This ELISA-kit has been designed to detect and quantify Protein L in Immunoglobulin (Ig) or Ig-fragment containing solutions. It is a sandwich ELISA with microtiter strips coated with an affinity purified anti-Protein L IgY-antibody. In this assay, samples are boiled to separate the Ig/Ig-fragments from the Protein L in the solution, centrifuged, and incubated in the strip wells. The bound Protein L is then detected by adding a horseradish peroxidase (HRP)-conjugated anti-Protein L IgY antibody. Substrate is added to induce a color change which is proportional to the amount of bound Protein L from the sample. The color change is quantified spectrophotometrically at 450nm by using a plate reader. The amount of protein L in the sample is determined by using a standard curve prepared in a reference sample. The reference sample should have the same concentration of Ig or Ig-fragments and preferably the same buffer composition as the elution samples from the Protein L affinity chromatography medium. However, it should be prepared using another purification strategy, e.g. ion exchange chromatography. Once a reference sample is created it can be aliquoted and used for several different protein L ELISA assays. Applications: Determination of leakage levels of protein L in protein L-medium purification of intact immunoglobulin or immunoglobulin fragments (Ig or Ig fragments). Some fab samples may require optimization. Currently there is no protocol available for sdab molecules. Shipping and storage. The product is shipped cooled (+4°C) except the protein L reference which is shipped frozen (-20°C). On arrival store components cooled (+4ºC) except the protein L reference which should be stored at -20/-80°C: Strips (96 wells), store unused strips in sealed bag at +4ºC. HRP-conjugated anti Protein L pAb and EC-Blue Enhanced TMB-substrate, store protected from light. Protein L reference,store in working aliquots to avoid repeated freezing/thawing.

The Medicago Protein L-Ligand Leakage Elisa kit has been designed to detect and quantify Protein L in Immunoglobulin (Ig) or Ig-fragment containing solutions, e.g. eluates from Protein L affinity chromatography. The kit was developed as a complement to Cytiva’s affinity chromatography medium Capto L.Easy, stepwise procedureThe Protein L Ligand Leakage Elisa kit is easy to use. It contains all the components necessary for your daily lab routine and is based on a simple stepwise procedure. The concentration of Protein L in your samples is calculated and presented as a calibration curve indicating any ligand leakage in your eluates.Verification of pharmaceutical processesThe kit is for verifying affinity chromatography process quality in purification of pharmaceuticals using chromatography. A low leakage level of Protein L in the column used is proof that both the medium and the process as such are performing as expected. Compared to developing your own protocols, using this validated detection kit is an easy and secure way for researchers and manufacturers to continuously check for leakage and to ensure process quality.The kit comes as a sandwich ELISA with microtiter strips coated with an affinity purified anti-Protein L IgY-antibody. In this assay, samples are boiled to separate the Ig/Ig-fragments from the Protein L in the solution, centrifuged, and incubated in the strip wells. The bound Protein L is then detected by adding a horseradish peroxidase (HRP)-conjugated anti-Protein L IgY antibody. Substrate is added to induce a color change which is proportional to the amount of bound Protein L from the sample. The color change is quantified spectrophotometrically at 450nm by using a plate reader. The amount of Protein L in the sample is determined by using a standard curve prepared in a reference sample. The reference sample should have the same concentration of Ig or Ig-fragments and preferably the same buffer composition as the elution samples from the Protein L affinity chromatography medium. However, it should be prepared using another purification strategy, e.g. ion exchange chromatography. Once a reference sample is created it can be aliquoted and used for several different protein L ELISA assays. ApplicationsDetermination of leakage levels of Protein L in Protein L-medium purification of intact immunoglobulin or immunoglobulin fragments (Ig or Ig fragments). Some fab samples may require optimization. Currently there is no protocol available for sdab molecules. Shipping and storageThe product is shipped cooled (+2°C to +8°C) except the Protein L reference which is shipped frozen (-20°C). On arrival store components cooled ( +2°C to +8°C ) except the Protein L reference which should be stored at -20/-80°C.Strips (96 wells): Store unused strips in sealed bag at +2°C to +8°C . HRP-conjugated anti Protein L pAb and EC-Blue Enhanced TMB-substrate: Store protected from light. Protein L reference: Store in working aliquots to avoid repeated freezing/thawing.

Genaxxon’s Serum Exosome Purification Kit ensures high efficiency in exosome and extracellular vesicle (EV) isolation. Designed to purify exosomes from serum and blood plasma, this kit leverages advanced magnetic bead technology. Achieve results comparable to or better than ultracentrifugation, without the need for specialized equipment. The kit efficiently isolates exosomes using magnetic beads that adsorb exosomes during purification and release them in the elution buffer, ensuring high purity and yield. These eluted exosomes are ideal for cell culture applications and animal injections and are suitable for immediate functional analyses such as NTA. Applications The Genaxxon Serum Exosome Purification Kit is versatile and can be used to isolate microvesicles from serum, plasma, or other biological liquid samples. The isolated vesicles are perfect for a range of analytical and functional applications, including: Physical Characterization: Techniques such as Nanoparticle Tracking Analysis (NTA) and Tunable Resistive Pulse Sensing (TRPS) Electron MicroscopyIsolation and Analysis: Suitable for DNA, RNA, proteins, lipids, and other constituentsFlow CytometryAntibody- or Fluorescent Dye-Based LabelingUptake by Recipient Cells The exosome purification system provided in this kit is capable of purifying 20 ml of serum, plasma, or other highly viscous samples, meeting various experimental requirements such as exosomal protein extraction, RNA extraction, and miRNA extraction. This kit is not suitable for extracting exosomes from highly concentrated cell culture medium. Choose the Genaxxon Serum Exosome Purification Kit for reliable, high-quality exosome isolation, enabling advanced research and application in various biological fields.Genaxxon also offers a Exosome Protein Purification Kit (S5328), and other Exosome purification kits for your specific needs (Exosome Mini Purification Kit (S5321) for small-volume samples or the best selling product Exosome Maxi Purification Kit (S5326).

Genaxxon’s Exosome Mini Purification Kit ensures high efficiency in exosome and extracellular vesicle (EV) isolation. Designed to purify exosomes from small volumes (0.69mL per sample) of major biofluids such as serum, blood plasma, and other exosome-rich samples, this kit leverages advanced magnetic bead technology. Achieve results comparable to or better than ultracentrifugation, without the need for specialized equipment. The kit efficiently isolates exosomes using magnetic beads that adsorb exosomes during purification and release them in the elution buffer, ensuring high purity and yield. These eluted exosomes are ideal for cell culture applications and animal injections and are suitable for immediate functional analyses such as NTA.Features and Benefits • Short purification time (2h) and good integrity of purified exosomes• Not contain other compounds and used directly in cell culture experiments and animal in vivo injection experiments• High purity and low levels of exogenous contaminant proteins• Simple and efficient, suitable for processing large quantities of samplesApplications The Genaxxon Exosome Mini Purification Kit is versatile and can be used to isolate microvesicles from serum, plasma, cell culture supernatant or other biological liquid samples. The isolated vesicles are perfect for a range of analytical and functional applications, including: Physical Characterization: Techniques such as Nanoparticle Tracking Analysis (NTA) and Tunable Resistive Pulse Sensing (TRPS) Electron MicroscopyIsolation and Analysis: Suitable for DNA, RNA, proteins, lipids, and other constituentsFlow CytometryAntibody- or Fluorescent Dye-Based LabelingUptake by Recipient Cells For smaller sample volumes, the kit allows for volume adjustment using ddH2O, maintaining flexibility and efficiency in your experimental setups. Choose the Genaxxon Exosome Mini Purification Kit for reliable, high-quality exosome isolation, enabling advanced research and application in various biological fields.Genaxxon also offers a Exosome Protein Purification Kit (S5328 >), and other Exosome purification kits for your specific needs (Serum Exosome Purification Kit (S5323 >) or the best selling product Exosome Maxi Purification Kit (S5326 >).

Rho1D4 antibody (antibody to Rhodopsin) is available as a purified mouse monoclonal antibody. The Rho1D4 specifically binds to the C- terminal epitope -T-E-T-S-Q-V-A-P-A- (COOH) of rhodopsin. This epitope can be engineered onto the C-terminus of proteins by recombinant techniques for use in - immunocytochemical analysis of proteins- co-immunoprecipitation- purification of epitope-tagged proteins for structure-function studies- related affinity-based techniques Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

Genaxxon’s Exosome Maxi Purification Kit ensures high efficiency in exosome and extracellular vesicle (EV) isolation. Designed to purify exosomes from cell culture medium, this kit leverages advanced magnetic bead technology. Achieve results comparable to or better than ultracentrifugation, without the need for specialized equipment. The kit efficiently isolates exosomes using magnetic beads that adsorb exosomes during purification and release them in the elution buffer, ensuring high purity and yield. These eluted exosomes are ideal for cell culture applications and animal injections and are suitable for immediate functional analyses such as NTA. Applications The Genaxxon Serum Exosome Purification Kit is versatile and can be used to isolate microvesicles from cell culture medium. The isolated vesicles are perfect for a range of analytical and functional applications, including: Physical Characterization: Techniques such as Nanoparticle Tracking Analysis (NTA) and Tunable Resistive Pulse Sensing (TRPS) Electron MicroscopyIsolation and Analysis: Suitable for DNA, RNA, proteins, lipids, and other constituentsFlow CytometryAntibody- or Fluorescent Dye-Based LabelingUptake by Recipient Cells For smaller sample volumes, the kit allows for volume adjustment using ddH2O, maintaining flexibility and efficiency in your experimental setups. Choose the Genaxxon Exosome Maxi Purification Kit for reliable, high-quality exosome isolation, enabling advanced research and application in various biological fields.Genaxxon also offers a Exosome Protein Purification Kit (S5328), and other Exosome purification kits for your specific needs (Exosome Mini Purification Kit (S5321) for small-volume samples or the Serum Exosome Purification Kit (S5323)).

Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments We use magnetic beads with a ferrimagnetic core and an agarose coating coupled to a ligand of choice, our beads meet our own high quality standards. They enable fast and easy purification steps, which can be automated. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. The rho1D4 purification system is based on the highly specific binding of the rho1D4 antibody to the rho1D4 epitope fused to proteins. This system has proven particularly effective with membrane proteins. Magnetic beads are useful for protein purification of dilute samples, and for pull-down experiments to determine protein-protein interactions. Elution of the protein can be done the rho1D4 peptide, which competitively binds to the rho1D4 antibody, thereby release the traget protein. The peptide can be purchased individually, or together with Genaxxon Rho1D4 MagBeads as part of a Starter Set. A 5% suspension is provided that binds up to 3mg of protein per mL settled beads. Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

Proteins tagged with the epitope sequence of the Rho1D4 antibody can be purified with this affinity matrix. Rho1D4 peptide in the Elution Buffer serves to competitively bind to the affinity resin and release the tagged protein, providing gentler elution conditions than, for example, changing pH. Genaxon bioscience offers conveniently aliquoted Rho1D4 peptide that has been tested with Rho1D4 Agarose. Based on BioWorks Workbeads, this product is the first commercially available immunoaffinity resin for the Rho1D4 purification system. Properties: - demonstrably suitable for membrane protein research - binding capacity of 3-4mg protein per mL resin - purifies protein with high specificity - gentle protein elution based on competitive binding - can be regenerated for reuse. Genaxxon resins are produced under strict quality guidelines and each batch undergoes quality checks to ensure that the loaded matrix has a high protein capacity. Combined with the specificity of the antibody-epitope interaction, a purification protocol optimized for the target protein can generate elution fractions with exceptionally high yields. This set offers both the Rho1D4 agarose plus the Rho1D4 peptide for elution. Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

Genaxxon bioscience offers pre-packed Ni2+ IDA columns in ready-to-use format for His-tag protein purification processes by gravity flow. Fast purifications with good yield of target proteins are obtainable by this method. Both matrices Ni2+- and Co2+-IDA-Agarose and 2 different bed volumes (1mL, 5mL) are available supplied as suspension in 20% ethanol. Frit Pore Size 20µm - 6% crosslinked agarose (Sepharose®CL-6B) -Matrix Stability in all commonly used reagents for protein purifications - Activating Agent Epichlorohydrin -Bed Volume Agarose 1mL (for L-Column), 5mL (for XL-Column) - Autoclavable at 121°, 30 min. - Capacity ca. 28 µmol Ni2+/mL gel, ca. 25 µmol Co2+/mL gel.

Set of Rho1D4 agarose plus the Rho1D4 peptide for elution. Proteins tagged with the epitope sequence of the Rho1D4 antibody can be purified with this affinity matrix. Rho1D4 peptide in the Elution Buffer serves to competitively bind to the affinity resin and release the tagged protein, providing gentler elution conditions than, for example, changing pH. Genaxon bioscience offers conveniently aliquoted Rho1D4 peptide that has been tested with Rho1D4 Agarose. Based on BioWorks Workbeads, this product is the first commercially available immunoaffinity resin for the Rho1D4 purification system. Properties:- demonstrably suitable for membrane protein research - binding capacity of 3-4mg protein per mL resin - purifies protein with high specificity - gentle protein elution based on competitive binding - can be regenerated for reuse. Genaxxon resins are produced under strict quality guidelines and each batch undergoes quality checks to ensure that the loaded matrix has a high protein capacity. Combined with the specificity of the antibody-epitope interaction, a purification protocol optimized for the target protein can generate elution fractions with exceptionally high yields. This set offers both the Rho1D4 agarose plus the Rho1D4 peptide for elution.

The GST tag is a 26 kDa protein that binds to glutathione-coupled agarose resins. This tag is considerably larger than the His tag and is often chosen to promote recombinant protein folding. In a comparative study with equivalent, market-leading resins, Geanxxon's Glutathione Agarose proved to be a competitive alternative, exhibiting the same protein binding capacity. Compared to competitor G both resins yielded up to 10mg/mL protein. Protein binding capacity: up to 10mg/mL. Compatible with a range of detergents and additives.

NTA-Agarose consists of the tetradentate chelating agent, nitrilotriacetic acid (NTA), covalently coupled agarose beads, and is loaded with Co2+ ions. In processing recombinant fusion proteins from eukaryotes or in the case of special protein complexes, Genaxxon Co-NTA Agarose should always be used offering the possibility of higher protein purity levels. Both metal ions feature differing spatial properties and electronic binding potentials. While Co2+ binds only domains adjacent to residual histidine and is thus highly selective for 6×histidine-tagged fusion proteins, Ni2+ ions are less specific and can bind residual histidine outside the polyhistidine tag, which may result in higher contamination with foreign proteins. The unique production process yields a Co-NTA Agarose that exhibits a higher protein binding capacity than that of leading competitor products. Genaxxon Co-NTA Agarose is very robust in the presence of DTT and EDTA. In a stability test, Genaxxon Co-NTA Agarose was exposed to increasing concentrations of DTT or EDTA for 1h. Thereafter, the resins were used to purify E. coli-expressed GFP-His in gravity columns. The binding capacity of the resin decreased in the presence of both DTT and EDTA but the decay rate was shallow. Genaxxon NTA Agarose is supplied as a 50% buffered suspension in 20% Ethanol.

Rho1D4 peptide in the Elution Buffer serves to competitively bind to the affinity resin and release the tagged protein, providing gentler elution conditions than, for example, changing pH. Genaxxon offers conveniently aliquoted Rho1D4 peptide that has been tested with our Rho1D4 Agarose. The peptide can be purchased individually, or together with Genaxxon Rho1D4 Agarose as part of a Starter Set. 5mg peptide is sufficient for 1mL Rho1D4 Agarose or MagBeads. Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

Empty Spin Columns for standard protein purification procedures, e.g. affinity chromatography with Ni-IDA, Ni-NTA, Co-IDA oder Co-NTA agaroses. The smallest column size does have a capacity of 0.5mL (S) and are ideal for protein optimisation process screenings. Our L columns (20mL) are a very cost effecitve option if you like to order IDA- or NTA-agarose in bulk and fill columnes by yourself. Our empty spin columns are offered in the sizes: 0.5mL (S), and 20mL (L). Easy Handling. Useable under native and denaturing conditions. For being able to use our spin columns you need a centrifuge with fixed angle (45° for our 600µL columns), or with a swing bucket rotor (for the 20mL columns).

Ni-NTA agarose was developed for affinity purification of proteins bearing a polyhistidine label. This affinity chromatography matrix is based on BioWorks workbeads consisting of 7.5% cross-linked agarose. The material is highly porous to allow optimal protein interaction. Cross-linked agarose is also physically very stable and suitable for low pressure purification processes with flow rates up to 6mL/min (optimal 0.5 - 2mL/min). Our Ni-NTA agarose has a very homogeneous size with a mean particle diameter of 40μm, resulting in a high degree of reproducibility between individual purification runs. Ni-NTA agarose consists of cross-linked agarose to which tetradendritic nitrilotriacetic acid (NTA) loaded with Ni2+ ions is covalently bound. The binding capacity varies with different proteins based on their specific properties, but it is at least 50mg target protein per mL agarose gel. The resin is ideally suited for batch, spin and column applications of any scale and offers a high degree of reproducibility between individual purification approaches with a very homogeneous size distribution around a mean particle diameter of 40µm. The Ni-NTA agarose is supplied as a buffered suspension in 50% ethanol. For example, 1x S5377.0010 corresponds to a suspension of 10mL resin and 10mL water with 20% EtOH. Total supplied volumes is 20mL.

Nickel-loaded agaroses are the most frequently used products for the efficient purification of 6×histidine-tagged recombinant fusion proteins. Because Ni2+ ions show high affinity for 6×histidine-tagged fusion proteins, extremely high yields result from purification of these fusion proteins from the lysates. However, if purification takes place from lysates containing foreign protein chains with repeated histidine elements, selectivity with respect to 6×histidine-tagged fusion protein decreases as non-tagged histidine-containing proteins increasingly also bind to the Ni-IDA agarose. In this case the use of Genaxxon Co-IDA Agarose is recommend (cat#: S5370), which features far higher selectivity with respect to 6×histidine-tagged fusion protein. Genaxxon Ni-IDA Agarose has high chemically and pH stability and enables purification directly from raw lysates. Genaxxon Ni-IDA Agarose can be regenerated several times with nickel sulfate solution for re-use. Features of Ni-IDA-Agarose - a highly efficient and favorable way for the separation of 6-His tagged fusion proteins. Highlights: - ideal for grafity-flow and batch procedures - no expensive purification system necessary - consistent performance - for native and denaturing conditions. The Genaxxon Ni-IDA Agarose consists of cross-linked Agarose, covalently bound to Iminodiacetic acid loaded with Ni2+-ions. Binding capacity: up to 50mg 6xHis tagged fusion protein per mL Agarose. Application: Affinity chromatography of 6-His tagged fusion proteins in batch or grafity flow procedure. Ni-IDA Agarose is supplied as a 50% buffered suspension with 20% Ethanol.

Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments We use magnetic beads with a ferrimagnetic core and an agarose coating coupled to a ligand of choice, our beads meet our own high quality standards. They enable fast and easy purification steps, which can be automated. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Genaxxon MagBeads are ferrimagnetic agarose beads coupled to a chelating ligand (IDA or NTA) coordinating nickel or cobalt ions. All offered ligand-metal systems efficiently bind histidine-tagged proteins. With a binding capacity of up to 40mg protein per mL (Ni-IDA) and 70mg per mL (Ni-NTA) of settled MagBeads, Genaxxon Ni-IDA MagBeads are comparable to equivalent beads from alternative providers.

Genaxxon's Exosome Protein Extraction Kit is especially engineered to extract proteins from various exosomes with high efficiency. The kit is the perfect solution for researchers aiming for high-quality Western Blot results.Key Features: High Protein Yield: Achieve a high concentration of exosome proteins, ensuring optimal results for your Western Blot experiments. Effective Lysis: The kit contains a special protein denaturant that effectively lyses exosomes, maximizing protein extraction. Easy to Use: Simply mix the kit with your exosome solution to lyse the exosomes. The lysate can then be directly loaded into SDS-PAGE for straightforward analysis. Accurate Quantification: Measure the concentration of exosome proteins in the lysates using either the Bradford or BCA protein concentration assay methods. Western Blot Ready: The denatured proteins are perfectly suited for Western Blot applications, ensuring reliable and high-quality results. Note: These proteins are not suitable for ELISA or enzyme activity measurements. Applications: Western Blot Analysis: Ideal for researchers focused on obtaining high-quality Western Blot data. Protein Quantification: Compatible with Bradford and BCA assay methods for accurate protein concentration measurement. Choose the Genaxxon Exosome Protein Extraction Kit for a reliable, efficient, and high-yield extraction of exosome proteins, tailored specifically for Western Blot success. 

Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments We use magnetic beads with a ferrimagnetic core and an agarose coating coupled to a ligand of choice, our beads meet our own high quality standards. They enable fast and easy purification steps, which can be automated. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Genaxxon MagBeads are ferrimagnetic agarose beads coupled to a chelating ligand (IDA or NTA) coordinating nickel or cobalt ions. All offered ligand-metal systems efficiently bind histidine-tagged proteins. With a binding capacity of up to 40mg protein per mL (Ni-IDA) and 70mg per mL (Ni-NTA) of settled MagBeads, Genaxxon Ni-IDA MagBeads are comparable to equivalent beads from alternative providers.

Empty Spin Columns for standard protein purification procedures, e.g. affinity chromatography with Ni-IDA, Ni-NTA, Co-IDA oder Co-NTA agaroses. The smallest column size does have a capacity of 0.5mL (S) and are ideal for protein optimisation process screenings. Our L columns (20mL) are a very cost effecitve option if you like to order IDA- or NTA-agarose in bulk and fill columnes by yourself. Our empty spin columns are offered in the sizes: 0.5mL (S) >, and 20mL (L) >. Easy Handling. Useable under native and denaturing conditions. For being able to use our spin columns you need a centrifuge with fixed angle (45° for our 500µL columns), or with a swing bucket rotor (for the 20mL columns).

Glutathione MagBeads are developed for the affinity purification of glutathione-S-transferase (GST) fusion proteins. The affinity matrix is based on spherical magnetic cross-linked agarose. The material is highly porous to allow optimal protein interaction. Cross-linked agarose is also physically very stable, making it suitable for purification processes without deformation or destruction. Our magnetic beads are very homogeneous in size with a medium particle diameter of 30µm, yielding a high degree of reproducibility between individual purification runs.Glutathione is coupled to the magnetic agarose beads to obtain an affinity matrix with highest binding capacity for GST fusion proteins. Because the purification method depends on correctly folded GST protein, only native conditions can be used.Glutathione MagBeads are delivered as a 25% suspension in 20% ethanol. Therefore, 1mL suspension will yield a 250µL bed volume. The suspension contains 20% ethanol to prevent microbial growth. Genaxxon MagBeads have a binding capacity of >10mg protein/mL settled beads. Protein Binding CapacityThe protein binding capacity is up to 10mg/mL settled beads, as determined by purification of glutathione-S-transferase (GST) from E.coli cleared lysates, and quantified via spectrophotometry. GST-tagged proteins expressed in bacteria, yeasts, insects and in cell culture supernatants can be readily purified in a single purification step even from proteins expressed at low levels. After binding of target molecules, the magnetic resin can be readily isolated with the aid of a magnet. The GST tag can be cleaved in bound condition or in eluted condition by specific proteases such as TEV-express. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Genaxxon MagBeads are ferrimagnetic agarose beads coupled to glutathione, an efficient ligand for GST fusion proteins. Human rhodopsin is a G-protein coupled receptor and an integral membrane protein of the light-absorbing molecules that mediate vision. It consists of a protein, opsin, which is covalently bound to 11-cis-retinal. Rhodopsin expression has been reported in rod-shaped photoreceptor cells of the retina. Retinitis pigmentosa is a hereditary disease of photoreceptor degeneration. Phosphorylation of rhodopsin at multiple phosphorylation sites is required for its rapid and reproducible deactivation.

Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments We use magnetic beads with a ferrimagnetic core and an agarose coating coupled to a ligand of choice, our beads meet our own high quality standards. They enable fast and easy purification steps, which can be automated. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Genaxxon MagBeads are ferrimagnetic agarose beads coupled to a chelating ligand (IDA or NTA) coordinating nickel or cobalt ions. All offered ligand-metal systems efficiently bind histidine-tagged proteins. With a binding capacity of up to 40mg protein per mL (Ni-IDA) and 70mg per mL (Ni-NTA) of settled MagBeads, Genaxxon Ni-IDA MagBeads are comparable to equivalent beads from alternative providers.

CentriPure P100 columns are pre-hydrated gel filtration columns for protein purification and desalting. CentriPure P100 Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins. The gel matrix of CentriPure is Zetadex-25, a beaded composite material developed by emp Biotech composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Zetadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for Zetadex-25 is 5 kD for proteins. Proteins larger than 5 kD in a sample volume of up to 10mL can be purified with an elution volume of 12mL to 15mL.

CentriPure Z25 Mini Spin columns are prehydrated (in water) Mini Columns used for quick and efficient desalting, buffer exchange and/or removal of dyes and small molecules (haptens, biotin, ammonium, etc.) from proteins greater than 5 kD. Purified proteins are eluted into pure, deionized water (Caution! Some proteins may precipitate in pure water with low ionic strength!). The columns are sterile packed, pre-swollen and ready-to-use. Removes up to 99.999% salts, dyes, haptens and other small molecules. Samples up to 100µL are processed in under 5 minutes!

CentriPure P50 columns are pre-hydrated gel filtration columns for protein purification and desalting. Processes sample volumes of 5mL. CentriPure P50 Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins. The gel matrix of CentriPure is Zetadex-25, a beaded composite material developed by emp Biotech composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Zetadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for Zetadex-25 is 5 kD for proteins. Proteins larger than 5 kD in a sample volume of 5 mL can be purified with an elution volume of 6 to 8 mL.

Hydrated gel filtration columns for protein purification and desalting. Processes sample volumes of 150 to 300µL. CentriPure 2 Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins. The gel matrix of CentriPure is Zetadex-25, a beaded composite material developed by emp Biotech composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Zetadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for Zetadex-25 is 5 kD for proteins. Proteins larger than 5 kD in a sample volume of up to 200µL can be purified with an elution volume of 200 to 350µL.

CentriPure P5 columns are pre-hydrated gel filtration columns for protein purification and desalting. Process sample volume of 500µL. CentriPure P5 Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins. The gel matrix of CentriPure is Zetadex-25, a beaded composite material developed by emp Biotech composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Zetadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for Zetadex-25 is 5 kDa for proteins. Proteins larger than 5 kDa in a sample volume of up to 650µL can be purified with an elution volume of 650 to 900µL.