5/6-TAMRA (5/6-carboxytetramethylrhodamine) - mixed isomers



Order number: S5410.0100

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CAS-Nr: 98181-63-6

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5/6-TAMRA is a mixtures of the 5' and 6' isomers of carboxytetramethylrhodamine (TMR) free acid.... more
Product information "5/6-TAMRA (5/6-carboxytetramethylrhodamine) - mixed isomers"

5/6-TAMRA is a mixtures of the 5' and 6' isomers of carboxytetramethylrhodamine (TMR) free acid. The non-activated single isomer carboxytetramethyl rhodamine derivative is used as a red fluorescent derivatization reagent for covalently labeling proteins and alkylamino modified nucleic acids. The fluorescence quantum yield of TAMRA conjugates is about one-fourth that of fluorescein conjugates. Nevertheless, TAMRA conjugates often appear brighter than the corresponding fluorescein conjugates due to the fact that TAMRA is readily excited by the 546nm spectral line from mercury-arc lamps used in most fluorescence microscopes and is intrinsically more photostable than fluorescein. TAMRA is also efficiently excited by the 543nm spectral line of the green He-Ne laser.

Succinimidyl esters of 5-TAMRA >, 6-TAMRA > or of the mixed 5/6 TAMRA isomers > are the labeling reagents of choice for the preparation of fluorescent bioconjugates, including peptide, protein, nucleotide and nucleic acid conjugates, especially for fluorescent antibodies or avidine derivatives used in immunochemistry. These fluorescent dyes are also used as acceptors for FAM > fluorophores in FRET studies.

More Fluorescent Dyes from Genaxxon: 5/6-TAMRA >, 5-TAMRA >, FAM >, ROX >, DAF-2 >.

More products around "5/6-TAMRA (5/6-carboxytetramethylrhodamine) - mixed isomers"
Specifications: Assay: min. 90% (HPLC) Appearance: dark green powder Identity: determined by... more
 

Technical Data:

Specifications:
Assay: min. 90% (HPLC)
Appearance: dark green powder
Identity: determined by NMR/IR
Fluorescence: λex 543 nm; λem 575 nm in 0.1M Tris pH8.0
Soluble in: methanol, DMSO, DMF or aqueous buffers (pH ≥6.5)
C25H22N2O5
MW = 430.45 g/mol

application:

fluorescent dye for labelling of proteins

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

CAS-Nr: 98181-63-6
eclass-Nr: 39-21-01-90
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

Here you will find information and further literature on 5/6-TAMRA (5/6-carboxytetramethylrhodamine) - mixed isomers. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

 
 
 
Literatur .... more

Referenzen..

Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.

Quelle/Source: NCBI PubMed >

Leptin Enhances Availability of Apoptotic Cell-Derived Self-Antigen in Systemic Lupus Erythematosus

Gil Amarilyo, Noriko Iikuni, Aijing Liu, Giuseppe Matarese, Antonio La Cava

PLoS One. 2014; 9(11): e112826. Published online 2014 Nov 17. doi: 10.1371/journal.pone.0112826

PMCID: PMC4234630

 
 
 
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