Vorteile im Überblick
- Hochwertige Produkte
- Kundenspezifische Lösungen
- Persönlicher Kontakt
- Schneller Service
- Kompetenter technischer Support +49(0)731-3608-123
- Kostenloser Versand
- Versand innerhalb von 24h
- 30 Tage Geld-Zurück-Garantie
- Bequemer Kauf auf Rechnung
Produktinformationen "RealQ Plus 2x Master Mix Green - High ROX"
Introduction:
Quantitative PCR is an important tool for SNP and gene expression analysis. Two general fluorescent chemistries exist for quantitative detection of gene transcripts: probes (e.g. TaqMan®, Scorpions™ Probes, molecular beacons) and DNA-binding fluorescent dyes (e.g. ethidium bromide, SYBR® Green, EvaGreen®, PicoGreen®). Ampliqon offers the RealQ Plus 2x Master Mix in two formulations: for probe or with DNA-binding fluorescent dye, making them ideal for most quantitative PCR applications.
The RealQ Plus 2x Master Mixes are available with high, low or without ROX™ for optimal performance on most of the commonly used real-time PCR instruments. The RealQ Plus 2x Master Mixes promote high specificity and low background by using TEMPase Hot Start DNA Polymerase, a modified Taq DNA polymerase with hot start capabilities.
The RealQ Plus 2x Master Mix Green with high ROX™ is a singletube 2x reagent including all components necessary to perform DNA-binding dye based real-time DNA amplification. Just add primers and DNA. ROX™ internal reference dye level is optimized for the popular StepOne and StepOnePlus instruments from Life Technologies.
Detection limit: approximately 1 copy.
Quantitation limit: approximately 24 copies (0.08ng of human gDNA, correlating to 12 diploid genomes, with 2 gene copies per diploid genome)
Compatibility: Real-time instruments that require high ROX™ as internal reference dye e.g. StepOne and StepOnePlus from Life Technologies.
Specifications:
The GreenMastermix is a ready-to-use 2-time mastermix that contains a green fluorescence dye, dUTP instead of dTTP and 500nM ROX™ (high ROX concentration) as reference dye.
Applikation:
Low-copy targets PCR Real-time PCR Quantitative PCRKlassifizierungen:
Dokumente:
Spez. ProduktbeschreibungAllg. Daten 1
Allg. Daten 2
Allg. Daten 3
Allg. Daten 4
Kategorienliste