Biochemicals and Biochemistry Products
High quality biochemicals for a wide range of applications in natural sciences can be found at Genaxxon bioscience. Our products meet high quality standards and are specifically designed for research purposes. For reproducible results you will find products from following categories:
Carbohydrates - Detergents - Non-Ionic Detergents - Substrates - Buffers - Ready-to-Use Buffers
1-Thio-ß-D-glucose sodium salt dihydrate
Purity: >99% (HPLC). CAS: [10593-29-0] - C6H11NaO5S x 2 H2O
Variants from €132.19
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€401.31
10X Phosphate buffered saline powder (pH7.4) - 5 bags
Among biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns.
This PBS buffer is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches.
How to prepare buffer solution:
Empty one pouch of NaPi buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 200mL to 300mL of deionized water for a 1000mL pouch and stir the solution for a few minutes. Adjust the volume up to 1000mL, stir and the buffer solution is ready to use.Other phosphate-based ready-to-use buffers::PBS without Potassium (0.01M) (D2033 >).
PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >).
Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >
10X Tris buffered saline (pH8.0) - 10 bags
Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.5M Tris buffered saline, 1.38M NaCl, 0.027M KCl, pH8.0 at 25°C.
10X Tris-EDTA buffer (pH7.4) - 10 bags
10 bags of a 10-fold Tris-EDTA buffer (pH7.4) - ready-to-use Tris/HCl-EDTA powder mixture for 1L ready-to-use buffer solution of pH7.4.
This TE buffer is composed by Tris, a buffering agent and EDTA. EDTA has the ability to prevent degradation of DNA and RNA by chelating magnesium- or other divalent metal ions.Tris-EDTA-based solutions break protein cross-links and can therefore unmask antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections.
Treatment with TE buffer enhances the staining intensity of antibodies in the immuno-histochemical detection of certain proteins.
TE buffer powder is supplied as exactly pre-weighed powder in pouches. To prepare the buffer, the content of a bag is simply dissolved in 1L of deionized water. This results in a 0.1M Tris/HCl, 0.01M EDTA buffer of pH7.4 at 25°C.
T
3,3'5,5'-Tetramethylbenzidine (TMB)
Substrate for horseradish peroxidase detection assays.
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€584.94
4-Chloro-1-Naphthol (min. 99.0% HPLC)
4-Chloro-1-naphtol is peroxidase substrate suitable for use in immunoblotting. The endproduct of the enzymatic reaction is an insoluble blue precipitate that can be observed visually. The precipitate can is not removed by washing with water.
Variants from €112.09
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€316.46
4-Nitrophenyl a-D-manopyranoside - min. 99%
pNPh-a-D-Man, CAS: [10357-27-4] - C12H15NO8
Variants from €337.77
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€1,101.23
4-Nitrophenyl a-L-fucopyranoside - min. 99%
pNPh-a-L-Fuc, CAS: [22153-71-5] - C12H15NO7
Variants from €241.24
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€786.61
4-Nitrophenyl b-D-xylopyranoside - min. 99%
pNPh-b-D-Xyl - CAS: [2001-96-9] - C11H13NO7
Variants from €202.66
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€723.69
4-Nitrophenyl-2-acetamido-2-deoxy-b-D-glucopyranoside (min. 99.0% HPLC)
pNPh-b-D-GlcNAc - CAS: [3459-18-5] - C14H18N2O8
Variants from €211.86
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€859.56
5-Bromo-4-chloro-3-indolyl a-D-galactopyranoside / X-alpha-Gal
X-alpha-D-Gal is a chromogenic substrate for alpha-galactosidase yielding a blue precipitate (X-alpha-Gal is a chromogenic substrate for yeast galactosidase (MEL1) and is used for detecting GAL4-based yeast two-hybrid interactions directly on agar). Used for species differentiation within the family Enterobacteriaceae and differentiation of Bifidobacteria species from Lactobacillus species (see references). Synonmys: 5-Bromo-4-chloro-3-indolyl a-D-galactopyranoside, X-α-Gal; X-alpha-Gal.
Other products that are used as a substrate for alpha or beta-Galactosidase:
M3225: 5-Bromo-3-indolyl-beta-D-galactopyranoside >M3204: 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal) >M3205: 5-Bromo-4-chloro-3-indolyl-ß-D-glucopyranoside (X-Glu / X-Glc) >
References: 1. Aho S., Arffman A., Pummi T., Uitto J., A novel reporter gene MEL1 for the yeast two-hybrid system. Anal Biochem. 1997, 253, 270-2. 2. Chevalier P., et al., X-alpha-Gal-base medium for simultaneous enumeration of bifidobacteria and lactic acid bacteria in milk. J. Microbiol. Methods 1991, 13, 75. 3. Gossrau R., Lojda Z., Histochemical detection of alpha-D-galactosidase with 5-Br-4-Cl-3-indoxyl alpha-D-galactoside. Acta Histochem. 1989, 85, 213-22. 4. Perry J.D., Ford M., Taylor J., Jones A.L., Freeman R., Gould F.K., ABC medium, a new chromogenic agar for selective isolation of Salmonella spp. J. Clin. Microbiol. 1999, 37, 766-8.
Variants from €364.65
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€642.48
5% SDS Solution
5% SDS solution as cleansing solution for lenses of optical systems like lathe from EOS GmbH (Electro-Optical Systems). Filtered and ready-to-use. SDS may precipitate at temperature below 15°C. Dissolve precipitated SDS by warming up in a water bath at 37°C.
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€38.77
50X Tris-Acetate-EDTA buffer (pH8.3 - 5 bags)
Contents of 1 pouch dissolved in deionized water and made up to 500mL/1000mL yields: 2.0M Tris acetate buffer, 0.05M EDTA, pH8.3 at 25°C. In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis.TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels. TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide. Medicago’s TBE and TAE buffers are supplied as a pre-weighed powder mix in sealed pouches giving 1000mL of 1X, 5X or 10X Tris-borate-EDTA buffer or 50X Tris-acetate-EDTA buffer with pH 8.3 at 25°C.
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5X TBE buffer (pH8.3) - 10 bags
Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.445M Tris-borate, 0.01M EDTA, pH8.3 at 25°C. In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis. TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels. TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide. Medicago’s TBE and TAE buffers are supplied as a pre-weighed powder mix in sealed pouches giving 1000mL of 1X, 5X or 10X Tris-borate-EDTA buffer or 50X Tris-acetate-EDTA buffer with pH 8.3 at 25°C.
You will find more ready-to-use buffers here: ready-to-use buffers >
5X TBE buffer (pH8.3) - 10 bags
Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.89M Tris-borate, 0.02M EDTA, pH8.3 at 25°C (pH value of a 1X buffer solution).The buffer does not require the use of calibration or measuring equipment. No time-consuming or costly validation procedures are needed. Simply dissolve the powder and use it!Instructions for use:Transfer the contents of one sachet of TAE or TBE buffer powder into a beaker placed on a magnetic stirrer. Add 300mL of deionised water and stir the solution for a few minutes. Then make up the volume to 1000mL with deionised water. Continue stirring until everything has completely dissolved. The buffer solution is now ready for use!
You will find more ready-to-use buffers here: ready-to-use buffers >
a-D-Galactopyranose-phosphate di-potassium salt dihydrate (min. 99.0% HPLC)
a-D-Galactose-1-phosphate dipotassium salt dihydrate (synthetic crystalline product). Free carbonate and phosphate are completely removed. a-D-Galactose-1-phosphate. Product shipped with certificate covering all data for validation (NMR, FTIR, GC, GC-MS, AAS, HPLC, optical rotation and MP. Gal-1-P.
Variants from €234.54
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€925.73
a-D-Glucopyranose-phosphate di-potassium salt dihydrate (min. 99.0% HPLC)
a-D-Glucose-1-phosphate dipotassium salt dihydrate, Cori-Ester di-K (synthetic crystalline product). a-D-Glucopyranose-phosphate. Free carbonate and phosphate are completely removed. Product shipped with certificate covering all data for validation (NMR, FTIR, GC, GC-MS, AAS, HPLC, optical rotation and MP. Glc-1-P.
Variants from €71.60
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€293.66
a-D-Mannopyranose-phosphate di-potassium salt dihydrate (min. 99.0% HPLC)
a-D-Mannose-1-phosphate dipotassium salt dihydrate (synthetic crystalline product). a-D-Mannopyranose-phosphate. Free carbonate and phosphate are completely removed. Product shipped with certificate covering all data for validation (NMR, FTIR, GC, GC-MS, AAS, HPLC, optical rotation and MP. Man-1-P.
Variants from €398.22
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€1,522.28
Acetyl Coenzyme A (>83% enzymatic)
Acetyl Coenzyme A (Ac CoA) is the end product of glycolysis and takes part in the Ac CoA pathway, which is a metabolic pathway for carbon compounds. Ac-CoA is important in cholesterol synthesis. Fatty acids are relatively unreactive and must therefore be activated before reactions with coenzyme A. The key enzyme is the Acyl CoA synthetase, also called thiokinase. The fatty acid is first formed by the thiokinase (attachment of AMP) to acyl adenylate. Acyl adenylate reacts under the cleavage of AMP and the addition of CoA (catalyzed by the thiokinase) to form Acyl CoA. Accordingly, acetic acid can also be activated and forms Acetyl CoA.
Concentration and stabilityWorking solution: Optimal solvent: water or aqueous solution with a weak acidic pH (4 to 5).Storage conditions of the working solution: -15°C to -25°C.A solution of 50mg/mL in phosphate buffer, pH7, is stable for 3 weeks at -15°C to -25°C. Unfrozen solutions should be used immediately.
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AEBSF Hydrochloride
AEBSF is an irreversible inhibitor of Thrombin and other Serin proteases (Chymotrypsin, Kallikrein, Plasmin, Proteinase K, Trypsin) by sulfonylation of a functional group in the active centre of the enzyme. AEBSF is much less toxic compared to PMSF and DFP and therefore recommended to substitute these reagents. AEBSF is highly soluble in water resulting in a solution of pH 6.5. At this pH AEBSF is most stable. We recommend to store stock solutions (20 mM or 100 mM in water or buffer) at -20°C for up to 2 months and prepare fresh working solutions. AEBSF is used at a working concentration of 0.1 - 0.2 mM. Walsmann, P. et al. (1972) Acta biol. med. germ. 28, 577-585, Nathan, D.F. & Linquist, S. (1995) Mol. Cell. Biol., 15, 3917-3925.
You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.
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Aprotinin from bovine lung
Description:Aprotinin is mainly obtained from bovine lung. Aprotinin consists of 58 amino acids of 6511 Da and is very basic, whereby amino acids 13-18 of Aprotinin are of particular importance for binding to serine proteases. As Aprotinin has been described by various research groups, two names have become established: 1. bovine pancreatic trypsin inhibitor (BPTI) and 2. trypsin-kallikrein inhibitor (TKI). Aprotinin is often used as a non-specific serine protease inhibitor, especially for inhibition of Trypsin.
Effect:Aprotinin inhibits proteases such as Trypsin, Plasmin, Chymotrypsin and Thrombin. Aprotinin is a competitive inhibitor of serine proteases that forms stable complexes with the enzyme and blocks active enzyme sites. These bindings are reversible and most aprotinin-protease complexes dissolve at very low or high pH values (<3 and >10).Application:Aprotinin is mainly used as a trypsin inhibitor. Stability:
Aprotinin can be stored in lyophylised form at +2°C to +8°C for an unlimited period of time. In salt or buffer solutions (pH 5 - 8), Aprotinin is stable for at least one month at +2°C to +8°C or for several years at -20°C. Stock solutions (e.g. 10mg/mL) should be frozen in small aliquots and used only once to avoid repeated freezing and thawing. Unit definition:One trypsin inhibitory unit (TIU) inhibits the activity of 2 trypsin units by 50%, whereby one trypsin unit hydrolyses 1 μmol Nα-benzoyl-DL-arginine-p-nitroanilide (BAPNA) per minute at pH 7.8 and 25°C. 1 TIU (trypsin inhibitory unit) corresponds to approx. 1300 KIU (kallikrein inhibitory units) and 1 TIU corresponds to one Ph. Eur. unit.
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ATP (Adenosine 5'-Triphosphate), disodium salt
Adenosine triphosphate (ATP) is the universal and immediately available energy source in cells and an important regulator of energy providing processes. The molecule adenosine triphosphate consists of an adenine, the sugar ribose and three phosphate (α to γ) in ester (α) or anhydride bond (β and γ). It was shown that ATP has an inhibitory effect on the inflammatory response by abrogating secretion of TNF-α and promoting secretion of Interleukin-10.
Synonyms: ATP, Adenosin-5'-Triphosphat.
Also available from Genaxxon: NADH Na salt (reduced) >.
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BCIP, molecular biology grade
BCIP (5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt) is a chromogenic substrate for alkaline phosphatase, used in combination with the oxidant NBT (nitro blue tetrazolium) to enhance blue color development.
Applications: When alkaline phosphatase conjµgates are used for detection, BCIP is recommended for the following applications: Southern blotting, Western blotting, Northern blotting and dot/slot blots.
It is recommended to prepare a 50mg/mL stock solution in DMF.Protocol for Preparation of BCIP/NBT Substrate Solution:Add the following components- 10mL of the Alkaline Phosphatase (AP) buffer (100mM Tris-HCl (pH9.5), 100mM NaCl and 10mM MgCl2)- 33µL BCIP (50mg/mL in DMF) - 44µL NBT (75mg/mL in 70% DMF).
NOTE: Avoid exposure to light - Prepare fresh developing solution and use within an hour - BCIP and NBT solutions in DMF do not freeze. They are stable for approximately 2 years when stored at -20°C in the dark.
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BES (buffer quality)
BES (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) is a useful secondary standard biochemical buffer. Useful pH range for BES is 6.4 to 7.8. It is useful for diagnostic assay manufacturing industry. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.
Synonyms: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid; N,N-Bis(2-hydroxyethyl)taurine.
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Bestatine Hydrochloride
Bestatin is a potent aminopeptidase inhibitor. The compound has multiple physiological functions including the ability to act as an immunomodifier and enhance the proliferation of human bone marrow granulocyte-macrophage progenitor cells to form CFU-GM colonies. Research indicates that bestatin can indirectly act on monocytes by inhibiting Aminopeptidase B, which in turn inhibits the catabolism of tuftsin. Bestatin has also been shown to inhibit Leukotriene A4 hydrolase (LTA4H). This compound has been demonstrated to bind to cell surfaces and research indicates that it can directly stimulate lymphocytes through its fixation on the cell surface. The agent has been used in multiple leukemia studies. Synonym: N-[(2S,3S)-3-Amino-2-hydroxy-4-phenyl-butyryl]-L-Leucine hydrochloride.
You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.
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beta-Nicotinamide adenine dinucleotide - beta-NAD
Nicotinamide adenine dinculeotide, min. 95% (HPLC). Suitable for cell culture.Synonym: β-DPN; β-NAD, oxidised form; Coenzyme 1; Cozymase; DPN; Diphosphopyridine nucleotide; Nadide; b-Nicotinamide adenine dinucleotide; b-NAD.
Used, among other things, for oxidation of malate. Composition of the measuring buffer for the oxidation of malate: 100mM Tris HCl (pH8), 10mM L-Malat, 0,5mM NAD, 20mM KCl and 1mM MnCl2.
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Bicine buffer grade
Bicine is recommended for low temperature biochemical work and for the preparation of stable substrate solution for serum guanase determination. Bicine is used for different applications, ranging from buffers used in enzymatic reactions to electrophoretic buffers. Bicine can not be used for BCA and Lowry protein determination procedures. Working concentrations: 3 mM up to 100 mM.
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Bis-Tris, p.A.
2-[Bis(2-hydroxyethyl)imino]-2-(hydroxymethyl)-1,3-propandiol. Puffersubstanz: Daboo M. and Bates R. (1970) J. Phys. Chem., 74, 702-5.
Bis-Tris is an amino buffer very similar in its chemical structure to Trizma ("Tris"). Bis-Tris is a frequently used important buffer if working with proteins or nucleic acids. Bis-Tris is also used very often zu replace buffers that contain Cacodylic acid (Dimethylarsinic acid).
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Bluo-Gal / 5-Bromo-3-indolyl-beta-D-galactopyranoside
5-Bromo-3-indolyl-β-D-galactopyranosid (Bluo-Gal) ist used as a substrat of β-Galactosidase. It is a chromogenic substrate suitable for identification of lacZ+ bacterial colonies. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
PrincipleWhen Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.
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Calcium lactate pentahydrate
Calcium lactate pentahydrate - Lactic acid calcium salt
Synonyms: L-Lactic acid calcium salt; Calcium L-lactate pentahydrate; Calcium L-lactate pentahydrate; Calcii lactas pentahydricus; L-Lactic acid calcium salt; Calcium lactate hydrate; Calcium L-lactate hydrate; L-Lactic acid calcium salt hydrate
CAPS >99% pure - buffer grade
3-(Cyclohexylamino)-1-propane sulfonic acid
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Carbonate-Bicarbonate buffer tablets (pH9.6)
1 tablet dissolved in 100mL of deionized water yields: 0.05M Sodium Carbonate-bicarbonate buffer, pH9.6 at 25°C.
No time consuming and expenisve calibration / pH-measuring necessary. Just dissolve buffer talets in water and use buffer solution.
You will find more ready-to-use buffers here: ready-to-use buffers >
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Carbonate-Bicarbonate buffer tablets with Azid (pH9.6)
1 tablet dissolved in 100mL of deionized water yields: 0.05M Sodium Carbonate-bicarbonate buffer, 0.05% Sodium Azide, pH9.6 at 25°C.
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CHAPS buffer grade
CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry, isoelectric focusing and two-dimensional electrophoresis. Useful for solubilizing membrane proteins and breaking protein-protein interactions. Its small micellar molecular weight (6150) and high critical micellar concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilisation for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Commonly used concentrations for IEF are 2-4% (v/v).
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CHAPS for 2D-gel electrophoresis
CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry, isoelectric focusing and two-dimensional electrophoresis. Useful for solubilizing membrane proteins and breaking protein-protein interactions. Its small micellar molecular weight (6150 Da) and high critical micellar concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilisation for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Commonly used concentrations for IEF are 2-4% (v/v).
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![CHAPSO; 3-([3-Cholamidopropyl]dimethylammonio)-2-hydroxy-1-propanesulfonate](https://www.genaxxon.com/media/e0/8a/9b/1708287096/m6202_chapso.jpg?ts=1708287096 "m6202_chapso")
CHAPSO - min. 99.0% (HPLC)
3-[(3-Cholamidopropyl)-dimethylammonio]-2-hydroxy-1-propane sulfonate
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Tip
CHES, min. 99.0%
CHES shows a pKa (25°C) of 9,55 which makes CHES useful as a buffering component in the pH range between 8.6 to 12.0. CHES interferes in the protein quantification according to Lowry.
It is used in crystallisation of Phosphotriesterase (50mM) or in the chemical modification of Bacteriorhodopsin (10mM). Synonyms are: 2-(Cyclohexylamino)ethanesulfonic acid; 2-(N-Cyclohexylamino)ethanesulfonic acid.
Other buffers are: BES, BICIN, MOPS, CAPS, CHAPS
Citric acid trisodium salt dihydrate, p.A. min. 99.0%
Citric acid trisodium salt (tri-sodium citrate, sodium citrate) is used as a buffering substance for molecular biology buffers.
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Coenzyme A trilithium salt (>93%)
Coenzyme A (CoA, CoASH, HSCoA) is a coenzyme that facilitates enzymatic acyl-group transfer reactions and supports the synthesis and oxidation of fatty acids. CoA is involved in the mechanisms of a wide variety of enzymes.
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Colcemid - 10mL
Colcemid inhibits the formation of mitotic spindles. It is used to increase the percentage of metaphase cells for chromosome analysis. Colcemid depolymerizes microtubules; blocks mitosis at metaphase. Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.
You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.
Collagenase-Chromophore-Substrate Component A
Collagenase-Chromophore-Substrate Component A also named 4-Phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg-OH x 2H2O.
D-Galactosamine x HCl
Galactosamine is a hexosamine derived from galactose with the molecular formula C6H13NO5. This amino sugar is a constituent of some glycoprotein hormones such as follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Other sugar constituents of FSH and LH include glucosamine, galactose and glucose.
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D-Glucosamine x HCl, min. 99% (HPLC)
CAS: [66-84-2] - C6H13NO5 x HCl
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D-Glucose-6-phosphate disodium
Glucose 6-phosphate (also known as Robison ester) is glucose sugar phosphorylated on carbon 6. This compound is very common in cells as the vast majority of glucose entering a cell will become phosphorylated in this way. Within a cell, glucose 6-phosphate is produced by phosphorylation of glucose on the sixth carbon. This is catalyzed by the enzyme hexokinase in most cells, and, in higher animals, glucokinase in certain cells, most notably liver cells. One molecule of ATP is consumed in this reaction.
Synonyms: G-6-P-Na2; Robison ester; Robison ester disodium salt; G6P-Na2.
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€446.25
D-Luciferin potassium salt
D-Luciferin also named Firefly Luciferin is the most popular and versatile bioluminescent substrate. Firefly luciferase produces light by the ATP-dependent oxidation (Mg2+ as cofactor) of luciferin. It emits a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase activity when luciferin and ATP are present in excess. Firefly luciferase has long been conjugated to antibodies and used as a label in immunoassays using luciferin as the substrate for detection. One particular advantage to the enzyme is that there is low endogenous luciferase activity in mammalian tissues besides its high sensitivity. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain. Luciferin is a common reagent used throughout the biotechnology field and specifically for in vivo imaging. Luciferase labeled tumor cells, stem cells or infectious diseases are often inoculated into research animals such as rats or mice for investigation. The injection of luciferin allows for the real-time, non-invasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI). Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays (e.g., determine cell viability and bacteria counting).
Variants from €96.53
Regular price:
€369.06
D-Mannitol, min. 98% (HPLC)
D-Mannitol. Purity >99.3%, CAS: [69-65-8] - C6H14O6
Variants from €60.08
Regular price:
€119.59
D-Mannose, min. 99% (HPLC)
CAS: [3458-28-4] - C6H12O6
Variants from €43.27
Regular price:
€132.10
Variants from €43.27
Regular price:
€468.72
Variants from €43.27
Regular price:
€276.41
D-Raffinose pentahydrate (min. 98% HPLC)
D-Raffinose also known as O-α-D-Galactopyranosyl-(1→6)-α-D-glucopyranosyl β-D-fructofuranoside; Melitose; Melitriose; Melitose pentahydrate.
Variants from €74.52
Regular price:
€156.02
D.(+)-Galacturonic acid monohydrate (>98% by titration)
Galacturonic acid (GalA) is the primary building block of mainly pectin but also other biopolymers found in plants. The polymeric GalA chains in pectin, are linked by alpha-1,4 glycosidic bonds. Some of the carboxyl groups are methylated and exist as methyl esters. The molecular mass of pectins reaches 200,000 g/mol and more.
Pectin is found mainly in plant walls. It is particularly abundant (≈30 wt%) in citrus peels, but is also extracted from apples, spinach, sugar beets, and other fruits and vegetables. It is mainly used as a gelling or filling agent in foods such as jellies, jams, desserts and sweets, and as a stabilizer in juices and milk-based beverages.
Variants from €204.14
Regular price:
€1,098.19
Variants from €204.14
Regular price:
€314.67
D(+)-Fucose (6-Deoxy-D-galactose), min. 99%
D-Fucose (6-Deoxy-D-galactose) may be used for different purposes.
1. In studies of fucoidan polysaccharide containing glycans.2. D-Fucose is used as a substrate to identify, differentiate and characterize enzymes such as the fucosidase(s), l-fucose isomerase(s) and L-fucose dehydrogenase(s).3. D-Fucose may be used to study organelles, bacterial microcompartments, involved in the degradation of plant and algal cell wall sugars.4. D-Fucose may be used as a reference compound in rare sugar identification and analysis.
Variants from €88.64
Regular price:
€147.72
Variants from €88.64
Regular price:
€596.80