Realtime PCR

Genaxxon´s Green and Probe PCR master mixes > for Real Time Quantitative PCR are especially designed and optimized for block cycler, e.g. Roche (LightCycler® 480), Qiagen (Rotor-Gene®), LifeTechnologies (StepOnePlus®) or Applied Biosystems (Mx3005P®) instruments.

Real time PCR, also known as quantitative or qPCR, is an effective tool to make the in-vitro amplification of specific DNA fragments visible without using an agarose gel. As the term implies, the amplification process can be analyzed while PCR is still proceeding (real time). By using real time PCR instruments amplification and fluorescence detection can be combined in a single step. An additional, time-consuming and not easily quantifiable gel electrophoresis is not required.

Genaxxon Green Mastermixes (High ROX®, Low ROX® and without ROX®) contain a green fluorescent intercalating dye >. The mastermixes are optimized for real time PCR in block systems. They contain all the components in order to perform a quantitative PCR, with the exception of the primers and the template DNA. The Probe Mastermixes are without fluorescent dye respectively.

Price comparison Genaxxon qPCR master mixes - selected qPCR master mixes of leading suppliers >

Online form for your qPCR master mix test sample >

Genaxxon´s Green and Probe PCR master mixes > for Real Time Quantitative PCR are especially designed and optimized for block cycler, e.g. Roche (LightCycler ®  480), Qiagen (Rotor-Gene... read more »
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Realtime PCR

Genaxxon´s Green and Probe PCR master mixes > for Real Time Quantitative PCR are especially designed and optimized for block cycler, e.g. Roche (LightCycler® 480), Qiagen (Rotor-Gene®), LifeTechnologies (StepOnePlus®) or Applied Biosystems (Mx3005P®) instruments.

Real time PCR, also known as quantitative or qPCR, is an effective tool to make the in-vitro amplification of specific DNA fragments visible without using an agarose gel. As the term implies, the amplification process can be analyzed while PCR is still proceeding (real time). By using real time PCR instruments amplification and fluorescence detection can be combined in a single step. An additional, time-consuming and not easily quantifiable gel electrophoresis is not required.

Genaxxon Green Mastermixes (High ROX®, Low ROX® and without ROX®) contain a green fluorescent intercalating dye >. The mastermixes are optimized for real time PCR in block systems. They contain all the components in order to perform a quantitative PCR, with the exception of the primers and the template DNA. The Probe Mastermixes are without fluorescent dye respectively.

Price comparison Genaxxon qPCR master mixes - selected qPCR master mixes of leading suppliers >

Online form for your qPCR master mix test sample >

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PHOENIXDX® 2019-NCOV
PHOENIXDX® 2019-NCOV
PHOENIXDX® 2019-NCOV is a real-time RT-PCR-based detection system for the 2019 Wuhan coronavirus (2019-nCoV). The kit comes as 20-time RT-PCR master mix. PHOENIXDX® 2019-NCOV is a real-time RT-PCR-based detection system for the 2019...
€475.00 *
1copy™ COVID-19 qPCR Kit
1copy™ COVID-19 qPCR Kit
1copy™ COVID-19 qPCR Kit provides the fast and accurate testing solution for COVID-19, specifically targeting the E gene for beta coronavirus and the RdRp gene for COVID-19 in sputum, nasopharyngeal swab and oropharyngeal swab. The...
€2,550.00 *
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realtime PCR master mix without ROX from Genaxxon
GreenMasterMix (2X) No ROX for qPCR
GreenMasterMix without ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains...
From €20.00 *
realtime PCR master mix without ROX from Genaxxon
ProbeMasterMix No ROX for qPCR
ProbeMasterMix without ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains...
From €20.00 *
ProbeMasterMix (2X) Low ROX for qPCR
ProbeMasterMix (2X) Low ROX for qPCR
ProbeMasterMix low ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X master mix is ready-to-use and contains optimised...
From €20.00 *
GreenMasterMix (2X) Low ROX for qPCR
GreenMasterMix (2X) Low ROX for qPCR
GreenMastermix Low ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains optimised...
From €20.00 *
Multiplex HS MasterMix (2X)
Multiplex HS MasterMix (2X)
Multiplex PCR is a method that enables amplification of two or more amplicons simultaneously in a single reaction tube/reaction. It is widely used in genotyping and different areas of DNA testing in research, forensic and diagnostic...
From €165.00 *
Hotstart PCR Mastermixes from Genaxxon
SuperHot Taq PCR Kit with dNTP mix
SuperHot Taq DNA polymerase is a chemicaly modified form of thermostable DNA polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room...
From €90.00 *
Hotstart Taq from Genaxxon
SuperHot Taq DNA Polymerase - chemically...
SuperHot Taq DNA Polymerase is a superior DNA Polymerase for Real Time PCR, Hot-Start PCR, low-copy number PCR, or PCR of difficult templates. The enzyme is developed to enhance the specificity, sensitivity and yield of DNA...
From €63.00 * €90.00 *
Hint!
GreenMasterMix (2X) High ROX for qPCR
GreenMasterMix (2X) High ROX for qPCR
GreenMastermix High ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains optimised...
From €20.00 *
LyoCake - lyophilized qPCR master mix
qPCR Green LyoMix HRM - No ROX - lyophilized...
The qPCR Green LyoMix HRM Master Mix is a lyophilized, temperature stable, premixed and pre-portioned Hotstart master mix with all necessary components optimized for e.g. highly specific qPCR or post-PCR high-resolution melt analysis...
From €30.25 *
LyoBeads in PCR tube strip
LyoBalls in 0.1mL tube strips or 96-well PCR...
While using our LyoBalls there is no need for cooling anymore! The balls and all ingredients are unopened stable at ambient temperature for at least 24 months! Test sample (1 strip of 8 tubes - (low profile 0.1mL)) available! No shipping...
From €11.00 *
LyoBeads in PCR tube strip
LyoBalls in 0.2mL tube strips or 96-well PCR...
While using our LyoBalls there is no need for cooling anymore! The balls and all ingredients are unopened stable at ambient temperature for at least 24 months! Test sample (1 strip of 8 tubes - standard profile (0.2mL)) available! No...
From €11.00 *
real time PCR master mix with high ROX from Genaxxon
ProbeMasterMix (2X) High ROX for qPCR
ProbeMastermix High ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains optimised...
From €20.00 *
LyoCake - lyophilized qPCR master mix
LyoMix - Lyophilized qPCR probe master mix powder
Lyophilized (freeze-dried) temperature stable and pre-mixed Hotstart master mix with all necessary components for probe realtime PCR (optimized buffer, dNTPs, Hotstart Taq) for difficult to amplify templates, qPCR and Multiplex...
From €30.25 *
LyoPlex Multiplex PCR master mix - lyophilized
LyoPlex Multiplex PCR master mix - lyophilized
LyoPlex Multiplex PCR master mix for fast and reliable quantification of up to 5 cDNA or gDNA targets in a single tube by multiplex, real-time PCR. This specially optimized and lyophilized master mix promotes fast multiplex real-time...
From €167.48 *
Tempase Hot Start Polymerase
TEMPase Hot Start DNA Polymerase
About TEMPase Hot Start DNA Polymerase TEMPase Hot Start DNA Polymerase has been designed to avoid spurious amplification and to diminish the formation of non specific priming events during your set up. TEMPase Hot Start DNA Polymerase...
From €90.00 *
Hotstart Taq from Genaxxon
HotStart Taq DNA Polymerase with Antibody
Taq DNA polymerase with hot start monoclonal antibody: The antibody inhibits the polymerase activity prior to the first denaturation step during the Hot Start PCR and is inactivated by temperatures above 75°C. The HotStart Taq DNA...
From €70.00 *
Tempase Hot Start Polymerase with Ammonium Buffer
TEMPase Hot Start DNA Polymerase
About TEMPase Hot Start DNA Polymerase TEMPase Hot Start DNA Polymerase has been designed to avoid spurious amplification and to diminish the formation of non specific priming events during your set up. TEMPase Hot Start DNA Polymerase...
From €90.00 *
RealQ Plus 2x Mastermix für realtime PCR
RealQ Plus 2x Master Mix for Probe - Low ROX
About RealQ PCR Master Mix Quantitative polymerase chain reaction (qPCR) is the preferred method for gene analysis. RealQ PCR 2 x Master Mix is well suited for gene expression, genotyping, SNP analysis, pathogen detection, drug target...
From €241.50 *
RealQ Plus 2x Mastermix für realtime PCR
RealQ Plus 2x Master Mix for Probe - without ROX
About RealQ PCR Master Mix Quantitative polymerase chain reaction (qPCR) is the preferred method for gene analysis. RealQ PCR 2 x Master Mix is well suited for gene expression, genotyping, SNP analysis, pathogen detection, drug target...
From €241.50 *
RealQ Plus 2x Mastermix für realtime PCR
RealQ Plus 2x Master Mix for Probe - High ROX
About RealQ PCR Master Mix Quantitative polymerase chain reaction (qPCR) is the preferred method for gene analysis. RealQ PCR 2 x Master Mix is well suited for gene expression, genotyping, SNP analysis, pathogen detection, drug target...
From €241.50 *
RealQ Plus 2x Mastermix für realtime PCR
RealQ Plus 2x Master Mix Green - High ROX
Introduction: Quantitative PCR is an important tool for SNP and gene expression analysis. Two general fluorescent chemistries exist for quantitative detection of gene transcripts: probes (e.g. TaqMan®, Scorpions™ Probes, molecular...
From €241.50 *
RealQ Plus 2x Mastermix für realtime PCR
RealQ Plus 2x Master Mix Green - Low ROX
Introduction: Quantitative PCR is an important tool for SNP and gene expression analysis. Two general fluorescent chemistries exist for quantitative detection of gene transcripts: probes (e.g. TaqMan®, Scorpions™ Probes, molecular...
From €241.50 *
RealQ Plus 2x Mastermix für realtime PCR
RealQ Plus 2x Master Mix Green - Without ROX
Introduction: Quantitative PCR is an important tool for SNP and gene expression analysis. Two general fluorescent chemistries exist for quantitative detection of gene transcripts: probes (e.g. TaqMan®, Scorpions™ Probes, molecular...
From €241.50 *