ReproFast proofreading Polymerase



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ReproFast high-fidelity DNA polymerase provides high overall performance and more robust... more
Product information "ReproFast proofreading Polymerase"

ReproFast high-fidelity DNA polymerase provides high overall performance and more robust amplification of longer targets due to Genaxxons polymerase-enhancing factor. The use of ReproFast polymerase enhances overall PCR performance, including shorter extension times, higher yield and greater target length capability compared to that from standard Pfu- or Pwo DNA polymerase.

Test sample available at a special price! No shipment costs within Germany. The test sample price will be refunded on the first official order of the product.

The main application of our ReproFast is for amplification of long target sequences, including genomic DNA giving high accuracy for cloning experiments. With ReproFast it will be possible to amplify fragments up to 5 kb from human genomic DNA and up to 7 kb from lambda DNA (Barnes (1994) Proc. Natl. Acad. Sci. USA 91. 2216-2220) with the accuracy of Pfu- or Pwo polymerases. The Genaxxon bioscience ReproFast DNA-Polymerase is a thermostable enzyme possessing 5‘-3‘ DNA polymerase and 3‘-5‘ proofreading exonuclease activities (generates no A-overhangs / suitable for blund-end cloning).

With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.

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Our comment on "ReproFast proofreading Polymerase"
3' - 5' Exonuclease activity (proof-reading activity)
5 U/µL 3' - 5' Exonuclease activity (proof-reading activity) more
 

Technical Data:

5 U/µL

3' - 5' Exonuclease activity (proof-reading activity)

application:

Polymerase mixture to be used for long fragments in PCR

Unit Definition:

One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C), 50 mM

Source

recombinant

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

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