Affinity Chromatography Purification

Affinity chromatography is a useful technique for separating proteins in a reversible interaction between the target protein and ligands bound to beads (affinity resin). The interaction can be biospecific, for example antibodies binding to protein A, or non-biospecific, for example histidine-tagged proteins binding to metal ions.

The chromatography technique provides high selectivity, high resolution and high capacity. High purity can often be achieved in a single step. Large sample volumes can be handled and samples can be applied under conditions that favor specific binding to the ligand. Elution is often performed under gentle conditions which helps preserve bioactivity. The target protein is eluted in a purified and concentrated form by modification of pH, ionic strength, or by introducing a competitive ligand.

Co-IDA Agarose for His-tagged protein purification - Co-NTA Agarose for His-tagged protein purification - Co-IDA MagBeads His-tagged protein purification - Co-NTA MagBeads His-tagged protein purification - Disposable columns  0.6mL and 20mL - Ni-IDA Agarose for His-tagged protein purification - Ni-NTA-Agarose for His-tagged protein purification - Ni-IDA MagBeads His-tagged protein purification - Ni-NTA MagBeads His-tagged protein purification - prepacked Co-IDA Agarose columns - prepacked Ni-IDA Agarose columns - Workbeads Protein A