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HiDiTaq DNA polymerase for better Sake taste and flavor: HiDiTaq for qPCR for the unambiguous identification of point mutations in yeast!
Read more about a recent case report from Japan.

 

Point mutations are the cause of many hereditary diseases and are considered risk factors, for example, for Alzheimer's Disease.
One of the main causes of many hereditary diseases could not be resolved precisely enough by mid-2016, even with the "super tool" CRISPR / Cas9: the point mutation.

 

Sustainability at Genaxxon

We at Genaxxon bioscience are aware of our corporate responsibility. Our philosophy is to practice sustainability whenever possible. The following are some examples of the many changes that we have implemented successfully.

 

Nowadays there are many different suppliers and systems for polymerase chain reaction (PCR) and of its derivative techniques, quantitative, or real-time, PCR (qPCR) and reverse transcription PCR (RT-PCR). Competition gives you the opportunity to simplify diagnostics and to make research more financially favorable.

 

We are proud to inform you that Genaxxon bioscience has been successfully certified according TÜV ISO 9001: 2015!

 

PCR Beads Ready-To-Use lyophilized - LyoBeads:

Get the same reliable performance in your realtime PCR as before, now with a two-year shelf life at room temperature!

Pre-formulated, freeze-dried realtime PCR master mix in bead format. No need to store the PCR Master Mix (Beads) in the refrigerator or freezer. No unnecessary freeze-thaw cycles. No wasted time for thawing the master mix each time!

 

The qPCR and Digital PCR Conference started with lectures on the reliability of qPCR and ddPCR, from their technical limitations to the interpretation of results to errors in published data with a very interesting presentation by Prof. Stephen Bustin.

 

miRNAs in Gene Expression

miRNAs occur naturally in animal and plant organisms. They are highly conserved and play an important role in gene regulation, especially in gene silencing. They have an important function in cell differentiation and dedifferentiation, for example in female fertility and preimplantation development.

 

Digital PCR (dPCR)

Doing digital PCR means to carry out a PCR reaction with only one single amplification step nonetheless resulting in absolute/quantitative values. For dPCR, in contrast to conventional real-time PCR (qPCR), no standard curves, calculating of Ct-values or standard references are required because the single amplification step is performed to obtain a pure yes (positive) or no (negative) answer.

 

Citations in Scientific Literature

In the articles below you will find the authors already relying on the Genaxxon products. We say thank you to these authors, trusting in our service. Let us convince you as well.

 

By using the new HotScriptase 2X Cell master mix from Genaxxon RNA viruses (e.g. Zika virus, dengue virus, yellow fever virus) can be detected directly from body fluids as blood, salvia or semen without isolating or purifying RNA prior to RT-PCR.

 

Using Genaxxon's realtime PCR master mixes and DNA purification kits a PCR and Genetic Engineering workshop for 28 Researchers at the University of Tehran was held by Dr. Behdad Delavari from Yekta Tejarat Akam. The workshop and training course was conducted by high level teachers from the Universit of Tehran who used products from Genaxxon for the practical part of the course.

 

SNP - Allel Specific Discrimination

There's no accounting for taste.
Cilantro (Coriander): some people love it in their food, some hate it. Here we are detecting a genomic SNP (rs72921001) in HeLa genomic DNA. This SNP is reported to be close to a number of genes coding for olfactory receptors. (Reference: Eriksson N. et al. (2012), "A genetic variant near olfactory receptor genes influences cilantro preference.")

Considering, that only the C-allele specific primer is extended and yielding in a specific amplicon, we can conclude a genetic predisposition in disliking cilantro, as this SNP is significantly associated with detecting a soapy taste to cilantro.

 

CRISPR/Cas9 optimized workflow

The gRNA ("guide RNA") of the CRISPR/Cas9 system is a short synthetic RNA composed of a sequence (tracrRNA = non-target specific sequence) necessary to bind to the Cas9 protein and a gene specific ca. 20 nucleotide “spacer” or “targeting” sequence (crRNA) which defines the genomic target to be modified. We show in a scheme (according to Liang et al., 2015) an optimized workflow for cell analyzes with the CRISPR/Cas9 system from the theoretical design of the gRNA via transcription and transfection to the analysis of the experimentally obtained data within shortest time with special tips for products to use.

 

CRISPR/Cas9 Methods

Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) systems are revolutionizing the field of genome editing providing a powerful tool for disrupting the gene of interest. Able to achieve highly flexible and specific targeting, CRISPR-Cas systems can be modified and redirected being a powerful tool for genome editing in many species, including mammalian cells.

 

Basics of quantitative RT-PCR

Real-time RT-PCR compared to end-point detection methods.
Description of different methods for gene expression quantitation by real-time RT-PCR and end-point detection/quantification techniques.

 

Transfection of Fibroblasts with GenaxxoFect

Genaxxon´s transfection media are ideally suited for the transfection of fibroblast cell lines. Tests have proven that in particular GenaxxoFect represents the best suitable reagent for this cell lines.

 

GenaxxoFect siRNA enables trypsinization of transfected cells, even when the transfection was more than 24 hours ago.

 

results of our survey in 2015

Results of our online survey

We would like to thank our customers for the great response in answering our online survey and the excellent ratings for our service and our quality and price-performance ratio.

 

Our new
HotScriptase RT Cell Mastermix:
For the cDNA synthesis directly from cells, there is no need for a time-consuming and expensive cell lysis or RNA purification any more.

 

cDNA synthesis: risk of contamination will be reduced

Every single pipetting step and each tube that has to be opened increases the risk of contamination. So far, reverse transcription involved the risk of contaminating the valuable sample by many pipetting steps. With our new all-in-one enzyme > HotScriptase RT Polymerase the risk of contamination is clearly minimized with the zero-step protocol. Moreover, not to mention the considerable time saving.

 

RNA and DNA Purification in 96-well Format - New at Genaxxon

The new Genaxxon PurePro 96-well RNA and DNA Purification Kits enable high-yield, high-purity, RNA and DNA extractions from a wide variety of sample types in a high-throughput platform. These kits allow RNA and DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, using a 96-well plate format.

 

quantitative RT-PCR (engl.)

Genaxxon bioscience offers new an innovative "one-step" method to transcribe mRNA into DNA with simultaneous quantification of DNA. With the new HotScriptase enzyme and the HotScriptase master mixes the RNA has only to be added to the PCR reaction and the PCR program simply has to be started.

 

Protocols for transfection techniques vary widely but do most often not include trypsinization protocols of cells, transfection protocols of neuronal cells or the transfection of suspension cells: Our Genaxxon bioscience team is constantly endeavoured to bring our products and findings up to date. Here we will share our current experiences and findings for transfection, in particular to our GennaxoFect products, with you. We would be pleased if you would inform us of your observations about our GenaxxonFect products to share them with other customers on this platform.

 

MHC-I and MHC-II Peptides

Genaxxon bioscience offers different Peptides for examination on binding studies of MHC-Class-I and MHC-Class-II complexes. The advantage of the Genaxxon bioscience peptides is their defined and known amino acid sequence and the high purity, which enables an exact interpretation of results, without ambiguity that might be caused by impurities.