p-Nitrophenylphosphate (20mg) tablets

p-Nitrophenylphosphate (20mg) tablets


Order number: D2011.0024

Shipping: shipped on wet ice, store at -20°C in the dark!
CAS-Nr: 4264-83-9

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24 respective 100 ready-to-use p-Nitrophenylphosphate (pNPP) tablets with 20mg... more
Product information "p-Nitrophenylphosphate (20mg) tablets"

24 respective 100 ready-to-use p-Nitrophenylphosphate (pNPP) tablets with 20mg p-Nitrophenylphosphate. Each tablet dissolved in 20mL deionized water yields a solution containing 1.0mg/mL pNPP, 0.2M Tris buffer.

Genaxxon's pNPP substrate is specifically developed for immunoassay procedures and it is ideal for phosphate-based ELISA methods. The substrate is also used as a chromogenic non-specific substrate in alkaline and acid phosphatase assays. Its soluble end-product is yellow and can be spectrophotometrically read at 405 to 410 nm. The reaction may be stopped with 3M NaOH.

The pNPP substrate is supplied as pre-weighed tablets in bottles or in convenient blister packs, each tablet containing 5mg or 20mg of substrate.

How to use:
Deposit one tablet of the pNPP substrate in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized water in the appropriate amount to reach the required concentration for the assay. Stir until full dissolution and the substrate solution is ready to use.

One tablet dissolved in 20mL of substrate buffer results in a 1mg/mL solution.

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Our comment on "p-Nitrophenylphosphate (20mg) tablets"
Certified Exactly pre-weighed tablets - ready-to-use Formulated from analytical grade reagents Specifically developed for immunoassays Chromogenic substrate Reproducibility from lot to lot
Specifications: tablets with 20mg p-Nitrophenylphosphate Weight of tablet: 200mg +/-1mg... more
 

Technical Data:

Specifications:
tablets with 20mg p-Nitrophenylphosphate

Weight of tablet: 200mg +/-1mg
Content: 20mg pNPP +/-5mg
Appearance: white to sligthly yellow tablets

application:

Immunoassays Chromogenic substrate for phosphatase assays Histochemistry procedures Recommended for ELISA procedures

Source

synthetic

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

CAS-Nr: 4264-83-9
eclass-Nr: 32-16-04-05
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

Here you will find information and further literature on p-Nitrophenylphosphate (20mg) tablets. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

 
 
 
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

Common buffers used in ELISA tests:
a. 0.1M glycine, pH10.4, 1mM MgCl2, 1mM ZnCl2.
b. 1M diethanolamine, pH9.8, 0.5mM MgCl2.
c. 0.2M Tris, pH8.8, 5mM MgCl2.

Preparation of a sterile solution:
- Prepared solution may be sterilized by filtration (0,2µm). The solution has to be stored at -20°C in aliquots!

 

 
 
 
Literatur .... more

Referenzen..

Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.

Quelle/Source: NCBI PubMed >

Pten controls B‐cell responsiveness and germinal center reaction by regulating the expression of IgD BCR
Corinna S Setz, Ahmad Khadour, Valerio Renna, Joseena Iype, Eva Gentner, Xiaocui He, Moumita Datta, Marc Young, Lars Nitschke, Jürgen Wienands, Palash C Maity, Michael Reth, Hassan Jumaa
EMBO J. 2019 Jun 3; 38(11): e100249. Published online 2019 Apr 23. doi: 10.15252/embj.2018100249
PMCID: PMC6545559

PI3K-Mediated Blimp-1 Activation Controls B Cell Selection and Homeostasis

Corinna S. Setz, Eva Hug, Ahmad Khadour, Hend Abdelrasoul, Mayas Bilal, Elias Hobeika, Hassan Jumaa

Cell Rep. 2018 Jul 10; 24(2): 391–405. Published online 2018 Jul 11. doi: 10.1016/j.celrep.2018.06.035
PMCID: PMC6057491