Collagenase Type IV (EC 3.4.24.3)

Collagenase Type IV (EC 3.4.24.3)


Order number: C4310.0100

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CAS-Nr: 9001-12-1

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Clostridium histolyticum collagenase is an enzyme mixture of collagenase, clostripain and... more
Product information "Collagenase Type IV (EC 3.4.24.3)"

Clostridium histolyticum collagenase is an enzyme mixture of collagenase, clostripain and tryptic and proteolytic activities. Collagenase type IV has low tryptic, high collagenase and normal clostripain activity. Type IV collagenase is recommended for the isolation of Langerhans islet cells and has a specific activity of 100 to 250 Mandl units per mg of dry matter.

Collagenase is produced by two separate and distinct genes in Clostridium histolyticum. Both genes have been cloned and sequenced (Yoshihara 1994). The colG gene codes for type I collagenase, a 936 amino acid protein, while the colH gene codes for type II collagenase, a 1021 amino acid protein. Both genes share 72% identity, the proteins only 43%. Both gene products can be present as two or more isoforms differing in molecular weight. Therefore collagenase mixtures can contain six to eight different proteins in a molecular weight range from 68 to 130 kDa. Substrate 
specificity studies have demonstrated that the colG gene prefers natural substrates such as intact collagen, compared to the colH gene product which preferentially digests short synthetic substrates (FALGPA) (Eckhard et al. 2009 and Matsushita 1999).

General description:
The treatment of tissue with collagenase causes the careful, selective degradation of the intercellular matrix, and does not affect the growth of the cells. The collagenase offered by Genaxxon bioscience is a mixture of different proteolytically active enzymes and requires calcium ions for both the catalytic activity and the binding to the collagen molecule. In contrast to vertebrate collagenase, Clostridium histolyticum collagenase digests native triple helix collagen into small peptides, which is the major use of collagenases in cell culture application.

For optimal results, a well balanced mixture of proteolytic enzymes is necessary. Four different collagenases, Collagenase Type ICollagenase Type II, Collagenase Type III and Collagenase Type IV, are available for this. Type IV is usually used together with other enzymes, such as trypsin, elastase or hyaluronidase. Trypsin or Trypsin/EDTA conventionally used in the cell culture degrades the matrix only slowly and can cause irreversible damage to the released cells. Therefore, we recommend Accutase instead of trypsin or trypsin/EDTA.

Clostridium collagenases belongs to the metalloproteases, a large family of proteases that shares a zinc-containing motif at the center of the active site (Gonzales and Robert-Baudouy 1996). The enzyme is reversibly inactivated at high pH values and irreversibly inactivated at low pH values.

Inhibitors of collagenase include cysteine, EDTA, o-phenanthroline, 8-hydroxyquinoline-5-sulfonate, bipyridyl, 2,3-dimercaptopropanol or Hg2+, Pb2+, Cd2+, Cu2+. Collagenase is NOT inhibited by diisopropylphosphorofluoridate (DFP) or serum.

Lyphilised Collagenase should be stored at +2°C to +8°C and remain stable without loss of activity for at least three years. Enzyme should be protected from moisture. Dissolved Collagenase can be aliquoted and stored at -20°C for one year.

Conversion rates of collagenase activity units: 1 PZ U/mg ~ 3.9 FALGPA U/mg - 1 PZ U/mg ~ 1000 Mandl or CDU U/mg - 1 PZ U/mg ~ 10 HP U/mg (PZ-units according to Wünsch).

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Our comment on "Collagenase Type IV (EC 3.4.24.3)"
Low tryptic, high Collagenase and normal Clostripain activity.
Specifications: Specific activity: >125 units/mg dry weight.  Low tryptic, high... more
 

Technical Data:

Specifications:
Specific activity: >125 units/mg dry weight. 
Low tryptic, high Collagenase and normal Clostripain activity.

application:

Commonly used for islets and other applications where receptor integrity is crucial.

Source

Clostridium histolyticum

Sicherheits Hinweise / Safety

H-Sätze: H317, H334
GHS-Symbole: GHS08

Klassifizierungen / Classification

EC-Nr: 232-582-9
CAS-Nr: 9001-12-1
eclass-Nr: 32-16-04-10
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

Hier finden Sie Infos und weiterführende Literatur zu Collagenase Type IV (EC 3.4.24.3)

 
 
 
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Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.

Quelle/Source: NCBI PubMed >

Stabilin-1 is expressed in human breast cancer and supports tumor growth in mammary adenocarcinoma mouse model

Vladimir Riabov, Shuiping Yin, Bin Song, Aida Avdic, Kai Schledzewski, Ilja Ovsiy, Alexei Gratchev, Maria Llopis Verdiell, Carsten Sticht, Christina Schmuttermaier, Hiltrud Schönhaber, Christel Weiss, Alan P. Fields, Katja Simon-Keller, Frederick Pfister, Sebastian Berlit, Alexander Marx, Bernd Arnold, Sergij Goerdt, Julia Kzhyshkowska

Oncotarget. 2016 May 24; 7(21): 31097–31110. Published online 2016 Apr 20. doi: 10.18632/oncotarget.8857

PMCID: PMC5058742

 
 
 
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