GreenMasterMix (2X) High ROX for qPCR

Realtime PCR Mastermix High ROX


Order number: M3052.0000

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GreenMastermix optimised for Real-Time PCR assays in Block systems that contains all components... more
Product information "GreenMasterMix (2X) High ROX for qPCR"

GreenMastermix optimised for Real-Time PCR assays in Block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains optimised amounts of all ingredients.

- HotStart Polymerase
- dATP, dCTP, dGTP, dTTP
- green fluorescent dye (an analogue fluorescent dye to SybrGreen that intercalates into DNA)
- ROX (500nM) as internal standard
- optimized reaction buffer with KCl and MgCl2
- stabilizers and enhancers) to enable even amplification of low copy number targets
- small aliquote size of 1.25mL

Stability at +2°C to +8°C (refrigerator): minimum of 8 months.

Our data show that this kit can perform comparable to ones from market leaders. This high ROX master mix is specially suited for the following instruments: ABi 5700, ABi 7300, 7700, 7900, 7900HT, ABi 7000 PRISM, ABi 7700 PRISM, Eppendorf Realplex 4, QuantStudio™, StepOne™ Plus.

Other realtime PCR master mixes from Genaxxon are: M3023 - GreenMastermix No ROX >, M3045 - ProbeMastermix No ROX >, M3011 - GreenMastermix Low ROX >, M3031 - ProbeMastermix Low ROX >, M3052 - GreenMastermix High ROX >, M3010 - ProbeMastermix High ROX >.

Test sample available! The test sample price will be refunded on the first official order of the product.

More products around "GreenMasterMix (2X) High ROX for qPCR"
Our comment on "GreenMasterMix (2X) High ROX for qPCR"
High Sensitivity and Specifity, Optimised buffer system, Reproducibility over a wide range of template amounts, Broad dynamic range. Aliquot size: 1.25mL. This master mix is stable for at least 8 months at +2°C to +8°C.
This GreenMastermix is a ready-to-use 2X mastermix containing 500nM ROX™ as passive Reference... more
 

Technical Data:

This GreenMastermix is a ready-to-use 2X mastermix containing 500nM ROX™ as passive Reference dye, and a green fluorescent dye.

Aliquot size: 1.25mL. This master mix is stable for at least 8 months at +2°C to +8°C.

application:

PCR Primer extension Multiplex PCR Low-copy targets PCR Real-time PCR Quantitative PCR

Unit Definition:

One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

Hier finden Sie Infos und weiterführende Literatur zu GreenMasterMix (2X) High ROX for qPCR

 
 
 
Literatur .... more

Referenzen..

Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.

Quelle/Source: NCBI PubMed >

MiR-744-5p inducing cell death by directly targeting HNRNPC and NFIX in ovarian cancer cells

Michael Kleemann, Helga Schneider, Kristian Unger, Philip Sander, E. Marion Schneider, Pamela Fischer-Posovszky, René Handrick, Kerstin Otte

Sci Rep. 2018; 8: 9020. Published online 2018 Jun 13. doi: 10.1038/s41598-018-27438-6

PMCID: PMC5998049

Investigation on tissue specific effects of pro-apoptotic micro RNAs revealed miR-147b as a potential biomarker in ovarian cancer prognosis

Michael Kleemann, Jeremias Bereuther, Simon Fischer, Kim Marquart, Simon Hänle, Kristian Unger, Verena Jendrossek, Christian U. Riedel, René Handrick, Kerstin Otte

Oncotarget. 2017 Mar 21; 8(12): 18773–18791. Published online 2016 Nov 4. doi: 10.18632/oncotarget.13095

PMCID: PMC5386646

miR-217-5p induces apoptosis by directly targeting PRKCI, BAG3, ITGAV and MAPK1 in colorectal cancer cells

Marion Flum, Michael Kleemann, Helga Schneider, Benjamin Weis, Simon Fischer, René Handrick, Kerstin Otte

J Cell Commun Signal. 2018 Jun; 12(2): 451–466. Published online 2017 Sep 14. doi: 10.1007/s12079-017-0410-x

PMCID: PMC5910322

 
 
 
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Customer evaluation for "GreenMasterMix (2X) High ROX for qPCR"
16 Sep 2016

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