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Cell Separation reagents - Cell Separation reagents
Cell separation media are used for the separation of target cells from a complex mixture, such as whole blood or tissue samples, based on density gradients.
The method of density gradient centrifugation separates the specific cell type of interest from other cells or biological particles based on the density range of the gradient media and of the sample particles.
Caesium chloride (min. 99.9 %), Molecular biology grade
€854.44*
From
€126.79*
Caesium chloride for density centrifµgation. Ideal for the isolation of highly pure RNA without contamination with RNase, or other proteins and DNA. Ref.: Miller H. (1987) Methods Enzymol., 152, 145, Dorin M. and Bornecque C.A. (1995) BioTechniques, 18,90.
Variants from €126.79*
Caesiumchlorid ultrapure (min. 99.999%)
€1,149.75*
Caesium chloride for density centrifugation. Ideal for the isolation of highly pure RNA without contamination with RNase, or other proteins and DNA. Ref.: Miller H. (1987) Methods Enzymol., 152, 145, Dorin M. and Bornecque C.A. (1995) BioTechniques, 18,90.
Ficoll(R) 400 - Polysucrose 400
From
€57.16*
Suited for preparation of continuous and discontinuous density gradients. For stabilisation of membrane-bound particles, isolation of lymphocytes. Better osmotic properties than sucrose. Ficoll is also part of Denhardt's solution. As a supplement in gel loading buffers it increases density of the buffer for faster settlement of the solution in the gel caves while loading the gel.
LEUCO-Human - Cell separation medium
From
€17.79*
The Genaxxon LEUCO-Human centrifugation reagent is specially designed to separate human leucocytes from other blood cells. The separation medium it optimized to follow the selection criteria for density gradient centrifugation agents used in cell sorting. Density: 1.077 g/mL: Forming stable gradients over desired range. pH and osmolality are easily adjustable. Solutions are not too viscous in case of high density. This medium does not cause functional or morphological changes in the applied biological material and it does not penetrade biological membranes.
LEUCO-Mouse - Cell separation medium
From
€32.22*
The Genaxxon centrifugation reagents are optimised to follow the selection criteria for density gradient centrifugation agents used in cell sorting. Density: 1.086 g/mL: Forming stable gradients over desired range. pH and osmolality are easily adjustable. Solutions are not too viscous in case of high density. They do not cause functional or morphological changes in the applied biological material and they do not penetrade biological membranes.
Lympho-Paque - Cell separation medium
From
€16.21*
Genaxxon's Lympho-Paque Cell Separation Medium (C4754) is a sterile, ready-to-use medium for the simple and rapid isolation of lymphocytes with high yield from peripheral blood by ultracentrifugation. Lympho-Paque has a density of 1.077g/mL and maintains viability and representative distribution of B and T lymphocytes.
Isolation of cells or subcellular particles is often the first step in gene expression studies or diagnostic studies. Besides bio-specific separation methods, the most commonly used methods are physical separation methods based on physical differences such as size and charge of the particles to be separated. Special density gradient or separation media are used for this purpose.
Genaxxon's Ficoll-based Lympho-Paque cell separation medium is an excellent separation medium that meets all criteria for optimal laboratory results. It guarantees a high yield in the isolation of lymphocytes and other mononuclear cells from anticoagulated blood by an ultracentrifugation step.