Next Generation Sequencing - Library Preparation Solutions

NGS DNA Purification MagBeads bieten eine SPRI-basierte Chemie, die die Größenauswahl von Nukleinsäuren und die PCR-Aufreinigung für die Vorbereitung von DNA- und/oder RNA-Bibliotheken für die Sequenzierung der nächsten Generation beschleunigt und vereinfacht. Sie ist mit den gängigen Kits zur Vorbereitung von NGS-Bibliotheken kompatibel. Bei der Solid Phase Reversible Immobilization (SPRI)-Technologie werden paramagnetische Beads eingesetzt, um Nukleinsäuren wie DNA und/oder RNA selektiv nach Art und Größe zu binden. Sie müssen somit bei der DNA-Isolierung bzw. RNA-Extraktion nicht zentrifugieren oder aufwändige Filtrationsschritte durchführen. So ermöglicht SPRI sichere, hochleistungsfähige Protokolle für anspruchsvolle molekularbiologische Anwendungen wie qPCR, ddPCR, Sanger-Sequenzierung, NGS und Microarrays. NGS DNA Purification MagBeads sind mit den meisten Kits einsetzbar, die zur Herstellung von NGS-Bibliotheken (DNA und RNA) angeboten werden. Unser Kit ermöglicht den problemlosen Ersatz von Wettbewerberprodukten (Beads), z.B. AMPure XP Beads. In Kombination mit halbautomatischen und/oder automatisierten Gerätelösungen gelingt Ihnen die effiziente Aufreinigung selbst schwierigster Matrizes bei gleichzeitig reduzierter Fehlerrate und hoher Ausbeute. Wieso Reinigung und Qualitätskontrolle von DNA zur Anwendung bei der NGS?Der Reinigungsschritt ist in der Regel notwendig, um unerwünschtes Material zu entfernen, das die Sequenzierung stören könnte und zu unerwünschten Signalen führt. Einige NGS-Plattformen haben sehr strenge Anforderungen an die DNA-Fragmente, die sequenziert werden können. Das Abtrennen jeglicher DNA-Fragmente, die nicht im akzeptierten Größenbereich liegen ist dann für ein gutes Sequenzierergebnis unabdingbar.  Die optimale Bibliotheksgröße wird durch die Sequenzierungsanwendung vorgegeben. Es ist üblich, die Nukleinsäuren mit Hilfe von Magnetkügelchen zu reinigen. Die Qualitätskontrolle ist der letzte Prozess vor der Sequenzierung. Da die anschließenden Experimente zeitaufwändig und teuer sind, sind strenge Qualitätskontrollschritte erforderlich, um sicherzustellen, dass alle Proben für ihre Anwendungen geeignet sind. Die Bestätigung der Qualität und Quantität der DNA erhöht die Zuverlässigkeit der Sequenzierungsdaten! Das Protokoll unseres NGS MagBead DNA-Reinigungskit kann an die Vorgaben Ihrer aktuellen Liquid-Handling-Workstation (z. B. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent und Eppendorf) angepasst werden, wobei Ihr aktuelles Protokoll verwendet wird.

Genaxxon’s DNA Library Prep Kit for Illumina is your universal solution for preparing high-quality libraries for sequencing on Illumina high-throughput platforms. Designed to convert fragmented DNA input ranging from 1 ng to 1 μg into sequencing-ready libraries, this kit offers unmatched efficiency and reliability for your sequencing projects.WorkflowKey Features:Wide Compatibility: Ideal for various sample types, including low-quality FFPE and cfDNA, as well as conventional DNA samples.High Efficiency: Delivers superior library conversion rates, ensuring maximum yield from your input DNA.Accurate Detection: Enables precise identification of low-frequency mutations, making it perfect for sensitive sequencing applications.Streamlined Workflow: Experience a seamless, four-step workflow that completes in approximately three hours—all within a single reaction tube. This process eliminates the need for intermediate purification and sample transfers, reducing the risk of handling errors and preserving the integrity of your samples. With DNA Repair Enzymes II, the kit effectively repairs a wide range of DNA damages, including nicks, gaps, and oxidized bases, ensuring the highest quality libraries for your sequencing needs. Applications: Whole-genome sequencing Whole-exome or targeted capture sequencing Amplicon sequencing ChIP sequencing Metagenome sequencing Methylation sequencing Optimized for IlluminaThe resulting libraries are fully compatible with standard Illumina® sequencing reagents and protocols, delivering exceptional performance for high-coverage deep sequencing, de novo sequencing, and whole-genome resequencing projects.Compatibility: This kit is fully compatible with Genaxxon’s Unique Dual Index primer pairs. For 24 reactions, use the UDI MidiSet (M4402). For 96 reactions, you can either use 2x UDI Set A (M4403) or 1x UDI Set A (M4403) and 1x UDI Set B (M4404).

Genaxxon’s DNA Library Prep Kit PLUS for Illumina is an advanced solution designed to enhance your sequencing workflow on Illumina high-throughput platforms. This kit simplifies DNA library preparation by integrating DNA fragmentation, end repair, and dA-tailing into a single step. With adapter ligation, library amplification, and size selection performed directly—without additional purification—the entire process is completed in just 2 hours.WorkflowKey Features:Efficient Workflow: Streamline your process by performing fragmentation, end repair, and dA-tailing with a single enzyme mix, finishing in under 2 hours.Versatile Insert Sizes: Customize your insert sizes by adjusting fragmentation time, while minimizing inversions and chimeras for optimal results.Broad Compatibility: Designed for diverse DNA sources, with strict background bacterial control for accurate pathogen detection.Optimized Performance: The Genaxxon DNA Library Prep Kit PLUS is compatible with DNA from various sources, including challenging samples like FFPE DNA. Enhanced reagent optimization reduces false positives, improving sequencing yield and overall results. This kit is particularly well-suited for constructing PCR-free libraries using 100 ng of high-quality genomic DNA. Applications: Whole-genome sequencing Exon or other targeted sequencing Metagenomic sequencing Optimized for IlluminaGenaxxon’s DNA Library Prep Kit PLUS delivers exceptional performance for a range of sequencing applications, ensuring your libraries are fully compatible with Illumina® sequencing reagents and protocols, making it the ideal choice for efficient, high-quality sequencing preparation.Compatibility: This kit is fully compatible with Genaxxon’s Unique Dual Index primer pairs. For 24 reactions, use the UDI MidiSet (M4402). For 96 reactions, you can either use 2x UDI Set A (M4403) or 1x UDI Set A (M4403) and 1x UDI Set B (M4404).

High-Fidelity, Versatile, Ready-to-UseOverviewAchieve unparalleled accuracy and efficiency with Genaxxon’s NGSClone PCR Mastermix (2x). This high-fidelity, ready-to-use master mix features advanced hot-start technology and enhanced DNA polymerase for precise amplifications. Ideal for a wide range of templates, including GC-rich samples, it includes all essential components for reliable PCR results with minimal setup.Key FeaturesSuperior Fidelity and Processivity: Powered by a novel DNA polymerase, the NGSClone PCR Mastermix ensures precision with a remarkable 100-fold increase in fidelity compared to standard Taq polymerases. Experience accurate and efficient amplifications, thanks to enhanced extension speed and robust 3’-5’ exonuclease activity.Advanced Hot-Start Function: The antibody-mediated hot-start technology prevents non-specific amplifications by keeping the enzyme inactive at room temperature. This means fewer experimental inconsistencies and higher specificity.Versatile Performance: Whether you’re working with animal, plant, or cDNA templates, the NGSClone PCR Mastermix delivers high amplification efficiency across a diverse range of samples. It is especially effective with GC-rich templates, making it a versatile choice for a variety of PCR applications.Convenience and Ease-of-Use: Simplify your PCR workflow with this ready-to-use master mix. It comes complete with all essential components, requiring only the addition of primers and DNA to get started. Save time and reduce errors with a mix that’s ready to perform right out of the box.Elevate your PCR performance with Genaxxon’s NGSClone PCR Mastermix (2x) and experience the future of high-fidelity amplification.

Genaxxon’s FAST RNA-seq Library Preparation Kit is an all-in-one solution designed to deliver fast and efficient preparation of RNA sequencing libraries. Optimized for use with eukaryotic total RNA or purified mRNA samples from organisms such as animals, plants, and fungi, this kit offers unmatched flexibility with RNA input amounts ranging from 10 to 1000 ng. Whether you're working on high-throughput sequencing projects with MGI or Illumina platforms, this kit ensures high-quality library preparation with minimal effort.WorkflowEffortlessly prepare your RNA sequencing libraries in less time with an integrated workflow that reduces preparation time from 7 hours to just 3 hours.Key Features: Wide Compatibility: Ideal for a variety of eukaryotic samples, including total RNA and purified mRNA from animals, plants, and fungi. Flexible Input Range: Supports RNA input amounts from as low as 10 ng to as high as 1000 ng, providing versatility for different experimental setups. Streamlined Efficiency: Combines second-strand cDNA synthesis, dsDNA end repair, and dA-tailing into a single, time-saving step. Platform Versatility: Fully compatible with MGI and Illumina high-throughput sequencing platforms, ensuring flexibility across various sequencing needs. Buffer Options: Offers both non-strand-specific and strand-specific buffer options to suit varying experimental requirements. Streamlined Workflow: With a three-step process that simplifies second-strand cDNA synthesis, dsDNA end repair, and dA-tailing into one reaction, this kit saves time and minimizes errors associated with intermediate purifications. The workflow is optimized for speed and reliability, ensuring that high-quality libraries are produced efficiently. Applications: RNA-seq Library Preparation for MGI and Illumina sequencing platforms Total RNA and mRNA Library Preparation from a wide range of eukaryotic organisms Strand-Specific and Non-Strand-Specific RNA Library Preparation, providing flexibility based on experimental needsCompatibility This kit is fully compatible with Genaxxon’s Unique Dual Index primer pairs. For 24 reactions, use the UDI MidiSet (M4402). For 96 reactions, you can either use 2x UDI Set A (M4403) or 1x UDI Set A (M4403) and 1x UDI Set B (M4404). For optimal RNA enrichment, use the Poly (A) mRNA Capture Module with high-quality total RNA samples to avoid introducing a 3' bias during library preparation. For samples with low quality, we recommend using the rRNA Depletion Module to enhance RNA enrichment. In cases where RNA samples from plants or other eukaryotic cells show degradation but still exhibit 28S and 18S bands in agarose gel electrophoresis, increasing the total RNA input and adjusting PCR cycles accordingly can help achieve sufficient library yields.

Genaxxon’s 2X Oligo (dT)25 Capture Beads offer a reliable and efficient solution for isolating poly(A) RNA from purified total RNA samples. This capture method is based on the binding between the poly(A) tail at the 3' end of mRNA and the Oligo d(T) sequence on the magnetic microsphere. With a streamlined protocol, the capture process is completed in under 1 hour, making it ideal for fast and accurate RNA preparations. Key Features:High Specificity: Ensures targeted capture of poly(A) RNA from total RNA samples.Fast Protocol: Achieve RNA isolation in under 1 hour.Consistent Results: Every kit batch undergoes rigorous quality control and is verified through library preparation and sequencing, ensuring reliable and reproducible results.Complete Kit: Includes all required reagents, optimized for poly(A) RNA isolation.Applications: RNA purification for downstream applications such as RNA sequencing, RT-PCR, and gene expression analysis. Poly(A) RNA enrichment from total RNA samples for high-throughput sequencing.Seamless Compatibility: Fully compatible with Genaxxon’s FAST RNA-seq Library Prep Kit, offering an integrated workflow for RNA capture and library preparation.

Genaxxon’s rRNA Depletion Module offers a precise and efficient solution for removing ribosomal RNA (rRNA) from total RNA samples, enhancing the quality of RNA for downstream applications such as sequencing and transcriptome analysis. Tailored for prokaryotic RNA, this module effectively targets and depletes rRNA, reducing its interference in RNA sequencing results.Key Features:Specifically designed to deplete rRNA from prokaryotic RNA samples.Efficient Removal: Significantly reduces rRNA levels, enriching non-ribosomal RNA for accurate analysis.Streamlined Process: Simplifies sample preparation, ensuring high-quality RNA for subsequent applications.Rigorous Quality Control: Each batch undergoes thorough testing to ensure consistent performance. Applications: RNA sequencing for prokaryotes Transcriptome analysis of bacterial and other prokaryotic samplesSeamless Compatibility:Fully compatible with Genaxxon’s FAST RNA-seq Library Prep Kit, offering an integrated workflow for RNA capture and library preparation.

Genaxxon’s Unique Dual Index Midi Set is designed for precise and efficient sample preparation in Next Generation Sequencing on Illumina platforms. This set includes 24 unique dual index primer pairs (UDI Primers 1-24), ideal for small to medium-scale projects.Key Features:High Ligation Efficiency: Maximizes library output with minimal adapter dimer formation, ensuring superior performance.Enhanced Detection Accuracy: Effectively prevents data crosstalk, crucial for low-frequency mutation detection and low-abundance pathogen analysis.Truncated Adapters: Includes 15 μM truncated adapters with an 8-nucleotide sample index length for accurate and reliable indexing.Applications: Ideal for DNA or RNA library preparation on Illumina platforms, perfectly compatible with the 24 reactions of: Genaxxon DNA Library Prep Kit for Illumina (M4400) Genaxxon DNA Library Prep Kit PLUS for Illumina (M4401)

Genaxxon’s Unique Dual Index Set A is designed for precise and efficient sample preparation in Next Generation Sequencing on Illumina platforms. This set includes 48 unique dual index primer pairs (UDI Primers 1-48), ideal for medium-scale to large projects.Key Features:High Ligation Efficiency: Maximizes library output with minimal adapter dimer formation, ensuring superior performance.Enhanced Detection Accuracy: Effectively prevents data crosstalk, crucial for low-frequency mutation detection and low-abundance pathogen analysis.Truncated Adapters: Includes 15 μM truncated adapters with an 8-nucleotide sample index length for accurate and reliable indexing.Applications: Ideal for DNA or RNA library preparation on Illumina platforms, perfectly compatible with the 96 reactions of (either 2xUDI Set A or UDI Set A combined with UDI Set B): Genaxxon DNA Library Prep Kit for Illumina (M4400) Genaxxon DNA Library Prep Kit PLUS for Illumina (M4401)

Genaxxon’s Unique Dual Index Set B is designed for precise and efficient sample preparation in Next Generation Sequencing on Illumina platforms. This set includes 48 unique dual index primer pairs (UDI Primers 49-96), ideal for medium-scale to large projects.Key Features:High Ligation Efficiency: Maximizes library output with minimal adapter dimer formation, ensuring superior performance.Enhanced Detection Accuracy: Effectively prevents data crosstalk, crucial for low-frequency mutation detection and low-abundance pathogen analysis.Truncated Adapters: Includes 15 μM truncated adapters with an 8-nucleotide sample index length for accurate and reliable indexing.Applications: Ideal for DNA or RNA library preparation on Illumina platforms, perfectly compatible with the 96 reactions of (either 2xUDI Set A or UDI Set A combined with UDI Set B): Genaxxon DNA Library Prep Kit for Illumina (M4400) Genaxxon DNA Library Prep Kit PLUS for Illumina (M4401)

Genaxxon NGSQuant dsDNA HS Assay Kit for Qubit is a highly efficient, fluorescence-based solution for rapid and sensitive quantification of double-stranded DNA (dsDNA). This kit provides accurate measurements for initial DNA concentrations ranging from 10pg/µL to 100ng/µL, making it ideal for a variety of applications, including next-generation sequencing (NGS) and DNA library quantification. With its easy-to-use format and compatibility with Qubit fluorometers, the kit delivers fast, reliable, and precise dsDNA measurements for laboratories focused on high-quality data. Highlights Fast and accurate quantificationPrecise dsDNA measurement for concentrations ranging from 10pg/µL to 100ng/µLSelective detectionHigh contaminant tolerance - Performs reliably even in the presence of salts, solvents, detergents, and proteins. Complete kit – Includes concentrated assay reagent, dilution buffer, and DNA standards. Optimized for NGS – Perfect for quantifying input DNA for next-generation sequencing and DNA library quantification. Applications The NGSQuant dsDNA HS Assay Kit can be used for:Quantification of input DNA for next-generation sequencing (NGS)DNA library quantificationSuitable for use with Qubit fluorometers for precise dsDNA measurements

Genaxxon NGSQuant 1X dsDNA HS Assay Kit is a rapid, highly sensitive, and accurate solution for the quantitative detection of double-stranded DNA (dsDNA) using fluorescence. This kit is specifically designed for dsDNA, offering excellent linearity in the range of 0.2 ng to 100 ng, with a quantitation range of 10 pg/µL to 100 ng/µL.The user-friendly kit provides a ready-to-use working solution that simplifies the dsDNA quantification process on Qubit® fluorometers or fluorescence microplate readers. It is an ideal choice for large-scale DNA sample quantification in next-generation sequencing (NGS) applications, including input DNA and DNA library quantification. Additionally, the kit demonstrates robust tolerance to common contaminants, such as proteins and salts, ensuring reliable results across various sample types.Highlights Rapid, highly sensitive, and accurate fluorescent detection of double-stranded DNA (dsDNA). Excellent linearity in the range of 0.2 ng to 100 ng. Quantitation range of 10 pg/µL to 100 ng/µL. Ready-to-use working solution for easy operation. Compatible with Qubit® fluorometers and fluorescence microplate readers. Tolerant to common contaminants such as proteins and salts. Applications The NGSQuant 1X dsDNA HS Assay Kit is suitable for: Large-scale DNA sample quantification for next-generation sequencing (NGS). Input DNA quantification for NGS workflows. DNA library quantification for various sequencing applications.