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6-TAMRA is the pure 6-isomer of carboxytetramethylrhodamine (TMR) free acid. The non-activated single isomer carboxytetramethyl rhodamine derivative is used as a red fluorescent derivatization reagent for covalently labeling proteins and alkylamino modified nucleic acids. The fluorescence quantum yield of TAMRA conjugates is about one-fourth that of fluorescein conjugates. Nevertheless, TAMRA conjugates often appear brighter than the corresponding fluorescein conjugates due to the fact that TAMRA is readily excited by the 546nm spectral line from mercury-arc lamps used in most fluorescence microscopes and is intrinsically more photostable than fluorescein. TAMRA is also efficiently excited by the 543nm spectral line of the green He-Ne laser.
Succinimidyl esters of 5-TAMRA >, 6-TAMRA > or of the mixed 5/6 TAMRA isomers > are the labeling reagents of choice for the preparation of fluorescent bioconjugates, including peptide, protein, nucleotide and nucleic acid conjugates, especially for fluorescent antibodies or avidine derivatives used in immunochemistry. These fluorescent dyes are also used as acceptors for FAM > fluorophores in FRET studies.
Assay: min. 90% (HPLC)
Identity: determined by NMR/IR
Fluorescence: λex 543 nm; λem 575 nm in 0.1M Tris pH8.0
Soluble in: methanol, DMSO, DMF or aqueous buffers (pH ≥6.5)
MW = 430.45 g/mol
application:fluorescent dye for labelling of proteins
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationCAS-Nr: 91809-67-5
Dokumente - Protokolle - Downloads
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Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
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Gil Amarilyo, Noriko Iikuni, Aijing Liu, Giuseppe Matarese, Antonio La Cava
PLoS One. 2014; 9(11): e112826. Published online 2014 Nov 17. doi: 10.1371/journal.pone.0112826