LyoBalls - pre-dispensed in 96-well PCR plate or strips



Order number: M3070.0008

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While using our LyoBalls there is no need for cooling anymore! The balls and all ingredients are... more
Product information "LyoBalls - pre-dispensed in 96-well PCR plate or strips"

While using our LyoBalls there is no need for cooling anymore! The balls and all ingredients are unopened stable at ambient temperature for at least 24 months! Free sample (1 strip of 8 tubes) available here! No shipping costs within Germany.

Lyophilized (freeze-dried) temperature stable, pre-mixed and pre-dispensed Hotstart master mix with all necessary components for probe realtime PCR (optimized buffer, dNTPs, Hotstart Taq) for difficult to amplify templates, qPCR and Multiplex applications.

No cooling/cooling chain necessary! The beads and all ingredients are unopened stable at ambient temperature for at least 24 months!

Our Hotstart Polymerase guarantees that during setup and the first PCR cycle the enzyme is not active and misprimed primers are not extended. As a result specificity and efficiency are increased by far compared to standard Taq DNA polymerase. Additionally, difficult targets with high GC-content can be amplified. The higher sensitivity improves multiplex PCR results.
This lyophilized master mix in a easy to handle bead format is the perfect choice for a fast reaction setup that reduces the time required for pipetting and the possibility for pipetting errors. Just add  PCR-grade water, template and primers to reconstitute the master mix and you are all set!

Lyophilized realtime PCR master mix:

•    Hotstart Polymerase
•    increased specificity
•    increased sensitivity
•    for difficult to amplify templates with high GC content
•    for Multiplex applications
•    for qPCR
•    ready-to-use
This lyophilized Hotstart master mix is delivered in single beads that can be easily dispensed. Each bead is equivalent to one 20µL PCR reaction.

Our lyophilized qPCR Probe master mix is available in powder form (LyoMix, M3071) and pre-dispensed in a 96-well PCR plate or strips.

We also offer our SuperHot Hotstart Master Mix with and without dye.

With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.

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Our comment on "LyoBalls - pre-dispensed in 96-well PCR plate or strips"
- pre-dispensed, freeze-dried realtime PCR reagents mix in bead format. - only add template DNA solution and primers, and cycle. - less risk of pipetting errors and contamination thus more reliable and reproducible. - long-term ambient-temperature stable
Specifications: LyoBalls are a ready-to-use freeze-dried master mix that contains all... more
 

Technical Data:

Specifications:
LyoBalls are a ready-to-use freeze-dried master mix that contains all necessary components for realtime PCR.
Aliquot size: 1 bead = 1 PCR reaction.
standard pack format: predispensed in a Break-a-way PCR plate (0.2mL wells). Plate easily breakable in strips of 8 wells. Wells closed with 8 cap strips or as 8 tube strips with attached single lids (0.2mL tubes).

This master mix is stable for at least 24 months at ambient temperature if the supplied tube is kept unopened!

application:

For multiplex PCR. For Real-time PCR. For PCR of low amount of DNA (low copy number).

Unit Definition:

One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.

Source

recombinant

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

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