Non-Ionic Detergents
n-Decyl-b-maltoside (DM) >99.0% highly purified
n-Decyl-b-maltoside (DM) >99.5% for crystallography
Highly pure Dodecyl-ß-maltopyranoside (DDM). Detergent to solubilize membrane assoziated proteins. n-Dodecyl-β-D-maltoside (DDM), a water-soluble nonionic detergent, for isolation and stabilization of hydrophobic membrane proteins. n-Dodecyl-β-D-maltoside is a gentle detergent that preserves protein activity better than many other detergents, such as CHAPS or octyl-β-glucoside.
n-Dodecyl-ß-maltoside (DDM) >99.5% especially suitable for use in crystallography. n-Dodecyl-β-D-maltoside (DDM), is a water-soluble non-ionic detergent, for isolation and stabilization of hydrophobic membrane proteins. n-Dodecyl-β-D-maltoside is a gentle detergent that preserves protein activity better than many other detergents, such as CHAPS or octyl-β-glucoside. The protein-sparing properties of this high-purity DDM allow it to be used, for example, in nasal sprays, as the DDM leads to improved mucus clearance.
MEGA-9 is a nonionic detergent which is often used to solubilize membrane proteins. It has a critical micelle concentration (CMC) of 20 mM under no-salt conditions and CMCs ranging from 2 to 17. mM under high and low salt conditions for a variety of salts.
n-Nonyl b-maltoside (NM); Assay: >99.5% for crystallography.
n-Nonyl b-maltoside (NM); Assay: >99% highly purified.
Octyl glucoside (n-octyl-β-D-glucoside) is a detergent frequently used to solubilise integral membrane proteins for studies in biochemistry. It has become one of the most important detergents for purification of membrane proteins because it generally does not denature the protein and can readily be removed from final protein extracts. Above its critical micelle concentration of 0.7%, it was noted as the best detergent for improving selectivity of immunoprecipitation of phosphotyrosine modified proteins. This detergent has also been shown to rapidly inactivate infective HIV at concentrations above its CMC (about 0.7% (w/v)).
Octyl glucoside (n-octyl-β-D-glucoside) is a detergent frequently used to solubilise integral membrane proteins for studies in biochemistry. It has become one of the most important detergents for purification of membrane proteins because it generally does not denature the protein and can readily be removed from final protein extracts. Above its critical micelle concentration of 0.7%, it was noted as the best detergent for improving selectivity of immunoprecipitation of phosphotyrosine modified proteins. This detergent has also been shown to rapidly inactivate infective HIV at concentrations above its CMC (about 0.7% (w/v)).
Technical data can be found under Detail view.
Polyethylene glycol 400, PEG, Polyoxyethylen, Polyglycol.
PEG 1000, Polyglycol 1000, HO(C2H4N)nH
Polyethylene glycol 4000 (PEG 4000) is for example used for the fusion of bacterial spheroplasts or the fractionated precipitation of proteins.
Polyethylene glycols (PEGs) can be readily synthesised through the anionic ring-opening polymerisation of ethylene oxide to produce substances with a wide range of molecular weights and a variety of end groups. Cross-linked PEG can have a high water content and form ‘hydrogels’. As PEGs do not trigger an immune response, they are suitable for a wide range of biological applications.Polyethylene glycol (PEG) is used for purposes as diverse as virus/phage enrichment, the fusion of plant protoplasts or the fractionated precipitation of proteins. Furthermore, ligation efficiency can be increased through ‘macromolecular crowding’, i.e. the concentration of molecules in solution. A 40% PEG 6000 solution was used as the stock solution.
PEG 8000 also known as Polyethylene glycol 8000 has a pronounced hydration capacity and is structurally flexible. Due to its biocompatibility and the absence of steric hindrances in its structure, PEG is often added to protein containing solution to stabilize the protein or to increase the solubility of the protein.In molecular biology applications Polyethylene glycol 8000 is used as buffer component for transformation of yeast, or for buffers to isolate or to concentrate viruses.Cloning:Especially for the cloning with 'blunt-ended' DNA, the concentration of 'blunt'-DNA-ends plays a crucial role for the successful cloning. Substances, that increase the so-called 'macromolecular crowding' concentrate the DNA locally and increase the possibility, that DNA ends will meet and thereby improve the cloning result. A final concentration of 5-15% PEG 8000 is often recommended for ligation reactions, but the optimal concentration can vary depending on the specific reaction and enzyme used. E.g. the kinetic of the ligation in the cloning of DNA-fragments into bacteriophage M13-vectors can be improved by the inclusion of 5% polyethylene glycol 8000 (PEG 8000).Prevents premature circularization: While a 5% concentration is often optimal, exceeding 10% can inhibit transformation efficiency. Stock solutions of PEG 8000 (40%) are prepared with deionized nuclease-free water and stored at -20°C in small aliquots.
n-Tridecyl-ß-maltoside (TDM) >99.5% for crystallography.
Technical data can be found under Detail view.