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This tablets are designed for the purification of histidine tagged and non-histidine tagged recombinant and native proteins using IMAC chromatography. The phosphate buffer may be used for other types of affinity chromatography methods. Immobilized metal ion affinity chromatography (IMAC) is a widely used separation method for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also some untagged recombinant or native proteins. Genaxxon's IMAC phosphate and elution buffer tablets have been developed exclusively for use in this method. IMAC buffer tablets are supplied in bottles and in blister packs. One tablet of phosphate buffer dissolved in deionized water yields a 300mM sodium chloride, 50mM sodium phosphate buffer with pH8.0 at 22°C. One tablet of elution buffer yields a 300mM sodium chloride, 300mM imidazole, 50mM sodium phosphate buffer with pH8.0 at 22°C. Features: Dissolve and go, Exactly pre-weight tablets, just mix elution buffer with phosphate buffer to achieve desired imidazole concentration, ultrapure, low UV280nm absorbance imidazole.
pre-weight ready-to-use certified
Composition: Tris: 300mM Sodium chloride; 50mM Sodium phosphate, pH8.0 +/- 0.05 (22°C). One tablet is enough for 400mL buffer. Tablets in blister pack.
application:for purification of his-tagged proteins
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-04-05
Dokumente - Protokolle - Downloads
Here you will find information and further literature on IMAC phosphate buffer. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.