Ready-to-Use Buffers: Tablets and Powders

TAE buffer is used for nucleic acid electrophoresis on agarose gels under low voltage conditions. Ref.: Loening U.E. (1967) Biochem. J., 102, 251, Ogden R.C. and Adams D.A. (1987) Methods Enzymol., 152, 61. TAE buffer is the most commonly used running buffer for agarose gels. Originally, this buffer system was developed for polyacrylamide gel electrophoresis with a slightly different composition (40mM Tris; 20mM; NaOAc; 2mM EDTA-Na2; pH7.8). For stabilizing the secondary structure of RNA, sodium acetate was included. Today, TAE is used in a modified composition (40mM Tris-acetate; 1mM EDTA-Na2; ~pH8.5). TAE has a lower buffering capacity than TBE, but double-stranded, linear DNA migratesapproximately 10% faster through TAE than TBE with the same resolution. The resolution of supercoiled DNA is better in TAE than TBE. Because of its low buffering capacitiy, it may become exhausted during long periods of time at high current. Therefore TAE should be replaced during extended electrophoresis or should be recirculated. An advantage of TAE over TBE is the absence of interactions with agarose, resulting in a higher yield of nucleic acids in preparative agarose gel electrophoresis. Usually TAE is made up as a 50X concentrated stock solution and employed in an 1X or 0,5X working concentration.

Precisely weighed ready-to-use PBS buffer tablets offered in plastic bottles or in practical blister packs. - no awkward weighing anymore- no more time-consuming pH adjustment- simply dissolve the tablet completely in demineralized water and use the buffer 1 tablet dissolved in 500mL of deionized water yields: 0.01M Phosphate buffer, 0.0027M KCl, 0.14M NaCl, pH7.4 at 25°C. Amoung biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns. Our PBS is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches. There are different standard-sized packages and final volumes range from 100mL to 100 litres. Choose from different pH (7.2 or 7.4), from PBS with our without potassium, or with or without Tween. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >).PBS with Tween™20, pH7.4 (D2003 (100mL and 500mL) > and (D2004 (1L) >).PBS, pH7.2 (D2034 >) PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

Precisely weighed ready-to-use PBS tablets with Tween®20 offered in plastic bottles or in practical blister packs. - no awkward weighing anymore- no toilsome pipetting/weighing of Tween®20 anymore- no more time-consuming pH adjustment- simply dissolve the tablet completely in demineralized water and use the buffer Ready-to-use PBS buffer tablets with Tween®20 containing the non-ionic detergent Tween®20 which acts through blocking. It has the ability to reduce non-specific binding and protein-protein interaction during the wash step in protein and immunoassay procedures such as ELISA and Western blotting. By decreasing the non-specific binding and staining makes interpretation of ELISA results and Western blots easier. Each tablet dissolved in deionized water yields a 0.14M sodium chloride 0.0027M potassium chloride, 0.05% Tween®20, 0.01M phosphate buffer with pH7.4 at 25°C. Final buffer volumes are: 100mL and 500mL (D2003 >) or 1L (D2004 >). Our ready-to-use tablets are also available as PBS tablets without Tween®20, as TBS or as IMAC tablets for affinity chromatography. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >).PBS with Tween™20, pH7.4 (D2003 (100mL and 500mL) > and (D2004 (1L) >).PBS, pH7.2 (D2034 >) PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

ready-to-use PBS buffer tablets. Each tablet dissolved in 1000mL of deionized water yields: 0.01M Phosphate buffer, 0.0027M KCl, 0.14M NaCl, pH7.2 at 25°C. No time consuming weighing or measuring of pH necessary anymore. You can get rid of the whole validation process and you will have no need for scales or pH-meters anymore. Amoung biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns. Our PBS is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches. There are different standard-sized packages and final volumes range from 100mL to 100 litres. Choose from different pH (7.2 or 7.4), from PBS with our without potassium, or with and without Tween®. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >).PBS with Tween™20, pH7.4 (D2003 (100mL and 500mL) > and (D2004 (1L) >).PBS, pH7.2 (D2034 >) PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

TAE buffer is used for nucleic acid electrophoresis on agarose gels under low voltage conditions. Ref.: Loening U.E. (1967) Biochem. J., 102, 251, Ogden R.C. and Adams D.A. (1987) Methods Enzymol., 152, 61. TAE buffer is the most commonly used running buffer for agarose gels. Originally, this buffer system was developed for polyacrylamide gel electrophoresis with a slightly different composition (40mM Tris; 20mM; NaOAc; 2mM EDTA-Na2; pH7.8). For stabilizing the secondary structure of RNA, sodium acetate was included. Today, TAE is used in a modified composition (40mM Tris-acetate; 1mM EDTA-Na2; ~pH8.5). TAE has a lower buffering capacity than TBE, but double-stranded, linear DNA migratesapproximately 10% faster through TAE than TBE with the same resolution. The resolution of supercoiled DNA is better in TAE than TBE. Because of its low buffering capacitiy, it may become exhausted during long periods of time at high current. Therefore TAE should be replaced during extended electrophoresis or should be recirculated. An advantage of TAE over TBE is the absence of interactions with agarose, resulting in a higher yield of nucleic acids in preparative agarose gel electrophoresis. Usually TAE is made up as a 50X concentrated stock solution and employed in an 1X or 0,5X working concentration.

Contents of 1 pouch dissolved in deionized water and made up to 500mL/1000mL yields: 2.0M Tris acetate buffer, 0.05M EDTA, pH8.3 at 25°C. In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis.TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels. TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide. Medicago’s TBE and TAE buffers are supplied as a pre-weighed powder mix in sealed pouches giving 1000mL of 1X, 5X or 10X Tris-borate-EDTA buffer or 50X Tris-acetate-EDTA buffer with pH 8.3 at 25°C.

This buffer is the most widely used buffer for electrophoresis on agarose or acrylamide gels, it is particularly well suited for high-voltage long migration conditions. pH8.3 +/- 0.2.

This buffer is the most widely used buffer for electrophoresis on agarose or acrylamide gels, it is particularly well suited for high-voltage long migration conditions. pH8.3 +/- 0.2. Delivery in PE bottles / canister.

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.89M Tris-borate, 0.02M EDTA, pH8.3 at 25°C. In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis.TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels. TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide. Medicago’s TBE and TAE buffers are supplied as a pre-weighed powder mix in sealed pouches giving 1000mL of 1X, 5X or 10X Tris-borate-EDTA buffer or 50X Tris-acetate-EDTA buffer with pH 8.3 at 25°C. You will find more ready-to-use buffers here: ready-to-use buffers >

10 bags of a 10-fold Tris-EDTA buffer (pH7.4) - ready-to-use Tris/HCl-EDTA powder mixture for 1L ready-to-use buffer solution of pH7.4. This TE buffer is composed by Tris, a buffering agent and EDTA. EDTA has the ability to prevent degradation of DNA and RNA by chelating magnesium- or other divalent metal ions.Tris-EDTA-based solutions break protein cross-links and can therefore unmask antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections. Treatment with TE buffer enhances the staining intensity of antibodies in the immuno-histochemical detection of certain proteins. TE buffer powder is supplied as exactly pre-weighed powder in pouches. To prepare the buffer, the content of a bag is simply dissolved in 1L of deionized water. This results in a 0.1M Tris/HCl, 0.01M EDTA buffer of pH7.4 at 25°C.       T

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.5M Tris buffered saline, 1.38M NaCl, 0.027M KCl, pH8.0 at 25°C.

Tris-EDTA buffer 1X concentrated, Buffer Grade. pH7.5 +/- 0.2. TE buffer is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. They are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA buffer solution, pH 8.0 may also be used as a washing buffer. Reference: Sambrock J., Fritsch E.F. and Maniatis T (1989) Molecular Cloning, A Laboratory Manual, 2nd Edition, Cold Spring Harbor, New York.

Tris-EDTA buffer 1X concentrated, molecular biology grade. pH8.0 +/- 0.2. TE buffer is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. They are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA buffer solution, pH 8.0 may also be used as a washing buffer. Reference: Sambrock J., Fritsch E.F. and Maniatis T (1989) Molecular Cloning, A Laboratory Manual, 2nd Edition, Cold Spring Harbor, New York.

1 tablet dissolved in 100mL of deionized water yields: 0.05M Sodium Carbonate-bicarbonate buffer, pH9.6 at 25°C. No time consuming and expenisve calibration / pH-measuring necessary. Just dissolve buffer talets in water and use buffer solution. You will find more ready-to-use buffers here: ready-to-use buffers >

1 tablet dissolved in 100mL of deionized water yields: 0.05M Sodium Carbonate-bicarbonate buffer, 0.05% Sodium Azide, pH9.6 at 25°C.

Content of 1 pouch dissolved in deionized water and made up to 500mL/1000mL yields: 0.5M EDTA, pH8.0 at 25°C. EDTA (ethylene-diamine-tetraacetic acid) is a chelating agent widely used in molecular biology to sequester divalent and trivalent metal ions such as calcium and magnesium. This ability prevents DNA and RNA degradation as metal-dependent enzymes acting as nucleases become deactivated. A fully deprotonated EDTA molecule will bind directly to the metal ion making the buffer suitable for adding to stored blood as an anti-coagulant to bind Ca2+ ions. Furthermore, EDTA is useful for cell culture procedures as it prevents clumping of cells in liquid suspension and detaches adherent cells when passaging. Genaxxon’s EDTA buffer is supplied in two sizes with pouches giving 500mL or 1000mL of 0.50M EDTA buffer with pH8.0 at 25°C when the contents of one pouch is dissolved in deionized water.

This tablets are designed for the purification of histidine tagged and non-histidine tagged recombinant and native proteins using IMAC chromatography. The phosphate buffer may be used for other types of affinity chromatography methods. Immobilized metal ion affinity chromatography (IMAC) is a widely used separation method for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also some untagged recombinant or native proteins. Genaxxon's IMAC phosphate and elution buffer tablets have been developed exclusively for use in this method. IMAC buffer tablets are supplied in bottles and in blister packs. One tablet of phosphate buffer dissolved in deionized water yields a 300mM sodium chloride, 50mM sodium phosphate buffer with pH8.0 at 22°C. One tablet of elution buffer yields a 300mM sodium chloride, 300mM imidazole, 50mM sodium phosphate buffer with pH8.0 at 22°C. Features: Dissolve and go, Exactly pre-weight tablets, just mix elution buffer with phosphate buffer to achieve desired imidazole concentration, ultrapure, low UV280nm absorbance imidazole.

This tablets are designed for the purification of histidine tagged and non-histidine tagged recombinant and native proteins using IMAC chromatography. The phosphate buffer may be used for other types of affinity chromatography methods. Immobilized metal ion affinity chromatography (IMAC) is a widely used separation method for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also some untagged recombinant or native proteins. Genaxxon's IMAC phosphate and elution buffer tablets have been developed exclusively for use in this method. IMAC buffer tablets are supplied in bottles and in blister packs. One tablet of phosphate buffer dissolved in deionized water yields a 300mM sodium chloride, 50mM sodium phosphate buffer with pH8.0 at 22°C. One tablet of elution buffer yields a 300mM sodium chloride, 300mM imidazole, 50mM sodium phosphate buffer with pH8.0 at 22°C. Features: Dissolve and go, Exactly pre-weight tablets, just mix elution buffer with phosphate buffer to achieve desired imidazole concentration, ultrapure, low UV280nm absorbance imidazole.

Ready-to-use Sodium phosphate buffer in a sealed bag. Content of 1 bag dissolved in deionized water and made up to 1000mL yields a 100mM sodium phosphate buffer from pH6.0 to pH8.0 at 25°C. This Sodium Phosphate buffer is provided in different formulations in order to suit most biochemical and molecular biology applications. Choose a buffer with a pH between 6.0 and 8.0 and a molarity that fits your laboratory procedures. All our Sodium phosphate buffers are supplied as exactly pre-weighed powder in sealed pouches. How to prepare buffer solution: Empty one pouch of NaPi buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 200mL to 300mL of deionized water for a 1000mL pouch and stir the solution for a few minutes. Adjust the volume up to 1000mL, stir and the buffer solution is ready to use. Other phosphate-based ready-to-use buffers: PBS without Potassium (0.01M) (D2033 >).PBS with Tween™20, pH7.4 (D2003 (100mL and 500mL) > and (D2004 (1L) >).PBS, pH7.2 (D2034 >) PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

Contents of 1 pouch dissolved in deionized water and made up to 1000mL yields: 1M Sodium phosphate buffer, pH7.2 at 25°C. Other phosphate-based ready-to-use buffers:: PBS without Potassium (0.01M) (D2033 >).PBS with Tween™20, pH7.4 (D2003 (100mL and 500mL) > and (D2004 (1L) >).PBS, pH7.2 (D2034 >) PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >). Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >