Products for RT-PCR

Genaxxon offers products for RNA PCR (RT-PCR = Reverse transcription polymerase chain reaction) which is still regarded as the most sensitive method for the detection and quantification of mRNA.

Compared to other conventional techniques for the quantification of mRNA - Northern Blot analysis and RNase protection assays - in RT-PCR much lower amounts of sample are needed.

The standard enyzme for the synthesis of cDNA is Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase >. M-MuLV Reverse Transcriptase shows no 3´ → 5´ exonuclease activity.
For the synthesis of cDNA Genaxxon offers also the cDNA One-Step RT-PCR Kit >.

Troubleshooting & complaints feeedback form >
Frequently asked questions (FAQs) >

Genaxxon offers products for RNA PCR (RT-PCR = Reverse transcription polymerase chain reaction) which is still regarded as the most sensitive method for the detection and quantification of mRNA.... read more »
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Products for RT-PCR

Genaxxon offers products for RNA PCR (RT-PCR = Reverse transcription polymerase chain reaction) which is still regarded as the most sensitive method for the detection and quantification of mRNA.

Compared to other conventional techniques for the quantification of mRNA - Northern Blot analysis and RNase protection assays - in RT-PCR much lower amounts of sample are needed.

The standard enyzme for the synthesis of cDNA is Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase >. M-MuLV Reverse Transcriptase shows no 3´ → 5´ exonuclease activity.
For the synthesis of cDNA Genaxxon offers also the cDNA One-Step RT-PCR Kit >.

Troubleshooting & complaints feeedback form >
Frequently asked questions (FAQs) >

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HotScriptase - heat stable reverse Transcriptase
HotScriptase RT-qPCR COVID-19 CDC Probe Assay Kit
The HotScriptase RT-qPCR SARS-CoV-2 CDC Probe Assay Kit is a real-time RT-PCR-based detection system for the targets N1 and N2 of the nucleocapsid from the SARS-CoV-2-Corona virus (2019-nCoV), as well as for the control target RNASeP....
From €205.00 *
HotScriptase - heat stable reverse Transcriptase
HotScriptase Probe RT-qPCR master mix without ROX
HotScriptase Probe master mix for RT-PCR without an isothermal intermediate step, thereby saving time and money: HotScriptase Probe master mix was successfully used by several customers for the detection of RNA viruses like Sika or...
From €20.00 *
OneStep RT-PCR Kit
cDNA One-Step RT-qPCR 2X GreenMastermix
The cDNA One-Step RT-qPCR 2X master mix was developed for quantitative real-time analyzes of RNA templates using EvaGreen®. The ready-to-use mixture is based on a genetically modified reverse transcriptase with improved thermal...
From €102.25 *
OneStep RT-PCR Kit
cDNA One-Step RT-qPCR 2X ProbeMastermix
The cDNA One-Step RT-qPCR 2X master mix was developed for quantitative real-time analyzes of RNA templates with double-labeled fluorescence probes. The ready-to-use mixture is based on a genetically modified reverse transcriptase with...
From €102.25 *
HotScriptase RT-PCR direct from cells
HotScriptase RT PCR from cell master mix
HotScriptase RT Cell, a 2-time master mix that has been specifically optimized for direct-from-cell applications. Skip the time-consuming and expensive RNA purification and cell lysis steps. With the new HotScriptase RT 2X Cell master...
From €35.00 *
HotScriptase RT-PCR without RNA purification
HotScriptase RT polymerase
"One-Step" RT-PCR without isothermal reverse transcription step. Using HotScriptase RT is very time and cost efficient as HotScriptase RT is a reverse transcriptase and a DNA polymerase combined in one highly thermostable enzyme. (find...
From €11.00 *
random Hexamer N6 Primer 5OD (160µg)
Random Hexamer Primer N6
Random Hexamers are short oligodeoxyribonucleotides of random sequence [d(N)6]. Primers are quality controlled (MS), purified (reversed phase HPLC), lyophilised and aliquoted and came with a guaranteed amount of at least 5OD (about...
From €21.66 * €28.88 *
Oligo dT20 Primer 5OD (130µg)
Oligo dT20 primer
Oligo (dT)20 are single stranded oligodeoxyribonucleotides containing only deoxythymine (dT) to be able to prime with the poly(A) tail of mRNA molecules. Primers are quality controlled (MS), purified (reversed phase HPLC), aliquoted and...
From €21.66 * €28.88 *
M-MuLV Reverse Transcriptase RNase H negative
M-MuLV Reverse Transcriptase RNase H negative
The Genaxxon M-MuLV Reverse Transcriptase, encoded by Moloney Murine Leukemia Virus (M-MuLV RT) and expressed in E.coli is an RNA-dependent DNA polymerase lacking the RNase H activity. The enzyme synthesizes the cDNA first strand from a...
From €110.14 *
OneStep RT-PCR Kit
cDNA One-Step RT-PCR Kit
The cDNA RT-PCR One-Step Kit is designed for performing highly sensitive and specific RT-PCR. The kit is based on a genetically engineered reverse transcriptase with enhanced thermal stability resulting in an increased specificity, high...
From €127.05 *
RNase Inhibitor - RNasin
RNase Inhibitor - RNasin
RNase inhibitor (ribonuclease inhibitor / RNasin) is a broad-spectrum inhibitor of RNases that is reversibly inactivated by binding to the inhibitor at a 1: 1 ratio with an association constant greater than 10E14. RNase A / B / C and...
From €76.18 *