Products for RT-PCR

Genaxxon offers a unique polymerase for RNA PCR (RT-PCR = Reverse transcription polymerase chain reaction) which is still regarded as the most sensitive method for the detection and quantification of mRNA.

Genaxxon´s HotScriptase RT Polymerase > has a combined RT-PCR and DNA polymerase function: For simultaneous reverse transcription and amplification during elongation, so that a time consuming and expensive isothermal intermediate RT-step is not needed anymore! HotScriptase RT polymerase is also characterized by extreme thermal stability.
Recommended amplicon size should be between 60-400 bp.

Over the past few years the development of novel chemicals and instruments for qPCR has meant that the real time RT-PCR has become established as the method of choice for quantifying changes in gene expression. Furthermore, the real time RT-PCR is the preferred method for validating the results from array analysis and other techniques to track changes in gene expression.

Compared to other conventional techniques for the quantification of mRNA - Northern Blot analysis and RNase protection assays - in RT-PCR much lower amounts of sample are needed. This method is even sensitive enough to allow the quantitation of RNA from a single cell: Use our HotScriptase RT Cell Master Mix >!

The standard enyzme for the synthesis of cDNA is Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase >. M-MuLV Reverse Transcriptase shows no 3´ → 5´ exonuclease activity.
For the synthesis of cDNA Genaxxon offers also the cDNA One-Step RT-PCR Kit >.

Troubleshooting & complaints feeedback form >
Frequently asked questions (FAQs) >

Genaxxon offers a unique polymerase for RNA PCR (RT-PCR = Reverse transcription polymerase chain reaction) which is still regarded as the most sensitive method for the detection and... read more »
Close window
Products for RT-PCR

Genaxxon offers a unique polymerase for RNA PCR (RT-PCR = Reverse transcription polymerase chain reaction) which is still regarded as the most sensitive method for the detection and quantification of mRNA.

Genaxxon´s HotScriptase RT Polymerase > has a combined RT-PCR and DNA polymerase function: For simultaneous reverse transcription and amplification during elongation, so that a time consuming and expensive isothermal intermediate RT-step is not needed anymore! HotScriptase RT polymerase is also characterized by extreme thermal stability.
Recommended amplicon size should be between 60-400 bp.

Over the past few years the development of novel chemicals and instruments for qPCR has meant that the real time RT-PCR has become established as the method of choice for quantifying changes in gene expression. Furthermore, the real time RT-PCR is the preferred method for validating the results from array analysis and other techniques to track changes in gene expression.

Compared to other conventional techniques for the quantification of mRNA - Northern Blot analysis and RNase protection assays - in RT-PCR much lower amounts of sample are needed. This method is even sensitive enough to allow the quantitation of RNA from a single cell: Use our HotScriptase RT Cell Master Mix >!

The standard enyzme for the synthesis of cDNA is Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase >. M-MuLV Reverse Transcriptase shows no 3´ → 5´ exonuclease activity.
For the synthesis of cDNA Genaxxon offers also the cDNA One-Step RT-PCR Kit >.

Troubleshooting & complaints feeedback form >
Frequently asked questions (FAQs) >

Close filters
 
  •  
  •  
from to
No results were found for the filter!
OneStep RT-PCR Kit
cDNA One-Step RT-PCR Kit
The cDNA RT-PCR One-Step Kit is designed for performing highly sensitive and specific RT-PCR. The kit is based on a genetically engineered reverse transcriptase with enhanced thermal stability resulting in an increased specificity, high...
From €115.50 *
HotScriptase - heat stable reverse Transcriptase
HotScriptase RT Cell Master Mix
HotScriptase RT Cell, a 2-time master mix that has been specifically optimized for direct-from-cell applications. Skip the time-consuming and expensive RNA purification and cell lysis steps. With the new HotScriptase RT 2X Cell master...
From €40.00 *
HotScriptase - heat stable reverse Transcriptase
HotScriptase RT Master Mix with GreenDye...
HotScriptase RT Master Mix for One-Step RT-PCR without isothermal reverse transcription step. Using HotScriptase RT is very time and cost efficienct as HotScriptase RT is a reverse transcriptase and a DNA Polymerase combined in one...
From €157.50 *
HotScriptase - heat stable reverse Transcriptase
HotScriptase RT Master Mix Probe without ROX
HotScriptase RT Master Mix for One-Step RT-PCR without isothermal reverse transcription step. Using HotScriptase RT is very time and cost efficienct as HotScriptase RT is a reverse transcriptase and a DNA Polymerase combined in one...
From €10.00 *
NEW
HotScriptase RT polymerase
HotScriptase RT polymerase
"One-Step" RT-PCR without isothermal reverse transcription step. Using HotScriptase RT is very time and cost efficient as HotScriptase RT is a reverse transcriptase and a DNA polymerase combined in one highly thermostable enzyme. (find...
From €10.00 *
M-MuLV Reverse Transcriptase RNase H negative
M-MuLV Reverse Transcriptase RNase H negative
The Genaxxon M-MuLV Reverse Transcriptase, encoded by Moloney Murine Leukemia Virus (M-MuLV RT) and expressed in E.coli is a RNA-dependent DNA polymerase laking the RNase H activity. The enzyme synthesizes the cDNA first strand from a...
From €103.82 *
Oligo dT20 Primer 5OD (130µg)
Oligo dT20 primer
Oligo (dT)20 are single stranded oligodeoxyribonucleotides containing only deoxythymine (dT) to be able to prime with the poly(A) tail of mRNA molecules. Primers are quality controlled (MS), purified (reversed phase HPLC), aliquoted and...
€28.88 *
random Hexamer N6 Primer 5OD (160µg)
Random Hexamer Primer N6
Random Hexamers are short oligodeoxyribonucleotides of random sequence [d(N)6]. Primers are quality controlled (MS), purified (reversed phase HPLC), lyophilised and aliquoted and came with a guaranteed amount of at least 5OD (about...
€28.88 *
RNase Inhibitor - RNasin
RNase Inhibitor - RNasin
RNase inhibitor (ribonuclease inhibitor / RNasin) is a broad-spectrum inhibitor of RNases that is reversibly inactivated by binding to the inhibitor at a 1: 1 ratio with an association constant greater than 10E14. RNase A / B / C and...
From €71.81 *