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Quantitative PCR is an important tool for SNP and gene expression analysis. Two general fluorescent chemistries exist for quantitative detection of gene transcripts: probes (e.g. TaqMan®, Scorpions™ Probes, molecular beacons) and DNA-binding fluorescent dyes (e.g. ethidium bromide, SYBR® Green, EvaGreen®, PicoGreen®). Ampliqon offers the RealQ Plus 2x Master Mix in two formulations: for probe or with DNA-binding fluorescent dye, making them ideal for most quantitative PCR applications.
The RealQ Plus 2x Master Mixes are available with high, low or without ROX™ for optimal performance on most of the commonly used real-time PCR instruments. The RealQ Plus 2x Master Mixes promote high specificity and low background by using TEMPase Hot Start DNA Polymerase, a modified Taq DNA polymerase with hot start capabilities.
The RealQ Plus 2x Master Mix Green with high ROX™ is a singletube 2x reagent including all components necessary to perform DNA-binding dye based real-time DNA amplification. Just add primers and DNA. ROX™ internal reference dye level is optimized for the popular StepOne and StepOnePlus instruments from Life Technologies.
Detection limit: approximately 1 copy.
Quantitation limit: approximately 24 copies (0.08ng of human gDNA, correlating to 12 diploid genomes, with 2 gene copies per diploid genome)
Compatibility: Real-time instruments that require high ROX™ as internal reference dye e.g. StepOne and StepOnePlus from Life Technologies.
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- Gene Quantification
- Further products by Ampliqon A/S
- All-in-one optimized master mix, including green dye - High sensitivity - High efficiency and high specificity - Wide dynamic range - High reproducibility - Hot Start capacity for room temperature setup
The GreenMastermix is a ready-to-use 2-time mastermix that contains a green fluorescence dye, dUTP instead of dTTP and 500nM ROX™ (high ROX concentration) as reference dye.
application:Low-copy targets PCR Real-time PCR Quantitative PCR
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads
Here you will find information and further literature on RealQ Plus 2x Master Mix Green - High ROX. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: email@example.com or phone: +49 731 3608 123.