacent to residual histidine and is thus highly selective for 6×histidine-tagged fusion proteins, Ni2+ ions are less specific and can bind residual histidine outside the polyhistidine tag, which may result in higher contamination with foreign proteins. The unique production process yields a Co-NTA Agarose that exhibits a higher protein binding capacity than that of leading competitor products. Genaxxon Co-NTA Agarose is very robust in the presence of DTT and EDTA. In a stability test, Genaxxon Co-NTA Agarose was exposed to increasing concentrations of DTT or EDTA for 1h. Thereafter, the resins were used to purify E. coli-expressed GFP-His in gravity columns. The binding capacity of the resin decreased in the presence of both DTT and EDTA but the decay rate was shallow. Genaxxon NTA Agarose is supplied as a 50% buffered suspension in 20% Ethanol.

Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments We use magnetic beads with a ferrimagnetic core and an agarose coating coupled to a ligand of choice, our beads meet our own high quality standards. They enable fast and easy purification steps, which can be automated. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Genaxxon MagBeads are ferrimagnetic agarose beads coupled to a chelating ligand (IDA or NTA) coordinating nickel or cobalt ions. All offered ligand-metal systems efficiently bind histidine-tagged proteins. With a binding capacity of up to 40mg protein per mL (Ni-IDA) and 70mg per mL (Ni-NTA) of settled MagBeads, Genaxxon Ni-IDA MagBeads are comparable to equivalent beads from alternative providers.