Real-time PCR, also known as qPCR, is undoubtedly an impressive method for measuring the amplification of target DNA in real time. However, some people may despair at the abundance of terms, protocols and kits on the market. This begs the question: are reliable qPCR results only achievable through magic or is it ultimately more about the art of precise fine-tuning? We can reassure you: in fact, with just a few tricks you can optimize your qPCR so that you can easily achieve reliable and accurate results. Whether you are already an expert in the field of qPCR or just taking your first steps into this fascinating world, this article will guide you through the key factors that will ensure your qPCR assays produce the best possible results. From deciding on the method, to quality control, to optimizing specificity - we have important tips and suggestions for you.

Since the isolation of the first Taq DNA polymerase in 1976, PCR has become an integral part of the molecular biology laboratory. Of course, methods, reagents and protocols have evolved. The goal is clear: to make it better, faster, cheaper, and easier. Every day, lab technicians, students and scientists around the world spend countless hours pipetting and preparing PCR experiments. This makes it even more frustrating when errors and contamination only become apparent in the final step of the PCR workflow. But how can the work in the PCR laboratory be optimised to ultimately minimise sources of error and at the same time save time, money, and nerves? We at Genaxxon would like to shed some light into the darkness of the laboratory and show how our new products around the Red Mastermix Fluoro (2X) can help to optimise your PCR workflow along a typical PCR workflow.

Extraction and isolation of viral RNA is the first step in the detection and testing of viral RNA by real-time RT-PCR. Here we compare different methods and protocols for extraction of viral RNA. Nested RT-PCR and real-time RT-PCR are the most common methods for the analysis of viral infections (see Robert Koch Institute >). In contrast to examinations with ELISA or IFA, this can be done at an earlier stage of infection.

Non-protein-coding RNAs (ncRNAs) are expressed in viruses, archaea, prokaryotes and eukaryotes. They fulfil vital roles in the regulation of chromatin architecture, epigenetic memory, transcription, RNA splicing, editing and turnover. To date, at least 18 distinct types of ncRNA have been identified, which are classified according to their size, function, subcellular localization and target specificity.

In the articles below you will find the authors already relying on the Genaxxon products. We say thank you to these authors, trusting in our service. Let us convince you as well.

Nowadays there are many different suppliers and systems for polymerase chain reaction (PCR) and of its derivative techniques, quantitative, or real-time, PCR (qPCR) and reverse transcription PCR (RT-PCR). Competition gives you the opportunity to simplify diagnostics and to make research more financially favorable.

There's no accounting for taste. Cilantro (Coriander): some people love it in their food, some hate it. Here we are detecting a genomic SNP (rs72921001) in HeLa genomic DNA. This SNP is reported to be close to a number of genes coding for olfactory receptors. (Reference: Eriksson N. et al. (2012), "A genetic variant near olfactory receptor genes influences cilantro preference.") Considering, that only the C-allele specific primer is extended and yielding in a specific amplicon, we can conclude a genetic predisposition in disliking cilantro, as this SNP is significantly associated with detecting a soapy taste to cilantro.

Listed below are articles and references, in which the authors trust in the high quality of this GENAXXON Product. Source: PubMed NCBI > Immunomodulatory role of reactive oxygen species and nitroge...