Collagenase Type I (EC 3.4.24.3)

Collagenase Type I (EC 3.4.24.3)


Order number: C4255.0100

Shipping: shipped at RT, store at +2°C to +8°C
CAS-Nr: 9001-12-1

Ready to ship today,
Delivery time appr. 1-3 workdays

€99.52 *

Weight:

Please select the favoured pack size.

Genaxxon Collagenase from Clostridium histolyticum is an enzymatic mixture from Collagenase,... more
Product information "Collagenase Type I (EC 3.4.24.3)"

Genaxxon Collagenase from Clostridium histolyticum is an enzymatic mixture from Collagenase, Clostripain (cleaves peptides preferentially at the carboxyl side of arginine residues), several tryptic (acts preferentially at the carboxyl side of arginine and lysine residues) and chymotryptic activities (non-specific). Type I Collagenase is recommended for cell preparation from epithelial, liver, lung tissue, tissue of the suprarenal gland and adipose tissue. The specific activity is 125 to 250 Mandl-Units per mg dry substance.

Clostridium histolyticum collagenase is an enzyme mixture of collagenase, clostripain and tryptic and proteolytic activities. Collagenase type IV has low tryptic, high collagenase and normal clostripain activity. Type I Collagenase is recommended for cell preparation from epithelial, liver, lung tissue, tissue of the suprarenal gland and adipose tissue. The specific activity 100 to 250 Mandl units per mg of dry matter.

Collagenase is produced by two separate and distinct genes in Clostridium histolyticum. Both genes have been cloned and sequenced (Yoshihara 1994). The colG gene codes for type I collagenase, a 936 amino acid protein, while the colH gene codes for type II collagenase, a 1021 amino acid protein. Both genes share 72% identity, the proteins only 43%. Both gene products can be present as two or more isoforms differing in molecular weight. Therefore collagenase mixtures can contain six to eight different proteins in a molecular weight range from 68 to 130 kDa. Substrate 
specificity studies have demonstrated that the colG gene prefers natural substrates such as intact collagen, compared to the colH gene product which preferentially digests short synthetic substrates (FALGPA) (Eckhard et al. 2009 and Matsushita 1999).

General description:
The treatment of tissue with collagenase causes the careful, selective degradation of the intercellular matrix, and does not affect the growth of the cells. The collagenase offered by Genaxxon bioscience is a mixture of different proteolytically active enzymes and requires calcium ions for both the catalytic activity and the binding to the collagen molecule. In contrast to vertebrate collagenase, Clostridium histolyticum collagenase digests native triple helix collagen into small peptides, which is the major use of collagenases in cell culture application.

For optimal results, a well balanced mixture of proteolytic enzymes is necessary. Four different collagenases, Collagenase Type I,  Collagenase Type IICollagenase Type III and Collagenase Type IV, are available for this. Type IV is usually used together with other enzymes, such as trypsin, elastase or hyaluronidaseTrypsin or Trypsin/EDTA conventionally used in the cell culture degrades the matrix only slowly and can cause irreversible damage to the released cells. Therefore, we recommend Accutase instead of trypsin or trypsin/EDTA.

Clostridium collagenases belongs to the metalloproteases, a large family of proteases that shares a zinc-containing motif at the center of the active site (Gonzales and Robert-Baudouy 1996). The enzyme is reversibly inactivated at high pH values and irreversibly inactivated at low pH values.

Inhibitors of collagenase include cysteine, EDTA, o-phenanthroline, 8-hydroxyquinoline-5-sulfonate, bipyridyl, 2,3-dimercaptopropanol or Hg2+, Pb2+, Cd2+, Cu2+. Collagenase is NOT inhibited by diisopropylphosphorofluoridate (DFP) or serum.

Lyphilised Collagenase should be stored at +2°C to +8°C and remain stable without loss of activity for at least three years. Enzyme should be protected from moisture. Dissolved Collagenase can be aliquoted and stored at -20°C for one year.

Conversion rates of collagenase activity units: 1 PZ U/mg ~ 3.9 FALGPA U/mg - 1 PZ U/mg ~ 1000 Mandl or CDU U/mg - 1 PZ U/mg ~ 10 HP U/mg (PZ-units according to Wünsch).

More products around "Collagenase Type I (EC 3.4.24.3)"
Our comment on "Collagenase Type I (EC 3.4.24.3)"
Shows average amounts of collagenase, caseinase, clostripain, and tryptic activities.
Specifications: Specific activity: >125 units/mg dry weight. Shows average amounts of... more
 

Technical Data:

Specifications:
Specific activity: >125 units/mg dry weight.
Shows average amounts of collagenase, caseinase, clostripain, and tryptic activities.
It is generally recommended for fat, adrenal, and liver cells.

application:

Recommended for cell preparation from fat, adrenal, and liver cells.

Source

Clostridium histolyticum

Sicherheits Hinweise / Safety

H-Sätze: H317, H334
GHS-Symbole: GHS08

Klassifizierungen / Classification

EC-Nr: 232-582-9
CAS-Nr: 9001-12-1
eclass-Nr: 32-16-04-10
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

Hier finden Sie Infos und weiterführende Literatur zu Collagenase Type I (EC 3.4.24.3)

 
 
 
Read, write and discuss reviews... more
Customer evaluation for "Collagenase Type I (EC 3.4.24.3)"
Write an evaluation
Evaluations will be activated after verification.
Please enter these characters in the following text field.

The fields marked with * are required.

Genaxxon PCR Mastermix PCR Master Mix (2X)
From €55.00 *
Hint!
X-Gal / 5-Bromo-4-chloro-3-indolyl-b-D-galactopyranoside X-Gal /...
From €36.80 *
Hint!
GreenMasterMix (2X) Low ROX for qPCR GreenMasterMix (2X) Low ROX for qPCR
From €20.00 * €75.00 *