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ProbeMastermix optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix without ROX is ready-to-use and contains optimised amounts of all ingredients.
- HotStart Polymerase
- dATP, dCTP, dGTP, dTTP
- optimized reaction buffer with KCl and MgCl2
- stabilizers and enhancers) to enable even amplification of low copy number targets
- small aliquote size of 1.25mL
Stability at +2°C to +8°C (refrigerator): minimum of 8 months.
Our data show that this kit can perform comparable to ones from market leaders.
Specially suited for Bio-Rad iCycler iQ, iQ5 and MyiQ™, CFX 96, CFX 480, Chromo 4™ Real-Time Detector, DNA Engine Opticon™, Mastercycler® ep realplex 2, Opticon® 2, Roche LightCycler® 96 and 480, Rotor-Gene™ / Corbet, QuantStudio™, Smart Cycler®.
Other realtime PCR master mixes from Genaxxon are: M3023 - GreenMastermix No ROX >, M3045 - ProbeMastermix No ROX >, M3011 - GreenMastermix Low ROX >, M3031 - ProbeMastermix Low ROX >, M3052 - GreenMastermix High ROX >, M3010 - ProbeMastermix High ROX >.
Test sample available! The test sample price will be refunded on the first official order of the product.
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High Sensitivity and Specifity, Optimised buffer system, Reproducibility over a wide range of template amounts, Broad dynamic range. Aliquot size: 1.25mL. This master mix is stable for at least 8 months at +2°C to +8°C.
This ProbeMastermix is a ready-to-use 2-time master mix that contains no ROX™
Aliquot size: 1.25mL. This master mix is stable for at least 8 months at +2°C to +8°C.
application:PCR Primer extension Multiplex PCR Low-copy targets PCR Real-time PCR Quantitative PCR
Unit Definition:One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads
Here you will find information and further literature on Probe Master Mix No ROX for qPCR. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.