Cell Biology

Trypsin is a mixture of proteases isolated from porcine pancreas. 0.05% Trypsin is produced by dilution of Trypsin in PBS. Due to its protease activities, trypsin solutions are widely used for cell dissociation, routine cell culture passaging, or primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. For this Genaxxon offers a variety of different Trypsin solutions with and without EDTA. Activity of the used Trypsin: 1g of Trypsin digests 250g of Casein substrate. Minimal order size: 5 x C4261.0100 (100mL) or 2 x C4261.0500 (500mL).

Trypsin is a mixture of proteases isolated from porcine pancreas. 0.05% Trypsin is produced by dilution of Trypsin in PBS. Due to its protease activities, trypsin solutions are widely used for cell dissociation, routine cell culture passaging, or primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. For this Genaxxon offers a variety of different Trypsin solutions with and without EDTA. Activity of the used Trypsin: 1g of Trypsin digests 250g of Casein substrate. Minimal order size: 5 x C4260.0100 (100mL) or 2 x C4260.0500 (500mL).

Trypsin is a mixture of proteases isolated from porcine pancreas. 0.05% Trypsin is produced by dilution of Trypsin in PBS. Due to its protease activities, trypsin solutions are widely used for cell dissociation, routine cell culture passaging, or primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. For this Genaxxon offers a variety of different Trypsin solutions with and without EDTA. Activity of the used Trypsin: 1g of Trypsin digests 250g of Casein substrate. Minimal order size: 5 x C4260.0100 (100mL) or 2 x C4260.0500 (500mL).

Trypsin is a mixture of proteases isolated from porcine pancreas. 0.25% Trypsin is produced by dilution of Trypsin in HBSS. Due to its protease activities, trypsin solutions are widely used for cell dissociation, routine cell culture passaging, or primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. For this Genaxxon offers a variety of different Trypsin solutions with and without EDTA. Activity of the used Trypsin: 1g of Trypsin digests 250g of Casein substrate. Minimal order size: 5 x C4288.0100 (100mL) or 2 x C4288.0500 (500mL).

Trypsin is a mixture of proteases isolated from porcine pancreas. 0.25% Trypsin is produced by dilution of Trypsin in PBS. Due to its protease activities, trypsin solutions are widely used for cell dissociation, routine cell culture passaging, or primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. For this Genaxxon offers a variety of different Trypsin solutions with and without EDTA. Activity of the used Trypsin: 1g of Trypsin digests 250g of Casein substrate. Minimal order size: 5 x C4259.0100 (100mL) or 2 x C4259.0500 (500mL).

Trypsin is a mixture of proteases isolated from porcine pancreas. 0.25% Trypsin is produced by dilution of Trypsin in PBS. Due to its protease activities, trypsin solutions are widely used for cell dissociation, routine cell culture passaging, or primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. For this Genaxxon offers a variety of different Trypsin solutions with and without EDTA. Activity of the used Trypsin: 1g of Trypsin digests 250g of Casein substrate. Minimal order size: 5 x C4267.0100 (100mL) or 2 x C4267.0500 (500mL).

Trypsin is a mixture of proteases isolated from porcine pancreas. 0.25% Trypsin is produced by dilution of Trypsin in PBS. Due to its protease activities, trypsin solutions are widely used for cell dissociation, routine cell culture passaging, or primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. For this Genaxxon offers a variety of different Trypsin solutions with and without EDTA. Activity of the used Trypsin: 1g of Trypsin digests 250g of Casein substrate. Minimum order size is 5 x C4262.0100 (100mL) or 2 x C4262.0500 (500mL).

Trypsin is a mixture of proteases isolated from porcine pancreas. 0.5% Trypsin is produced by dilution of Trypsin in PBS. Due to its protease activities, trypsin solutions are widely used for cell dissociation, routine cell culture passaging, or primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. For this Genaxxon offers a variety of different Trypsin solutions with and without EDTA. Activity of the used Trypsin: 1g of Trypsin digests 250g of Casein substrate. Minimal order size: 5 x C4261.0110 (100mL) or 2 x C4261.0510 (500mL).

Trypsin is a mixture of proteases isolated from porcine pancreas. 2.5% Trypsin is produced by dilution of Trypsin in PBS. Due to its protease activities, trypsin solutions are widely used for cell dissociation, routine cell culture passaging, or primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. For this Genaxxon offers a variety of different Trypsin solutions with and without EDTA. Activity of the used Trypsin: 1g of Trypsin digests 250g of Casein substrate. Minimal order size: 5 x C4287.0100 (100mL) or 2 x C4287.0500 (500mL).

200mM L-glutamine solution (100-times) for cell culture applications.L-Glutamine is an amino acid that is essential for cell culture. L-Glutamine is used in the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, and other amino acids, as well as in protein synthesis and glucose production. Unlike most other amino acids, L-glutamine is not stable in solution. The rate at which degradation proceeds is a function of time, temperature, and pH. Genaxxon L-glutamine is a ready-to-use 200mM stock solution that acts as a cell culture supplement. The optimal concentration is dependent upon the cell type and medium used to culture the cells, but generally falls in the range of 2–6mM. Genaxxon offers also a stable glutamine (L-alanyl-L-glutamine) > which is not degraded in solution and can be used by cells instead of L-glutamine.

Accutase® as a cell detachment solution of proteolytic and collagenolytic enzymes useful for the routine detachment of cells from standard tissue culture plastic ware and adhesion coated plastic ware. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue. It is much better suited for cell detachment than trypsin. Proven effective in detaching primary fibroblasts, endothelial cells, neurons, tumor cell lines, and insect cells. Accutase® does not contain mammalian or bacterial derived products. For that reason Accutase® virus or endotoxin contaminations are excluded. Once thawed Accutase® can be used for about 1 week if stored at +2°C to +8°C. Shelf-life at -20°C is 12 months. Note: Accutase is a registered trademark of Innovative Cell Technologies, Inc.

Synonym: Adenosin, Adenosine, 9-β-D-Ribofuranosyladenine, Adenine riboside, Adenine-9-β-D-ribofuranoside, D-Adenosine

Overview:The ADSC Exosome HY Medium is a specially designed culture medium for the isolation and cultivation of exosomes from adipose-derived stem cells (ADSC). This medium promotes efficient exosome production and ensures optimal conditions for their collection and application in research and therapy.Product Details:Formulation: Specifically tailored for the cultivation of adipose-derived stem cells and the isolation of exosomes.Components: Contains all necessary nutrients, growth factors, and supplements to ensure healthy cell growth and high exosome production.Application: Ideal for research applications, including cell communication studies, regenerative medicine, and therapeutic developments.Compatibility: Works excellently with standard cell culture protocols and equipment. Stability: The medium is stable at +2°C to +8°C for 2 years.Benefits: Efficient Exosome Production: Optimized formulation promotes high exosome yield. Quality and Consistency: Consistent results through standardized ingredients and production. Application:For exosome production, we recommend culturing ADSCs in dishes or flasks under regular culture conditions up to 90% confluence. Then remove the original culture medium and wash the cells with PBS and ADSC Exosome HY medium to remove residues of the original medium. Then the ADSC cells are cultured in ADSC Exosome HY medium for 3-4 days before the conditioned medium can be used for exosome isolation.Picture 1: Flow Cytometry - Verification of CD9, CD63, CD81 surface markerPicture 2: Yield comparison between standard cell culture media and ADSC Exosome HY MediumProduced by Genaxxon bioscience GmbH, founded in 2002 by Dr. Norbert Tröndle to provide reliable products for PCR and custom PCR and cell culture media formulations, ADSC Exosome HY Medium offers a dependable and efficient solution for your cell culture and exosome research needs. Optimize your experiments and achieve consistent, high-quality results with a medium designed specifically for your requirements.Conventional Methods for Exosome Extraction and IsolationWorkflow with Magnetic Beads for Exosome Purification

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle’s salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Alpha MEM Eagle (with Earles salts) without Glutamine, without Phenol Red, without Ribonucleosids and Deoxyribonucleosides, with 2.2g/L NaHCO3, sterile filtered. Special preparation: Minimum order size: 20 x 500mL. If you wish, you can choose all incredients as you need without changing the price per bottle. Just let us know your needs.

Alpha MEM medium, with sodium bicarbonate, without L-Glutamine, without ribonucleosides and without deoxyribonucleosides, with Glucose, sterile-filtered, for cell culture applications. Alpha medium is a modified MEM medium, originally developed to grow Chinese hamster kidney cells in vitro. Alpha medium supports the growth of bone marrow cells under both mononolayer and suspension culture conditions. The medium is also suitable for amniotic fluid cells in chromosome analysis. Another widely used supplement modification are nucleosides. C4051 Alpha MEM Eagle (with Earles Salts) is without L-Glutamine, without Ribonucleosides and without Deoxyribonucleosides, with Glucose, with 2.2g/L NaHCO3 and is sterile filtered.

Alpha MEM Eagle medium, without nucleoside, without Arginine, without Lysine, with Glucose, without Hepes, with Phenol red, with 2.2g/L sodium bicarbonate, sterile-filtered, for cell culture applications.Special preparation. Minimal order size: 20 x 500mL. Alpha medium is a modified MEM medium, originally developed to grow Chinese hamster kidney cells in vitro. Alpha medium supports the growth of bone marrow cells under both mononolayer and suspension culture conditions. The medium is also suitable for amniotic fluid cells in chromosome analysis. Another widely used supplement modification are nucleosides. Genaxxon offers a variety of cell culture media modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com. Genaxxon offers also other products as our broad range of Antibiotcs > - Amphotericin B > - Antibiotic Antimycotic Solution > - Doxycycline > - Geneticin disulfate (G418) > - Gentamycin sulfate > - Hygromycin B > - Kanamycin > - Penicillin/Streptomycin >.

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle’s salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Alpha MEM Eagle (with Earles salts) with Glutamine, without Glucose, with Ribonucleosids and Deoxyribonucleosides, with 2.2g/L NaHCO3, sterile filtered. Special preparation. Order size: min. 20 x 500mL. If you wish, you can choose all incredients as you need without changing the price per bottle. Just let us know your needs.

Alpha MEM medium, with sodium bicarbonate, with L-Glutamine, with Glucose, with ribonucleosides and deoxyribonucleosides, sterile-filtered, for cell culture applications. Alpha medium is a modified MEM medium, originally developed to grow Chinese hamster kidney cells in vitro. Alpha medium supports the growth of bone marrow cells under both mononolayer and suspension culture conditions. The medium is also suitable for amniotic fluid cells in chromosome analysis. Another widely used supplement modification are nucleosides. C4003 Alpha MEM Eagle (with Earles Salts) is with L-Glutamine, with Glucose, with Ribonucleosides and Deoxyribonucleosides, with 2.2g/L NaHCO3 and is sterile filtered.

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle’s salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Alpha MEM Eagle (with Earles salts) with Glutamine, with Glucose, with Ribonucleosids and Deoxyribonucleosides, with 2.2g/L NaHCO3, sterile filtered. Special preparation. Order size: min. 20 x 500mL. If you wish, you can choose all incredients as you need without changing the price per bottle. Just let us know your needs.

Alpha MEM Eagle medium, with Nucleosides, without Amino Acids, with Glucose, without Hepes, with 2.2g/L sodium bicarbonate, sterile-filtered, for cell culture applications.Special preparation. Minimal order size: 20 x 500mL. Alpha medium is a modified MEM medium, originally developed to grow Chinese hamster kidney cells in vitro. Alpha medium supports the growth of bone marrow cells under both mononolayer and suspension culture conditions. The medium is also suitable for amniotic fluid cells in chromosome analysis. Another widely used supplement modification are nucleosides.

Alpha MEM medium, with sodium bicarbonate, with L-glutamine, with Glucose, without ribonucleosides and without deoxyribonucleosides, sterile-filtered, for cell culture applications. Alpha medium is a modified MEM medium, originally developed to grow Chinese hamster kidney cells in vitro. Alpha medium supports the growth of bone marrow cells under both mononolayer and suspension culture conditions. The medium is also suitable for amniotic fluid cells in chromosome analysis. Another widely used supplement modification are nucleosides. C4006 Alpha MEM Eagle (with Earles Salts) is with L-Glutamine, with Glucose but without Ribonucleosides and without Deoxyribonucleosides, with 2.2g/L NaHCO3 and is sterile filtered.

Amphotericin B is a polyene antifungal that is used in cell culture to suppress fungal and yeast contamination, but does not act on bacteria. Addition of deoxycholate in phosphate buffer improves water solubility. For use: dilute 1: 100 (2.5μg/mL). Contains sodium deoxycholate. Amphotericin B is almost insoluble in water at pH6-7, at pH2 and pH11 soluble only with 100µg/mL. In DMSO 30-40mg/mL can be dissolved. Genaxxon bioscience offers a broad range of Antibiotics and Antimycotics > as powder or as solutions. Fungizone is a registered trademark of E.R. Squibb.

Antibiotic Antimycotic Solution (100X) for prevention of contamination by bacteria, yeasts and moulds. Penicillin G from Penicillium notatum and streptomycin from Streptomyces griseus prevent together against gram-positive and gram-negative bacteria bacterial contamination of cell cultures. Penicillin G acts by interfering directly with turnover of bacterial cell wall and indirectly by triggering release of enzymes that alter the cell wall. Streptomycin works by attaching to the 30S subunit of the bacterial ribosome, which leads to an inhibition of protein synthesis and the subsequent death of bacteria. Amphotericin B interferes with fungal membrane permeability by forming channels in the membranes and causing small molecules to leak out. Antimicrobial spectrum: Gram-negative bacteria, Gram-positive bacteria, fungi and yeasts. Recommended for use in cell culture applications at 10mL/L. Genaxxon bioscience offers a broad range of Antibiotics and Antimycotics as powder or solutions.The minimum order quantity is 5 × M3140.0100 (100 mL).

Antide acetate is a peptide which acts as an LHRH antagonist and can be utilized by an animal’s body to represses FSH and LH releases that stem from the pituitary gland. Synthetic versions of this chemical have been developed for use/controlling of these reactions in a laboratory setting, both as a means to determine a potential medical use for antide acetate and to help researchers better understand the role of this chemical in an animal’s body when it is naturally produced. Antide acetate that is prepared for laboratory use is provided as a lyophilized sterile powder that can be reconstituted with an aqueous solution as necessary for the given application. Researchers are encouraged to seek out a supply that is prepared without additives, to ensure accurate and reliable testing results once the chemical is in use. Synonym: Ac-D-2-Nal-4-chloro-D-Phe-β-(3-pyridyl)-D-Ala-Ser-Lys(nicotinoyl)-D-Lys(nicotinoyl)-Leu-Lys(isopropyl)-Pro-D-Ala-NH₂ acetate salt.

Apramycin is produced from Streptomyces tenebrarius. It is used to study antibiotic resistance as well as protein synthesis translocation-step inhibition in bacteria and prokaryotes. Apramycin is an aminoglycoside antibioticum and has a bactericidal action against many gram-negative bacteria. Apramycin inhibits protein synthesis by blocking translocation. It is also able to bind to the eukaryotic decoding site. At low concentrations it inhibits elongation and induces misreading of mRNA during protein synthesis. Apramycin is a structurally unique antibioticum that contains a bicyclic sugar moiety and a monosubstituted deoxystreptamine. Apramycin can only be acetylated by AAC-(3)IV and as a consequence of this enzymatic modification, the antibioticum is unable to enter the cell to bind to its ribosomal target. Apramycin (also Nebramycin II) is an aminoglycoside antibiotic used in veterinary medicine. It is used in pigs, sheep, cows, chicken and rabbits.

Bacitracin from Bacillus licheniformis consists of several peptides (A, B, C, D, E, F1-3), bacitracin A, a cyclic dodecapeptide, being the most important component with approximately 70%. For its bactericidal action, the presence of divalent cations (eg, zinc) is required, with which Bacitracin forms stable complexes. Like Vancomycin, it inhibits the biosynthesis of the cell wall (especially gram-positive bacteria and cocci) by binding to bactoprenylpyrophosphate. Bacitracin is very stable as a dry substance (moisture content <1%) with a shelf life of about 15 months at room temperature and is stable for about 15 months. At higher temperatures (>55°C) the antibiotic is unstable. Bacitracin is readily soluble in water or alcohols (1g/mL) and forms stable salts with zinc. In aqueous solutions, it is relatively stable in the acidic pH range (pH4-5) and relatively rapidly inactivated in the range above pH5 (pH5 - 9) at 25°C. Above pH8 it is not durable at all. In solution, 10% of the activity is lost at +2°C to +8°C within about 2 months.

Basal Medium (Eagle) with EBSS, 1.0g/L glucose, without NaHCO3.Special preparation. Minimal order size: 20 x 500mL. In the fifties of the last century it became clear that mammalian cells need not only the 10 essential amino acids, but also Cystine, Tyrosine and Glutamine. In addition to these three amino acids, BME includes also eight B-vitamins. Originally BME was used for the cultivation of murine L-cells and HeLa-cells. With its many variations it is used in many fields of cell culture and applications today. Along with the cultivation of normal mammalian cells BME is very suitable for transformed cells. BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). As this BME uses a sodium bicarbonate buffer system (2.2g/L), a 5–10% CO2 environment is necessary to maintain physiological pH. Genaxxon offers a variety of cell culture media modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com. Genaxxon offers also other products as our broad range of peptides from stock > for your research. For example: Virus Epitope Peptides (CMV >, EBV >, HBV >, HIV >, etc.); Alzheimer Peptides; Cancer Research (Melan A (MART1) >, MAGE >, HER-2 >, p53 >); Cell Penetrating Peptides (Arg9 >, CyLoP-1 >, HIV-1 TAT >) and others as PADRE >; PRAME (100-108) HLA-A*02:01 >; MBP (1-11) human >; CD20 (188-196) HLA-A*02:01 >.

Basal Medium (Eagle) with EBSS, 1.0g/L glucose, with 2.2g/L NaHCO3, without Phenol red. Special preparation. Minimal order size: 20 x 500mL. In the fifties of the last century it became clear that mammalian cells need not only the 10 essential amino acids, but also Cystine, Tyrosine and Glutamine. In addition to these three amino acids, BME includes also eight B-vitamins. Originally BME was used for the cultivation of murine L-cells and HeLa-cells. With its many variations it is used in many fields of cell culture and applications today. Along with the cultivation of normal mammalian cells BME is very suitable for transformed cells. BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). As this BME uses a sodium bicarbonate buffer system (2.2g/L), a 5–10% CO2 environment is necessary to maintain physiological pH. Genaxxon offers a variety of cell culture media modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com. Genaxxon offers also other products as our broad range of peptides from stock > for your research. For example: Virus Epitope Peptides (CMV >, EBV >, HBV >, HIV >, etc.); Alzheimer Peptides; Cancer Research (Melan A (MART1) >, MAGE >, HER-2 >, p53 >); Cell Penetrating Peptides (Arg9 >, CyLoP-1 >, HIV-1 TAT >) and others as PADRE >; PRAME (100-108) HLA-A*02:01 >; MBP (1-11) human >; CD20 (188-196) HLA-A*02:01 >.

Basal Medium (Eagle) with EBSS without Glutamine, without Glucose, with Phenol red, with 2.2g/L NaHCO3.  In the fifties of the last century it became clear that mammalian cells need not only the 10 essential amino acids, but also Cystine, Tyrosine and Glutamine. In addition to these three amino acids, BME includes also eight B-vitamins. Originally BME was used for the cultivation of murine L-cells and HeLa-cells. With its many variations it is used in many fields of cell culture and applications today. Along with the cultivation of normal mammalian cells BME is very suitable for transformed cells. BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). As this BME uses a sodium bicarbonate buffer system (2.2g/L), a 5–10% CO2 environment is necessary to maintain physiological pH. Genaxxon offers a variety of cell culture media modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com. Genaxxon offers also other products as our broad range of peptides from stock > for your research. For example: Virus Epitope Peptides (CMV >, EBV >, HBV >, HIV >, etc.); Alzheimer Peptides; Cancer Research (Melan A (MART1) >, MAGE >, HER-2 >, p53 >); Cell Penetrating Peptides (Arg9 >, CyLoP-1 >, HIV-1 TAT >) and others as PADRE >; PRAME (100-108) HLA-A*02:01 >; MBP (1-11) human >; CD20 (188-196) HLA-A*02:01 >.

Group of glycopeptide antibiotics from Streptomyces verticillus with antineoplastic properties by inhibition of DNA synthesis. Bleomycin sulfate is a mixture of bleomycin A2 and bleomycin B2 as the major components. Bleomycin sulfate occurs as a cream-colored, amorphous powder and is very soluble in water and sparingly soluble in alcohol. The potency of bleomycin is assayed microbiologically, the activity of 1 unit of bleomycin is equivalent to the activity of 1mg of bleomycin A2 reference standard. For chemistry and action of bleomycin, see: Umezaw, H. (1980) Med. Chem. 16, 147-66.

Caesium chloride for density centrifugation. Ideal for the isolation of highly pure RNA without contamination with RNase, or other proteins and DNA. Ref.: Miller H. (1987) Methods Enzymol., 152, 145, Dorin M. and Bornecque C.A. (1995) BioTechniques, 18,90.

CMRL-1066 with L-Glutamine, without Phenol red, with 2.2g/L NaHCO3, sterile filtered. CMRL is a nucleoside and vitamin-rich medium. In the past it was developed to clone monkey-kidney cells and as long time culture medium for L-cells. It is sutiable for many types of human and monkey cells and also for other mammalian cells, especially by using horse and foetal bovine serum. This medium is a special preparation and only available in a pack size of 20 x 500mL. If you wish, you can choose all incredients as you need without changing the price per bottle. Just let us know your needs. Genaxxon offers also other products as our broad range of peptides from stock > for your research. For example: Virus Epitope Peptides (CMV >, EBV >, HBV >, HIV >, etc.); Alzheimer Peptides; Cancer Research (Melan A (MART1) >, MAGE >, HER-2 >, p53 >); Cell Penetrating Peptides (Arg9 >, CyLoP-1 >, HIV-1 TAT >) and others as PADRE >; PRAME (100-108) HLA-A*02:01 >; MBP (1-11) human >; CD20 (188-196) HLA-A*02:01 >.

CMRL-1066 without L-Glutamine, without Phenol red, with 2.2g/L NaHCO3, sterile filtered. CMRL is a nucleoside and vitamin-rich medium. In the past it was developed to clone monkey-kidney cells and as long time culture medium for L-cells. It is sutiable for many types of human and monkey cells and also for other mammalian cells, especially by using horse and foetal bovine serum. If the composition of the medium does not fit: You can freely choose the composition of our special media. Just let us know your needs. Ask for a quote. Genaxxon offers also other products as our broad range of peptides from stock > for your research. For example: Virus Epitope Peptides (CMV >, EBV >, HBV >, HIV >, etc.); Alzheimer Peptides; Cancer Research (Melan A (MART1) >, MAGE >, HER-2 >, p53 >); Cell Penetrating Peptides (Arg9 >, CyLoP-1 >, HIV-1 TAT >) and others as PADRE >; PRAME (100-108) HLA-A*02:01 >; MBP (1-11) human >; CD20 (188-196) HLA-A*02:01 >.

Colcemid inhibits the formation of mitotic spindles. It is used to increase the percentage of metaphase cells for chromosome analysis. Colcemid depolymerizes microtubules; blocks mitosis at metaphase. Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase. You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.

Clostridium histolyticum collagenase is an enzyme mixture of collagenase, clostripain and tryptic and proteolytic activities. Collagenase type I shows a balanced activity of collagenase, clostripain as well as tryptic and proteolytic activities. Type I Collagenase is recommended for cell preparation from epithelial, liver, lung tissue, tissue of the suprarenal gland and adipose tissue. The specific activity is >90 Mandl-Units per mg dry substance. Collagenase is produced by two separate and distinct genes in Clostridium histolyticum. Both genes have been cloned and sequenced (Yoshihara 1994). The colG gene codes for type I collagenase, a 936 amino acid protein, while the colH gene codes for type II collagenase, a 1021 amino acid protein. Both genes share 72% identity, the proteins only 43%. Both gene products can be present as two or more isoforms differing in molecular weight. Therefore collagenase mixtures can contain six to eight different proteins in a molecular weight range from 68 to 130 kDa. Substrate specificity studies have demonstrated that the colG gene prefers natural substrates such as intact collagen, compared to the colH gene product which preferentially digests short synthetic substrates (FALGPA) (Eckhard et al. 2009 and Matsushita 1999). General description:The treatment of tissue with collagenase causes the careful, selective degradation of the intercellular matrix, and does not affect the growth of the cells. The collagenase offered by Genaxxon bioscience is a mixture of different proteolytically active enzymes and requires calcium ions for both the catalytic activity and the binding to the collagen molecule. In contrast to vertebrate collagenase, Clostridium histolyticum collagenase digests native triple helix collagen into small peptides, which is the major use of collagenases in cell culture application. For optimal results, a well balanced mixture of proteolytic enzymes is necessary. Four different collagenases, Collagenase Type I >, Collagenase Type II >, Collagenase Type III > and Collagenase Type IV > are available for this. Type IV is usually used together with other enzymes, such as trypsin >, elastase or hyaluronidase >. Trypsin or Trypsin/EDTA > conventionally used in the cell culture degrades the matrix only slowly and can cause irreversible damage to the released cells. Therefore, we recommend Accutase > instead of trypsin or trypsin/EDTA. Clostridium collagenases belongs to the metalloproteases, a large family of proteases that shares a zinc-containing motif at the center of the active site (Gonzales and Robert-Baudouy 1996). The enzyme is reversibly inactivated at high pH values and irreversibly inactivated at low pH values. Inhibitors of collagenase include cysteine, EDTA, o-phenanthroline, 8-hydroxyquinoline-5-sulfonate, bipyridyl, 2,3-dimercaptopropanol or Hg2+, Pb2+, Cd2+, Cu2+. Collagenase is NOT inhibited by diisopropylphosphorofluoridate (DFP) or serum. Lyphilised Collagenase should be stored at +2°C to +8°C and remain stable without loss of activity for at least three years. Enzyme should be protected from moisture. Dissolved Collagenase can be aliquoted and stored at -20°C for one year. Conversion rates of collagenase activity units: 1 PZ U/mg ~ 3.9 FALGPA U/mg - 1 PZ U/mg ~ 1000 Mandl or CDU U/mg - 1 PZ U/mg ~ 10 HP U/mg (PZ-units according to Wünsch).

Clostridium histolyticum collagenase is an enzyme mixture of collagenase, clostripain and tryptic and proteolytic activities. Type II Collagenase is recommended for the preparation of cells from liver, bone, thyroid gland, heart and salivary gland and has a specific activity of >180 Mandl units per mg of dry matter. Collagenase is produced by two separate and distinct genes in Clostridium histolyticum. Both genes have been cloned and sequenced (Yoshihara 1994). The colG gene codes for type I collagenase, a 936 amino acid protein, while the colH gene codes for type II collagenase, a 1021 amino acid protein. Both genes share 72% identity, the proteins only 43%. Both gene products can be present as two or more isoforms differing in molecular weight. Therefore collagenase mixtures can contain six to eight different proteins in a molecular weight range from 68 to 130 kDa. Substrate specificity studies have demonstrated that the colG gene prefers natural substrates such as intact collagen, compared to the colH gene product which preferentially digests short synthetic substrates (FALGPA) (Eckhard et al. 2009 and Matsushita 1999). General description:The treatment of tissue with collagenase causes the careful, selective degradation of the intercellular matrix, and does not affect the growth of the cells. The collagenase offered by Genaxxon bioscience is a mixture of different proteolytically active enzymes and requires calcium ions for both the catalytic activity and the binding to the collagen molecule. In contrast to vertebrate collagenase, Clostridium histolyticum collagenase digests native triple helix collagen into small peptides, which is the major use of collagenases in cell culture application. For optimal results, a well balanced mixture of proteolytic enzymes is necessary. Four different collagenases, Collagenase Type I >, Collagenase Type II >, Collagenase Type III > and Collagenase Type IV > are available for this. Type IV is usually used together with other enzymes, such as trypsin >, elastase or hyaluronidase >. Trypsin or Trypsin/EDTA > conventionally used in the cell culture degrades the matrix only slowly and can cause irreversible damage to the released cells. Therefore, we recommend Accutase > instead of trypsin or trypsin/EDTA. Clostridium collagenases belongs to the metalloproteases, a large family of proteases that shares a zinc-containing motif at the center of the active site (Gonzales and Robert-Baudouy 1996). The enzyme is reversibly inactivated at high pH values and irreversibly inactivated at low pH values. Inhibitors of collagenase include cysteine, EDTA, o-phenanthroline, 8-hydroxyquinoline-5-sulfonate, bipyridyl, 2,3-dimercaptopropanol or Hg2+, Pb2+, Cd2+, Cu2+. Collagenase is NOT inhibited by diisopropylphosphorofluoridate (DFP) or serum. Lyphilised Collagenase should be stored at +2°C to +8°C and remain stable without loss of activity for at least three years. Enzyme should be protected from moisture. Dissolved Collagenase can be aliquoted and stored at -20°C for one year. Conversion rates of collagenase activity units: 1 PZ U/mg ~ 3.9 FALGPA U/mg - 1 PZ U/mg ~ 1000 Mandl or CDU U/mg - 1 PZ U/mg ~ 10 HP U/mg (PZ-units according to Wünsch).

Clostridium histolyticum collagenase is an enzyme mixture of collagenase, clostripain and tryptic and proteolytic activities. Collagenase type III shows normal Collagenase, but very low proteolytic activity. Type III Collagenase is recommended for preparation of cells from mamary gland and fetal cells and has a specific activity of 100 to 250 Mandl units per mg of dry matter. Collagenase is produced by two separate and distinct genes in Clostridium histolyticum. Both genes have been cloned and sequenced (Yoshihara 1994). The colG gene codes for type I collagenase, a 936 amino acid protein, while the colH gene codes for type II collagenase, a 1021 amino acid protein. Both genes share 72% identity, the proteins only 43%. Both gene products can be present as two or more isoforms differing in molecular weight. Therefore collagenase mixtures can contain six to eight different proteins in a molecular weight range from 68 to 130 kDa. Substrate specificity studies have demonstrated that the colG gene prefers natural substrates such as intact collagen, compared to the colH gene product which preferentially digests short synthetic substrates (FALGPA) (Eckhard et al. 2009 and Matsushita 1999). General description:The treatment of tissue with collagenase causes the careful, selective degradation of the intercellular matrix, and does not affect the growth of the cells. The collagenase offered by Genaxxon bioscience is a mixture of different proteolytically active enzymes and requires calcium ions for both the catalytic activity and the binding to the collagen molecule. In contrast to vertebrate collagenase, Clostridium histolyticum collagenase digests native triple helix collagen into small peptides, which is the major use of collagenases in cell culture application. For optimal results, a well balanced mixture of proteolytic enzymes is necessary. Four different collagenases, Collagenase Type I >, Collagenase Type II >, Collagenase Type III > and Collagenase Type IV > are available for this. Type IV is usually used together with other enzymes, such as trypsin >, elastase or hyaluronidase >. Trypsin or Trypsin/EDTA > conventionally used in the cell culture degrades the matrix only slowly and can cause irreversible damage to the released cells. Therefore, we recommend Accutase > instead of trypsin or trypsin/EDTA. Clostridium collagenases belongs to the metalloproteases, a large family of proteases that shares a zinc-containing motif at the center of the active site (Gonzales and Robert-Baudouy 1996). The enzyme is reversibly inactivated at high pH values and irreversibly inactivated at low pH values. Inhibitors of collagenase include cysteine, EDTA, o-phenanthroline, 8-hydroxyquinoline-5-sulfonate, bipyridyl, 2,3-dimercaptopropanol or Hg2+, Pb2+, Cd2+, Cu2+. Collagenase is NOT inhibited by diisopropylphosphorofluoridate (DFP) or serum. Lyphilised Collagenase should be stored at +2°C to +8°C and remain stable without loss of activity for at least three years. Enzyme should be protected from moisture. Dissolved Collagenase can be aliquoted and stored at -20°C for one year. Conversion rates of collagenase activity units: 1 PZ U/mg ~ 3.9 FALGPA U/mg - 1 PZ U/mg ~ 1000 Mandl or CDU U/mg - 1 PZ U/mg ~ 10 HP U/mg (PZ-units according to Wünsch).

Clostridium histolyticum collagenase is an enzyme mixture of collagenase, clostripain and tryptic and proteolytic activities. Collagenase type IV has low tryptic, high collagenase and normal clostripain activity. Type I Collagenase is recommended for cell preparation from epithelial, liver, lung tissue, tissue of the suprarenal gland and adipose tissue. The specific activity of >900 Mandl units per mg of dry matter. Collagenase is produced by two separate and distinct genes in Clostridium histolyticum. Both genes have been cloned and sequenced (Yoshihara 1994). The colG gene codes for type I collagenase, a 936 amino acid protein, while the colH gene codes for type II collagenase, a 1021 amino acid protein. Both genes share 72% identity, the proteins only 43%. Both gene products can be present as two or more isoforms differing in molecular weight. Therefore collagenase mixtures can contain six to eight different proteins in a molecular weight range from 68 to 130 kDa. Substrate specificity studies have demonstrated that the colG gene prefers natural substrates such as intact collagen, compared to the colH gene product which preferentially digests short synthetic substrates (FALGPA) (Eckhard et al. 2009 and Matsushita 1999). General description:The treatment of tissue with collagenase causes the careful, selective degradation of the intercellular matrix, and does not affect the growth of the cells. The collagenase offered by Genaxxon bioscience is a mixture of different proteolytically active enzymes and requires calcium ions for both the catalytic activity and the binding to the collagen molecule. In contrast to vertebrate collagenase, Clostridium histolyticum collagenase digests native triple helix collagen into small peptides, which is the major use of collagenases in cell culture application. For optimal results, a well balanced mixture of proteolytic enzymes is necessary. Four different collagenases, Collagenase Type I >, Collagenase Type II >, Collagenase Type III > and Collagenase Type IV > are available for this. Type IV is usually used together with other enzymes, such as trypsin >, elastase or hyaluronidase >. Trypsin or Trypsin/EDTA > conventionally used in the cell culture degrades the matrix only slowly and can cause irreversible damage to the released cells. Therefore, we recommend Accutase > instead of trypsin or trypsin/EDTA. Clostridium collagenases belongs to the metalloproteases, a large family of proteases that shares a zinc-containing motif at the center of the active site (Gonzales and Robert-Baudouy 1996). The enzyme is reversibly inactivated at high pH values and irreversibly inactivated at low pH values. Inhibitors of collagenase include cysteine, EDTA, o-phenanthroline, 8-hydroxyquinoline-5-sulfonate, bipyridyl, 2,3-dimercaptopropanol or Hg2+, Pb2+, Cd2+, Cu2+. Collagenase is NOT inhibited by diisopropylphosphorofluoridate (DFP) or serum. Lyphilised Collagenase should be stored at +2°C to +8°C and remain stable without loss of activity for at least three years. Enzyme should be protected from moisture. Dissolved Collagenase can be aliquoted and stored at -20°C for one year. Conversion rates of collagenase activity units: 1 PZ U/mg ~ 3.9 FALGPA U/mg - 1 PZ U/mg ~ 1000 Mandl or CDU U/mg - 1 PZ U/mg ~ 10 HP U/mg (PZ-units according to Wünsch).

Cytosin, 4-Amino-2-hydroxypyrimidine. Cytosine is one of the four main bases found in DNA and RNA, along with adenine, guanine, and thymine (uracil in RNA). It is a pyrimidine derivative, with a heterocyclic aromatic ring and two substituents attached (an amine group at position 4 and a keto group at position 2). The nucleoside of cytosine is cytidine. In Watson-Crick base pairing, it forms three hydrogen bonds with guanine.

Dulbecco's Modified Eagle Medium (DMEM), with 4.5g/L glucose, no glutamine, no lysine, no arginine is a basal cell culture medium for use during SILAC™ protein labeling with stable isotopic labeled lysine and/or arginine. Special preparation. Minimum order size: 20 x 500mL. DMEM for SILAC™ has been specifically formulated for use with stable isotope labelling with amino acids in cell culture (SILAC) for the analysis of protein expression by mass spectrometry (MS).SILAC™ is a powerful method for identifying and quantifying even differential changes in complex protein samples. The approach involves in-vivo integration of stable 13C- or 15N-labelled amino acids into proteins, followed by mass spectrometry (MS) for rapid and comprehensive protein identification, characterisation and quantification. DMEM, intrinsically developed for the cultivation of murine embryonic cells, is tailor-made for the cultivation of a broad range of cells, especially if the media is supplemented with FCS. DMEM is an Eagle Medium modification with the four-fold content of amino acids and vitamin. While DMEM with 1.0g/L Glucose is the standard media, DMEM with 4.5g/L Glucose is for cells which have a high energy demand. is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Genaxxon offers a variety of DMEM modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com. Genaxxon offers also other products as our broad range of peptides from stock > for your research. For example: Virus Epitope Peptides (CMV >, EBV >, HBV >, HIV >, etc.); Alzheimer Peptides; Cancer Research (Melan A (MART1) >, MAGE >, HER-2 >, p53 >); Cell Penetrating Peptides (Arg9 >, CyLoP-1 >, HIV-1 TAT >) and others as PADRE >; PRAME (100-108) HLA-A*02:01 >; MBP (1-11) human >; CD20 (188-196) HLA-A*02:01 >.

Dulbecco's Modified Eagle Medium (DMEM), 4.5g/L glucose, no L-glutamine, no L-arginine, with Sodium pyruvate.Special preparation. Minimum order size: 20 x 500mL. DMEM, intrinsically developed for the cultivation of murine embryonic cells, is tailor-made for the cultivation of a broad range of cells, especially if the media is supplemented with FCS. DMEM is an Eagle Medium modification with the four-fold content of amino acids and vitamin. While DMEM with 1.0g/L Glucose is the standard media, DMEM with 4.5g/L Glucose is for cells which have a high energy demand. is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Genaxxon offers a variety of DMEM modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com. Genaxxon offers also other products as our broad range of peptides from stock > for your research. For example: Virus Epitope Peptides (CMV >, EBV >, HBV >, HIV >, etc.); Alzheimer Peptides; Cancer Research (Melan A (MART1) >, MAGE >, HER-2 >, p53 >); Cell Penetrating Peptides (Arg9 >, CyLoP-1 >, HIV-1 TAT >) and others as PADRE >; PRAME (100-108) HLA-A*02:01 >; MBP (1-11) human >; CD20 (188-196) HLA-A*02:01 >.

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. DMEM contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. It was originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels (4.5g/L) for cells with a high energy demand. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). As DMEM uses a sodium bicarbonate buffer system (3.7g/L), a 5–10% CO2 environment is necessary to maintain physiological pH. Genaxxon offers a variety of DMEM modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com. Genaxxon offers also other products as our broad range of peptides from stock > for your research. For example: Virus Epitope Peptides (CMV >, EBV >, HBV >, HIV >, etc.); Alzheimer Peptides; Cancer Research (Melan A (MART1) >, MAGE >, HER-2 >, p53 >); Cell Penetrating Peptides (Arg9 >, CyLoP-1 >, HIV-1 TAT >) and others as PADRE >; PRAME (100-108) HLA-A*02:01 >; MBP (1-11) human >; CD20 (188-196) HLA-A*02:01 >.

Dulbecco's Modified Eagle Medium (DMEM), 1.0g/L glucose, no L-glutamine, no amino acids. Special preparation. Minimal order size: depending on stock at least 2 x 500mL. DMEM, intrinsically developed for the cultivation of murine embryonic cells, is tailor-made for the cultivation of a broad range of cells, especially if the media is supplemented with FCS. DMEM is an Eagle Medium modification with the four-fold content of amino acids and vitamin. While DMEM with 1.0g/L Glucose is the standard media, DMEM with 4.5g/L Glucose is for cells which have a high energy demand. is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. DMEM contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. It was originally formulated with low glucose (1.0g/L) and Sodium pyruvate, but is often used with higher glucose levels (4.5g/L). DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). As DMEM uses a sodium bicarbonate buffer system (3.7g/L), a 5–10% CO2 environment is necessary to maintain physiological pH. Genaxxon offers a variety of DMEM modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com. Genaxxon offers also other products as our broad range of peptides from stock > for your research. For example: Virus Epitope Peptides (CMV >, EBV >, HBV >, HIV >, etc.); Alzheimer Peptides; Cancer Research (Melan A (MART1) >, MAGE >, HER-2 >, p53 >); Cell Penetrating Peptides (Arg9 >, CyLoP-1 >, HIV-1 TAT >) and others as PADRE >; PRAME (100-108) HLA-A*02:01 >; MBP (1-11) human >; CD20 (188-196) HLA-A*02:01 >.

Dulbecco's Modified Eagle Medium (DMEM), 4.5g/L glucose, no L-glutamine, no amino acids. Special preparation. Minimal order size: 20 x 500mL. DMEM, intrinsically developed for the cultivation of murine embryonic cells, is tailor-made for the cultivation of a broad range of cells, especially if the media is supplemented with FCS. DMEM is an Eagle Medium modification with the four-fold content of amino acids and vitamin. While DMEM with 1.0g/L Glucose is the standard media, DMEM with 4.5g/L Glucose is for cells which have a high energy demand. is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. DMEM contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. It was originally formulated with low glucose (1.0g/L) and Sodium pyruvate, but is often used with higher glucose levels (4.5g/L). DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). As DMEM uses a sodium bicarbonate buffer system (3.7g/L), a 5–10% CO2 environment is necessary to maintain physiological pH. Genaxxon offers a variety of DMEM modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com. Genaxxon offers also other products as our broad range of Antibiotcs > - Amphotericin B > - Antibiotic Antimycotic Solution > - Doxycycline > - Geneticin disulfate (G418) > - Gentamycin sulfate > - Hygromycin B > - Kanamycin > - Penicillin/Streptomycin >.

Dulbecco's Modified Eagle Medium (DMEM), 4.5g/L glucose, no glutamine, no lysine, no arginine, no methionine is a basal cell culture medium for use during SILAC™ protein labeling with stable isotopic labeled lysine and/or arginine. Special preparation. Minimum order size: 20 x 500mL. DMEM, intrinsically developed for the cultivation of murine embryonic cells, is tailor-made for the cultivation of a broad range of cells, especially if the media is supplemented with FCS. DMEM is an Eagle Medium modification with the four-fold content of amino acids and vitamin. While DMEM with 1.0g/L Glucose is the standard media, DMEM with 4.5g/L Glucose is for cells which have a high energy demand. is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. DMEM contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. It was originally formulated with low glucose (1.0g/L) and Sodium pyruvate, but is often used with higher glucose levels (4.5g/L). DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). As DMEM uses a sodium bicarbonate buffer system (3.7g/L), a 5–10% CO2 environment is necessary to maintain physiological pH. Genaxxon offers a variety of DMEM modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com. Genaxxon offers also other products as our broad range of Antibiotcs > - Amphotericin B > - Antibiotic Antimycotic Solution > - Doxycycline > - Geneticin disulfate (G418) > - Gentamycin sulfate > - Hygromycin B > - Kanamycin > - Penicillin/Streptomycin >.

Dulbecco's Modified Eagle Medium (DMEM), no glucose, no glutamine, no lysine, no arginine is a basal cell culture medium for use during SILAC™ protein labeling with stable isotopic labeled lysine and/or arginine. Special preparation. Minimum order size: 20 x 500mL. DMEM, intrinsically developed for the cultivation of murine embryonic cells, is tailor-made for the cultivation of a broad range of cells, especially if the media is supplemented with FCS. DMEM is an Eagle Medium modification with the four-fold content of amino acids and vitamin. While DMEM with 1.0g/L Glucose is the standard media, DMEM with 4.5g/L Glucose is for cells which have a high energy demand. is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Genaxxon offers a variety of DMEM modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com. Genaxxon offers also other products as our broad range of peptides from stock > for your research. For example: Virus Epitope Peptides (CMV >, EBV >, HBV >, HIV >, etc.); Alzheimer Peptides; Cancer Research (Melan A (MART1) >, MAGE >, HER-2 >, p53 >); Cell Penetrating Peptides (Arg9 >, CyLoP-1 >, HIV-1 TAT >) and others as PADRE >; PRAME (100-108) HLA-A*02:01 >; MBP (1-11) human >; CD20 (188-196) HLA-A*02:01 >.

Dulbecco's Modified Eagle Medium (DMEM), w/o glucose, no L-glutamine, no L-cysteine, no L-methionine, no Sodium pyruvate.Special preparation. Minimum order size: 20 x 500mL. DMEM, intrinsically developed for the cultivation of murine embryonic cells, is tailor-made for the cultivation of a broad range of cells, especially if the media is supplemented with FCS. DMEM is an Eagle Medium modification with the four-fold content of amino acids and vitamin. While DMEM with 1.0g/L Glucose is the standard media, DMEM with 4.5g/L Glucose is for cells which have a high energy demand. is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. DMEM contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. It was originally formulated with low glucose (1.0g/L) and Sodium pyruvate, but is often used with higher glucose levels (4.5g/L). DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). As DMEM uses a sodium bicarbonate buffer system (3.7g/L), a 5–10% CO2 environment is necessary to maintain physiological pH. Genaxxon offers a variety of DMEM modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com. Genaxxon offers also other products as our broad range of peptides from stock > for your research. For example: Virus Epitope Peptides (CMV >, EBV >, HBV >, HIV >, etc.); Alzheimer Peptides; Cancer Research (Melan A (MART1) >, MAGE >, HER-2 >, p53 >); Cell Penetrating Peptides (Arg9 >, CyLoP-1 >, HIV-1 TAT >) and others as PADRE >; PRAME (100-108) HLA-A*02:01 >; MBP (1-11) human >; CD20 (188-196) HLA-A*02:01 >.

Dulbecco's Modified Eagle Medium (DMEM), 4.5g/L glucose, w/o L-Glutamine, w/o non-essentail amino acids, w/o Sodium pyruvate, w/o NaHCO3.Special preparation. Minimum order size: 20 x 500mL. DMEM, intrinsically developed for the cultivation of murine embryonic cells, is tailor-made for the cultivation of a broad range of cells, especially if the media is supplemented with FCS. DMEM is an Eagle Medium modification with the four-fold content of amino acids and vitamin. While DMEM with 1.0g/L Glucose is the standard media, DMEM with 4.5g/L Glucose is for cells which have a high energy demand. is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Genaxxon offers a variety of DMEM modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com. Genaxxon offers also other products as our broad range of peptides from stock > for your research. For example: Virus Epitope Peptides (CMV >, EBV >, HBV >, HIV >, etc.); Alzheimer Peptides; Cancer Research (Melan A (MART1) >, MAGE >, HER-2 >, p53 >); Cell Penetrating Peptides (Arg9 >, CyLoP-1 >, HIV-1 TAT >) and others as PADRE >; PRAME (100-108) HLA-A*02:01 >; MBP (1-11) human >; CD20 (188-196) HLA-A*02:01 >.

Dulbecco's Modified Eagle Medium (DMEM), 4.5g/L glucose, no L-cysteine, no L-methionine, no L-glutamine.Special preparation. Minimum order size: 20 x 500mL. DMEM, intrinsically developed for the cultivation of murine embryonic cells, is tailor-made for the cultivation of a broad range of cells, especially if the media is supplemented with FCS. DMEM is an Eagle Medium modification with the four-fold content of amino acids and vitamin. While DMEM with 1.0g/L Glucose is the standard media, DMEM with 4.5g/L Glucose is for cells which have a high energy demand. is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Genaxxon offers a variety of DMEM modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com. Genaxxon offers also other products as our broad range of peptides from stock > for your research. For example: Virus Epitope Peptides (CMV >, EBV >, HBV >, HIV >, etc.); Alzheimer Peptides; Cancer Research (Melan A (MART1) >, MAGE >, HER-2 >, p53 >); Cell Penetrating Peptides (Arg9 >, CyLoP-1 >, HIV-1 TAT >) and others as PADRE >; PRAME (100-108) HLA-A*02:01 >; MBP (1-11) human >; CD20 (188-196) HLA-A*02:01 >.

Dulbecco's Modified Eagle Medium (DMEM), 1.0g/L glucose, no L-glutamine, no L-isoleucine.Special preparation. Minimum order size: 20 x 500mL. DMEM, intrinsically developed for the cultivation of murine embryonic cells, is tailor-made for the cultivation of a broad range of cells, especially if the media is supplemented with FCS. DMEM is an Eagle Medium modification with the four-fold content of amino acids and vitamin. While DMEM with 1.0g/L Glucose is the standard media, DMEM with 4.5g/L Glucose is for cells which have a high energy demand. is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. DMEM contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. It was originally formulated with low glucose (1.0g/L) and Sodium pyruvate, but is often used with higher glucose levels (4.5g/L). DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). As DMEM uses a sodium bicarbonate buffer system (3.7g/L), a 5–10% CO2 environment is necessary to maintain physiological pH. Genaxxon offers a variety of DMEM modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com. Genaxxon offers also other products as our broad range of peptides from stock > for your research. For example: Virus Epitope Peptides (CMV >, EBV >, HBV >, HIV >, etc.); Alzheimer Peptides; Cancer Research (Melan A (MART1) >, MAGE >, HER-2 >, p53 >); Cell Penetrating Peptides (Arg9 >, CyLoP-1 >, HIV-1 TAT >) and others as PADRE >; PRAME (100-108) HLA-A*02:01 >; MBP (1-11) human >; CD20 (188-196) HLA-A*02:01 >.