Affinity Chromatography Purification

Affinity chromatography is a useful technique for separating proteins in a reversible interaction between the target protein and ligands bound to beads (affinity resin). The interaction can be biospecific, for example antibodies binding to protein A, or non-biospecific, for example histidine-tagged proteins binding to metal ions.

The chromatography technique provides high selectivity, high resolution and high capacity. High purity can often be achieved in a single step. Large sample volumes can be handled and samples can be applied under conditions that favor specific binding to the ligand. Elution is often performed under gentle conditions which helps preserve bioactivity. The target protein is eluted in a purified and concentrated form by modification of pH, ionic strength, or by introducing a competitive ligand.

Co-NTA Agarose for His-tagged protein purification > - Co-NTA MagBeads His-tagged protein purification > - Disposable columns 0.5mL > and 20mL > - Ni-IDA Agarose for His-tagged protein purification > - Ni-NTA-Agarose for His-tagged protein purification > - Ni-IDA MagBeads His-tagged protein purification > - Ni-NTA MagBeads His-tagged protein purification > - prepacked Ni-IDA Agarose columns

Affinity chromatography is a useful technique for separating proteins in a reversible interaction between the target protein and ligands bound to beads (affinity resin). The interaction can be... read more »
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Affinity Chromatography Purification

Affinity chromatography is a useful technique for separating proteins in a reversible interaction between the target protein and ligands bound to beads (affinity resin). The interaction can be biospecific, for example antibodies binding to protein A, or non-biospecific, for example histidine-tagged proteins binding to metal ions.

The chromatography technique provides high selectivity, high resolution and high capacity. High purity can often be achieved in a single step. Large sample volumes can be handled and samples can be applied under conditions that favor specific binding to the ligand. Elution is often performed under gentle conditions which helps preserve bioactivity. The target protein is eluted in a purified and concentrated form by modification of pH, ionic strength, or by introducing a competitive ligand.

Co-NTA Agarose for His-tagged protein purification > - Co-NTA MagBeads His-tagged protein purification > - Disposable columns 0.5mL > and 20mL > - Ni-IDA Agarose for His-tagged protein purification > - Ni-NTA-Agarose for His-tagged protein purification > - Ni-IDA MagBeads His-tagged protein purification > - Ni-NTA MagBeads His-tagged protein purification > - prepacked Ni-IDA Agarose columns

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Ni-IDA MagBeads for His-tagged protein purification Ni-IDA MagBeads for His-tagged protein...
Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments...
From €60.69 *
Ni-NTA MagBeads Ni-NTA MagBeads for His-tagged protein...
Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments...
From €87.38 *
Co-NTA MagBeads for His-tagged protein purification Co-NTA MagBeads for His-tagged protein...
Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments...
From €97.09 *
Ni-IDA, Co-IDA Agarose Ni-IDA Agarose for His-tagged proteins
Nickel-loaded agaroses are the most frequently used products for the efficient purification of 6×histidine-tagged recombinant fusion proteins. Because Ni2+ ions show high affinity for 6×histidine-tagged fusion proteins, extremely high...
From €111.30 *
Ni-IDA/Ni-NTA-Agarose Testkit (2 x 1mL) Ni-IDA/Ni-NTA-Agarose Testkit (2 x 1mL)
Testkit for testing the Genaxxon Ni-IDA- and NTA Agarose. Testkit contains 1mL of each Agarosetype.
€20.98 *
Co-NTA-Agarose for His-tagged proteins Co-NTA-Agarose for His-tagged proteins
NTA-Agarose consists of the tetradentate chelating agent, nitrilotriacetic acid (NTA), covalently coupled agarose beads, and is loaded with Co2+ ions. In processing recombinant fusion proteins from eukaryotes or in the case of special...
From €194.17 *
Pre-Packed Ni-IDA Agarose columns Pre-Packed Ni-IDA Agarose columns
Genaxxon bioscience offers pre-packed Ni2+ IDA columns in ready-to-use format for His-tag protein purification processes by gravity flow. Fast purifications with good yield of target proteins are obtainable by this method. Both matrices...
From €302.96 *
Mini spin columns for affinity purification Empty Spin Columns 0,5mL for Affinity...
Empty Spin Columns for standard protein purification procedures, e.g. affinity chromatography with Ni-IDA, Ni-NTA, Co-IDA oder Co-NTA agaroses. The smallest column size does have a capacity of 0.5mL (S) and are ideal for protein...
From €133.71 *
empty midi spin column for affinity purification Empty Spin Columns 20 mL for Affinity...
Empty Spin Columns for standard protein purification procedures, e.g. affinity chromatography with Ni-IDA, Ni-NTA, Co-IDA oder Co-NTA agaroses. The smallest column size does have a capacity of 0.5mL (S) and are ideal for protein...
From €60.78 *
MDYKDHDG-DYKDHDI-DYKDDDDK-OH Peptide MDYKDHDG-DYKDHDI-DYKDDDDK-OH Peptide
DYKDHDG-DYKDHDI-DYKDDDDK, a synthetic peptide was specifically designed for immunoaffinity chromatography. It allows elution under mild and non-denaturing conditions. Several antibodies against this peptide have been developed. The...
€397.84 *
3x FLAG Peptide - DYKDDDDK-DYKDDDDK-MDYKDDDDK 3x FLAG Peptide - DYKDDDDK-DYKDDDDK-MDYKDDDDK
The DYKDDDDK peptide was developed specifically for immunoaffinity chromatography. The peptide allows the competitive elution of proteins (amino terminal, metaminoterminal or carboxy-terminal) FLAG® fusion proteins from anti-FLAG M1 or...
From €143.20 * €160.00 *
Ni-NTA Agarose für His-tagged Proteine Ni-NTA Agarose für His-tagged Proteine
Ni-NTA-Agarose wurde für die Affinitätsreinigung von Proteinen entwickelt, die eine Polyhistidin-Markierung tragen. Diese Affinitätschromatographie-Matrix basiert auf BioWorks-Workbeads, die aus 7,5% vernetzter Agarose bestehen. Das...
From €22.05 *
FLAG-Peptide - DYKDDDDK FLAG-Peptide - DYKDDDDK
The DYKDDDDK peptide was specifically designed for immunoaffinity chromatography. It allows elution under mild and non-denaturing conditions. Several antibodies against this peptide have been developed. Usual working concentration is...
From €135.00 *