DNA Loading buffer I Fluoro (6x)
Order number: M3323.0001
Shipping: Shipment: not cooled. Stored at -20°C. For laboratory usage only! Ready to ship today,
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Loading buffer I Fluoro is a fluorescent reagent that produces instant visualization of DNA bands upon blue light or UV illumination of agarose gels. Supplied in 6X DNA Loading Buffer, Loading buffer I Fluoro is used to prepare DNA markers or samples for loading on agarose or polyacrylamide gels.
Loading buffer I Fluoro is the sensitive staining reagent available for detecting the double-stranded DNA (dsDNA). It contains three tracking dyes (bromophenol blue, xylene cyanol FF, and orange G) for visually tracking the DNA migration during the electrophoresis plus an additional fluorescence dye for detection of DNA bands without an additional staining of the gel. It is an ideal alternative to Ethidium Bromide (EtBr).
Approximate fluorescence excitation / emission: 300, 495 / 537 nm, bound to nucleic acid.
Picture 1: Comparison of UV- and blue light excitation Picture 2: Effect of EtBr plus UV on cloning efficiency.
◈ Sensitivity – High sensitivity at least as Ethidium Bromide. ◈ Convenience – Ready to Use. Same application procedures as the 6X Loading Dye. ◈ Time efficiency – No staining requirement. No de-staining requirement. Low background value. Imaging directly during or after electrophoresis possible. ◈ Compatibility – Use of blue light or UV for detection. ◈ Economic – No expenses required for waste management.
Technical Data:
Specifications:
6-time loading buffer for agarose gels
contains: Glycerin, EDTA, Bromophenol blue, Xyclene cyanol, Orange G plus a fluorescence dye
application:
for application of DNA onto Agarose and Polyacrylamide gelsSource
syntheticSicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02Dokumente - Protokolle - Downloads
Here you will find information and further literature on DNA Loading buffer I Fluoro (6x). For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
