Biochemicals and Biochemistry Products

Nicotinamide adenine dinucleotide phosphate reduced - Tetrasodium salt in short NADPH is the reduced version of NADP+ and can transfer a hydride atom to another reaction partner in a redox reaction. The abbreviations NADP+ for the oxidized form, NADPH for the reduced form and NADP in general are proposed by IUPAC. β-Nicotinamide adenine dinucleotide 2'-phosphate (NADP +) and β-nicotinamide adenine dinucleotide 2'-phosphate, reduced (NADPH), comprise a coenzyme redox couple (NADP+ : NADPH) involved in a variety of enzyme-catalyzed oxidation-reduction reactions. The NADP+ / NADPH redox couple facilitates electron transfer in anabolic reactions such as lipid and cholesterol biosynthesis and fatty acyl chain extension. The NADP+ / NADPH redox system is used in a variety of antioxidant mechanisms to prevent the accumulation of reactive oxidation products. NADPH is generated in vivo by the pentose phosphate pathway (PPP).

TLCK is an irreversible, specific inhibotor of the Serin-protease Trypsin and inhibits the Thrombin-like protease Cerastocytin. Together with the competitive inhibitors benzamidin hydrochlorid or 8M urea Trypsin does not show inhibitiory effects. Chymotrypsin is not inhibited by TLCK. The pH optimum for inhibition through Histidin modification is pH 7.5. Chymotrypsinogen is not inhibited by TLCK. A concentration of 50 µg/mL of TLCK inhibits 100% of the Trypsin like proteinase activity of wheat seat extracts. Besides proteases also protein kinases are inhibited by TLCK. Obviously by the modification of the enzymatic activity centre of the protein kinases. TLCK solutions are most stable at pH 6.0 at +4°C. TLCK is not stable at pH above 7.0. Therefore stock solutions of 1 mg/mL to 20 mg/mL are prepared with buffer solutions of pH below 6.0. Always pipete shortly before the inhibition experiment. Working solutionsa are: 50 µg/mL to 100 µg/mL. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.5M Tris buffered saline, 1.38M NaCl, 0.027M KCl, pH8.0 at 25°C.

Collagenase-Chromophore-Substrate Component A also named 4-Phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg-OH x 2H2O.

Synonyms: D-threo-pent-2-ulose. Xylulose a ketopentose, is a monosaccharide containing five carbon atoms, and including a ketone functional group. In nature, it occurs in both the L- and D-enantiomers. L-Xylulose accumulates in the urine in patients with pentosuria, due to a deficiency in L-xylulose reductase. Since L-xylulose is a reducing sugar like D-glucose, pentosuria patients have been wrongly diagnosed in the past to be diabetic.

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.89M Tris-borate, 0.02M EDTA, pH8.3 at 25°C. In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis.TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels. TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide. Medicago’s TBE and TAE buffers are supplied as a pre-weighed powder mix in sealed pouches giving 1000mL of 1X, 5X or 10X Tris-borate-EDTA buffer or 50X Tris-acetate-EDTA buffer with pH 8.3 at 25°C. You will find more ready-to-use buffers here: ready-to-use buffers >

Ready-to-use 100mM IPTG solution (Isopropyl-ß-D-thiogalactopyranoside) for molecular biology research. IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used together with X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactoside) or Bluo-Gal (5-Bromo-3-indolyl-beta-D-galactopyranoside) in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. While IPTG binds to the lacI repressor it is altering the conformation of the lacl repressor, which prevents the repression of the β-galactosidase coding gene lacZ. Thus IPTG is an inducer of the lac operon in bacteria for detection of lac+ colonies. Shipped as 10 x 1mL solution.

a-D-Mannose-1-phosphate dipotassium salt dihydrate (synthetic crystalline product). a-D-Mannopyranose-phosphate. Free carbonate and phosphate are completely removed. Product shipped with certificate covering all data for validation (NMR, FTIR, GC, GC-MS, AAS, HPLC, optical rotation and MP. Man-1-P.

Contents of 1 pouch dissolved in deionized water and made up to 500mL/1000mL yields: 2.0M Tris acetate buffer, 0.05M EDTA, pH8.3 at 25°C. In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis.TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels. TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide. Medicago’s TBE and TAE buffers are supplied as a pre-weighed powder mix in sealed pouches giving 1000mL of 1X, 5X or 10X Tris-borate-EDTA buffer or 50X Tris-acetate-EDTA buffer with pH 8.3 at 25°C.

Aphidicolin inhibits the growth of eukaryotic cells and certain animal viruses by selectively inhibiting the cellular replication of DNA polymerase II or the viral-induced DNA polymerases. The drug may be useful for controlling excessive cell proliferation in patients with cancer, psoriasis or other dermatitis with little or no adverse effect upon non-multiplying cells. Aphidicolin is a tool in cell proliferation and differentiation research. Aphidicolin may possibly be used for controlling excessive cell proliferation in cancer, psoriasis or other dermatitis with little or no adverse effect upon non-multiplying cells. Synonyms:(3-a,4-a,5-,17-a)-3,17-Dihydroxy-4-methyl-9,15-cyclo-C,18-dinor14,15-secoandrostane-4,17-dimethanole. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

ready-to-use PBS buffer tablets. Each tablet dissolved in 1000mL of deionized water yields: 0.01M Phosphate buffer, 0.0027M KCl, 0.14M NaCl, pH7.4 at 25°C. No time consuming weighing or measuring of pH necessary anymore. You can get rid of the whole validation process and you will have no need for scales or pH-meters anymore. Amoung biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns. Our PBS is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches. There are different standard-sized packages and final volumes range from 100mL to 100 litres. Choose from different pH (7.2 or 7.4), from PBS with our without potassium, or with and without Tween®. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >). PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

Ready-to-use PBS tablets, individually packed in a PE bag. Just dissolve 1 of  the PBS tablets in 1000mL  deionized water  to yield a 0.01M Phosphate buffer, 0.0027M KCl, 0.14M NaCl, pH7.4 at 25°C. No time consuming weighing or measuring of pH necessary anymore. You can get rid of the whole validation process and you will have no need for scales or pH-meters anymore. Amoung biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns. Our PBS is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches. There are different standard-sized packages and final volumes range from 100mL to 100 litres. Choose from different pH (7.2 or 7.4), from PBS with our without potassium, or with and without Tween®. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >).PBS with Tween™20, pH7.4 (D2003 (100mL and 500mL) > and (D2004 (1L) >).PBS, pH7.2 (D2034 >) PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

Precisely weighed ready-to-use PBS tablets with Tween®20 offered in plastic bottles or in practical blister packs. - no awkward weighing anymore- no toilsome pipetting/weighing of Tween®20 anymore- no more time-consuming pH adjustment- simply dissolve the tablet completely in demineralized water and use the buffer Ready-to-use PBS buffer tablets with Tween®20 containing the non-ionic detergent Tween®20 which acts through blocking. It has the ability to reduce non-specific binding and protein-protein interaction during the wash step in protein and immunoassay procedures such as ELISA and Western blotting. By decreasing the non-specific binding and staining makes interpretation of ELISA results and Western blots easier. Each tablet dissolved in deionized water yields a 0.14M sodium chloride 0.0027M potassium chloride, 0.05% Tween®20, 0.01M phosphate buffer with pH7.4 at 25°C. Final buffer volumes are: 100mL and 500mL (D2003 >) or 1L (D2004 >). Our ready-to-use tablets are also available as PBS tablets without Tween®20, as TBS or as IMAC tablets for affinity chromatography. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >). PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

Medicago’s pNPP substrate is specifically developed for immunoassay procedures and it is ideal for phosphate-based ELISA methods. The substrate is also used as a chromogenic non-specific substrate in alkaline and acid phosphatase assays. Its soluble end-product is yellow and can be spectrophotometrically read at 405 to 410 nm. The reaction may be stopped with 3 M NaOH. The pNPP substrate is supplied as pre-weighed tablets in bottles or in convenient blister packs, each tablet containing 5 mg or 20 mg of substrate. Directions for use:Deposit one tablet of the pNPP substrate in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized water in the appropriate amount to reach the required concentration for the assay. Stir until full dissolution and the substrate solution is ready to use.

Etoposide phosphate (Eposin®, Etopophos®, Vepesid®, VP-16®) is an inhibitor of the enzyme topoisomerase II. For medical purposes it is used as a form of chemotherapy for malignancies such as lung cancer, testicular cancer, lymphoma, non-lymphocytic leukemia, and glioblastoma multiforme. It is often given in combination with other drugs. Chemically it derives from podophyllotoxin, a toxin found in the American Mayapple. Genaxxon offers this product only for in-vitro (laboratory) usage! You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

X-alpha-D-Gal is a chromogenic substrate for alpha-galactosidase yielding a blue precipitate (X-alpha-Gal is a chromogenic substrate for yeast galactosidase (MEL1) and is used for detecting GAL4-based yeast two-hybrid interactions directly on agar). Used for species differentiation within the family Enterobacteriaceae and differentiation of Bifidobacteria species from Lactobacillus species (see references). Synonmys: 5-Bromo-4-chloro-3-indolyl a-D-galactopyranoside, X-α-Gal; X-alpha-Gal. Other products that are used as a substrate for alpha or beta-Galactosidase: M3225: 5-Bromo-3-indolyl-beta-D-galactopyranoside >M3204: 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal) >M3205: 5-Bromo-4-chloro-3-indolyl-ß-D-glucopyranoside (X-Glu / X-Glc) > References: 1. Aho S., Arffman A., Pummi T., Uitto J., A novel reporter gene MEL1 for the yeast two-hybrid system. Anal Biochem. 1997, 253, 270-2. 2. Chevalier P., et al., X-alpha-Gal-base medium for simultaneous enumeration of bifidobacteria and lactic acid bacteria in milk. J. Microbiol. Methods 1991, 13, 75. 3. Gossrau R., Lojda Z., Histochemical detection of alpha-D-galactosidase with 5-Br-4-Cl-3-indoxyl alpha-D-galactoside. Acta Histochem. 1989, 85, 213-22. 4. Perry J.D., Ford M., Taylor J., Jones A.L., Freeman R., Gould F.K., ABC medium, a new chromogenic agar for selective isolation of Salmonella spp. J. Clin. Microbiol. 1999, 37, 766-8.

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 1M Tris-HCl buffer, pH7.4 at 25°C. Sterilization can be performed by filtration. Filtrate the buffer solution through a 0.22µm filter into a sterile flask. Keep the buffer solution at +2°C to +8°C. The pH value of a Tris buffer strongly depends on the temperature. The pKa of 8.06 changes approximately 0.03 units per degree Celsius.

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 1M Tris-HCl buffer, pH8.3 at 25°C. Sterilization can be performed by filtration. Filtrate the buffer solution through a 0.22µm filter into a sterile flask. Keep the buffer solution at +2°C to +8°C. The pH value of a Tris buffer strongly depends on the temperature. The pKa of 8.06 changes approximately 0.03 units per degree Celsius.

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 1M Tris-HCl buffer, pH8.0 at 25°C. Sterilization can be performed by filtration. Filtrate the buffer solution through a 0.22µm filter into a sterile flask. Keep the buffer solution at +2°C to +8°C. The pH value of a Tris buffer strongly depends on the temperature. The pKa of 8.06 changes approximately 0.03 units per degree Celsius.

2-[Bis(2-hydroxyethyl)imino]-2-(hydroxymethyl)-1,3-propandiol. Puffersubstanz: Daboo M. and Bates R. (1970) J. Phys. Chem., 74, 702-5. Bis-Tris is an amino buffer very similar in its chemical structure to Trizma ("Tris"). Bis-Tris is a frequently used important buffer if working with proteins or nucleic acids. Bis-Tris is also used very often zu replace buffers that contain Cacodylic acid (Dimethylarsinic acid).

pNPh-a-D-Man, CAS: [10357-27-4] - C12H15NO8

TPCK is an irreversible, specific inhibotor for chymotrypsin and inhibits the thrombin-like protease Cerastocytin. Trypsin is not inhibited by TPCK. The pH optimum for inhibition throµgh alkylation by TPCK of Chymotrypsin is pH7.2. Chymotrypsinogen is not inhibited by TPCK. The bacterial Serin-proteases Re, Fa and So from E.coli are completely inhibited by 0.5 to 1 mM TPCK. TPCK is used for the production of Chymotrypsin free Trypsin. TPCK solutions are stable at pH below 7.0 at RT, nevertheless it is recommended to store solutions at +2°C to +8°C. TPCK is not stable at pH above 7.0. Stock solutions of 3mg/mL to 20mg/mL are prepared in MeOH or EtOH. Working concentrations: 10µg/mL to 100µg/mL. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Adenosine triphosphate (ATP) is the universal and immediately available energy source in cells and an important regulator of energy providing processes. The molecule adenosine triphosphate consists of an adenine, the sugar ribose and three phosphate (α to γ) in ester (α) or anhydride bond (β and γ). It was shown that ATP has an inhibitory effect on the inflammatory response by abrogating secretion of TNF-α and promoting secretion of Interleukin-10.  Synonyms: ATP, Adenosin-5'-Triphosphat.  Also available from Genaxxon: NADH Na salt (reduced) >.

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.445M Tris-borate, 0.01M EDTA, pH8.3 at 25°C. In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis. TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels. TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide. Medicago’s TBE and TAE buffers are supplied as a pre-weighed powder mix in sealed pouches giving 1000mL of 1X, 5X or 10X Tris-borate-EDTA buffer or 50X Tris-acetate-EDTA buffer with pH 8.3 at 25°C. You will find more ready-to-use buffers here: ready-to-use buffers >

Precisely weighed ready-to-use PBS buffer tablets offered in plastic bottles or in practical blister packs. - no awkward weighing anymore- no more time-consuming pH adjustment- simply dissolve the tablet completely in demineralized water and use the buffer 1 tablet dissolved in 500mL of deionized water yields: 0.01M Phosphate buffer, 0.0027M KCl, 0.14M NaCl, pH7.4 at 25°C. Amoung biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns. Our PBS is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches. There are different standard-sized packages and final volumes range from 100mL to 100 litres. Choose from different pH (7.2 or 7.4), from PBS with our without potassium, or with or without Tween. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >). PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

Acetyl Coenzyme A (Ac CoA) is the end product of glycolysis and takes part in the Ac CoA pathway, which is a metabolic pathway for carbon compounds. Ac-CoA is important in cholesterol synthesis. Fatty acids are relatively unreactive and must therefore be activated before reactions with coenzyme A. The key enzyme is the Acyl CoA synthetase, also called thiokinase. The fatty acid is first formed by the thiokinase (attachment of AMP) to acyl adenylate. Acyl adenylate reacts under the cleavage of AMP and the addition of CoA (catalyzed by the thiokinase) to form Acyl CoA. Accordingly, acetic acid can also be activated and forms Acetyl CoA. Concentration and stabilityWorking solution: Optimal solvent: water or aqueous solution with a weak acidic pH (4 to 5).Storage conditions of the working solution: -15°C to -25°C.A solution of 50mg/mL in phosphate buffer, pH7, is stable for 3 weeks at -15°C to -25°C. Unfrozen solutions should be used immediately.

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used together with X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactoside) or Bluo-Gal (5-Bromo-3-indolyl-beta-D-galactopyranoside) in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. Mode of operation:While IPTG binds to the lacI repressor it is altering the conformation of the lacl repressor, which prevents the repression of the β-galactosidase coding gene lacZ. Thus IPTG is an inducer of the lac operon in bacteria for detection of lac+ colonies. Dissolve in water to e.g. 200mg/mL, sterilise by filtration and store in aliquots at -20°C. For detection of transformants, use in final concentration of 0.1mM up to 1mM in LB-Medium.

Polyethylene glycol 400, PEG, Polyoxyethylen, Polyglycol.

Precisely weighed ready-to-use PBS buffer tablets offered in plastic bottles or in practical blister packs. - no awkward weighing anymore- no more time-consuming pH adjustment- simply dissolve the tablet completely in demineralized water and use the buffer 1 tablet dissolved in 500mL of deionized water yields: 0.01M Phosphate buffer, 0.0027M KCl, 0.14M NaCl, pH7.4 at 25°C. Amoung biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns. Our PBS is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches. There are different standard-sized packages and final volumes range from 100mL to 100 litres. Choose from different pH (7.2 or 7.4), from PBS with our without potassium, or with or without Tween. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >). PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

pNPh-a-L-Fuc, CAS: [22153-71-5] - C12H15NO7

Content of 1 pouch dissolved in deionized water and made up to 500mL/1000mL yields: 0.5M EDTA, pH8.0 at 25°C. EDTA (ethylene-diamine-tetraacetic acid) is a chelating agent widely used in molecular biology to sequester divalent and trivalent metal ions such as calcium and magnesium. This ability prevents DNA and RNA degradation as metal-dependent enzymes acting as nucleases become deactivated. A fully deprotonated EDTA molecule will bind directly to the metal ion making the buffer suitable for adding to stored blood as an anti-coagulant to bind Ca2+ ions. Furthermore, EDTA is useful for cell culture procedures as it prevents clumping of cells in liquid suspension and detaches adherent cells when passaging. Genaxxon’s EDTA buffer is supplied in two sizes with pouches giving 500mL or 1000mL of 0.50M EDTA buffer with pH8.0 at 25°C when the contents of one pouch is dissolved in deionized water.

AEBSF is an irreversible inhibitor of Thrombin and other Serin proteases (Chymotrypsin, Kallikrein, Plasmin, Proteinase K, Trypsin) by sulfonylation of a functional group in the active centre of the enzyme. AEBSF is much less toxic compared to PMSF and DFP and therefore recommended to substitute these reagents. AEBSF is highly soluble in water resulting in a solution of pH 6.5. At this pH AEBSF is most stable. We recommend to store stock solutions (20 mM or 100 mM in water or buffer) at -20°C for up to 2 months and prepare fresh working solutions. AEBSF is used at a working concentration of 0.1 - 0.2 mM. Walsmann, P. et al. (1972) Acta biol. med. germ. 28, 577-585, Nathan, D.F. & Linquist, S. (1995) Mol. Cell. Biol., 15, 3917-3925. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 1M Sodium phosphate buffer, pH6.5 at 25°C. Other phosphate-based ready-to-use buffers:: PBS without Potassium (0.01M) (D2033 >). PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 1M Sodium phosphate buffer, pH7.2 at 25°C. Other phosphate-based ready-to-use buffers:: PBS without Potassium (0.01M) (D2033 >).PBS with Tween™20, pH7.4 (D2003 (100mL and 500mL) > and (D2004 (1L) >).PBS, pH7.2 (D2034 >) PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

a-D-Galactose-1-phosphate dipotassium salt dihydrate (synthetic crystalline product). Free carbonate and phosphate are completely removed. a-D-Galactose-1-phosphate. Product shipped with certificate covering all data for validation (NMR, FTIR, GC, GC-MS, AAS, HPLC, optical rotation and MP. Gal-1-P.

pNPh-b-D-GlcNAc - CAS: [3459-18-5] - C14H18N2O8