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Total RNA Purification and Extraction Kit with Antifoam Reagent >!
One-off special test sample price for our Total RNA Purification and Extraction Kit for high-quality RNA from tissue and cells. 50% discount on the list price of the smallest packaging unit! No shipping costs for shipments within Germany!
Genaxxon´s Total RNA Purification Mini Spin kit is designed for the rapid and efficient purification of high quality RNA from 1-30mg of tissue (fresh or frozen) and 10E4-10E7 cultured cells. The isolation protocol and buffer formulations were optimised for high isolation efficiency and purity of RNA. The Total RNA Purification Mini Spin kit utilises spin minicolumns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. The isolation procedure consists of 6 steps. In the first isolation step, the tissue is homogenized in order to disintegrate intercellular bonds (epithelial tissue) and fragmentize high-molecular proteins (muscle or connective tissue). Then the homogenate is lysed with guanidine thiocyanate and detergents. Any RNases are inactivated by guanidine thiocyanate and β-mercaptoethanol. The three-step washing stage effectively removes impurities and enzyme inhibitors.
Our RNA purification kits contain a novel antifoam reagent that prevents foaming:
- Improves the lysis
- Facilitates pipetting
- No filtration after lysis necessary
- Less material loss
The purified RNA is eluted using a low ionic strength buffer or RNase-free water and can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR and so forth.
Additional Genaxxon RNA purification kits and accessories: 96-well Plate Total RNA Purification Kit > and miRNA Purification Kit > or Total RNA Purification Mini Spin Kit PLUS > with ceramic beads for rapid cell disintegration.
You can reorder our RNA > and DNA purification columns > for the isolation of total RNA or DNA from enzymatic reactions or ceramic beads > separately. These articles can also be used with buffers/kits from other suppliers.
With our HotScriptase RT Polymerase >, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step. Our HotScriptase RT is also available as a mastermix >, with which you can directly amplify RNA directly from cells without prior isolation or purification of RNA!
Please check also our DNA purification kits >.
- Do you have any questions concerning this product?
- microRNA transfer
- Further products by Genaxxon bioscience
230µg RNA binding capacity on-column DNase I digestion for faster purification fast and efficient RNA purification
Purity of isolated RNA: A260/A280 = 1.7 - 1.9
Binding capacity of columns: approximately 230µg RNA
for up to 30mg frozen or preserved tissue
or for 10E4 - 10E7 cells from cell culture.
application:RNA purification for downstream applications as RT-PCR, RT-qPCR, Northern blotting, cDNA synthesis, primer extension, RT-PCR, RNA sequencing, micro arrays, etc.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-04-05
Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
The knockdown of the Mediator complex subunit MED15 restrains urothelial bladder cancer cells' malignancy
Isabella Syring, Richard Weiten, Tim Müller, Doris Schmidt, Susanne Steiner, Glen Kristiansen, Stefan C. Müller, Jörg Ellinger
Oncol Lett. 2018 Sep; 16(3): 3013–3021. Published online 2018 Jun 25. doi: 10.3892/ol.2018.9014
Isabella Syring, Niklas Klümper, Anne Offermann, Martin Braun, Mario Deng, Diana Boehm, Angela Queisser, Anne von Mässenhausen, Johannes Brägelmann, Wenzel Vogel, Doris Schmidt, Michael Majores, Anne Schindler, Glen Kristiansen, Stefan C. Müller, Jörg Ellinger, Zaki Shaikhibrahim, Sven Perner
Oncotarget. 2016 Apr 26; 7(17): 23043–23055. Published online 2016 Mar 30. doi: 10.18632/oncotarget.8469