Cytokines and Growth Factors

Kinetin is an analogue to Zeatin, an adenine-type cytokinin phytohormone that is used in plant culture media such as Murashige & Skoog media in conjunction with auxins such as indole-3-acetic acid (IAA), Indole-3-butyric acid (IBA), 1-napthaleneacetic acid (NAA) or others to induce the fomation of callus and to regenerate plant tissues from callus. Zeatin the active ingredient in coconut milk is not very stable in cell culture and therefor often replaced by the structurell related Kinetin (C4304) >. Both substances promote plant growth. Other active phytohormones are: Thidiazuron (TDZ) > and 6-Benzylaminopurine (6-BAP). Antibiotics used in plant cell culture are: Rifampicin, Carbenicillin or Kanamycin sulfate (M3129) >. Synonyms: 6-Furfurylaminopurine; N6-Furfuryladenine; Furfuryl(purin-6-yl)amine

FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. there are differences in the tissue distribution and concentration of these 2 growth factors. Rec. bovine FGF-2 produced in E.Coli is a single, non-glycosylated, polypeptide chain of 155 amino acids and a molecular mass of 17250 Dalton. Synonyms: HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b.

Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. Rec. human Fibroblast Growth Factor-2 (FGF-2 / FGF-basic) produced in E.Coli is a single, non-glycosylated, polypeptide chain of 154 amino acids and a molecular mass of 17.2kDa. Biological activity: The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5ng/mL, corresponding to a specific activity of 2,000,000 Units/mg. Amino acid sequence: AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS.

GCSF is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Three transcript variants encoding three different isoforms have been found for the GCSF gene. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This csf induces granulocytes. Rec. human Granulocyte Colony Stimulating Factor produced in E.coli is a single, non-glycosylated, polypeptide chain of 175 amino acids and a molecular mass of 18.8 KDa. Biological activity: The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is <0.1ng/mL, corresponding to a Specific Activity of 100,000,000 IU/mg. Synonyms: CSF-3, MGI-1G, GM-CSF beta, Pluripoietin, Filgrastim, Lenograstim, G-CSF, MGC45931, GCSF.

Recombinant Human IL-6 produced in E.Coli is a single, non-glycosylated polypeptide chain of 184 amino acids and a molecular mass of 21,000 Dalton. IL-6 is a cytokine with a wide variety of biological functions: it plays an essential role in the final differentiation of b-cells into ig-secreting cells, it induces myeloma and plasmacytoma growth, it induces nerve cells differentiation, in hepatocytes it induces acute phase reactants. Synonyms: IFN-b2, B cell differentiation factor, BCDF, BSF-2, HPGF, HSF, MGI-2, B-cell stimulatory factor 2, Interferon beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, IL-6, HGF. Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of murine TF-1 cells was found to be <0.1ng/mL, corresponding to a Specific Activity of 1x10E7 IU/mg.

Recombinant Human IL-7 produced in E.Coli is a single, non-glycosylated polypeptide of 172 amino acids and a molecular mass of 17,412 Dalton. The ED50 as determined by the dose-dependant stimulation of murine IXN/2B cells is <0.3 ng/mL, corresponding to a Specific Activity of 1 x 10(6) IU/mg. IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.

Packungsgröße: 2 µg Quelle: Escherichia Coli. Technische Daten: Purity: > 98.0% (by RP-HPLC, IEX-HPLC, SDS-PAGE). Less than 1% dimers and aggregates. Sterile Filtered White lyophilized (freeze-dried) powder. The lyophilized rHuIL-7 is soluble in water and most aqueous buffers below and above the isoelectric point. Endotoxin: Less than 0.1 ng/µg (IEU/µg) rHuIL-9. Lagerung / Transport: shipped at RT, stored at -20°C Produkt-Nummer: C6076.0002 changedate: 30.12.2013 Produktdetails: rec. humanes Interleukin-9 (rHuIL-9) Produkt Details (pdf-Dokument)

Recombinant Human PDGF-BB produced in E.Coli is a double, non-glycosylated, polypeptide chain containing 109 amino acids and having a molecular mass of 24,525 Dalton. The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is <0.1ng/mL, corresponding to a Specific Activity of 1 MIU/mg. PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene.

Recombinant Human SCF produced in E.Coli is a single, non-glycosylated polypeptide chain of 164 amino acids and a molecular mass of 18,409 Dalton. Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid). The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Gly-Ile-Cys. Synonyms: Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250. Biological Activity:The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is <2ng/mL, corresponding to a Specific Activity of 5 x 10E5 IU/mg.

Description:Vascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g. stimulation monocyte/macrophage migration, neurons, cancer cells, kidney epithelial cells ). VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. The recombinant human VEGF is isolated from E.Coli  as a double, non-glycosylated, polypeptide of 165 amino acids (each dimer) and a total molecular mass of approx. 39kDa. Amino acid sequence: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR. Biological Activity:The biological activity was determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 3.7-5.6 ng/mL, corresponding to a Specific Activity of 178,570 to 270,270 IU/mg.

Granulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. MCSF induces cells of the monocyte/macrophage lineage. MCSF plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Rec. human Macrophage Colony Stimulating Factor produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain of 2 x 159 amino acids and a total molecular mass of 36.8 KD. Synonyms: Macrophage Colony Stimulating Factor, CSF-1, Lanimostim, MCSF, MGC31930, M-CSF.

Recombinant human Thrombopoietin (rHuTPO) expressed and isoltated from HEK cells is a single, non-glycosylated polypeptide of 332 amino acids and a molecular mass of 78-84 kDa depending on the glycosylation. Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidney hat regulates the production of platelets by the bone marrow. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that fragment into large numbers of platelets. Part of the protein (N-terminal domain) is very similar to erythropoietin, the hormone necessary for the production of erythrocytes (red blood cells). Biological Activity:The activity was determined by the dose-dependent stimulation of the proliferation of MO7e cells, the ED50 is 1.15ng/mL. Amino acid sequence: SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNELPNRTSG LETNFTASA RTTGSGLLKW QQGFRAKIPG LLNQTSRSLD QIPGYLNRIH ELLNGTRGLF PGPSRRTLGA PDISSGTSDT GSLPPNLQPG YSPSPTHPPT GQYTLFPLPP TLPTPVVQLH PLLPDPSAPT PTPTSPLLNT SYTHSQNLSQ EG Synonyms: Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO.

Thidiazuron (TDZ) a urea derivative which acts as a cytokinin-active plant growth regulator used in micropropagations to supplement media such as Murashige and Skoog medium. TDZ promotes plant organogenesis (shoot regeneration) and plant regeneration. Thidiazuron (TDZ) enables the production of virus-free stock plants, speeds up breeding, and provides new genotypes for the market. Synonyms: 6-Furfurylaminopurine; N6-Furfuryladenine; Furfuryl(purin-6-yl)amine. Other active phytohormones are: Kinetin (C4304) >, Zeatin (C4301) > and 6-Benzylaminopurine (6-BAP). Antibiotics used in plant cell culture are: Rifampicin, Carbenicillin or Kanamycin sulfate (M3129) >.

Zeatin, a phytohormone derived from adenine, belongs to the family of plant growthhormones called cytokinins. Zeatin promotes growth of lateral buds, stimulates cell division, callus initiation and seed germination. Zeatin is used with Murashige & Skoog and other media to promote multiple shoot generation from internodes (promotion of growth of lateral buds giving bushier plants) and high frequency thick spread roots. Zeatin the active ingredient in coconut milk is not very stable in cell culture and therefor often replaced by the structurell related Kinetin (C4304) >. Both substances promote plant growth. Other active phytohormones are: Thidiazuron (TDZ) > and 6-Benzylaminopurine (6-BAP). Antibiotics used in plant cell culture are: Rifampicin, Carbenicillin or Kanamycin sulfate (M3129) >. Zeatin has several anti-aging effects on human skin fibroblasts.