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Fast Blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye. Similarly, Fast Blue B is also used for α- and β-glucosidase activity.
Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens. It has been used as a spraying agent in thin layer chromatography for the identification of separated components or for the determination of lipase activity.
Synonyme: o-Dianisidine bis(diazotized) zinc double salt; Azoic Diazo No. 48; DBB; Diazo Blue B; Naphthanil Diazo Blue B.
Dye dontent: min. 90%
Appearance: Yellow, yellow-brown powder
Solubility: clear to slightly hazy, yellow to orange solution
Water: max. 10%
C14H12N4O2Cl2 x ZnCl2
MW = 475.47 g/mol
Sicherheits Hinweise / SafetyH-Sätze: H350
Klassifizierungen / ClassificationEC-Nr: 200-515-2
Dokumente - Protokolle - Downloads
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