Molecular Biology

Agarose LE is a standard agarose for the separation of DNA in the size range between 100bp and 25kbp. It is suitable for all analytical and preparative electrophoresis of nucleic acids in routine gel electrophoresis. Depending on the concentration of Agarose LE used, the size range of nucleic acid separation will vary between 100bp and 25kbp. The low EEO makes this useful for a broad range of applications: PCR product analysis, restriction enzyme digest analysis, separation of RNA before blotting, etc. This agarose is comparable with, e.g. Agarose BioRagent, low EEO from Sigma or with the Universal-Agarose, peqGOLD from Peqlab. Free Taq DNA Polymerase test sample available! No shipping costs within Germany. Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available. Standard normal melting agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 100bp up to 25kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. They are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, agarase, as well as for "in-gel" DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.

Agarose LE tablets of 0.5g each for separation of DNA in the size range between 100bp and 25kbp. Agarose LE is suitable for all analytical and preparative electrophoresis of nucleic acids in routine gel electrophoresis. Depending on the concentration of Agarose LE used, the size range of nucleic acid separation will vary between 100bp and 25kbp. The low EEO makes this useful for a broad range of applications: PCR product analysis, restriction enzyme digest analysis, separation of RNA before blotting, etc.. Very easy to handle: No weighing No dust Easy to dissolve Comparable to SeaKem® LE. Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available.Standard high melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 50kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. They are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, agarase, as well as for "in-gel" DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.

Agarose LM is the original low melting agarose. This "molecular biology grade" agarose gives gels with better properties and higher transparency than the standard normal melting point agarose. The agarose is equivalent to SeaPlaque™ from Lonza. Agarose LM is a low melting agarose with a very high separation capacity for large DNA fragments (>1000 bp), RNA and proteins. Enzymatic manipulations (eg, digestion, ligation, PCR, etc.) can be performed in the molten gel. Extraction of the DNA is therefore not necessary. Low melting Agarose LM is excellently suited for digestion with β-agarase (S5223). Large DNA fragments can be isolated very gently. The low melting agarose LM is used in the analytical and preparative field. For optimal results, the gel should be stored for at least 1 hour before use at +2°C to +8°C. Genaxxon offers agaroses for a wide variety of applications. There are agaroses with different melting points or with normal or high-resolution separation properties of DNA fragments. Standard melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (Standard Agarose LE (M3044 >)) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.

Agarose Mega resolved DNA and RNA fragments from >100bp up to 50kb. Due to the high gel strength the agarose is ideally suited for Southern- and Northern blotting experiments or Pulsed Field Gel Electrophoresis (PFGE) as gel will not break during handling or electrophoresis. Due to the high gel strength, low concentrations of agarose may be used and high molecular weight molecules migrate rapidly without significant resctriction. The high gel strength reduces separation times for large DNA molecules while maintaining high resolution. Agarose Mega can also be used for the preparative separation of nucleic acids or for Ouchterlony or radial immundiffusion experiments. Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available. Standard agarose high melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.

Agarose Tiny is a low-melting high resolution agarose that is suitable for molecular biology applications and produces gels with better separation properties and greater clarity than standard agarose. It is very transparent even at high gel concentrations (3.0 - 4.5%) and can be used with all buffer systems. The low melting temperature makes Agarose Tiny ideal for preparative DNA and RNA electrophoresis, while the low gelling temperature is ideal for tissue culture cell cloning experiments and viral plaque assays. In addition to the above mentioned properties, Agarose Tiny can also be used for high-resolution (2 bp) gel electrophoresis in the range from 10 to 500 bp. Example: PCR products or small DNA fragments after restriction digestion or in mutation analyses. Features:Like Lonza's SeaPlaque™, Agarose Tiny has a low melting point (<64°C, 3.0% gel) and gel point (<25°C, 3.0% gel) and low DNA-binding capacity. Their use is particularly recommended for preparative RNA and DNA gels. Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available. Standard agarose high melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.Recommended for the following applications:- RFLP (Restriction Fragment Length Polymorphism - AMPFLP (Amplified Fragment Length Polymorphism) - HVR (Hyper-Variable Regionen) - VNTR (Variable Numbers of Tandem Repeats) - STR (Short Tandem Repeats) - Tri und Tetra Nukleotid repeats

Our Agarose Tiny HT is a high resolution (+/-2 bp), normal melting agarose for fine resolution of small DNA fragments in the 20 bp to 1500 bp range. It can be used for genotyping, allele sizing and short tandem repeat analysis. Agarose Tiny works as a molecular screen and has twice the resolution of the finest sieving agaroses. It can discriminate between fragments which differ by only 2-4 bp in length and thus, is able to compete with acrylamide gels, being much easier to handle as the latter. Agarose Tiny HT 2% to 4% gels give similar results to Polyacrylamide 6% to 8% gels. For efficient resolution, the concentration of Agarose Tiny HT should be adjusted according to the range of band sizes analyzed, and the gel running buffer used (TAE or TBE). Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available.Standard agarose high melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.

Genaxxon’s DNA Library Prep Kit for Illumina is your universal solution for preparing high-quality libraries for sequencing on Illumina high-throughput platforms. Designed to convert fragmented DNA input ranging from 1 ng to 1 μg into sequencing-ready libraries, this kit offers unmatched efficiency and reliability for your sequencing projects.WorkflowKey Features:Wide Compatibility: Ideal for various sample types, including low-quality FFPE and cfDNA, as well as conventional DNA samples.High Efficiency: Delivers superior library conversion rates, ensuring maximum yield from your input DNA.Accurate Detection: Enables precise identification of low-frequency mutations, making it perfect for sensitive sequencing applications.Streamlined Workflow: Experience a seamless, four-step workflow that completes in approximately three hours—all within a single reaction tube. This process eliminates the need for intermediate purification and sample transfers, reducing the risk of handling errors and preserving the integrity of your samples. With DNA Repair Enzymes II, the kit effectively repairs a wide range of DNA damages, including nicks, gaps, and oxidized bases, ensuring the highest quality libraries for your sequencing needs. Applications: Whole-genome sequencing Whole-exome or targeted capture sequencing Amplicon sequencing ChIP sequencing Metagenome sequencing Methylation sequencing Optimized for IlluminaThe resulting libraries are fully compatible with standard Illumina® sequencing reagents and protocols, delivering exceptional performance for high-coverage deep sequencing, de novo sequencing, and whole-genome resequencing projects.Compatibility: This kit is fully compatible with Genaxxon’s Unique Dual Index primer pairs. For 24 reactions, use the UDI MidiSet (M4402). For 96 reactions, you can either use 2x UDI Set A (M4403) or 1x UDI Set A (M4403) and 1x UDI Set B (M4404).

Genaxxon’s DNA Library Prep Kit PLUS for Illumina is an advanced solution designed to enhance your sequencing workflow on Illumina high-throughput platforms. This kit simplifies DNA library preparation by integrating DNA fragmentation, end repair, and dA-tailing into a single step. With adapter ligation, library amplification, and size selection performed directly—without additional purification—the entire process is completed in just 2 hours.WorkflowKey Features:Efficient Workflow: Streamline your process by performing fragmentation, end repair, and dA-tailing with a single enzyme mix, finishing in under 2 hours.Versatile Insert Sizes: Customize your insert sizes by adjusting fragmentation time, while minimizing inversions and chimeras for optimal results.Broad Compatibility: Designed for diverse DNA sources, with strict background bacterial control for accurate pathogen detection.Optimized Performance: The Genaxxon DNA Library Prep Kit PLUS is compatible with DNA from various sources, including challenging samples like FFPE DNA. Enhanced reagent optimization reduces false positives, improving sequencing yield and overall results. This kit is particularly well-suited for constructing PCR-free libraries using 100 ng of high-quality genomic DNA. Applications: Whole-genome sequencing Exon or other targeted sequencing Metagenomic sequencing Optimized for IlluminaGenaxxon’s DNA Library Prep Kit PLUS delivers exceptional performance for a range of sequencing applications, ensuring your libraries are fully compatible with Illumina® sequencing reagents and protocols, making it the ideal choice for efficient, high-quality sequencing preparation.Compatibility: This kit is fully compatible with Genaxxon’s Unique Dual Index primer pairs. For 24 reactions, use the UDI MidiSet (M4402). For 96 reactions, you can either use 2x UDI Set A (M4403) or 1x UDI Set A (M4403) and 1x UDI Set B (M4404).

DNase I is an endonuclease isolated from bovine pancreas that digests double- and single-stranded DNA into oligo- and mono-nucleotides. DescriptionDNAse I is used, among other things, to avoid unwanted cell clumping during tissue disaggregation, which makes dispersion of individual cells difficult. Since tissue disaggregation and subsequent isolation of single cells is always accompanied by rupture and lysis of some cells, DNA is released from these cells into the surrounding medium. The released DNA in the medium is thought to be responsible for undesirable cell clumping during tissue dissociation procedures. This clumping effect can be avoided by adding deoxyribonuclease (DNase) to the dissociation medium and has been described for a variety of different cell and tissue types. DNase I is suitable for eliminating DNA from RNA preparations prior to sensitive applications, such as RT-PCR. Since no RNA purification procedure removes 100% of the DNA, RNA samples should be digested with DNase I before RT-PCR. A simple 15 minute digestion at room temperature removes the contaminating DNA. DNase I is inactivated by adding the stop solution and heating. Heating also denatures the RNA, so the RNA can be used directly for reverse transcription. This DNAse I is not RNase-free and has to be chemically treated if used for RNA isolation procedures.

The GenLadder 50bp (ready-to-use) is an ideal DNA size marker for fragment sizes from 50 - 1500bp. The bands at 200bp, 500bp and 1200bp bands light up more intensive for better orientation. Each band has been calibrated with respect to base pair size. Thus, an accurate determination of product size is possible. No ambiguous bands are visible. Band sizes: 50 (ca. 30ng/5µL), 100 (ca. 27ng/5µL), 150 (ca. 30ng/5µL), 200 (2x) (ca. 67ng/5µL), 250 (ca. 25ng/5µL), 300 (ca. 20ng/5µL), 350 (ca. 18ng/5µL), 400 (ca. 27ng/5µL), 450 (ca. 23ng/5µL), 500 (2x) (ca. 67ng/5µL), 600 (ca. 20ng/5µL), 700 (ca. 23ng/5µL), 800 (ca. 27ng/5µL), 900 (ca. 30ng/5µL), 1000 (ca. 33ng/5µL), 1200 (2X) (ca. 70ng/5µL) and 1500bp (ca. 40ng/5µL). GenLadder 50bp is already dissolved in loading buffer with tracking dye (Orange G) that does not overlab with bands in the range between 500bp and and smaller. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

Our DNA marker GenLadder 100 bp Plus with loading dye is an ideal tool to determine the size of double stranded DNA in the range of 100 - 3000 bp. The additional bands at 1.5kbp and 3.0kbp allow to assign additional DNA fragment sizes in the range up to 3000 bp without using an additional 1kbp DNA marker. The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp. The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 3000 bp (40ng/5µL), 2x 1500 bp (70ng/5µL), 1000 bp (50ng/5µL), 900 bp (40ng/5µL), 800 bp (40ng/5µL), 700 bp (30ng/5µL), 600 bp (30ng/5µL), 2x 500 bp (90ng/5µL), 400 bp (40ng/5µL), 300 bp (30ng/5µL), 200 bp (40ng/5µL), 100 bp (40ng/5µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 0.1µg/µL (108µg/mL). The recommended amount of DNA marker per lane is 0,5μg (5µL). GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

The GenLadder 100 bp Plus with gel staining dye and loading dye - ready-to-use is an innovation for high throughput approaches or colony screenings! Ideally suited to determine the size of double-stranded DNA between 100 and 1000 base pairs. The additional bands at 1.5kbp and 3.0kbp allow DNA sizes in this range to be assigned without having to use an additional 1kbp DNA marker. At the same time, the mixture also contains a fluorescent gel staining dye detectable with blue light (ca. 490nm excitation). Additional staining (pre- or post staining) or the addition of fluorescent dye prior to application is thus unnecessary and saves an additional pipetting step. This DNA ladder is an ideal match for our Red MasterMix Fluoro 2X, which also contains the gel staining dye. Together the ideal pair for Colony Screens! The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp. The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 3000 bp (40ng/6µL), 2x 1500 bp (70ng/6µL), 1000 bp (50ng/6µL), 900 bp (40ng/6µL), 800 bp (40ng/6µL), 700 bp (30ng/6µL), 600 bp (30ng/6µL), 2x 500 bp (90ng/6µL), 400 bp (40ng/6µL), 300 bp (30ng/6µL), 200 bp (40ng/6µL), 100 bp (40ng/6µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 90 µg/mL. The recommended amount of DNA marker per lane is 0,5μg (6µL).Please note: The included dye does show a small excitation peak in the UV range, but fluorescence is significantly stronger under blue light excitation. We therefore recommend using blue light to achieve the best possible results. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3084 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

The GenLadder 1kb (ready-to-use) 250bp - 10kbp-ladder is the ideal DNA size marker for fragment sizes from 250 - 10000bp. All bands light up to the same intensity except the 1000 bp and the 3000 bp band which are twice as bright for better orientation. Each band has been calibrated with respect to base pair size and DNA quantity. Thus, an accurate determination of product size and DNA concentration is possible. No ambiguous bands are visible. The GenLadder 250bp - 10kb can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. The DNA is already dissolved in buffer with 6X loading dye ready to use. Fragment sizes and amount of DNA per band: 10000bp ( 28ng/5µL), 8000bp (28ng/5µL), 6000bp (28ng/5µL), 5000bp (28ng/5µL), 4000bp ( 18ng/5µL), 2x3000bp (92ng/5µL), 2500bp (34ng/5µL), 2000bp (34ng/5µL), 1500bp (20ng/5µL), 2x1000bp (92ng/5µL), 750bp (23ng/5µL), 500bp (30ng/5µL), 250bp (45ng/5µL). GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

With the synthetic GenLadder XLarge, even larger plasmid insertions and vector plasmids can be determined clearly and precisely in size by gel electrophoresis in the range of 250 - 25000 base pairs after restriction digests. The Genaxxon GenLadder XLarge shows 14 bands. The approximate mass of the DNA in each band is given for a 5µL load (0.52µg/lane). Additionally the 1000 bp and 3000 bp are intensified for better and faster orientation in 1.0 - 1.5% agarose gels. Please avoid repeated freez-thaw cycles. We recommend to portion the product into smaller aliquots. Source: PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH8.0) and 10mM EDTA. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

Restriction enzyme-digested lambda DNA (48,502bp) generates molecular weight size markers routinely used in gel analysis of nucleic acids. The complete nucleotide sequence has been determined. Lit.: Sanger, F. et al. (1982) J. Mol. Biol. 162, 729. Details about the DNA sequence can be found in the data sheet attachement. Description/Preparation: The double stranded DNA is isolated from bacteriophage lambda (cl857 ind1 Sam7). The molecular weight is 31.5 x 10E6 daltons and is 48,502 base pairs in length.The phage is isolated from the heat inducible lysogen E. coli λ cl857 S7 by gel filtration. The DNA is isolated from the purified phage by phenol/chloroform extraction and dialyzed against 10mM Tris-HCl (pH7.4) and 1mM EDTA. The product is methylated. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye which contains bromophenol blue and xylene cyanol FF): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

NGS DNA Puri MagBeads is a SPRI-based chemistry that speeds and simplifies nucleic acid size selection and PCR purification for DNA and/or RNA library preparation for Next Generation sequencing. It is compatible with mainstream NGS library prepared kits. NGS DNA Puri MagBeads utilize SPRI (Solid Phase Reverse Immobilization) paramagnetic bead technology to enable DNA purification and size selection during NGS library construction.NGS DNA Puri MagBeads are compatible with most NGS library construction kits (DNA, RNA). The kit allows users to seamlessly replace competitor beads (i.e. AMPure XP Beads) in current bead clean-up and size selection protocols without loss of efficiency. Why Purification and Quality Control of DNA preps for NGS?The purification step is usually necessary to remove unwanted material that might interfere with sequencing. Some NGS platforms may have tight size requirements, and discarding fragments that are too large or too small can improve sequencing efficiency by far. The optimal library size is given by the sequencing application. It is common to clean up nucleic acids using magnetic beads. Quality control is the last process before sequencing. As Subsequent experiments are time-consuming and expensive, strict quality control steps are required to ensure all samples are appropriate for their applications. Confirming the quality and quantity of DNA increases the reliability of sequencing data! The Genaxxon NGS MagBead DNA purification kit protocol can be adapted to your current liquid handling workstation (e.g., Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.

Overview:Proteinase K is a robust and versatile serine protease used extensively in molecular biology for the digestion of proteins in nucleic acid preparations. This solution, at a concentration of 20 mg/mL, is ideal for a wide range of applications including the preparation of DNA and RNA samples. The PCR grade quality ensures that the enzyme is free from contaminants that could interfere with downstream applications, making it highly suitable for sensitive techniques such as PCR.Product Details:ready-to-useConcentration: 20 mg/mLactive over a wide range of reaction conditionsAn elevation of the reaction temperature from 37°C to 50 - 60°C may increase the activity several timesQuality: PCR grade, ensuring high purity and no interference with PCR reactions.Applications: Suitable for DNA and RNA extractionRemoval of protein contaminantsPreparation of samples for various molecular biology techniques.Benefits:High Purity: The PCR grade quality guarantees minimal contamination, ensuring reliable results in sensitive applications. Versatility: Ideal for various molecular biology procedures, including sample preparation for PCR.Convenience: Ready-to-use solution at an optimal concentration for ease of use. Notes:The recommended working concentration of Proteinase K is 0.05 to 1 mg/mL. The activity of the enzyme is stimulated by 0.2 to 1% SDS and also by 1 to 4M ureaCa2+ protects Proteinase K against autolysis and increases the thermal stabilityStable over a wide pH range: 4.0 to 12.5, optimum pH 7.5 to 8.0Activity optimum: 50°C to 55°CRapid denaturation of enzyme occurs at temperatures above 65°CFeatures:No detected exonuclease, endonuclease or RNase activitySpecific activity: >40 units/mg protein Activity: ≥30 units/mg lyophilizateSolubility: ≥20 mg/mLDNA content ≤10 pg/mgShipping conditions: Shipment on wet iceInhibitors of proteinase KWhich substances can inhibit proteinase K? Proteinase K can be inhibited by a variety of substances. These include ‘denaturing substances’, detergents and proteinase inhibitors. Sensitivity to inhibitors depends pretty much on the specific application (exact pH, concentration, temperature). Inhibitors frequently used in biochemistry and molecular biology are:SDS: High concentrations of SDS can denature and inactivate proteinase K.EDTA: EDTA is a chelating agent that can bind to metal ions that are essential for proteinase K activity.Urea: High concentrations of urea can denature and thereby inactivate proteinase K.Detergents: Some detergents, such as Triton X-100 or Tween® 20, can inhibit proteinase K, especially when the detergents are present in high concentrations.Protease inhibitors: Protease inhibitors, such as phenylmethylsulfonyl fluoride (PMSF), can irreversibly inhibit proteinase K.Chaotropic salts: Depending on the DNA extraction protocol, the presence of chaotropic salts or detergents may increase proteinase K activity. However, the same reagents may also inhibit proteinase K activity.Proteinase K can be obtained from Genaxxon as Powder (M3036) > or as 20mg/mL Solution (M3037) >.Produced by Genaxxon bioscience GmbH, founded in 2002 by Dr. Norbert Tröndle to provide reliable products for PCR and custom cell culture media formulations, the Proteinase K Solution 20 mg/mL offers a dependable and efficient solution for your molecular biology needs. Achieve consistent, high-quality results with an enzyme designed specifically for your requirements.

Overview:Proteinase K is a robust and versatile serine protease used extensively in molecular biology for the digestion of proteins in nucleic acid preparations. This solution, at a concentration of 20 mg/mL, is ideal for a wide range of applications including the preparation of DNA and RNA samples. The PCR grade quality ensures that the enzyme is free from contaminants that could interfere with downstream applications, making it highly suitable for sensitive techniques such as PCR.Product Details:ready-to-useConcentration: 20 mg/mLactive over a wide range of reaction conditionsAn elevation of the reaction temperature from 37°C to 50 - 60°C may increase the activity several timesQuality: PCR grade, ensuring high purity and no interference with PCR reactions.Applications: Suitable for DNA and RNA extractionRemoval of protein contaminantsPreparation of samples for various molecular biology techniques.Benefits:High Purity: The PCR grade quality guarantees minimal contamination, ensuring reliable results in sensitive applications. Versatility: Ideal for various molecular biology procedures, including sample preparation for PCR.Convenience: Ready-to-use solution at an optimal concentration for ease of use. Notes:The recommended working concentration of Proteinase K is 0.05 to 1 mg/mL. The activity of the enzyme is stimulated by 0.2 to 1% SDS and also by 1 to 4M ureaCa2+ protects Proteinase K against autolysis and increases the thermal stabilityStable over a wide pH range: 4.0 to 12.5, optimum pH 7.5 to 8.0Activity optimum: 50°C to 55°CRapid denaturation of enzyme occurs at temperatures above 65°CFeatures:No detected exonuclease, endonuclease or RNase activityConcentration ≥20 mg/mL / Activity ≥600 U/mLDNA content ≤200 pg/mLShipping conditions: Shipment on wet ice Storage buffer: 10 mM Tris/HCl, pH7.5; 1mM (CH3COO)2Ca; 50% glycerolProteinase K can be obtained from Genaxxon as Powder (M3036) > or as 20mg/mL Solution (M3037) >.Produced by Genaxxon bioscience GmbH, founded in 2002 by Dr. Norbert Tröndle to provide reliable products for PCR and custom cell culture media formulations, the Proteinase K Solution 20 mg/mL offers a dependable and efficient solution for your molecular biology needs. Achieve consistent, high-quality results with an enzyme designed specifically for your requirements.

Genaxxon Proteinase Low-Temp is a nonspecific endopeptidase derived from an Arctic marine microbial source. It has a broad substrate specificity and is easy to inactivate after use. Starting at a temperature of 60°C, the Genaxxon Proteinase Low-Temp is heat-inactivated, making it ideal for heat-sensitive samples. The Proteinase Low-Temp is most active in the temperature range of 25°C-40°C. Recommended inactivation conditions are: Typically up to 15 minutes at 40°C – 65°C (inactivation by denaturation). It is known that histones and other proteins shield nucleic acids from an optimal interaction with other DNA-binding proteins and enzymes. Genaxxon Proteinase Low-Temp is ideal for transforming chromatin and other compact nucleic acids into mere DNA. The enzyme is easily inactivated. This allows thermal inactivation at temperatures that provide RNA integrity and avoid the dissociation of dsDNA.Advantages:- Easy to deactivate- Active at high salt content- Compatible with downstream analyzes Application examples:Preparation of chromosomal DNA for PFGE- Degradation of nucleases (DNase, RNase) in the preparation of nucleic acids, including the recovery of native RNA- Isolation of genomic DNA from tissue of mouse tails- Isolation of genomic DNA from cell culturesSpecific activity: >30 U/mg. Foreign activity: RNase and DNase undetectable. Temperature optimum: + 25°C-40°C (pH 7-10). Recommended inactivation conditions: Typically up to 15 minutes at 40°C – 65°C (inactivation by denaturation).Inhibitors: General Serine protease inhibitors such as PMSF (M3194) >.Instructions for use: The enzyme is stable up to 2 years when stored at -20°C. Genaxxon Proteinase Low-Temp is stable even when stored at 4°C (>6 months) and room temperature (>4 weeks).The optimal enzyme activity is between pH 7 and 10. Recommended temperature range for the activity is 25-40°C. The activity of Genaxxon Proteinase Low-Temp is not dependent on divalent cations such as calcium (Ca2 +) and is therefore active in buffers containing EDTA (<40°C reaction temperature).Genaxxon Proteinase Low-Temp is suitable for the digestion of proteins in the presence of SDS (0.2-1%) and urea (1-5 M). The enzyme tolerates 500mM guanidine thiocyanate and 1% Triton X-100 (>50% activity). There is no significant loss of activity at 400 mM NaCl. Proteinase K is used for the digestion of proteins in cell lysates (tissue, cell culture cells) and for the release of nucleic acids, since it very effectively inactivates DNases and RNases. The digest with Proteinase K for the purification of nucleic acids is performed in the presence of EDTA (inhibition of magnesium-dependent enzymes). Proteinase K can be obtained from Genaxxon as Powder (M3036) > or as 20mg/mL Solution (M3037) >.

Ribonuclease A is an endo-ribonuclease specifically cleaving single stranded RNA at the 3' side of pyrimidine bases (cytosine and uracil). RNAse A ist used to prepare RNA-free DNA, to digest non-hybridised regions of RNA-DNA hybrids and as a molecular weight marker. The pH optimum for RNAse A is between 7.0-7.5. RNAse A is inhibited by Diethylpyrocarbonate (DEPC) >, guanidinium salt (4M GuaSCN), beta-Mercaptoethanol, heavy metals, vanadyl-ribonucleoside complexes, RNAse-inhibitor from human placenta and by competitive DNA. RNAse A cleaves single- and double-stranded RNA and RNA in RNA:DNA hybrids at low salt conditions (100 mM NaCl). At high salt conditions (>300 mM NaCl), RNAse A cleaves single-stranded RNA only. The enzyme can only be removed by Proteinase K digest and subsequent phenol extraction. Elimination of DNAse activity: Product S5231 (RNAse A (not certified DNAse free)) may contain DNAse activity. As RNAse A is heat stable it is recommended to heat inactivate DNAse activity before use. Procedure: 10mg/mL RNAse A in 0.01 sodium acetate (pH5.2) have to be heated to 100°C for 15 minutes in a water bath. After 15 minutes, the RNAse shall remain in the water bath while the water cools down to room temperature. pH can be adjusted by addition of 0.1 time the volume of 1M Tris-HCl (pH7.4). After aliquotation of the now DNAse free RNAse A solution, each aliquote should be stored a -20°C. NOTE: RNAse A precipitates if concentrated solutions are heated to 100°C at neutral pH! Stock solution: Stock solutions are prepared at concentrations from 1 - 10mg/mL in 10mM Tris/HCl, pH7.5; 15mM NaCl or in 10mM Tris/HCl, pH7.5; 1mM EDTA, pH8.0 (TE buffer).The recommended working concentration is 10µg/mL (removal of RNA from plasmid preparations; 1hr, RT) or 100ng/mL (preparation of 'blunt ends' of double-stranded cDNA). Stability:RNase A aggregates during lyophilizing and storage. It has a high affinity to glas surfaces, which has to be taken into consideration. At neutral pH (e. g. in PBS pH 7.4) and high concentrations (>10mg/mL) the enzyme will precipitate. At +2°C to +8°C (lyophilized) it is stable for several years (dry storage), in solution (-20°C) several years or (+2°C to +8°C) several weeks.

Ribonuclease A- DNase-free is a DNase and Protease-free enzyme that can be used directly for RNA digests, e.g. in DNA purification procedures. Elimination of potential DNAse activity is not necessary. Potential DNAse activities have been removed. DescriptionRibonuclease A is an endo-ribonuclease specifically cleaving single stranded RNA at the 3' side of pyrimidine bases (cytosine and uracil). RNAse A ist used to prepare RNA-free DNA, to digest non-hybridised regions of RNA-DNA hybrids and as a molecular weight marker. The pH optimum for RNAse A is between 7.0-7.5. RNAse A is inhibited by Diethylpyrocarbonate (DEPC) >, guanidinium salt (4M GuaSCN), beta-Mercaptoethanol, heavy metals, vanadyl-ribonucleoside complexes, RNAse-inhibitor from human placenta and by competitive DNA. RNAse A cleaves single and douple stranded RNA and RNA in RNA:DNA hybrids at low salt conditions (100mM NaCl). At high salt conditions (>300 mM NaCl), RNAse A cleaves single-stranded RNA only. The enzyme can only be removed by Proteinase K digest and subsequent phenol extraction. Stock solution: Stock solutions are prepared at concentrations from 1 - 10mg/mL in 10mM Tris/HCl, pH7.5; 15mM NaCl or in 10mM Tris/HCl, pH7.5; 1mM EDTA, pH8.0 (TE buffer).The recommended working concentration is 10µg/mL (removal of RNA from plasmid preparations; 1hr, RT) or 100ng/mL (preparation of 'blunt ends' of double-stranded cDNA). Stability:RNase A aggregates during lyophilizing and storage. It has a high affinity to glas surfaces, which has to be taken into consideration. At neutral pH (e. g. in PBS pH 7.4) and high concentrations (>10mg/mL) the enzyme will precipitate. At +2°C to +8°C (lyophilized) it is stable for several years (dry storage), in solution (-20°C) several years or (+2°C to +8°C) several weeks.

SafeGel green stain is an ultra sensitive, extremely stable fluorescent dye designed to replace the toxic and possibly mutagenic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. SafeGel green stain is far more sensitive than EtBr without requiring a destaining step. SafeGel green stain is far less toxic and mutagenic compared to EtBr while both show virtually the same UV-spectra, so you can directly replace EtBr with SafeGel green stain without changing your existing imaging system. SafeGel can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. SafeGel can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. SafeGel is also compatible with downstream DNA manipulations such as digestion with a restriction enzyme, Southern blotting techniques and clonings. A series of safety tests has confirmed that SafeGel is noncytotoxic, nonmutagenic and nonhazardous even at concentrations above the working concentrations used in gel staining. As a result, SafeGel can be safely disposed in regular trash, providing convenience and reducing cost in waste disposal. This fluorescent dye is supplied as a 10,000X solution in water. For customers who look for large pack size, we offer a cost-saving bulk pack size of 2mL, 5mL or 10mL (M3193.1010). For high-class agarose gels we offer the Genaxxon standard agarose LE > or our speciality agaroses for high resolution Tiny (M3046) > and Tiny HT (M3047) >.

SafeGel red stain is an ultra sensitive, extremely stable fluorescent dye designed to replace the toxic and possibly mutagenic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. SafeGel red stain is far more sensitive than EtBr without requiring a destaining step. SafeGel red stain is far less toxic and mutagenic compared to EtBr while both show virtually the same UV-spectra, so you can directly replace EtBr with SafeGel red stain without changing your existing imaging system. SafeGel can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. SafeGel can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. SafeGel is also compatible with downstream DNA manipulations such as digestion with a restriction enzyme, Southern blotting techniques and clonings. A series of safety tests has confirmed that SafeGel is noncytotoxic, nonmutagenic and nonhazardous even at concentrations above the working concentrations used in gel staining. As a result, SafeGel can be safely disposed in regular trash, providing convenience and reducing cost in waste disposal. This fluorescent dye is supplied as a 10,000X solution in water. For customers who look for large pack size, we offer a cost-saving bulk pack size of 2mL, 5mL or 10mL (M3193.1010). Read in our blog why you should use Genaxxon's SafeGel now. For high-class agarose gels we offer the Genaxxon standard agarose LE > or our speciality agaroses for high resolution Tiny (M3046) > and Tiny HT (M3047) >.

DNA extracted from salmon testes. To prevent unspecific binding of a DNA to the membrane in Southern- and Northern-Hybridisations salmon sperm DNA is added to the pre-hybridisation solution (Blotto, Denhardt's etc.). This Salmon Sperm DNA shows a low molecular weight. The lyophilised DNA can be dissolved in sterile bidistilled water (stock solution e. g. 10mg/mL) and stored at -20°C. This DNA is used with a final concentration of 100μg/mL (Ref.: Sambrook, J., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, NY (1989), 9.48-9.49, 13.15. Silhavy, T., et al., Experiments with Gene Fusions, Cold Spring Harbor, NY (1984), 195.)

Ready-to-use SSC buffer solution of pH7.0 +/-0.2. Widely used in molecular biology experiments for the preparation of prehybridisation and hybridisation solutions, wash solutions, etc..

(2,7-Diamino-10-ethyl-9-phenylphenanthridium bromide) Ethidium bromide is an intercalating agent for nucleic acids. It is widely used for staining of nucleic acids after electrophoresis on agarose or acrylamide gels and for fluorescent labeling on a cesium chloride gradient. As Ethidium bromide is a powerful mutagen and moderately toxic it is highly recommended to use gloves and the GENAXXON bioscience ready-to-use solutions. Reference: Lunn G. and Sansone E.B. (1987) Anal. Biochem., 162, 453 - Sambrock J., Fritsch & E.F. and Maniatis T (1989) Molecular Cloning, A Laboratory Manual, 2nd Edition, Cold Spring Harbor, New York.

T4 DNA Ligase catalyzes the formation of a phosphodiester bonds between 5' phosphate and 3' hydroxyl termini in duplex DNA/RNA. The enzyme can join blunt end and cohesive end termini, repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.Cohesive End Ligation:For most cohesive end ligations, a 30 minute incubation at 20°C is sufficient. Incubations at 16°C for 4-16 hours are routinely used for the majority of applications. Ligation of blunt ends and single-base pair overhang fragments requires more enzyme to achieve the same extent of ligation as cohesive end DNA fragments. Ligation may be enhanced by addition of PEG, or by reducing the rATP concentration. - T4 DNA Ligase requires ATP > as a cofactor.- T4 DNA Ligase is strongly inhibited by NaCL or KCl if their concentration exceeds 200mM.- Ligation of blunt-ended and single-base pair overhang fragments requires about 50-times as much enzyme to achieve the same extent of ligations cohesive-end DNA fragments.- Blunt-end ligation may be enhanced by addition of PEG 4000 (10% w/v) final concentration or hexamine chloride, or by reducing the ATP concentration to 50µM.- To dilute T4 DNA Ligase for subsequent storage at -20°C the usage of a storage buffer containing 50% glycerol is recommended.- To dilute T4 DNA Ligase for immediate use, usage of 1X Reaction Buffer is recommended.Applications • Cloning of restriction enzymes generated DNA fragments • Cloning of PCR products • Connection of double-stranded oligonucleotide linkers or adapters with DNA • site-specific mutagenesis • Amplified fragment length polymorphism (AFLP) • Nicking repair in duplex DNA, RNA or DNA/RNA hybrids • Self-circulation of linear DNA.Definition of T4 Ligase activity2 different definitions of T4 Ligase activities are used!One Weiss unit is defined as the amount of enzyme required to convert 1 nmol of 32P-labeled inorganic pyrophosphate into Norit adsorbable material in 20 minutes at 37°C, using specified reaction conditions (Weiss, B., et al., (1968) J. Biol. Chem., 243, 4543). Norit is a type of activated carbon.A Cohesive End Unit is defined as the amount of enzyme required to give 50% ligation of Hind III fragments of lambda DNA (5´ DNA termini concentration of 0.12µM (300µg/mL)) in 20µL of 1X T4 DNA Ligase Buffer in 30 minutes at 16°C. The conversion factor between the two activities is: One Cohesive End Ligation units (CEL units) corresponds to 0.015 Weiss units. One Weiss unit therefore corresponds to 66.67 Cohesive End Ligation units (CEL units).

TAE buffer is used for nucleic acid electrophoresis on agarose gels under low voltage conditions. Ref.: Loening U.E. (1967) Biochem. J., 102, 251, Ogden R.C. and Adams D.A. (1987) Methods Enzymol., 152, 61. TAE buffer is the most commonly used running buffer for agarose gels. Originally, this buffer system was developed for polyacrylamide gel electrophoresis with a slightly different composition (40mM Tris; 20mM; NaOAc; 2mM EDTA-Na2; pH7.8). For stabilizing the secondary structure of RNA, sodium acetate was included. Today, TAE is used in a modified composition (40mM Tris-acetate; 1mM EDTA-Na2; ~pH8.5). TAE has a lower buffering capacity than TBE, but double-stranded, linear DNA migratesapproximately 10% faster through TAE than TBE with the same resolution. The resolution of supercoiled DNA is better in TAE than TBE. Because of its low buffering capacitiy, it may become exhausted during long periods of time at high current. Therefore TAE should be replaced during extended electrophoresis or should be recirculated. An advantage of TAE over TBE is the absence of interactions with agarose, resulting in a higher yield of nucleic acids in preparative agarose gel electrophoresis. Usually TAE is made up as a 50X concentrated stock solution and employed in an 1X or 0,5X working concentration.

TAE buffer is used for nucleic acid electrophoresis on agarose gels under low voltage conditions. Ref.: Loening U.E. (1967) Biochem. J., 102, 251, Ogden R.C. and Adams D.A. (1987) Methods Enzymol., 152, 61. TAE buffer is the most commonly used running buffer for agarose gels. Originally, this buffer system was developed for polyacrylamide gel electrophoresis with a slightly different composition (40mM Tris; 20mM; NaOAc; 2mM EDTA-Na2; pH7.8). For stabilizing the secondary structure of RNA, sodium acetate was included. Today, TAE is used in a modified composition (40mM Tris-acetate; 1mM EDTA-Na2; ~pH8.5). TAE has a lower buffering capacity than TBE, but double-stranded, linear DNA migratesapproximately 10% faster through TAE than TBE with the same resolution. The resolution of supercoiled DNA is better in TAE than TBE. Because of its low buffering capacitiy, it may become exhausted during long periods of time at high current. Therefore TAE should be replaced during extended electrophoresis or should be recirculated. An advantage of TAE over TBE is the absence of interactions with agarose, resulting in a higher yield of nucleic acids in preparative agarose gel electrophoresis. Usually TAE is made up as a 50X concentrated stock solution and employed in an 1X or 0,5X working concentration.

Contents of 1 pouch dissolved in deionized water and made up to 500mL/1000mL yields: 2.0M Tris acetate buffer, 0.05M EDTA, pH8.3 at 25°C. In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis.TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels. TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide. Medicago’s TBE and TAE buffers are supplied as a pre-weighed powder mix in sealed pouches giving 1000mL of 1X, 5X or 10X Tris-borate-EDTA buffer or 50X Tris-acetate-EDTA buffer with pH 8.3 at 25°C.

This buffer is the most widely used buffer for electrophoresis on agarose or acrylamide gels, it is particularly well suited for high-voltage long migration conditions. pH8.3 +/- 0.2. Delivery in PE bottles / canister.

This buffer is the most widely used buffer for electrophoresis on agarose or acrylamide gels, it is particularly well suited for high-voltage long migration conditions. pH8.3 +/- 0.2.

Genaxxon’s Unique Dual Index Set A is designed for precise and efficient sample preparation in Next Generation Sequencing on Illumina platforms. This set includes 48 unique dual index primer pairs (UDI Primers 1-48), ideal for medium-scale to large projects.Key Features:High Ligation Efficiency: Maximizes library output with minimal adapter dimer formation, ensuring superior performance.Enhanced Detection Accuracy: Effectively prevents data crosstalk, crucial for low-frequency mutation detection and low-abundance pathogen analysis.Truncated Adapters: Includes 15 μM truncated adapters with an 8-nucleotide sample index length for accurate and reliable indexing.Applications: Ideal for DNA or RNA library preparation on Illumina platforms, perfectly compatible with the 96 reactions of (either 2xUDI Set A or UDI Set A combined with UDI Set B): Genaxxon DNA Library Prep Kit for Illumina (M4400) Genaxxon DNA Library Prep Kit PLUS for Illumina (M4401)

Genaxxon’s Unique Dual Index Set B is designed for precise and efficient sample preparation in Next Generation Sequencing on Illumina platforms. This set includes 48 unique dual index primer pairs (UDI Primers 49-96), ideal for medium-scale to large projects.Key Features:High Ligation Efficiency: Maximizes library output with minimal adapter dimer formation, ensuring superior performance.Enhanced Detection Accuracy: Effectively prevents data crosstalk, crucial for low-frequency mutation detection and low-abundance pathogen analysis.Truncated Adapters: Includes 15 μM truncated adapters with an 8-nucleotide sample index length for accurate and reliable indexing.Applications: Ideal for DNA or RNA library preparation on Illumina platforms, perfectly compatible with the 96 reactions of (either 2xUDI Set A or UDI Set A combined with UDI Set B): Genaxxon DNA Library Prep Kit for Illumina (M4400) Genaxxon DNA Library Prep Kit PLUS for Illumina (M4401)

Genaxxon NGSQuant dsDNA HS Assay Kit for Qubit is a highly efficient, fluorescence-based solution for rapid and sensitive quantification of double-stranded DNA (dsDNA). This kit provides accurate measurements for initial DNA concentrations ranging from 10pg/µL to 100ng/µL, making it ideal for a variety of applications, including next-generation sequencing (NGS) and DNA library quantification. With its easy-to-use format and compatibility with Qubit fluorometers, the kit delivers fast, reliable, and precise dsDNA measurements for laboratories focused on high-quality data. Highlights Fast and accurate quantificationPrecise dsDNA measurement for concentrations ranging from 10pg/µL to 100ng/µLSelective detectionHigh contaminant tolerance - Performs reliably even in the presence of salts, solvents, detergents, and proteins. Complete kit – Includes concentrated assay reagent, dilution buffer, and DNA standards. Optimized for NGS – Perfect for quantifying input DNA for next-generation sequencing and DNA library quantification. Applications The NGSQuant dsDNA HS Assay Kit can be used for:Quantification of input DNA for next-generation sequencing (NGS)DNA library quantificationSuitable for use with Qubit fluorometers for precise dsDNA measurements

Genaxxon NGSQuant 1X dsDNA HS Assay Kit is a rapid, highly sensitive, and accurate solution for the quantitative detection of double-stranded DNA (dsDNA) using fluorescence. This kit is specifically designed for dsDNA, offering excellent linearity in the range of 0.2 ng to 100 ng, with a quantitation range of 10 pg/µL to 100 ng/µL.The user-friendly kit provides a ready-to-use working solution that simplifies the dsDNA quantification process on Qubit® fluorometers or fluorescence microplate readers. It is an ideal choice for large-scale DNA sample quantification in next-generation sequencing (NGS) applications, including input DNA and DNA library quantification. Additionally, the kit demonstrates robust tolerance to common contaminants, such as proteins and salts, ensuring reliable results across various sample types.Highlights Rapid, highly sensitive, and accurate fluorescent detection of double-stranded DNA (dsDNA). Excellent linearity in the range of 0.2 ng to 100 ng. Quantitation range of 10 pg/µL to 100 ng/µL. Ready-to-use working solution for easy operation. Compatible with Qubit® fluorometers and fluorescence microplate readers. Tolerant to common contaminants such as proteins and salts. Applications The NGSQuant 1X dsDNA HS Assay Kit is suitable for: Large-scale DNA sample quantification for next-generation sequencing (NGS). Input DNA quantification for NGS workflows. DNA library quantification for various sequencing applications.

High-Purity Enzyme for In Vitro TranscriptionGNX T7 RNA Polymerase is a recombinant enzyme derived from bacteriophage T7 and expressed in Escherichia coli. It catalyzes the 5'→3' synthesis of RNA using double-stranded DNA containing a T7 promoter sequence (5'-TAATACGACTCACTATAGG-3') as a template and NTPs (ATP, CTP, GTP, UTP) as substrates for in vitro transcription.The polymerase is compatible with linear double-stranded DNA templates with either blunt ends or 5'-protruding ends. This makes it suitable for use with both linearized plasmid DNA and PCR products containing a T7 promoter.Supplied in a convenient liquid format, this enzyme is ideal for a variety of molecular biology applications such as RNA synthesis, RNA interference (RNAi), hybridization, in vitro translation, and the preparation of RNA probes.Key Features:High activity and specificity for T7 promoter sequencesSuitable for short and long RNA transcriptsCompatible with PCR products and linearized plasmids (blunt end or 5'-overhang)Liquid formulation for immediate useSynthesis principle:

Reliable RNA Stabilization for Optimal AnalysisThe Genaxxon Fix-it - Nucleic Acid Stabilization Reagent is a non-toxic reagent designed to stabilize and preserve RNA & DNA in biological samples such as tissues, cells, and blood. It rapidly penetrates the sample and effectively prevents DNA and RNA degradation by inhibiting Nuclease activity. This eliminates the need for immediate sample processing or storage in liquid nitrogen. Tissue samples can be collected and immersed in the Fix-it - Nucleic Acid Stabilization Reagent immediately after harvesting, ensuring high RNA quality and yield for subsequent isolation and analysis.AdvantagesReliable RNA stabilization – Protects RNA in tissues, cells, and blood by inhibiting RNases.Immediate protection – Quickly preserves RNA integrity after sample collection.Flexible storage – No need for immediate processing or freezing in liquid nitrogen.High-quality RNA – Ensures optimal yield for downstream applications.Safe and easy to use – Non-toxic, aqueous formulation for convenient handling.Highly effective - Stabilizes RNA for 1 day at 37°C, for 1 week at 25°C, for 1 month at +2°C to +8°C, or indefinitely at -20°C/-80°C.ApplicationsGene expression analysis – Provides high-quality RNA for RT-PCR and qPCR.RNA sequencing (RNA-Seq) – Preserves RNA integrity for transcriptome studies.Microarray analysis – Maintains RNA stability for accurate expression profiling.Biobanking – Enables long-term RNA storage without degradation.Field sample collection – Allows RNA preservation without immediate freezing.

Efficient in vitro Transcription with High RNA YieldThe T7 High Yield RNA Synthesis Kit provides an optimized transcription system for the efficient in vitro transcription of single-stranded RNA. Utilizing T7 RNA polymerase and a linear double-stranded DNA template containing the T7 promoter sequence, the kit enables the synthesis of 100–200 µg of RNA from just 1 µg of DNA template.Designed for both short and long RNA transcripts, this kit supports the incorporation of modified nucleotides, such as biotin- or dye-labeled NTPs, allowing for the generation of labeled RNA for downstream applications.Applications include:RNA structure and function studiesRNase protection assaysProbe hybridizationRNA interference (RNAi)MicroinjectionIn vitro translationKey benefits: High RNA yield (up to 200 µg per reaction) Suitable for long and short transcripts Easy incorporation of labeled nucleotidesReliable and reproducible results Versatile use in molecular biology and genetic research Synthesis principle:The ideal tool for researchers in molecular biology, genetics, and biotechnology.

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.89M Tris-borate, 0.02M EDTA, pH8.3 at 25°C (pH value of a 1X buffer solution).The buffer does not require the use of calibration or measuring equipment. No time-consuming or costly validation procedures are needed. Simply dissolve the powder and use it!Instructions for use:Transfer the contents of one sachet of TAE or TBE buffer powder into a beaker placed on a magnetic stirrer. Add 300mL of deionised water and stir the solution for a few minutes. Then make up the volume to 1000mL with deionised water. Continue stirring until everything has completely dissolved. The buffer solution is now ready for use! You will find more ready-to-use buffers here: ready-to-use buffers >

DescriptionRNase-free DNAse I is mainly used to remove DNA from RNA preparations. E.g. for experiments like RT-PCR, RT-qPCR or other applications that should be free of DNA. Since no purification method for RNA removes 100% of the DNA, it is advisable to remove DNA using DNAse I prior to any RT-PCR. A simple 15-minute digestion at room temperature reliably removes all contaminating DNA. DNase I is inactivated by adding the stop solution (1µL of a 50mM EDTA solution to 10µL of the DNA digest solution) and subsequent heating. Since heat also denatures RNA, the batch can then be used immediately for reverse transcription. Our RNase-free DNAse I is a recombinantly derived DNase I. Therefore, there are no problems with possible traces of animal origin.

Genaxxon’s Unique Dual Index Midi Set is designed for precise and efficient sample preparation in Next Generation Sequencing on Illumina platforms. This set includes 24 unique dual index primer pairs (UDI Primers 1-24), ideal for small to medium-scale projects.Key Features:High Ligation Efficiency: Maximizes library output with minimal adapter dimer formation, ensuring superior performance.Enhanced Detection Accuracy: Effectively prevents data crosstalk, crucial for low-frequency mutation detection and low-abundance pathogen analysis.Truncated Adapters: Includes 15 μM truncated adapters with an 8-nucleotide sample index length for accurate and reliable indexing.Applications: Ideal for DNA or RNA library preparation on Illumina platforms, perfectly compatible with the 24 reactions of: Genaxxon DNA Library Prep Kit for Illumina (M4400) Genaxxon DNA Library Prep Kit PLUS for Illumina (M4401)

Genaxxon’s rRNA Depletion Module offers a precise and efficient solution for removing ribosomal RNA (rRNA) from total RNA samples, enhancing the quality of RNA for downstream applications such as sequencing and transcriptome analysis. Tailored for prokaryotic RNA, this module effectively targets and depletes rRNA, reducing its interference in RNA sequencing results.Key Features:Specifically designed to deplete rRNA from prokaryotic RNA samples.Efficient Removal: Significantly reduces rRNA levels, enriching non-ribosomal RNA for accurate analysis.Streamlined Process: Simplifies sample preparation, ensuring high-quality RNA for subsequent applications.Rigorous Quality Control: Each batch undergoes thorough testing to ensure consistent performance. Applications: RNA sequencing for prokaryotes Transcriptome analysis of bacterial and other prokaryotic samplesSeamless Compatibility:Fully compatible with Genaxxon’s FAST RNA-seq Library Prep Kit, offering an integrated workflow for RNA capture and library preparation.

Genaxxon’s 2X Oligo (dT)25 Capture Beads offer a reliable and efficient solution for isolating poly(A) RNA from purified total RNA samples. This capture method is based on the binding between the poly(A) tail at the 3' end of mRNA and the Oligo d(T) sequence on the magnetic microsphere. With a streamlined protocol, the capture process is completed in under 1 hour, making it ideal for fast and accurate RNA preparations. Key Features:High Specificity: Ensures targeted capture of poly(A) RNA from total RNA samples.Fast Protocol: Achieve RNA isolation in under 1 hour.Consistent Results: Every kit batch undergoes rigorous quality control and is verified through library preparation and sequencing, ensuring reliable and reproducible results.Complete Kit: Includes all required reagents, optimized for poly(A) RNA isolation.Applications: RNA purification for downstream applications such as RNA sequencing, RT-PCR, and gene expression analysis. Poly(A) RNA enrichment from total RNA samples for high-throughput sequencing.Seamless Compatibility: Fully compatible with Genaxxon’s FAST RNA-seq Library Prep Kit, offering an integrated workflow for RNA capture and library preparation.

Dextran sulfate is a polyanion produced by esterification of dextran with chlorosulphonic acid. Dextran sulfate 500 is a dextran with an average molecular weight of approx. 500,000 Daltons. Anionic dextrans such as dextran sulfate (10% in hybridisation buffer) accelerate the hybridisation of nucleic acids by a factor of approx. 10.Synonyms: 6-O-(6-O-beta-D-Glucopyranosyl-beta-D-glucopyranosyl)-D-glucose, Dextran 500, Manninotriose Dextran sulfate is routinely used forSelective precipitation of lipoproteinsProbe hybridization of membrane-immobilized DNARelease of DNA from DNA-histone complexesInhibition of RNA binding to ribosomes

The Genaxxon NGSClone PCR Mastermix (2x) combines high-fidelity DNA polymerase with antibody-mediated Hot-Start technology for precise, fast, and reliable PCR results. Perfect for challenging templates such as GC-rich DNA, cDNA, or complex plant and animal samples. This ready-to-use master mix contains all essential components – just add primers and template – making it ideal for high-fidelity PCR and NGS library preparation.NGSClone PCR Mastermix (2x) – High-Fidelity & Hot-Start for Accurate PCRMaximum accuracy for demanding PCR applicationsThe Genaxxon NGSClone PCR Mastermix (2x) delivers outstanding performance by combining a novel high-fidelity DNA polymerase with antibody-mediated Hot-Start technology in a convenient ready-to-use PCR master mix. With up to 120× higher fidelity compared to standard Taq polymerases, it ensures highly accurate amplification even for GC-rich templates. Ideal for PCR in NGS workflows, cDNA amplification, and plant or animal DNA analysis.Key Features of the NGSClone PCR Mastermix (2x)Exceptional accuracy – High-fidelity DNA polymerase with strong 3’-5’ exonuclease activity (proofreading) for error-free amplification Fast & efficient PCR – Engineered enzyme structure with enhanced domain for high processivity and faster extension rates Fast amplification – achieves target amplification in just 10–15 seconds per kbHot-Start precision – Antibody blocking keeps the enzyme inactive at room temperature, preventing non-specific amplification and improving specificity Broad applicability – Suitable for GC-rich DNA, cDNA, plant and animal templates, and other difficult targets Easy workflow – Contains polymerase, dNTPs, and optimized buffer; just add primers and DNA Benefits in Your Lab Saves time and reduces pipetting stepsMinimizes cross-contamination Delivers high reproducibility Remains stable after repeated freeze-thaw cycles Applications High-fidelity PCR for error-free cloning PCR of GC-rich templates cDNA amplification NGS library preparation Standard PCR requiring high accuracy Genaxxon’s NGSClone PCR Mastermix (2x) – for fast, precise, and reproducible PCR results with maximum convenience.The Genaxxon NGSClone PCR Mastermix Red (2x) is also available with a red loading dye.

The enzyme Lysozyme (Muramidase) affects the cell walls of bacteria. Thereby the extraction efficiency of proteins or nucleic acids is significantly improved. Genaxxon Lysozyme from chicken egg is available as lyophilized powder. Lysozyme preferentially hydrolyses the β-1,4-glycosidic binding between N-Acetyl muraminic acid and N-Acetyl glucosamine, a component of the proteoglycan-cell wall of certain microorganisms. The enzyme is present in many organisms. In molecular biology, the enzyme from chicken white egg is used to lyse E. coli for the isolation of plasmid-DNA. The lysis of E.coli is improved by the combined addition of both lysozyme and and a nuclease such as DNase I (M3028) >. The working concentration is 200-300 μg/µL. To increase the plasmid yield (approx. 5-10%), lysozyme may be added. Another application is the lysis of bacteria for the preparation of bacterial RNA. In this case the working concentration is 40μg/mL (stock solution 50mg/mL). Lysozyme from chicken egg is most effective for the lysis of gram-positive bacteria. Besides this, it facilitates the lysis of gram-negative bacteria such as Salmonella and Shigella. Lyophilized, white powder Enzyme for lysis of bacterial cell walls Improvement of protein purification from inclusion bodies Activity: min. 20.000 U/mg (pH: 6.25) Lysozyme will be inhibited by surfactants like SDS (M3292) > or alcohols and fatty acids, Imidazole (M6033) > and Indol-derivatives. Stability: The lyophilized powder of lysozyme is stable for many years at +2°C to +8°C. In solution, the stability at pH values from 4 to 5 at +2°C to +8°C is several weeks and at room temperature several days. pH-optimum is at 9.2, the isoelectric point at 11.0.

Hyaluronidase isolated from sheep testes. The mammalian glycolytic hyaluronidase (EC 3.2.1.35) catalyzes the hydrolysis of the 1-4 bond between N-acetyl-D-glucosamine and D-glucuronic acid in hyaluronic acid. It also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin sulfates A and C, and dermatan. pH-Optimum of the enzyme: 7.0-8.0. Stock solution: 1-300mg/mL in phosphate buffer or water. Working solution: 0.1-10mg/mL. Synonyms: Hyaluronoglucosidase, Hyaluronate 4-glycanohydrolase, Mucinase.

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.445M Tris-borate, 0.01M EDTA, pH8.3 at 25°C. In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis. TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels. TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide. Medicago’s TBE and TAE buffers are supplied as a pre-weighed powder mix in sealed pouches giving 1000mL of 1X, 5X or 10X Tris-borate-EDTA buffer or 50X Tris-acetate-EDTA buffer with pH 8.3 at 25°C. You will find more ready-to-use buffers here: ready-to-use buffers >

Lysozyme (Muramidase) preferentially hydrolyses the β-1,4-glycosidic binding between N-Acetyl muraminic acid and N-Acetyl glucosamine, a component of the proteoglycan-cell wall of certain microorganisms. The enzyme is present in many organisms. In molecular biology, the enzyme from chicken white egg is used to lyse E. coli for the isolation of plasmid-DNA. The working concentration is 200-300 μg/µL. To increase the plasmid yield (approx. 5-10%) lysozyme may be added. Another application is the lysis of bacteria for the preparation of bacterial RNA. In this case the working concentration is 40μg/mL (stock solution 50mg/mL). Stability: The lyophilized powder of lysozyme is stable for many years at +2°C to +8°C. In solution, the stability at pH values from 4 to 5 at +2°C to +8°C is several weeks and at room temperature several days. pH-optimum is at 9.2, the isoelectric point at 11.0. Lysozyme will be inhibited by surfactants like SDS or alcohols and fatty acids, imidazole and indol-derivatives.