Bio Lp-1 qPCR Kit for purification and detection of Legionella spp., L. pneumophila serogroups
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The Bio Lp-1 assay is designed to target specific and unique DNA sequences within the Legionella genome. The PCR products from 4 different reactions can be detected by specific DNA probes labeled with 4 different fluorophores. This allows the simultaneous detection of all the Legionella species associated with infection, all the L. pneumophila serogroups (1-16) and specifically identifies L. pneumophila sg-1, responsible for ~95% human infections. An internal amplification control is also included in the master mix as an indicator that the PCR is functioning correctly.
Designed to work on the leading real-time PCR platforms, Bio Lp-1 rapidly detects extremely low levels of Legionella, and can differentiate between Legionella spp, all L. pneumophila serogroups 1-16 and L. pneumophila-serogroup 1simultaneously in a single water sample, giving a complete and accurate Legionella profile. With this certainty, the exact risk to public health can be quickly determined and acted upon. Particularly beneficial in cases of an outbreak, appropriate remedial actions can be taken, their efficacy monitored to reduce and remove Legionella bacteria from a contaminated water system. In less than 5 hours, water quality and safety can be assured. Simple and convenient to use, and in a freeze-dried format, the test contains all necessary components to process a water sample directly.
Bio Lp-1-Legionella multiplex one step qPCR Kit is a diagnostic breakthrough, being the world’s first open platform rapid NAD kit for simultaneously detecting and identifying multiple species of Legionella from water in a single test.
The lyophilized master mix is aliquoted into 12 x 8 low profile PCR Tube strips packed in an aluminium foil bag together with a DNA purification kit and with negative and positive DNA control enabling to prepare standards of 10E5 GU, 10E4 GU, 10E3 GU, 10E2 GU, 10E1 GU (with 1 GU = one genomic unit) and a DNA purification kit that is optimized for the isolation of Legionella DNA isolated from water samples.
| Description |
Bio Lp-1 Legionella Quantification Kit |
| Sample type | Water |
| Cycler compatibility | Roche Diagnostics LightCycler 480 system II, Bio-Rad CFX96 Touch, Thermo QuantStudio |
| Basis for detection | Probe based |
| Format | Lyophilized powder (PCR assay, Positive control DNA, PCR standards), Liquid (Negative control) |
| No. of reactions |
96 |
| Components | qPCR assay - 12 x 8 well qPCR strips (foil wrapped) |
| Positive control DNA - lyophilised | |
| Negative control - liquid | |
| Legionella DNA for quantification - lyophilised | |
| Bio Lp-1 DNA Isolation Kit | |
| PCR method | qPCR multiplex |
| Storage | Room temperature. Positive control and DNA for quantification, +2°C to +8°C. -20°C after rehydration. |
| PCR efficiency |
88.12 - 99.46% |
|
Quantification limit |
15 GU |
|
Detection limit |
5 GU |
| Use | Water testing |
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-10-90
Dokumente:
ProtokolleDatenblätter
Quelle: NCBI PubMed
Legionella References
- Legionella (Legionnaires' Disease and Pontiac Fever) Treatment and Complications. Centers for Disease Control and Prevention (CDC). 26 January 2016. Archived from the original on 29 March 2016. Retrieved 21 March 2016.
- Verhasselt et al. (2021) COVID-19 Co-infection with Legionella pneumophila in 2 Tertiary-Care Hospitals, Germany. Emerging Infectious Diseases, Vol27, No5
- Ashbolt, N. J. (2021) Legionella Infection during and after the COVID-19 Pandemic, ACS EST Water, Vol1, pp13-14
- Palazzolo, C. et al. (2020) Legionella pneumonia: increased risk after COVID-19 lockdown? Italy, May to June 2020, Eurosurveillance, Vol25, No30
- Legionella Control, https://legionellacontrol.com/international/us-covid-shutdown-legionnaires-outbreaks/ (accessed on 20 April 2021)
- ...
List of bacteria that have been tested to be not detected with our Legionella Multiplex PCR kit. These bacteria will be part of the ISO 12869 certification.
Aeromonas hydrophila
Alcaligenes faecalis (e.g. CIP 60.80)
Bacillus subtilis (e.g. CCM 1999)
Burkholderia cepacia
Clostridium spp.
Enterobacter aerogenes
Escherichia coli (e.g. WDCM 00003)
Flavobacterium spp.
Klebsiella oxytoca (e.g. WDCM 00179)
Listeria monocytogenes (e.g. WDCM 00109 or WDCM 00020)
Proteus vulgaris
Pseudomonas aeruginosa
Pseudomonas fluorescens
Pseudomonas putida
Serratia marcescens
Stenotrophomonas maltophila.
These bacteria have been tested as part of the ongoing certification process and all have come up negative when using the Bio Lp-1 kit for detection of Legionella.
