Electrophoresis - DNA electrophoresis

SafeGel red stain is an ultra sensitive, extremely stable fluorescent dye designed to replace the toxic and possibly mutagenic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. SafeGel red stain is far more sensitive than EtBr without requiring a destaining step. SafeGel red stain is far less toxic and mutagenic compared to EtBr while both show virtually the same UV-spectra, so you can directly replace EtBr with SafeGel red stain without changing your existing imaging system. SafeGel can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. SafeGel can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. SafeGel is also compatible with downstream DNA manipulations such as digestion with a restriction enzyme, Southern blotting techniques and clonings. A series of safety tests has confirmed that SafeGel is noncytotoxic, nonmutagenic and nonhazardous even at concentrations above the working concentrations used in gel staining. As a result, SafeGel can be safely disposed in regular trash, providing convenience and reducing cost in waste disposal. This fluorescent dye is supplied as a 10,000X solution in water. For customers who look for large pack size, we offer a cost-saving bulk pack size of 2mL, 5mL or 10mL (M3193.1010). Read in our blog why you should use Genaxxon's SafeGel now. For high-class agarose gels we offer the Genaxxon standard agarose LE > or our speciality agaroses for high resolution Tiny (M3046) > and Tiny HT (M3047) >.

SafeGel green stain is an ultra sensitive, extremely stable fluorescent dye designed to replace the toxic and possibly mutagenic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. SafeGel green stain is far more sensitive than EtBr without requiring a destaining step. SafeGel green stain is far less toxic and mutagenic compared to EtBr while both show virtually the same UV-spectra, so you can directly replace EtBr with SafeGel green stain without changing your existing imaging system. SafeGel can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. SafeGel can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. SafeGel is also compatible with downstream DNA manipulations such as digestion with a restriction enzyme, Southern blotting techniques and clonings. A series of safety tests has confirmed that SafeGel is noncytotoxic, nonmutagenic and nonhazardous even at concentrations above the working concentrations used in gel staining. As a result, SafeGel can be safely disposed in regular trash, providing convenience and reducing cost in waste disposal. This fluorescent dye is supplied as a 10,000X solution in water. For customers who look for large pack size, we offer a cost-saving bulk pack size of 2mL, 5mL or 10mL (M3193.1010). For high-class agarose gels we offer the Genaxxon standard agarose LE > or our speciality agaroses for high resolution Tiny (M3046) > and Tiny HT (M3047) >. * * *Discover our NEW Product: More Info PDF >

GelRed(TM) is an ultra sensitive, extremely stable fluorescent dye designed to replace the toxic and possibly mutagenic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed(TM) is far more sensitive than EtBr without requiring a destaining step. GelRed(TM) is far less toxic and mutagenic compared to EtBr while both show virtually the same UV-spectra, so you can directly replace EtBr with GelRed(TM) without changing your existing imaging system. GelRed(TM) can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. GelRed(TM) can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. GelRed(TM) is also compatible with downstream DNA manipulations such as digestion with a restriction enzyme, Southern blotting techniques and clonings. A series of safety tests has confirmed that GelRed(TM) is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed(TM) can be safely disposed in regular trash, providing convenience and reducing cost in waste disposal. This fluorescent dye is supplied as a 10,000X solution in water. For customers who look for large pack size, we offer a cost-saving bulk pack size of 2mL, 5mL or 10mL (M3199.1010). For high-class agarose gels we offer the Genaxxon standard agarose LE > or our speciality agaroses for high resolution Tiny (M3046) > and Tiny HT (M3047) >.

(2,7-Diamino-10-ethyl-9-phenylphenanthridium bromide) Ethidium bromide is an intercalating agent for nucleic acids. It is widely used for staining of nucleic acids after electrophoresis on agarose or acrylamide gels and for fluorescent labeling on a cesium chloride gradient. As Ethidium bromide is a powerful mutagen and moderately toxic it is highly recommended to use gloves and the GENAXXON bioscience ready-to-use solutions. Reference: Lunn G. and Sansone E.B. (1987) Anal. Biochem., 162, 453 - Sambrock J., Fritsch & E.F. and Maniatis T (1989) Molecular Cloning, A Laboratory Manual, 2nd Edition, Cold Spring Harbor, New York.

TAE buffer is used for nucleic acid electrophoresis on agarose gels under low voltage conditions. Ref.: Loening U.E. (1967) Biochem. J., 102, 251, Ogden R.C. and Adams D.A. (1987) Methods Enzymol., 152, 61. TAE buffer is the most commonly used running buffer for agarose gels. Originally, this buffer system was developed for polyacrylamide gel electrophoresis with a slightly different composition (40mM Tris; 20mM; NaOAc; 2mM EDTA-Na2; pH7.8). For stabilizing the secondary structure of RNA, sodium acetate was included. Today, TAE is used in a modified composition (40mM Tris-acetate; 1mM EDTA-Na2; ~pH8.5). TAE has a lower buffering capacity than TBE, but double-stranded, linear DNA migratesapproximately 10% faster through TAE than TBE with the same resolution. The resolution of supercoiled DNA is better in TAE than TBE. Because of its low buffering capacitiy, it may become exhausted during long periods of time at high current. Therefore TAE should be replaced during extended electrophoresis or should be recirculated. An advantage of TAE over TBE is the absence of interactions with agarose, resulting in a higher yield of nucleic acids in preparative agarose gel electrophoresis. Usually TAE is made up as a 50X concentrated stock solution and employed in an 1X or 0,5X working concentration.

TAE buffer is used for nucleic acid electrophoresis on agarose gels under low voltage conditions. Ref.: Loening U.E. (1967) Biochem. J., 102, 251, Ogden R.C. and Adams D.A. (1987) Methods Enzymol., 152, 61. TAE buffer is the most commonly used running buffer for agarose gels. Originally, this buffer system was developed for polyacrylamide gel electrophoresis with a slightly different composition (40mM Tris; 20mM; NaOAc; 2mM EDTA-Na2; pH7.8). For stabilizing the secondary structure of RNA, sodium acetate was included. Today, TAE is used in a modified composition (40mM Tris-acetate; 1mM EDTA-Na2; ~pH8.5). TAE has a lower buffering capacity than TBE, but double-stranded, linear DNA migratesapproximately 10% faster through TAE than TBE with the same resolution. The resolution of supercoiled DNA is better in TAE than TBE. Because of its low buffering capacitiy, it may become exhausted during long periods of time at high current. Therefore TAE should be replaced during extended electrophoresis or should be recirculated. An advantage of TAE over TBE is the absence of interactions with agarose, resulting in a higher yield of nucleic acids in preparative agarose gel electrophoresis. Usually TAE is made up as a 50X concentrated stock solution and employed in an 1X or 0,5X working concentration.

Contents of 1 pouch dissolved in deionized water and made up to 500mL/1000mL yields: 2.0M Tris acetate buffer, 0.05M EDTA, pH8.3 at 25°C. In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis.TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels. TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide. Medicago’s TBE and TAE buffers are supplied as a pre-weighed powder mix in sealed pouches giving 1000mL of 1X, 5X or 10X Tris-borate-EDTA buffer or 50X Tris-acetate-EDTA buffer with pH 8.3 at 25°C.