Vorteile im Überblick
- Activity: 200 units/µL.
- Purity: Free of RNase activity resulting in an increase of full-length cDNA products.
- Purity: >98% protein homogeneity in SDS-PAGE electrophoresis.
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Produktinformationen "HH Scriptase III - Reverse Transkriptase"
HH Scriptase III reverse transcriptase is a genetically modified reverse transcriptase (RT) in which, compared to MMLV, RNase H activity and 3'-5' exonuclease activity have been deleted. Among other things, this results in a longer half-life and improved thermal stability of the enzyme at temperatures up to 55°C.
HH Scriptase III offers a higher cDNA yield, longer cDNAs and greater efficiency with GC-rich template RNAs, resulting in improved overall performance compared with wild-type MMLV enzymes and MMLV RNase H-minus enzymes.
Note:
HH Scriptase III reverse transcriptase offers
- improved thermal stability,
- improved processivity,
- higher yield and performance,
- suitability for RNA of sub-optimal purity or average integrity.
Tipp:
Genaxxon bioscience recommends testing Scriptase IV for RNA samples containing inhibitors. In various experiments, this enzyme has proved to be significantly more tolerant of a range of inhibitors.
Quality control:
| Nuclease activity | 50ng of radio labelled DNA or RNA is incubated with 200 units of the enzyme in 1X reaction buffer for one hour at 37°C, resulting in <1% release of free measurable radio activity in the supernatant. |
| Endonuclease Activity: | Incubation of 200 U of enzyme with 1μg λ DNA for 16 hours at 37°C resulted in no detectable degradation of the DNA as determined by gel electrophoresis. |
| Exonuclease Activity: | Incubation of 200 U of enzyme with 1 μg λ-Hind III digest DNA for 16 hours at 37°C resulted in no detectable degradation of the DNA as determined by gel electrophoresis. |
| Nickase Activity: | Incubation of 200 U of enzyme with 1 μg pBR322 for 16 hours at 37°C resulted in no detectable degradation of the DNA as determined by gel electrophoresis. |
| RNase Activity: | Incubation of 200 U of the enzyme with 1.6μg MS2 RNA for 16 hours at 37°C yields no degradation as determined agarose gel electrophoresis. |
| Purity: | >98% as determined by SDS-polyacrylamide gel analysis. The E.coli DNA content found by TaqMan qPCR is less than 1 copy if >200 U of the enzyme are used as DNA template in the qPCR assay. |
Specifications:
Activity: 200 units/µL.
Purity: Free of RNase activity resulting in an increase of full-length cDNA products.
Purity: >98% protein homogeneity in SDS-PAGE electrophoresis.
Enzyme is shipped together with 5X complete buffer.