G418 Powder (min. 98% DC)
Order number: M3117.0001
Shipping: shipped at RT, store at +2°C to +8°C; store tightly sealed and protect from light.CAS-Nr: 108321-42-2
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
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G418 also known Geneticin(TM) is an aminoglycoside antibiotic that inhibits protein synthesis in mammalian cells by interfering with ribosomal function. Geneticin shows a structure similar to gentamycin > and kanamycin >, and finds application in the selection of stably transformed cells expressing the neomycin resistance gene (aminoglycoside) of transposons Tn 5 or Tn 601.
Because G418 is toxic for many cells, the optimum concentration for each cell type has to be determined. It is usually between 50ug/mL and 1000μg/mL. At a concentration of 500μg/mL, for example, 70% of HepG2 cells are killed after incubation for one week and 100% will stop their growth.Since potency of the antibiotic can vary from batch to batch, it is recommended to create large stocks of a batch tested to standardize the system.
Cells divide still once or twice in the presence of the lethal dose of G418, so that a selection effect will be visible only after several days. Geneticin is a registered trademarks of Gibco a Division of Life Technologies.
Genaxxon's G418 is an alternative to ThermoFisher / Invitrogen product no. 10131035 and 10131027, or a replacement for BioChrom product no.: A 2812.
G418 inhibits protein synthesis in mammalian cells by interfering with ribosomal function.
Technical Data:
Specifications:
Assay: min. 98% (TLC)
Potency (calc. on tried substance): min. 670µg/mg
Appearance: white solid substance; soluble in water and aqueous buffers
Water: max. 12.5%
C20H40N4O10x2H2SO4
MW = 692.7 g/mol
G418 (as powder) is stable at +2°C to +8°C and as solution at -20°C for at least 2 years.
application:
selection of stably transformed cells expressing the neomycin resistance gene (aminoglycoside) of transposons Tn 5 or Tn 601.Sicherheits Hinweise / Safety
H-Sätze: H301R-Sätze: R61
Gefahrstoffkürzel: T
Klassifizierungen / Classification
CAS-Nr: 108321-42-2eclass-Nr: 32-17-01-90
Dokumente - Protokolle - Downloads
Here you will find information and further literature on G418 Powder (min. 98% DC). For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
Referenzen..
Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
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