FAST DNA polymerase (2X) MasterMix with dye
Order number: M3286.0001
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Genaxxon’s FAST DNA polymerase (2X) MasterMix with dye is the master mix of our FAST DNA polymerase (M3285) which is a robust enzyme, ideally suited for applications like genotyping and screening, amplifying with extreme speed of up to 2 seconds per 1kb, as well as high yield and consistency. FAST DNA polymerase has 5’-3’ exonuclease activity, but no 3’-5’ exonuclease (proofreading) activity. PCR products generated with this enzyme are A-tailed and can thus be cloned into TA cloning vectors. The extreme speed of Genaxxon’s FAST DNA polymerase allows the use of an extension rate of 4-8 kb/min. Genaxxon bioscience’s FAST DNA Polymerase (2X) MasterMix with dye includes all components needed for a fast PCR, except the primers. Besides this, it includes an inert red tracking dye for gel electrophoresis. Therefore, upon completion of the PCR, the reaction is ready for direct loading onto an agarose gel without the need of adding an additional loading buffer.
Mastermix of our high speed DNA polymerase for FAST PCR
Technical Data:
Specifications:
Genaxxon’s FAST DNA polymerase (2X) MasterMix with dye is supplied as a convenient 2x master mix, including an inert red tracking dye for electrophoresis, containing all required components for fast PCR, except specific primers. Final concentration of MgCl2 will be 3mM. One mL is suitable for 80 reactions of 25µL (or 100 reactions of 20µL).
Extension rate: up to 30 kb/min. at 72°C
5’-3’ exonuclease activity: Yes
Extra addition of A: Yes
3’-5’ exonuclease activity: No
Nuclease contamination: No
Protease contamination: No
RNase contamination: No
application:
colony screens, genotyping, fast routine PCR, TA cloningSource
recombinant, purified from E.coliSicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02Dokumente - Protokolle - Downloads
Here you will find information and further literature on FAST DNA polymerase (2X) MasterMix with dye. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
Dokumente - Protokolle - Downloads
2 seconds extention time for targets below 1 kb and 15 seconds per kb for target DNA up to 5kb at 72ºC for extension.
Corona PCR gargling test Product only available on request 