Order number: M3013.0100Shipping: shipped on wet ice and stored at -20°C
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
Multiplex PCR is a method that enables amplification of two or more amplicons simultaneously in a single reaction tube/reaction. It is widely used in genotyping and different areas of DNA testing in research, forensic and diagnostic laboratories.
Our Multiplex HS Mastermix (2X) is a ready-to-use PCR mixture is an optimized ready-to-use mixture for probe-based assays such as TaqMan®, Beacons and MGBs. It contains a modified fast HotStart Taq DNA Polymerase, dNTPs and MgCl₂ combined in an optimized buffer system for realtime PCR / qPCR applications except primers, probe and template DNA / cDNA.
The HotStart Taq Polymerase is based on the standard Taq DNA polymerase from Genaxxon inactivated by an specific antibody against Taq DNA polymerase which is activated by heat treatment. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity, increased sensitivity and greater yields when compared to standard DNA polymerases, making this enzyme especially well-suited for multiplex PCR.
- Amplification of multiple targets in a single tube
- All-in-one master mix for convenient multiplexing
- High specificity, sensitivity and product yield
- Easy reaction setup at room temperature
The Multiplex HS Master mix 2X is shipped in aliquots of 1mL.
Other realtime master mixes for your realtime PCR experiments can be found here >.
Examples of Multiplex applications:
F. Javier Pérez-Pérez and Nancy D. Hanson, Detection of Plasmid-Mediated AmpC β-Lactamase Genes in Clinical Isolates by Using Multiplex PCR, J. Clin. Microbiol. June 2002 vol. 40 no. 6 2153-2162. doi: 10.1128/JCM.40.6.2153-2162.2002.
Tamara B. Souzaa, Diego M. Lozerb, Sônia M. S. Kitagawab, Liliana C. Spanob, Neusa P. Silvac and Isabel C. A. Scaletskya, Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli. J. Clin. Microbiol. March 2013 vol. 51 no. 3 1031-1033, doi: 10.1128/JCM.02478-12.
The Multiplex master mix for fast and easy multiplexing minimizes the need for optimization and makes the development of multiplex PCR assays fast and easy. - Amplification of multiple targets in a single tube - All-in-one master mix for convenient multiplexing - High specificity, sensitivity and product yield - Easy reaction setup at room temperature
2-time ready-to-use multiplex master mix with an optimized modified Taq DNA polymerase without green fluorescent dye and without ROX™.
application:Automated hotstart PCR Gene expression Multiplex qPCR / Multiplex PCR with fluorochromes Genotyping Copy number analysis
Unit Definition:One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads
Here you will find information and further literature on Multiplex HS MasterMix (2X). For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.