Biochemicals and Biochemistry Products

TLCK is an irreversible, specific inhibotor of the Serin-protease Trypsin and inhibits the Thrombin-like protease Cerastocytin. Together with the competitive inhibitors benzamidin hydrochlorid or 8M urea Trypsin does not show inhibitiory effects. Chymotrypsin is not inhibited by TLCK. The pH optimum for inhibition through Histidin modification is pH 7.5. Chymotrypsinogen is not inhibited by TLCK. A concentration of 50 µg/mL of TLCK inhibits 100% of the Trypsin like proteinase activity of wheat seat extracts. Besides proteases also protein kinases are inhibited by TLCK. Obviously by the modification of the enzymatic activity centre of the protein kinases. TLCK solutions are most stable at pH 6.0 at +4°C. TLCK is not stable at pH above 7.0. Therefore stock solutions of 1 mg/mL to 20 mg/mL are prepared with buffer solutions of pH below 6.0. Always pipete shortly before the inhibition experiment. Working solutionsa are: 50 µg/mL to 100 µg/mL. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.5M Tris buffered saline, 1.38M NaCl, 0.027M KCl, pH8.0 at 25°C.

Collagenase-Chromophore-Substrate Component A also named 4-Phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg-OH x 2H2O.

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.89M Tris-borate, 0.02M EDTA, pH8.3 at 25°C. In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis.TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels. TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide. Medicago’s TBE and TAE buffers are supplied as a pre-weighed powder mix in sealed pouches giving 1000mL of 1X, 5X or 10X Tris-borate-EDTA buffer or 50X Tris-acetate-EDTA buffer with pH 8.3 at 25°C. You will find more ready-to-use buffers here: ready-to-use buffers >

Ready-to-use 100mM IPTG solution (Isopropyl-ß-D-thiogalactopyranoside) for molecular biology research. IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used together with X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactoside) or Bluo-Gal (5-Bromo-3-indolyl-beta-D-galactopyranoside) in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. While IPTG binds to the lacI repressor it is altering the conformation of the lacl repressor, which prevents the repression of the β-galactosidase coding gene lacZ. Thus IPTG is an inducer of the lac operon in bacteria for detection of lac+ colonies. Shipped as 10 x 1mL solution.

a-D-Mannose-1-phosphate dipotassium salt dihydrate (synthetic crystalline product). a-D-Mannopyranose-phosphate. Free carbonate and phosphate are completely removed. Product shipped with certificate covering all data for validation (NMR, FTIR, GC, GC-MS, AAS, HPLC, optical rotation and MP. Man-1-P.

Synonyms: D-threo-pent-2-ulose. Xylulose a ketopentose, is a monosaccharide containing five carbon atoms, and including a ketone functional group. In nature, it occurs in both the L- and D-enantiomers. L-Xylulose accumulates in the urine in patients with pentosuria, due to a deficiency in L-xylulose reductase. Since L-xylulose is a reducing sugar like D-glucose, pentosuria patients have been wrongly diagnosed in the past to be diabetic.

Contents of 1 pouch dissolved in deionized water and made up to 500mL/1000mL yields: 2.0M Tris acetate buffer, 0.05M EDTA, pH8.3 at 25°C. In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis.TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels. TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide. Medicago’s TBE and TAE buffers are supplied as a pre-weighed powder mix in sealed pouches giving 1000mL of 1X, 5X or 10X Tris-borate-EDTA buffer or 50X Tris-acetate-EDTA buffer with pH 8.3 at 25°C.

Aphidicolin inhibits the growth of eukaryotic cells and certain animal viruses by selectively inhibiting the cellular replication of DNA polymerase II or the viral-induced DNA polymerases. The drug may be useful for controlling excessive cell proliferation in patients with cancer, psoriasis or other dermatitis with little or no adverse effect upon non-multiplying cells. Aphidicolin is a tool in cell proliferation and differentiation research. Aphidicolin may possibly be used for controlling excessive cell proliferation in cancer, psoriasis or other dermatitis with little or no adverse effect upon non-multiplying cells. Synonyms:(3-a,4-a,5-,17-a)-3,17-Dihydroxy-4-methyl-9,15-cyclo-C,18-dinor14,15-secoandrostane-4,17-dimethanole. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Ready-to-use PBS tablets, individually packed in a PE bag. Just dissolve 1 of  the PBS tablets in 1000mL  deionized water  to yield a 0.01M Phosphate buffer, 0.0027M KCl, 0.14M NaCl, pH7.4 at 25°C. No time consuming weighing or measuring of pH necessary anymore. You can get rid of the whole validation process and you will have no need for scales or pH-meters anymore. Amoung biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns. Our PBS is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches. There are different standard-sized packages and final volumes range from 100mL to 100 litres. Choose from different pH (7.2 or 7.4), from PBS with our without potassium, or with and without Tween®. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >).PBS with Tween™20, pH7.4 (D2003 (100mL and 500mL) > and (D2004 (1L) >).PBS, pH7.2 (D2034 >) PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

Etoposide phosphate (Eposin®, Etopophos®, Vepesid®, VP-16®) is an inhibitor of the enzyme topoisomerase II. For medical purposes it is used as a form of chemotherapy for malignancies such as lung cancer, testicular cancer, lymphoma, non-lymphocytic leukemia, and glioblastoma multiforme. It is often given in combination with other drugs. Chemically it derives from podophyllotoxin, a toxin found in the American Mayapple. Genaxxon offers this product only for in-vitro (laboratory) usage! You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

X-alpha-D-Gal is a chromogenic substrate for alpha-galactosidase yielding a blue precipitate (X-alpha-Gal is a chromogenic substrate for yeast galactosidase (MEL1) and is used for detecting GAL4-based yeast two-hybrid interactions directly on agar). Used for species differentiation within the family Enterobacteriaceae and differentiation of Bifidobacteria species from Lactobacillus species (see references). Synonmys: 5-Bromo-4-chloro-3-indolyl a-D-galactopyranoside, X-α-Gal; X-alpha-Gal. Other products that are used as a substrate for alpha or beta-Galactosidase: M3225: 5-Bromo-3-indolyl-beta-D-galactopyranoside >M3204: 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal) >M3205: 5-Bromo-4-chloro-3-indolyl-ß-D-glucopyranoside (X-Glu / X-Glc) > References: 1. Aho S., Arffman A., Pummi T., Uitto J., A novel reporter gene MEL1 for the yeast two-hybrid system. Anal Biochem. 1997, 253, 270-2. 2. Chevalier P., et al., X-alpha-Gal-base medium for simultaneous enumeration of bifidobacteria and lactic acid bacteria in milk. J. Microbiol. Methods 1991, 13, 75. 3. Gossrau R., Lojda Z., Histochemical detection of alpha-D-galactosidase with 5-Br-4-Cl-3-indoxyl alpha-D-galactoside. Acta Histochem. 1989, 85, 213-22. 4. Perry J.D., Ford M., Taylor J., Jones A.L., Freeman R., Gould F.K., ABC medium, a new chromogenic agar for selective isolation of Salmonella spp. J. Clin. Microbiol. 1999, 37, 766-8.

pNPh-a-D-Man, CAS: [10357-27-4] - C12H15NO8

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 1M Tris-HCl buffer, pH7.4 at 25°C. Sterilization can be performed by filtration. Filtrate the buffer solution through a 0.22µm filter into a sterile flask. Keep the buffer solution at +2°C to +8°C. The pH value of a Tris buffer strongly depends on the temperature. The pKa of 8.06 changes approximately 0.03 units per degree Celsius.

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 1M Tris-HCl buffer, pH8.3 at 25°C. Sterilization can be performed by filtration. Filtrate the buffer solution through a 0.22µm filter into a sterile flask. Keep the buffer solution at +2°C to +8°C. The pH value of a Tris buffer strongly depends on the temperature. The pKa of 8.06 changes approximately 0.03 units per degree Celsius.

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 1M Tris-HCl buffer, pH8.0 at 25°C. Sterilization can be performed by filtration. Filtrate the buffer solution through a 0.22µm filter into a sterile flask. Keep the buffer solution at +2°C to +8°C. The pH value of a Tris buffer strongly depends on the temperature. The pKa of 8.06 changes approximately 0.03 units per degree Celsius.

TPCK is an irreversible, specific inhibotor for chymotrypsin and inhibits the thrombin-like protease Cerastocytin. Trypsin is not inhibited by TPCK. The pH optimum for inhibition throµgh alkylation by TPCK of Chymotrypsin is pH7.2. Chymotrypsinogen is not inhibited by TPCK. The bacterial Serin-proteases Re, Fa and So from E.coli are completely inhibited by 0.5 to 1 mM TPCK. TPCK is used for the production of Chymotrypsin free Trypsin. TPCK solutions are stable at pH below 7.0 at RT, nevertheless it is recommended to store solutions at +2°C to +8°C. TPCK is not stable at pH above 7.0. Stock solutions of 3mg/mL to 20mg/mL are prepared in MeOH or EtOH. Working concentrations: 10µg/mL to 100µg/mL. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.445M Tris-borate, 0.01M EDTA, pH8.3 at 25°C. In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis. TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels. TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide. Medicago’s TBE and TAE buffers are supplied as a pre-weighed powder mix in sealed pouches giving 1000mL of 1X, 5X or 10X Tris-borate-EDTA buffer or 50X Tris-acetate-EDTA buffer with pH 8.3 at 25°C. You will find more ready-to-use buffers here: ready-to-use buffers >

pNPh-a-L-Fuc, CAS: [22153-71-5] - C12H15NO7

Precisely weighed ready-to-use PBS buffer tablets offered in plastic bottles or in practical blister packs. - no awkward weighing anymore- no more time-consuming pH adjustment- simply dissolve the tablet completely in demineralized water and use the buffer 1 tablet dissolved in 500mL of deionized water yields: 0.01M Phosphate buffer, 0.0027M KCl, 0.14M NaCl, pH7.4 at 25°C. Amoung biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns. Our PBS is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches. There are different standard-sized packages and final volumes range from 100mL to 100 litres. Choose from different pH (7.2 or 7.4), from PBS with our without potassium, or with or without Tween. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >). PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

AEBSF is an irreversible inhibitor of Thrombin and other Serin proteases (Chymotrypsin, Kallikrein, Plasmin, Proteinase K, Trypsin) by sulfonylation of a functional group in the active centre of the enzyme. AEBSF is much less toxic compared to PMSF and DFP and therefore recommended to substitute these reagents. AEBSF is highly soluble in water resulting in a solution of pH 6.5. At this pH AEBSF is most stable. We recommend to store stock solutions (20 mM or 100 mM in water or buffer) at -20°C for up to 2 months and prepare fresh working solutions. AEBSF is used at a working concentration of 0.1 - 0.2 mM. Walsmann, P. et al. (1972) Acta biol. med. germ. 28, 577-585, Nathan, D.F. & Linquist, S. (1995) Mol. Cell. Biol., 15, 3917-3925. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

a-D-Galactose-1-phosphate dipotassium salt dihydrate (synthetic crystalline product). Free carbonate and phosphate are completely removed. a-D-Galactose-1-phosphate. Product shipped with certificate covering all data for validation (NMR, FTIR, GC, GC-MS, AAS, HPLC, optical rotation and MP. Gal-1-P.

Content of 1 pouch dissolved in deionized water and made up to 500mL/1000mL yields: 0.5M EDTA, pH8.0 at 25°C. EDTA (ethylene-diamine-tetraacetic acid) is a chelating agent widely used in molecular biology to sequester divalent and trivalent metal ions such as calcium and magnesium. This ability prevents DNA and RNA degradation as metal-dependent enzymes acting as nucleases become deactivated. A fully deprotonated EDTA molecule will bind directly to the metal ion making the buffer suitable for adding to stored blood as an anti-coagulant to bind Ca2+ ions. Furthermore, EDTA is useful for cell culture procedures as it prevents clumping of cells in liquid suspension and detaches adherent cells when passaging. Genaxxon’s EDTA buffer is supplied in two sizes with pouches giving 500mL or 1000mL of 0.50M EDTA buffer with pH8.0 at 25°C when the contents of one pouch is dissolved in deionized water.

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 1M Sodium phosphate buffer, pH6.5 at 25°C. Other phosphate-based ready-to-use buffers:: PBS without Potassium (0.01M) (D2033 >). PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 1M Sodium phosphate buffer, pH7.2 at 25°C. Other phosphate-based ready-to-use buffers:: PBS without Potassium (0.01M) (D2033 >).PBS with Tween™20, pH7.4 (D2003 (100mL and 500mL) > and (D2004 (1L) >).PBS, pH7.2 (D2034 >) PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

ready-to-use PBS buffer tablets. Each tablet dissolved in 1000mL of deionized water yields: 0.01M Phosphate buffer, 0.0027M KCl, 0.14M NaCl, pH7.4 at 25°C. No time consuming weighing or measuring of pH necessary anymore. You can get rid of the whole validation process and you will have no need for scales or pH-meters anymore. Amoung biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns. Our PBS is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches. There are different standard-sized packages and final volumes range from 100mL to 100 litres. Choose from different pH (7.2 or 7.4), from PBS with our without potassium, or with and without Tween®. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >). PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

Precisely weighed ready-to-use PBS tablets with Tween®20 offered in plastic bottles or in practical blister packs. - no awkward weighing anymore- no toilsome pipetting/weighing of Tween®20 anymore- no more time-consuming pH adjustment- simply dissolve the tablet completely in demineralized water and use the buffer Ready-to-use PBS buffer tablets with Tween®20 containing the non-ionic detergent Tween®20 which acts through blocking. It has the ability to reduce non-specific binding and protein-protein interaction during the wash step in protein and immunoassay procedures such as ELISA and Western blotting. By decreasing the non-specific binding and staining makes interpretation of ELISA results and Western blots easier. Each tablet dissolved in deionized water yields a 0.14M sodium chloride 0.0027M potassium chloride, 0.05% Tween®20, 0.01M phosphate buffer with pH7.4 at 25°C. Final buffer volumes are: 100mL and 500mL (D2003 >) or 1L (D2004 >). Our ready-to-use tablets are also available as PBS tablets without Tween®20, as TBS or as IMAC tablets for affinity chromatography. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >). PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

pNPh-b-D-GlcNAc - CAS: [3459-18-5] - C14H18N2O8

10 bags of a 10-fold Tris-EDTA buffer (pH7.4) - ready-to-use Tris/HCl-EDTA powder mixture for 1L ready-to-use buffer solution of pH7.4. This TE buffer is composed by Tris, a buffering agent and EDTA. EDTA has the ability to prevent degradation of DNA and RNA by chelating magnesium- or other divalent metal ions.Tris-EDTA-based solutions break protein cross-links and can therefore unmask antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections. Treatment with TE buffer enhances the staining intensity of antibodies in the immuno-histochemical detection of certain proteins. TE buffer powder is supplied as exactly pre-weighed powder in pouches. To prepare the buffer, the content of a bag is simply dissolved in 1L of deionized water. This results in a 0.1M Tris/HCl, 0.01M EDTA buffer of pH7.4 at 25°C.       T

Galacturonic acid (GalA) is the primary building block of mainly pectin but also other biopolymers found in plants. The polymeric GalA chains in pectin, are linked by alpha-1,4 glycosidic bonds. Some of the carboxyl groups are methylated and exist as methyl esters. The molecular mass of pectins reaches 200,000 g/mol and more. Pectin is found mainly in plant walls. It is particularly abundant (≈30 wt%) in citrus peels, but is also extracted from apples, spinach, sugar beets, and other fruits and vegetables. It is mainly used as a gelling or filling agent in foods such as jellies, jams, desserts and sweets, and as a stabilizer in juices and milk-based beverages.

ready-to-use PBS buffer tablets. Each tablet dissolved in 1000mL of deionized water yields: 0.01M Phosphate buffer, 0.0027M KCl, 0.14M NaCl, pH7.2 at 25°C. No time consuming weighing or measuring of pH necessary anymore. You can get rid of the whole validation process and you will have no need for scales or pH-meters anymore. Amoung biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns. Our PBS is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches. There are different standard-sized packages and final volumes range from 100mL to 100 litres. Choose from different pH (7.2 or 7.4), from PBS with our without potassium, or with and without Tween®. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >). PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

pNPh-b-D-Xyl - CAS: [2001-96-9] - C11H13NO7

E-64 is an irreversible inhibitor of different cysteine proteases (papain, bromelain, ficin, cathepsin B, H and L, tumor-cathepsin, calpain, V-CATH from Autographa californica M nucleopolyhedro virus (AcMNPV) and Streptococcus-protease). All enzymes are completely inhibited by a concentration of 10 - 30 μM within 30 minutes. E-64 and other protease inhibitors (e. g. diisopropylfluorophosphate and PMSF) are able to inhibit the programmed cell death, that is induced by activation of the T cell receptor in different cell lines of the immune system, not by blocking the signal transduction. The effect of dexamethason, which induces apoptosis as an antagonist of the T cell receptor, is enhanced. If proteins are expressed in the baculo virus system with a functional v-cath gene, very often a proteolytic activity can be observed. This reduces the quality of the expressed protein significantly. The addition of E-64 to all reaction steps avoids the degradation almost completely. Stability: E-64 is stable against reducing agents. Stock solutions have concentrations between 1mM and 10mM in DMSO or 50% ethanol and are stable for several days, if stored at +4°C. Aliquots may be stored at -20°C for approx. one month. Synonyme: trans-Epoxysuccinyl-L-leucylamido(4-guanidino)butan; L-trans-3-Carboxyoxiran-2-carbonyl-L-leucylagmatin; N-(trans-Epoxysuccinyl)-L-leucin-4-guanidinobutylamid You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

5-Bromo-4-chloro-3-indolyl b-D-glucuronide cyclohexylammonium salt is a Glucuronidase substrate. X-Gluc is cleaved by Glucuronidase into two molecules, glucuronic acid and 5-bromo-4-chloro-indoxyle. 5-bromo-4-chloro-indoxyle is then being oxidized by oxigen to a deep-blue dye (Indigo) which is insoluble. X-Gluc is used for the detection of E. coli bacteria in food, water and feed. In molecular biology, it is often used as a marker for analysing the expression of target genes. Synonyms: X-glucuronide CHA salt; X-GlcA; 5-Bromo-4-chloro-3-indoxyl beta-D-glucuronide cyclohexylammonium salt; X-glucuronide CHA salt; 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt.

Rose-Gal, also known as Red-Gal or Salmon-Gal, (6-Chloro-3-indolyl-ß-D-galactopyranoside), is a is a beta-galactosidase substrate which produces a salmon pink colour. Rose-Gal (Red-Gal / Salmon-Gal) serves as an alternative to X-Gal > for the enzymatic detection of Lac+ beta-Galactosidase activity in bacterial colonies. In colorimetric assays, recombinants (white) can be distinguished from non-recombinants (salmon). In conjunction with X-Gluc >, Rose-Gal (Red-Gal, Salmon-Gal) can be used for the simultaneous detection of GUS and Lac + activity on the same plate.

Substrate for horseradish peroxidase detection assays.

n-Tridecyl-ß-maltoside (TDM) >99.5% for crystallography.

Precisely weighed ready-to-use PBS buffer tablets offered in plastic bottles or in practical blister packs. - no awkward weighing anymore- no more time-consuming pH adjustment- simply dissolve the tablet completely in demineralized water and use the buffer 1 tablet dissolved in 500mL of deionized water yields: 0.01M Phosphate buffer, 0.0027M KCl, 0.14M NaCl, pH7.4 at 25°C. Amoung biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns. Our PBS is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches. There are different standard-sized packages and final volumes range from 100mL to 100 litres. Choose from different pH (7.2 or 7.4), from PBS with our without potassium, or with or without Tween. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >). PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

Coenzyme A (CoA, CoASH, HSCoA) is a coenzyme that facilitates enzymatic acyl-group transfer reactions and supports the synthesis and oxidation of fatty acids. CoA is involved in the mechanisms of a wide variety of enzymes.

Bestatin is a potent aminopeptidase inhibitor. The compound has multiple physiological functions including the ability to act as an immunomodifier and enhance the proliferation of human bone marrow granulocyte-macrophage progenitor cells to form CFU-GM colonies. Research indicates that bestatin can indirectly act on monocytes by inhibiting Aminopeptidase B, which in turn inhibits the catabolism of tuftsin. Bestatin has also been shown to inhibit Leukotriene A4 hydrolase (LTA4H). This compound has been demonstrated to bind to cell surfaces and research indicates that it can directly stimulate lymphocytes through its fixation on the cell surface. The agent has been used in multiple leukemia studies. Synonym: N-[(2S,3S)-3-Amino-2-hydroxy-4-phenyl-butyryl]-L-Leucine hydrochloride. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Widely used in molecular biology experiments for the preparation of prehybridisation and hybridisation solutions, wash solutions, etc.. The pH value of the dissolved SSC buffer powder is approx. 8.2 ± 0.2. Adjust pH to 7.0 ± 0.2 with HCl.

Acetyl Coenzyme A (Ac CoA) is the end product of glycolysis and takes part in the Ac CoA pathway, which is a metabolic pathway for carbon compounds. Ac-CoA is important in cholesterol synthesis. Fatty acids are relatively unreactive and must therefore be activated before reactions with coenzyme A. The key enzyme is the Acyl CoA synthetase, also called thiokinase. The fatty acid is first formed by the thiokinase (attachment of AMP) to acyl adenylate. Acyl adenylate reacts under the cleavage of AMP and the addition of CoA (catalyzed by the thiokinase) to form Acyl CoA. Accordingly, acetic acid can also be activated and forms Acetyl CoA. Concentration and stabilityWorking solution: Optimal solvent: water or aqueous solution with a weak acidic pH (4 to 5).Storage conditions of the working solution: -15°C to -25°C.A solution of 50mg/mL in phosphate buffer, pH7, is stable for 3 weeks at -15°C to -25°C. Unfrozen solutions should be used immediately.

Urea powder preweight and packed in aluminium foil. ready-to-use for preparing 100mL of a 5M urea solution.

Technical data can be found under Detail view.

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry, isoelectric focusing and two-dimensional electrophoresis. Useful for solubilizing membrane proteins and breaking protein-protein interactions. Its small micellar molecular weight (6150 Da) and high critical micellar concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilisation for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Commonly used concentrations for IEF are 2-4% (v/v).

2-[Bis(2-hydroxyethyl)imino]-2-(hydroxymethyl)-1,3-propandiol. Puffersubstanz: Daboo M. and Bates R. (1970) J. Phys. Chem., 74, 702-5. Bis-Tris is an amino buffer very similar in its chemical structure to Trizma ("Tris"). Bis-Tris is a frequently used important buffer if working with proteins or nucleic acids. Bis-Tris is also used very often zu replace buffers that contain Cacodylic acid (Dimethylarsinic acid).

Description:Aprotinin is mainly obtained from bovine lung. Aprotinin consists of 58 amino acids of 6511 Da and is very basic, whereby amino acids 13-18 of Aprotinin are of particular importance for binding to serine proteases. As Aprotinin has been described by various research groups, two names have become established: 1. bovine pancreatic trypsin inhibitor (BPTI) and 2. trypsin-kallikrein inhibitor (TKI). Aprotinin is often used as a non-specific serine protease inhibitor, especially for inhibition of Trypsin. Effect:Aprotinin inhibits proteases such as Trypsin, Plasmin, Chymotrypsin and Thrombin. Aprotinin is a competitive inhibitor of serine proteases that forms stable complexes with the enzyme and blocks active enzyme sites. These bindings are reversible and most aprotinin-protease complexes dissolve at very low or high pH values (<3 and >10).Application:Aprotinin is mainly used as a trypsin inhibitor. Stability: Aprotinin can be stored in lyophylised form at +2°C to +8°C for an unlimited period of time. In salt or buffer solutions (pH 5 - 8), Aprotinin is stable for at least one month at +2°C to +8°C or for several years at -20°C. Stock solutions (e.g. 10mg/mL) should be frozen in small aliquots and used only once to avoid repeated freezing and thawing. Unit definition:One trypsin inhibitory unit (TIU) inhibits the activity of 2 trypsin units by 50%, whereby one trypsin unit hydrolyses 1 μmol Nα-benzoyl-DL-arginine-p-nitroanilide (BAPNA) per minute at pH 7.8 and 25°C. 1 TIU (trypsin inhibitory unit) corresponds to approx. 1300 KIU (kallikrein inhibitory units) and 1 TIU corresponds to one Ph. Eur. unit.

The Prestained Protein Marker - 10-245 kDa is a three-color protein standard with 12 pre-stained (covalently coupled to a chromophore) purified proteins. Proteins are covalently coupled with a blue chromophore except for two reference bands, one green at 25 kDa and one red one at 75 kDa respectively, when separated on SDS-PAGE (Tris-glycine buffer). The protein bands represent 10 kDa, 15 kDa, 20 kDa, 25 kDa (green), 35 kDa, 45 kDa, 60 kDa, 75 kDa (red), 100 kDa, 140 kDa, 180 kDa and 245 kDa. The Prestained Protein Marker 10-245 kDa is delivered in convenient 2 x 250µL aliquots. With a recommended application load of 3-5μL per lane, the marker lasts for up to 100 gels.

PEG 1000, Polyglycol 1000, HO(C2H4N)nH

Nicotinamide adenine dinucleotide phosphate reduced - Tetrasodium salt in short NADPH is the reduced version of NADP+ and can transfer a hydride atom to another reaction partner in a redox reaction. The abbreviations NADP+ for the oxidized form, NADPH for the reduced form and NADP in general are proposed by IUPAC. β-Nicotinamide adenine dinucleotide 2'-phosphate (NADP +) and β-nicotinamide adenine dinucleotide 2'-phosphate, reduced (NADPH), comprise a coenzyme redox couple (NADP+ : NADPH) involved in a variety of enzyme-catalyzed oxidation-reduction reactions. The NADP+ / NADPH redox couple facilitates electron transfer in anabolic reactions such as lipid and cholesterol biosynthesis and fatty acyl chain extension. The NADP+ / NADPH redox system is used in a variety of antioxidant mechanisms to prevent the accumulation of reactive oxidation products. NADPH is generated in vivo by the pentose phosphate pathway (PPP).

5-Bromo-3-indolyl-β-D-galactopyranosid (Bluo-Gal) ist used as a substrat of β-Galactosidase. It is a chromogenic substrate suitable for identification of lacZ+ bacterial colonies. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype. PrincipleWhen Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

TAPS (N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid) is used in biochemistry, molecular biology, microbiology and cell culture as buffer substance with optimal buffer range at pH7.7-9.1 (pKa = 8.4 (20°C). Hepes is one of the "goods buffers." Dr. Norman Good et al. descriped in his work in 1966 a selection of "biological buffers" that display similar characteristics. These characteristics include: pKa value between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbence between 240 nm and 700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations, and limited permeability of biological membranes. Goods buffers are: BES, BICINE, CAPS, EPPS, HEPES, MES, MOPS, PIPES, TAPS, TES, TRICINE. Reference:Good N.E. et al. (1966) Hydrogen Ion Buffers for Biological Research, Biochemistry 5, 467-77. Shipman jr. Ch. (1969) Proc. Soc. Exp. Biol. Med. 130, 305-10.

n-Decyl-b-maltoside (DM) >99.5% for crystallography

Calcium lactate pentahydrate - Lactic acid calcium salt Synonyms: L-Lactic acid calcium salt; Calcium L-lactate pentahydrate; Calcium L-lactate pentahydrate; Calcii lactas pentahydricus; L-Lactic acid calcium salt; Calcium lactate hydrate; Calcium L-lactate hydrate; L-Lactic acid calcium salt hydrate

The Prestained Protein Marker - 10-245 kDa is a three-color protein standard with 12 pre-stained (covalently coupled to a chromophore) purified proteins. Proteins are covalently coupled with a blue chromophore except for two reference bands, one green at 25 kDa and one red one at 75 kDa respectively, when separated on SDS-PAGE (Tris-glycine buffer). The protein bands represent 10 kDa, 15 kDa, 25 kDa (green), 35 kDa, 45 kDa, 60 kDa, 75 kDa (red), 100 kDa, 140 kDa, and 180 kDa. The Prestained Protein Marker 10-245 kDa is delivered in convenient 2 x 250µL aliquots. With a recommended application load of 3-5μL per lane, the marker lasts for up to 100 gels.

(Nitrotetrazolium Blue chloride, Ditetrazolium dye, Nitro BT). A ditetrazolium compound for demonstrating succinic dehydrogenase activity or glucose-6-phophate dehydrogenase or xanthine oxidase. Used in conjunction with BCIP for detection of alkaline phosphatase. (Negi D.S. and Stephens R.J. (1977) J. Histochem. Cytochem., 25, 149, Vila V. et al. (1984) Clin. Chim. Acta, 138, 215.

Galactosamine is a hexosamine derived from galactose with the molecular formula C6H13NO5. This amino sugar is a constituent of some glycoprotein hormones such as follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Other sugar constituents of FSH and LH include glucosamine, galactose and glucose.

Medicago’s pNPP substrate is specifically developed for immunoassay procedures and it is ideal for phosphate-based ELISA methods. The substrate is also used as a chromogenic non-specific substrate in alkaline and acid phosphatase assays. Its soluble end-product is yellow and can be spectrophotometrically read at 405 to 410 nm. The reaction may be stopped with 3 M NaOH. The pNPP substrate is supplied as pre-weighed tablets in bottles or in convenient blister packs, each tablet containing 5 mg or 20 mg of substrate. Directions for use:Deposit one tablet of the pNPP substrate in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized water in the appropriate amount to reach the required concentration for the assay. Stir until full dissolution and the substrate solution is ready to use.

CHES shows a pKa (25°C) of 9,55 which makes CHES useful as a buffering component in the pH range between 8.6 to 12.0. CHES interferes in the protein quantification according to Lowry. It is used in crystallisation of Phosphotriesterase (50mM) or in the chemical modification of Bacteriorhodopsin (10mM). Synonyms are: 2-(Cyclohexylamino)ethanesulfonic acid; 2-(N-Cyclohexylamino)ethanesulfonic acid. Other buffers are: BES, BICIN, MOPS, CAPS, CHAPS

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.05M Tris buffered saline, 0.138M NaCl, 0.0027M KCl, pH8.0 at 25°C.

Purity: >99% (HPLC). CAS: [10593-29-0] - C6H11NaO5S x 2 H2O

highly purified.

highly purified.

Contents of 1 pouch dissolved in deionized water and made up to 1000mL/5000mL yields: 0.02M Sodium phosphate buffer, pH7.0 at 25°C. Product description: Genaxxon's Sodium Phosphate buffer is provided in different formulations in order to suit most biochemical and molecular biology applications. Choose a buffer with a pH and molarity that fits your laboratory procedures. The NaPi buffer is supplied as exactly pre-weighed powder in sealed pouches giving two volumes, 1000mL and 5000mL. Dissolving the contents of one pouch in deionized water yields: 1) NaPi buffer 0.1M or 1M with pH 6.5 at 25°C. 2) NaPi buffer 0.1M or 0.02M with pH 7.0 at 25°C. 3) NaPi buffer 1M with pH 7.2 at 25°C. 4) NaPi buffer 0.1M or 1M with pH 7.4 at 25°C. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >). PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

Contents of 1 pouch dissolved in deionized water and made up to 1000mL/5000mL yields: 0.025M Tris, 0.192M glycine, pH8.3 at 25°C.

Content of 1 pouch dissolved in deionized water and made up to 1000mL yields a 3M Sodium chloride solution. No weighing necessary.

highly purified.

3-[(3-Cholamidopropyl)-dimethylammonio]-2-hydroxy-1-propane sulfonate

Trehalose, also known as mycose or tremalose, is a natural alpha-linked disaccharide formed by an alpha,alpha(1,1) glucoside bond between two alpha-glucose units. It can be synthesised by bacteria, fungi, plants, and invertebrate animals. It is implicated in anhydrobiosis the ability of plants and animals to withstand prolonged periods of desiccation. It has high water retention capabilities, and is used in food and cosmetics. The sugar is thought to form a gel phase as cells dehydrate, which prevents disruption of internal cellorganelles, by effectively splinting them in position. Rehydration then allows normal cellular activity to be resumed without the major, lethal damage that would normally follow a dehydration/rehydration cycle. Trehalose itself is not an antioxidant because it is a non-reducing sugar and does not contain nucleophilic groups in its molecule. However, it was reported to have antioxidant effects. Trehalose is a nonreducing sugar formed from two glucose units joined by a 1-1 alpha bond, giving it the name of alpha-D-glucopyranosyl-(1 - 1)-alpha-D-glucopyranoside. The bonding makes trehalose very resistant to acid hydrolysis, and therefore is stable in solution at high temperatures, even under acidic conditions. The bonding also keeps nonreducing sugars in closed-ring form, such that the aldehyde or ketone end groups do not bind to the lysine or arginine residues of proteins (a process called glycation). Trehalose is less soluble than sucrose, except at high temperatures >80°C. Trehalose forms a rhomboid crystal as the dihydrate, and has 90% of the calorific content of sucrose in that form. Anhydrous forms of trehalose readily regain moisture to form the dihydrate. Anhydrous forms of trehalose can show interesting physical properties when heat-treated. Trehalose aqueous solutions show a concentration dependent clustering tendency. Owing to their ability to form hydrogen bonds between one another, they self-associate in water to form clusters of various sizes. All-atom molecular dynamics simulations have shown that when reaching a concentration of 1.5-2.2 molar, the trehalose molecular clusters percolate and form large, continuous aggregates within the system. Source: Wikipedia (https://en.wikipedia.org/wiki/Trehalose)

Among biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns. This PBS buffer is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches. How to prepare buffer solution: Empty one pouch of NaPi buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 200mL to 300mL of deionized water for a 1000mL pouch and stir the solution for a few minutes. Adjust the volume up to 1000mL, stir and the buffer solution is ready to use.Other phosphate-based ready-to-use buffers::PBS without Potassium (0.01M) (D2033 >). PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

n-Decyl-b-maltoside (DM) >99.0% highly purified

L-(−)-Glucose is an enantiomer of the more common D-glucose. L-Glucose cannot be used by cells as an energy source because it cannot be phosphorylated by hexokinase.

4-Chloro-1-naphtol is peroxidase substrate suitable for use in immunoblotting. The endproduct of the enzymatic reaction is an insoluble blue precipitate that can be observed visually. The precipitate can is not removed by washing with water.

n-Nonyl b-maltoside (NM); Assay: >99.5% for crystallography.

highly purified.

Synonym: Adenosin, Adenosine, 9-β-D-Ribofuranosyladenine, Adenine riboside, Adenine-9-β-D-ribofuranoside, D-Adenosine

X-Gal (5-Brom-4-chlor-3-indolyl-β-D-galactopyranosid) is a chromogenic substrate for β-galactosidase. It produces a blue color that can easily be detected visually. X-Gal is used in the identification of lacZ + bacteria, especially for the detection of ß-galactosidase, which is expressed by the recombinant vectors. X-Gal is often used in conjunction with IPTG > (for the induction of the lac promoter (lac operon)), for the screening of blue colonies. It is also used for the screening of ß-galactosidase reporter gene activity in transfections of eukaryotic cells. X-Gal is a substrate of ß-galactosidase, which releases by cleavage of the substrate 5-bromo-4-chloro-3-indoxyl (X). The enzymatically cleaved indoxyl is oxidized to the insoluble indigo. As a stock solution, for example, a 20mg/mL X-Gal solution in dimethylformamide can be prepared. For the use in LB / Amp - plates this stock solution is to be diluted 1 : 1000 (final concentration 20μg/mL) or 1: 500 (final concentration 40μg/mL). The heated medium should thereby be cooled to about 50°C. The amount of X-Gal may be reduced if an X-Gal solution in DMF is sprayed to the agar plates (already with bacterial colonies!). X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Buffering substance in the pH range 6,1-7,5. Min. 99.5% pure. Good N.E. et al. (1966) Biochemistry 5, 467-77, Shipman jr. Ch. (1969) Proc. Soc. Exp. Biol. Med. 130, 305-28.

n-Nonyl b-maltoside (NM); Assay: >99% highly purified.

Rhamnose is a naturally occurring 6-deoxy monosaccharide consisting of six carbon atoms (hexose) and stereochemically derived from mannose (6-deoxymannose). Unlike the other naturally occurring sugars, this one exists only in the L-form as a building block of pectin chains. α-L-rhamnopyranose, the cyclic pyranose form of L-rhamnose. The name is derived from the occurrence of L-rhamnose in buckthorn plants, whose botanical name is Rhamnaceae.

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry, isoelectric focusing and two-dimensional electrophoresis. Useful for solubilizing membrane proteins and breaking protein-protein interactions. Its small micellar molecular weight (6150) and high critical micellar concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilisation for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Commonly used concentrations for IEF are 2-4% (v/v).

1 tablet dissolved in 100mL of deionized water yields: 0.9% sodium chloride. These tablets are prepared from pharmaceutical grade reagents. Sodium chloride is widely used in many laboratory routines. For greater convenience a blister pack with 10 tablets is also avaliable. The reagent is 99.99% pure.

Buffering substance. (Good N.E., Izawa S. (1972) Methods Enzymol. 24, 53-68, Sankar M., Bates R.G. (1978) Anal. Chem. 50, 1922-24). Does not interfere with Folin protein assay. MES partially decomposes when autoclaved in the presence of glucose.

Mixture of 6 protease inhibitors with a broad range activity against aspartate-, cysteine-, serine-, and metallo proteases as well as aminopeptidases. It is especially formulated for use with extracts from mammalian tissue, but can also be used with other extracts. This ready-to-use mix is free of EDTA and contains AEBSF >, Aprotinin >, E-64 >, Leupeptin >, Bestatine >, and Pepstatin A >. The kit is supplied with an extra vial of 1mL ultrapure DMSO. The content of 1 vial dissolved in 1mL DMSO will give a 100-fold concentrate, suitable for the treatment of 100mL extract. Activity spectrumAEBSF is an inhibitor against Serine proteases. Aprotinin is an inhibitor against Serine proteases. E-64 is an inhibitor against Cysteine proteases. Leupeptin is an inhibitor against Cystein proteases and trypsin-like Serine proteases. Peptstatin A is an inhibitor against Aspartic proteases. Bestatin is an inhibitor against Aminopeptidase B and Leucine-Aminopeptidases. More ready-to-use protease inhibitor mixes can be found here: - Protease-Inhibitor-Mix I > (general applications, contains no EDTA) - Protease-Inhibitor-Mix II > (general applications, contains EDTA)- Protease-Inhibitor-Mix III > (formulated for use with bacterial extracts, contains EDTA)- Protease-Inhibitor-Mix IV > formulated for use with plant extracts, contains no EDTA)- Protease-Inhibitor-Mix V > (formulated for use with mammalian extracts, contains no EDTA) You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

1 tablet dissolved in 100mL of deionized water yields: 0.05M Sodium Carbonate-bicarbonate buffer, pH9.6 at 25°C. No time consuming and expenisve calibration / pH-measuring necessary. Just dissolve buffer talets in water and use buffer solution. You will find more ready-to-use buffers here: ready-to-use buffers >

Protease-Inhibitor-Mix III for bacterial extracts is mixture of 5 protease inhibitors with a broad range activity against aspartate-, cysteine-, serine-, and metallo proteases as well as aminopeptidases. It is especially formulated for use with bacterial extracts. This ready-to-use mix contains AEBSF >, E-64 >, Pepstatin A >, Bestatine > and EDTA. The kit is supplied with an extra vial of 1mL ultrapure DMSO. The content of the inhibitor mix dissolved in 1mL DMSO lasts for the treatment of 100mL bacterial extract / bacterial lysate. The content of 1 vial dissolved in 1mL DMSO will give a 100-fold concentrate, suitable for the treatment of 100mL bacterial extract / bacterial lysate. Activity spectrumAEBSF is an inhibitor against Serine proteases. E-64 is an inhibitor against Cysteine proteases. Peptstatin A is an inhibitor against Aspartic proteases. Bestatin is an inhibitor against Aminopeptidase B and Leucine-Aminopeptidases and EDTA inhibits Metallo proteases. More ready-to-use protease inhibitor mixes can be found here: - Protease-Inhibitor-Mix I > (general applications, contains no EDTA) - Protease-Inhibitor-Mix II > (general applications, contains EDTA)- Protease-Inhibitor-Mix III > (formulated for use with bacterial extracts, contains EDTA)- Protease-Inhibitor-Mix IV > formulated for use with plant extracts, contains no EDTA)- Protease-Inhibitor-Mix V > (formulated for use with mammalian extracts, contains no EDTA) You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

The Protease-Inhibitor-Mix IV for plant extracts is a mixture of 6 protease inhibitors with a broad range activity against aspartate-, cysteine-, serine-, and metallo proteases as well as aminopeptidases. It is especially formulated for use with plant extracts. This ready-to-use mix contains AEBSF >, E-64 >, Leupeptin >, Bestatin >, Pepstatin A > and 1,10-Phenanthroline. The kit is supplied with an extra vial of 1mL ultrapure DMSO. The content of 1 vial dissolved in 1mL DMSO will give a 100-fold concentrate, suitable for the treatment of 100mL extract. More ready-to-use protease inhibitor mixes can be found here: - Protease-Inhibitor-Mix I > (general applications, contains no EDTA) - Protease-Inhibitor-Mix II > (general applications, contains EDTA)- Protease-Inhibitor-Mix III > (formulated for use with bacterial extracts, contains EDTA)- Protease-Inhibitor-Mix IV > formulated for use with plant extracts, contains no EDTA)- Protease-Inhibitor-Mix V > (formulated for use with mammalian extracts, contains no EDTA) You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

L-Fucose CAS 2438-80-4 (6-Deoxy-L-galactose) may be used for different purposes. 1. In studies of fucoidan polysaccharide containing glycans.2. L-Fucose is used as a substrate to identify, differentiate and characterize enzymes such as the fucosidase(s), l-fucose isomerase(s) and L-fucose dehydrogenase(s).3. L-Fucose may be used to study organelles, bacterial microcompartments, involved in the degradation of plant and algal cell wall sugars.4. L-Fucose may be used as a reference compound in rare sugar identification and analysis.5. A more and more important role of fucose,  is within the biopharmaceutical industry and, specifically, the production of monoclonal antibodies (mAbs). Antibody-dependent cell mediated cytotoxicity (ADCC) is the primary mechanism of action for many therapeutic monoclonal antibodies. The cytotoxic effect of the mAbs is more or less based on the portion of fucose of the N-linked glycans. The exact amount of fucose must be detected quickly and unambiguously while production. Pure fucose can serve as a standard for this purpose.

D-Luciferin also named Firefly Luciferin is the most popular and versatile bioluminescent substrate. Firefly luciferase produces light by the ATP-dependent oxidation (Mg2+ as cofactor) of luciferin. It emits a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase activity when luciferin and ATP are present in excess. Firefly luciferase has long been conjugated to antibodies and used as a label in immunoassays using luciferin as the substrate for detection. One particular advantage to the enzyme is that there is low endogenous luciferase activity in mammalian tissues besides its high sensitivity. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain. Luciferin is a common reagent used throughout the biotechnology field and specifically for in vivo imaging. Luciferase labeled tumor cells, stem cells or infectious diseases are often inoculated into research animals such as rats or mice for investigation. The injection of luciferin allows for the real-time, non-invasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI). Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays (e.g., determine cell viability and bacteria counting).

TBS Tween® 20 (Tris buffered saline with Tween) is one of the most commonly used biological buffers for plate based immunoassays and Western blotting. It is isotonic, non-toxic to cells and contains a non-ionic detergent. It is used preferably as an antibody diluent and wash reagent in ELISA and Western blotting using alkaline phophatase or peroxidase-conjugated antibodies. TBS-T buffer is supplied in exactly pre-weighed tablets, each tablet giving 500mL of 0.15M NaCl, 0.05% Tween® 20, 0.050M Tris-HCl buffer solution with pH7.6 at 25°C. Directions for use: Deposit one tablet in a laboratory flask or beaker placed on a magnetic stirrer. Add 200mL of deionized water and stir the solution for a few minutes. Adjust the volume up to 500mL, stir until full dissolution and the buffer is ready to use. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >). PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

Glycogen is used to precipitate nanograms of DNA very efficiently. Mainly used if nucleic acids are precipitated with 0.5M ammonia-acetate and iso-Propanol. Ref.: Sambrook J., & Russel D.W. (2001) Molecular Cloning: A Laboratory Manual, 3rd Edition, p. 5.20, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

This PBS tablets are specifically developed for immunological and microbiological laboratories. The buffer is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches. 1 tablet dissolved in 1000mL of deionized water yields: 0.01M Phosphate buffer, 0.14M NaCl, pH7.4 at 25° C.Other phosphate-based ready-to-use buffers:PBS without Potassium (0.01M) (D2033 >). PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

n-Dodecyl-ß-maltoside (DDM) >99.5% especially suitable for use in crystallography. n-Dodecyl-β-D-maltoside (DDM), is a water-soluble non-ionic detergent, for isolation and stabilization of hydrophobic membrane proteins. n-Dodecyl-β-D-maltoside is a gentle detergent that preserves protein activity better than many other detergents, such as CHAPS or octyl-β-glucoside. The protein-sparing properties of this high-purity DDM allow it to be used, for example, in nasal sprays, as the DDM leads to improved mucus clearance.

1 tablet dissolved in 100mL of deionized water yields: 0.01M Phosphate buffer, 0.0027M KCl, 0.14M NaCl, pH7.4 at 25°C. Amoung biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns. Our PBS is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches. There are different standard-sized packages and final volumes range from 100mL to 100 litres. Choose from different pH (7.2 or 7.4), from PBS with our without potassium, or with or without Tween. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >). PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

Bicine is recommended for low temperature biochemical work and for the preparation of stable substrate solution for serum guanase determination. Bicine is used for different applications, ranging from buffers used in enzymatic reactions to electrophoretic buffers. Bicine can not be used for BCA and Lowry protein determination procedures. Working concentrations: 3 mM up to 100 mM.

24 respective 100 ready-to-use p-Nitrophenylphosphate (pNPP) tablets with 20mg p-Nitrophenylphosphate. Each tablet dissolved in 20mL deionized water yields a solution containing 1.0mg/mL pNPP, 0.2M Tris buffer. Genaxxon's pNPP substrate is specifically developed for immunoassay procedures and it is ideal for phosphate-based ELISA methods. The substrate is also used as a chromogenic non-specific substrate in alkaline and acid phosphatase assays. Its soluble end-product is yellow and can be spectrophotometrically read at 405 to 410 nm. The reaction may be stopped with 3M NaOH. The pNPP substrate is supplied as pre-weighed tablets in bottles or in convenient blister packs, each tablet containing 5mg or 20mg of substrate. How to use:Deposit one tablet of the pNPP substrate in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized water in the appropriate amount to reach the required concentration for the assay. Stir until full dissolution and the substrate solution is ready to use. One tablet dissolved in 20mL of substrate buffer results in a 1mg/mL solution.