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Multiplex qPCR Kit for detection Legionella ssp., L. pneumophila sg (1-16) , L. pneumophila sg 1

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  • Hochwertige Produkte
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1.225,00 €*

Produktnummer: L3001.0096
Sofort versandfertig, Lieferzeit 1-2 Werktage

Shipment: not cooled. Store parts at +2°C to +8°C. For laboratory usage only!
Produktinformationen "Multiplex qPCR Kit for detection Legionella ssp., L. pneumophila sg (1-16) , L. pneumophila sg 1"

The Legionella Detection Multiplex qPCR Assay is designed to target specific and unique DNA sequences within the Legionella genome. The PCR products from 4 different reactions can be detected by specific DNA probes labeled with 4 different fluorophores. This allows the simultaneous detection of all the Legionella species associated with infection, all the L. pneumophila serogroups (1-16) and specifically identifies L. pneumophila sg-1, responsible for ~95% human infections. An internal amplification control is also included in the master mix as an indicator that the PCR is functioning correctly.

Designed to work on the leading real-time PCR platforms, Bio Lp-1 rapidly detects extremely low levels of Legionella, and can differentiate between Legionella spp, all L. pneumophila serogroups 1-16 and L. pneumophila-serogroup 1 simultaneously in a single water sample, giving a complete and accurate Legionella profile. With this certainty, the exact risk to public health can be quickly determined and acted upon. Particularly beneficial in cases of an outbreak, appropriate remedial actions can be taken, their efficacy monitored to reduce and remove Legionella bacteria from a contaminated water system. In less than 5 hours, water quality and safety can be assured. Simple and convenient to use, and in a freeze-dried format, the test contains all necessary components to process a water sample directly.

Bio Lp-1-Legionella multiplex one step qPCR Kit is a diagnostic breakthrough, being the world’s first open platform rapid NAD kit for simultaneously detecting and identifying multiple species of Legionella from water in a single test.

The lyophilized master mix is aliquoted into 12 x 8 low profile qPCR strips (foil wrapped).

This kit comes with negative and positive DNA control enabling to prepare standards of 10E5 GU, 10E4 GU, 10E3 GU, 10E2 GU, 10E1 GU (with 1 GU = one genomic unit).

Description

Lyophilized Multiplex qPCR Assay

Sample type Water
Cycler compatibility Roche Diagnostics LightCycler 480 system II, Bio-Rad CFX96 Touch
Basis for detection Probe based
Format Lyophilized powder (PCR assay)
No. of reactions

96

Components qPCR assay -12 x 8 low profile qPCR strips (foil wrapped)
  Positive control DNA - lyophilised
  Negative control DNA - liquid
PCR method qPCR multiplex
Storage Ambient temperature.
PCR efficiency

88.12 - 99.46% 

Quantification limit

15 GU

Detection limit

5 GU

 Use Water testing

Applikation / Application:
drinking water test on legionella, waste water test on legionella, process water test on legionella

Einheiten / Units:
Quelle / Source:


Sicherheits Hinweise / Safety


Klassifizierungen / Classification

eclass-Nr: 32-16-10-90
Dokumente - Protokolle - Downloads :
Hier finden Sie Informationen und weiterführende Literatur. Für weitere Dokumente (Zertifikate mit weiteren Lotnummern, Sicherheitsdatenblätter in anderer Sprache, weitere Produktinformationen) wenden Sie sich bitte an Genaxxon biosience unter: info@genaxxon.com oder Tel.: +49 731 3608 123.


Dokumente:

Protokolle
Datenblätter
Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Quelle: NCBI PubMed

Legionella References

  • Legionella (Legionnaires' Disease and Pontiac Fever) Treatment and Complications. Centers for Disease Control and Prevention (CDC). 26 January 2016. Archived from the original on 29 March 2016. Retrieved 21 March 2016.
  • Verhasselt et al. (2021) COVID-19 Co-infection with Legionella pneumophila in 2 Tertiary-Care Hospitals, Germany. Emerging Infectious Diseases, Vol27, No5
  • Ashbolt, N. J. (2021) Legionella Infection during and after the COVID-19 Pandemic, ACS EST Water, Vol1, pp13-14
  • Palazzolo, C. et al. (2020) Legionella pneumonia: increased risk after COVID-19 lockdown? Italy, May to June 2020, Eurosurveillance, Vol25, No30
  • Legionella Control, https://legionellacontrol.com/international/us-covid-shutdown-legionnaires-outbreaks/ (accessed on 20 April 2021)
  • ...

List of bacteria that have been tested to be not detected with our Legionella Multiplex PCR kit. These bacteria will be part of the ISO 12869 certification.

Aeromonas hydrophila
Alcaligenes faecalis (e.g. CIP 60.80)
Bacillus subtilis (e.g. CCM 1999)
Burkholderia cepacia
Clostridium spp.
Enterobacter aerogenes
Escherichia coli (e.g. WDCM 00003)
Flavobacterium spp.
Klebsiella oxytoca (e.g. WDCM 00179)
Listeria monocytogenes (e.g. WDCM 00109 or WDCM 00020)
Proteus vulgaris
Pseudomonas aeruginosa
Pseudomonas fluorescens
Pseudomonas putida
Serratia marcescens
Stenotrophomonas maltophila.

These bacteria have been tested as part of the ongoing certification process and all have come up negative when using the Bio Lp-1 kit for detection of Legionella.