Agarose LM - low melting



Order number: QM3049.0010

Shipping: shipped and stored at RT
CAS-Nr: 9012-36-6

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Agarose LM is the original low melting agarose. This "molecular biology grade" agarose gives... more
Product information "Agarose LM - low melting"

Agarose LM is the original low melting agarose. This "molecular biology grade" agarose gives gels with better properties and higher transparency than the standard normal melting point agarose. The agarose is equivalent to SeaPlaque™ from Lonza. Agarose LM is a low melting agarose with a very high separation capacity for large DNA fragments (>1000 bp), RNA and proteins. Enzymatic manipulations (eg, digestion, ligation, PCR, etc.) can be performed in the molten gel. Extraction of the DNA is therefore not necessary. Low melting Agarose LM is excellently suited for digestion with β-agarase (S5223) >. Large DNA fragments can be isolated very gently. The low melting agarose LM is used in the analytical and preparative field. For optimal results, the gel should be stored for at least 1 hour before use at + 2 ° C to + 8 ° C.

Genaxxon offers agaroses for a wide variety of applications. There are agaroses with different melting points or with normal or high-resolution separation properties of DNA fragments.

Standard melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use.

Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase (S5223) >, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel.

High resolution agarose (normal melting or low melting) for high resolution elektrophoresis
to differentiate DNA fragments with only 2 bp size difference: M3046 (Agarose Tiny (low melt, high resolution comparable to NuSieve®) >M3048 Agarose Tiny LMH > (normal melting point, high resolution) or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®).

For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye GelRed® (M3199) > is recommended.

Test sample available! No shipping costs within Germany.

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Our comment on "Agarose LM - low melting"
- low melting and gelling temperature - high gel transparancy for very clear gels - the ideal agarose for isolation of nucleic acid fragments or proteins after electrophoretic separation. - applicable for enzymatic reactions in liquid agarose (in gel applications) - highly suitable for the immobilization of micro organisms and for virus plaque assays - suitable for cell and tissue culture (animal and plant cells)
Specifications: Electroendosmosis (EEO): <0.14 Sulfate: <0.11% Loss on drying: max. 10%... more
 

Technical Data:

Specifications:
Electroendosmosis (EEO): <0.14
Sulfate: <0.11%
Loss on drying: max. 10%
Gel strengh (1.5%): >200 g/cm2
Gelling temperature (1.5%): <30°C
Melting temperature (1.5%): <65°C
pH7.5 (1% in water)
DNAse and RNAse: not detected

application:

- for electrophoresis of DNA, RNA and proteins. - for enzymatic reaction in liquid agarose (in gel applications) - for immobilization of micro organisms or virus plaque assays

Source

seaweed

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

EC-Nr: 232-731-8
CAS-Nr: 9012-36-6
eclass-Nr: 32-16-04-03
Dokumente - Protokolle - Downloads more


Dokumente - Protokolle - Downloads

Here you will find information and further literature on Agarose LM - low melting. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

 
 
 
Literatur .... more

Referenzen..

Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.

Quelle/Source: NCBI PubMed >

An advanced glioma cell invasion assay based on organotypic brain slice cultures

Tanja Eisemann, Barbara Costa, Jens Strelau, Michel Mittelbronn, Peter Angel, Heike Peterziel

BMC Cancer. 2018; 18: 103. Published online 2018 Jan 30. doi: 10.1186/s12885-018-4007-4

PMCID: PMC5789681