BCIP, molecular biology grade



Order number: M3196.0500

Shipping: shipped at RT, stored at -20°C
CAS-Nr: 6578-06-9

Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

€61.43 *

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BCIP (5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt) is a chromogenic substrate for... more
Product information "BCIP, molecular biology grade"

BCIP (5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt) is a chromogenic substrate for alkaline phosphatase, used in combination with the oxidant NBT (nitro blue tetrazolium) to enhance blue color development.

Applications:
When alkaline phosphatase conjµgates are used for detection, BCIP is recommended for the following applications: Southern blotting, Western blotting, Northern blotting and dot/slot blots.

It is recommended to prepare a 50mg/mL stock solution in DMF.
Protocol for Preparation of BCIP/NBT Substrate Solution:
Add the following components
- 10mL of the Alkaline Phosphatase (AP) buffer (100mM Tris-HCl (pH9.5), 100mM NaCl and 10mM MgCl2)
- 33µL BCIP (50mg/mL in DMF)
- 44µL NBT (75mg/mL in 70% DMF).

NOTE: Avoid exposure to light - Prepare fresh developing solution and use within an hour - BCIP and NBT solutions in DMF do not freeze. They are stable for approximately 2 years when stored at -20°C in the dark.

More products around "BCIP, molecular biology grade"
Specifications Assay: min. 98% (HPLC) pH (5% in water): 1.0-2.0 Solubility (water, 20°C): 582... more
 

Technical Data:

Specifications
Assay: min. 98% (HPLC)
pH (5% in water): 1.0-2.0
Solubility (water, 20°C): 582 g/L
MP = 120°C
Chloride: max. 0.001%
Pb: max. 0.005%
Free acid: max. 0.1%
C8H6BrClNO4P x C7H9N
MW = 433.64 g/mol

Source

synthetic

Sicherheits Hinweise / Safety

H-Sätze: H315, H319, H335
GHS-Symbole: GHS07

Klassifizierungen / Classification

EC-Nr: 229-506-1
CAS-Nr: 6578-06-9
eclass-Nr: 32-16-04-05
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

Here you will find information and further literature on BCIP, molecular biology grade. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

 
 
 
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