DNA Polymerases for PCR - DNA Polymerases for PCR

High-quality Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience Taq Polymerase allows amplification of DNA fragment of up to >7 kb. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer and separate MgCL2.The enzyme is delivered with our buffer component 'Buffer-S'. The buffer is optimised for high specificity amplification of DNA-templates. Our complete buffer contains 15mM MgCl2. Free Taq DNA Polymerase test sample available!No shipping costs within Germany. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.

High-quality Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience Taq Polymerase allows amplification of DNA fragment of >7 kb. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer and separate MgCL2.The enzyme is delivered with our buffer component 'Buffer-E'. The buffer is optimised for high yield amplification of DNA-templates. Our complete buffer contains 25mM MgCl2. Taq DNA Polymerase test sample available! No shipping costs within Germany. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.

Genaxxon's FAST DNA polymerase is a robust enzyme, ideally suited for applications like genotyping and screening, amplifying with extreme speed of up to 2 seconds per 1kb, as well as high yield and consistency. FAST DNA polymerase has 5’-3’ exonuclease activity, but no 3’-5’ exonuclease (proofreading) activity. PCR products generated with this enzyme are A-tailed and can thus be cloned into TA cloning vectors. The extreme speed of Genaxxons' FAST DNA polymerase allows the use of an extension rate of 4-8 kb/min. Genaxxon's FAST DNA Polymerase is delivered with 5X reaction buffer already containing MgCL2, dNTPs, enhancers and stabilizers for an optimal PCR result. Genaxxon's FAST DNA polymerase is also available as a convenient 2x master mix (M3286), including an inert red tracking dye for electrophoresis, containing all required components for fast PCR, except specific primers. 

Genaxxon’s FAST HotStart DNA polymerase is a robust enzyme with a hotstart function, ideally suited for applications like genotyping and screening, amplifying with extreme speed of up to 2 seconds per 1kb, as well as high yield and consistency. PCR products generated with this enzyme are A-tailed and can thus be cloned into TA cloning vectors. The extreme speed of Genaxxon’s FAST HotStart DNA polymerase allows the use of an extension rate of 4-8 kb/min. The buffer composition has been optimized via high-throughput screening and makes the enzyme particularly resistant to PCR inhibitors. Therefore, Genaxxon’s FAST HotStart DNA polymerase is suitable for direct PCR from unpurified samples, including blood, urine, and bacterial colonies, making this the ideal choice for consistent results in fast complex PCR amplifications. Genaxxon's FAST HotStart DNA polymerase is also available as a convenient 2x master mix (M3288 >), including an inert red tracking dye for electrophoresis, containing all required components for fast PCR, except specific primers. 

Taq DNA Hot Start Polymerase with Aptamer technology. The specific aptamer inhibits DNA polymerase activity below temperatures of 50°C to 55°C, thus enabling hot start PCR. At higher temperatures, the aptamer falls off the active site of Taq polymerase. The Hot Start Taq Polymerase can be used like a normal Taq without having to lengthen the first denaturation step.The binding of aptamer and polymerase prevents the formation of primer-dimer constructs and other artefacts revealed by non-specific primer binding. Check also our SuperHot Mastermix > with or without dye. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.

SuperHot Taq DNA Polymerase is a superior DNA Polymerase for Real Time PCR, Hot-Start PCR, low-copy number PCR, or PCR of difficult templates. The enzyme is developed to enhance the specificity, sensitivity and yield of DNA amplification. SuperHot Taq DNA polymerase is a chemicaly modified form of thermostable DNA polymerase Taq, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and before the first PCR cycle, the enzyme is not active and misprimed primers are not extended. As a result specifity and efficiency are increased by far compared to standard Taq DNA polymerase. Additionally, difficult targets with high GC-content can be amplified. For realtime PCR Genaxxon offers specially optimized 2-times qPCR mastermix >, respective a special Multiplex-PCR mastermix >. Features of Genaxxons SuperHot DNA Polymerase chemically modified increased specifity increased sensitivity for difficult templates with high GC-content Please have also a look on our SuperHot Taq 2-times Mastermix >. Even more convenient than the SuperHot Taq. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and favourable standard agarose (M3044) > we can offer additional products for your PCR.

SuperHot Taq DNA polymerase is a chemicaly modified form of thermostable DNA polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first PCR cycle, the enzyme is not active and misprimed primers are not extended. As a result specifity and efficiency are increased by far compared to standard Taq DNA polymerase. Additionally, difficult targets with high GC-content can be amplified. Sensitivity improves multiplex PCR, an applied PCR technique that amplifies several specific targets simultaneously. The Genaxxon SuperHot Taq-Kit includes Genaxxon's SuperHot Taq Polymerase, buffer E, MgCl2, appropriate amounts of a 10mM dNTP-Mix sufficient for up to 500 / 2000 x 25µL PCR reactions (0.5 units Taq per reaction). All items are in shipped in separate tubes. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and favourable standard agarose (M3044) > we can offer additional products for your PCR.

The High-Fidelity Pfu proofreading DNA polymerase from Genaxxon bioscience is a thermostable, highly processive enzyme possessing 5'-3' DNA polymerase with additional 3'-5' proofreading exonuclease activity, which enables the correction of nucleotide incorporation errors. It has no 5'→3' exonuclease activity. The High-Fidelity Pfu DNA polymerase is a recombinant form of the hyperthermophilic archaebacteria Pyrococcus furiosus (Pfu). Pfun proofreading DNA polymerase shows an increased thermostability and a 10-times higher accuracy compared to Taq DNA polymerase >. A mixture of Taq DNA Polymerase and Pfu DNA polymerase provides more robust synthesis of longer amplification products (Barnes, 1994. Proc. Natl. Acad. Sci. USA 91:2216-2220). Features: 10-times higher accuracy compared to Taq DNA polymerase High-Fidelity polymerase Proofreading function (3' - 5' exonuclease activity) High thermostability Generates blunt-end PCR products Generates PCR products for cloning and expression More High-Fidelity Proofreading Polymerases from Genaxxon bioscience: - M3002 Pwo Proofreading Polymerase >- M3003 ReproFast Proofreading Polymerase >- M3004 Pfu Proofreading Polymerase >- M3012 ReproHot (KOD) Proofreading Polymerase >- M3030 ExactRun (Phusion like) Proofreading Polymerase > With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.

The High-Fidelity Pwo proofreading DNA polymerase from Genaxxon bioscience is a thermostable, highly processive enzyme possessing 5'-3' DNA polymerase with additional 3'-5' proofreading exonuclease activity, which enables the correction of nucleotide incorporation errors. It has no 5'→3' exonuclease activity. The High-Fidelity Pwo DNA polymerase is a recombinant form of the hyperthermophilic archaebacteria Pyrococcus woesei. Pwo proofreading DNA polymerase shows an increased thermostability and a 10-times higher accuracy compared to Taq DNA polymerase >. A mixture of Taq DNA Polymerase and Pwo DNA polymerase provides more robust synthesis of longer amplification products (Barnes, 1994. Proc. Natl. Acad. Sci. USA 91:2216-2220). Test sample available at a special price! The test sample price will be refunded on the first official order of the product. Features: 10-times higher accuracy compared to Taq DNA polymerase High-Fidelity polymerase Proofreading function (3' - 5' exonuclease activity) High thermo stability Generates blunt-end PCR products Generates PCR products for cloning and expression More High-Fidelity Proofreading Polymerases from Genaxxon bioscience: - M3002 Pwo Proofreading Polymerase >- M3003 ReproFast Proofreading Polymerase >- M3004 Pfu Proofreading Polymerase >- M3012 ReproHot (KOD) Proofreading Polymerase >- M3030 ExactRun (Phusion like) Proofreading Polymerase > With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.

ReproFast high-fidelity DNA polymerase provides high overall performance and more robust amplification of longer targets due to Genaxxons polymerase-enhancing factor. The use of ReproFast polymerase enhances overall PCR performance, including shorter extension times, higher yield and greater target length capability compared to that from standard Pfu- or Pwo DNA polymerase. The main application of our ReproFast is for amplification of long target sequences, including genomic DNA giving high accuracy for cloning experiments. With ReproFast it will be possible to amplify fragments up to 5 kb from human genomic DNA and up to 7 kb from lambda DNA (Barnes (1994) Proc. Natl. Acad. Sci. USA 91. 2216-2220) with the accuracy of Pfu- or Pwo polymerases. The Genaxxon bioscience ReproFast DNA-Polymerase is a thermostable enzyme possessing 5‘-3‘ DNA polymerase and 3‘-5‘ proofreading exonuclease activities (generates no A-overhangs / suitable for blund-end cloning). Test sample available at a special price! The test sample price will be refunded on the first official order of the product. More High-Fidelity Proofreading Polymerases from Genaxxon bioscience: - M3002 Pwo Proofreading Polymerase >- M3003 ReproFast Proofreading Polymerase >- M3004 Pfu Proofreading Polymerase >- M3012 ReproHot (KOD) Proofreading Polymerase >- M3030 ExactRun (Phusion like) Proofreading Polymerase > With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.

ReproHot is a high fidelity DNA polymerase for accurate PCR amplification of GC-rich templates used for cloning and cDNA amplification. This enzyme is a mixture of Taq DNA polymerase plus a proofreading polymerase and a monoclonal antibody, which blocks polymerase activity prior to heating. The complex formed between the polymerase and the antibody prevents primer-dimer and other artifacts resulting from unspecific primer binding. The antibody is inactivated while heating up above 75°C, requiring no prolonged first heating step prior to cycling. The combined hotstart and proofreading feature leads to highly specific and correct amplicons. ReproHot can be used as a cheap alternative to Phusion DNA polymerase from. NEB or other sources. More High-Fidelity Proofreading Polymerases from Genaxxon bioscience: - M3002 Pwo Proofreading Polymerase >- M3003 ReproFast Proofreading Polymerase >- M3004 Pfu Proofreading Polymerase >- M3012 ReproHot Proofreading Polymerase > With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.

AQ97 High Fidelity DNA Polymerase ist eine proofreading Polymerase mit hoher Amplifikationsgeschwindigkeit und sehr hoher Genauigkeit (>60-fach) gegenüber Taq DNA Polymerase. AQ97 ist ideal für schwierige Targets mit sehr niedrigem oder hohem GC-Gehalt geeignet. Neben diesen Eigenschaften zeichnet sich die AQ97 auch durch Ihre Eignung, lange Targets bis zu 18 kb, zu amplifizieren aus. Dies führt zu sehr genauen und zuverlässigen PCR-Ergebnissen.  AQ97 High Fidelity DNA Polymerase besitzt sowohl eine 5'→3' DNA Polymerase-Aktivität als auch eine 3'→5' proofreading Exonuklease-Aktivität, die es dieser Polymerase ermöglicht, Basenpaar-Fehlpaarungen zu korrigieren. AQ97 High Fidelity DNA Polymerase ist ein fusionierter Proteinkomplex aus DNA-Polymerase mit einer prozessivitätssteigernden DNA-Bindungsdomäne. Neben einer sehr schnellen und robusten Amplifikation komplexer und langer Zielmoleküle zeichnet sich die AQ97 High Fidelity DNA Polymerase durch eine hohe Genauigkeit aus, was dazu führt, dass auch für lange Amplicons eine genaue Amplifikation gewährleistet ist. Dadurch eignet sich die AQ97 besonders für PCR-Experimente, die eine Amplifikation mit sehr niedrigen Fehlerraten erfordern, wie z. B. Klonen/Subklonen, NGS-Anwendungen, SNP-Analysen und Mutagenese. Eigenschaften: • High Fidelity: >60x Taq fidelity• High elongation rate: 10 sec/kb (bis 6000bp pro Minute)• Long range amplification: 18 kb for human gDNA• 5'-3' polymerase acitivity and 3'-5' exonuclease activity.• generates blund ends.Picture 1: Comparison figures of fidelity values for AQ97 DNA Polymerase, AccuPol DNA Polymerase, two well-recognized high fidelity DNA polymerases P and Q and Taq DNA Polymerase were determined through NGS-based analysis of nucleotide misincorporation during PCR. Initially, PCR amplification was performed on a ~ 200 bp synthetic DNA target, generating PCR products for each of the tested polymerases (using recommended setup conditions).  Picture 2: Distribution of substitution errors. PCR was performed using Taq DNA Polymerase, AQ97 High Fidelity DNA Polymerase, high fidelity DNA Polymerase Q and high fidelity DNA Polymerase P. The PCR was followed by NGS sequencing of the PCR products. The number of substitutions at each PCR target position was calculated and plotted in diagram A. Substitutions include misincorporated nucleotides and deletions at each position. Non-polymerase errors are subtracted from the total number of errors to revel true polymerase errors. Non-polymerase errors include mutations caused by thermocycling-induced DNA damage, pre-NGS sample preparation and sequencing errors. In these diagrams the average number of substitutions for Taq DNA Polymerase (Taq average) and for AQ97 High Fidelity DNA Polymerase (AQ97 average) is also plotted. Diagram B magnifies the area near the detection limit, displaying more information about the number of substitutions for AQ97 High Fidelity DNA Polymerase, high fidelity DNA Polymerase Q and high fidelity DNA polymerase P. Click to enlarge diagrams.  

Especially suitable for use in microbiology, the DNA free Taq Polymerase DF Taq S for high specificity is virtually free of foreign DNA. The special purification procedure guarantees DF Taq Polymerase free of any DNA impurities, especially free of DNA from the conservative region of the 16S ribosomal gene. DNA free DF Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience DNA free Taq Polymerase allows amplification of DNA fragments >7 kb. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer S and separate MgCl2. The buffer is optimised for high specificity amplification of DNA-templates. Our complete buffer S contains 15mM MgCl2. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.

Especially suitable for use in microbiology, the DNA freeTaq Polymerase DF Taq E for high yields is virtually free of foreign DNA. The special purification procedure guarantees Taq Polymerase free of any DNA impurities, especially free of DNA from the conservative region of the 16S ribosomal gene. DNA free DF Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience Taq Polymerase allows amplification of DNA fragments >7 kb. Test sample available at a special price! No shipment costs within Germany. The test sample price will be refunded on the first official order of the product. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer E and separate MgCL2. The buffer is optimised for high yield amplification of DNA-templates. Our complete buffer E contains ammonium sulfate and 30mM MgCl2. The special purification procedure guarantees Taq-Polymerase free of any DNA impurities, especially free of DNA from the conservative region of the 16S ribosomal gene. The special purification procedure guarantees Taq-Polymerase free of any DNA impurities, especially free of DNA from the conservative region of the 16S ribosomal gene. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.

Ampliqon Taq DNA Polymerase is developed for automation and freeze drying. It is a glycerol free formulation of standard Ampliqon Taq DNA Polymerase and is well suited for automated routine PCR applications, or where accurate pipetting of small amounts is crucial. Features:- Glycerol free storage buffer- Suitable for automated high throughput testing- Suitable for robot-aided pipetting- Suitable for applications involving freeze drying- High product yield- Processes up to 5 kb- dUTP incorporation possible Description:Ampliqon Taq DNA Polymerase Glycerol Free exhibits both a 5'→3'dA DNA polymerase and a 5'→3' exonuclease activity. The 5'-3' exonuclease activity leaves a 3' overhang on the PCR product, which are convenient for direct T-A cloning. Taq DNA Polymerase Glycerol Free lacks 3'-5' exonuclease activity and proofreading ability. Why glycerol?Glycerol is normally a major part of the storage buffer for enzymes, and acts as a cryoprotectant. Glycerol disrupts the water structure and makes the buffer more cell like, thus stabilising the polymerase. Glycerol is a highly viscous liquid and is therefore difficult and time-consuming to pipet accurately, especially in smaller volumes. As a consequence, pipetting glycerol in fast robot-aided automation processes is almost an unsolvable challenge. Furthermore, the presence of glycerol in the enzyme buffer makes freeze drying impossible. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.  

Ampliqon Taq DNA Polymerase is ideal for routine PCR applications where high yield and reliable DNA amplification are required. DESCRIPTION Ampliqon Taq DNA Polymerase exhibits both a 5'→3' DNA polymerase and a 5'→3 exonuclease activity. Taq DNA Polymerase has no proofreading ability. The enzyme is shipped without buffer and MgCl2. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.

Ampliqon Taq DNA Polymerase is ideal for routine PCR applications where high yield and reliable DNA amplification are required. DESCRIPTION Ampliqon Taq DNA Polymerase exhibits both a 5'→3' DNA polymerase and a 5'→3 exonuclease activity. Taq DNA Polymerase has no proofreading ability. Enzyme shipped with standard buffer containing MgCl2. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.

Ampliqon Taq DNA Polymerase is ideal for routine PCR applications where high yield and reliable DNA amplification are required. DESCRIPTION Ampliqon Taq DNA Polymerase exhibits both a 5'→3' DNA polymerase and a 5'→3 exonuclease activity. Taq DNA Polymerase has no proofreading ability. The enzyme is shipped with buffer containing ammonium sulfate and MgCl2. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.

About TEMPase Hot Start DNA Polymerase TEMPase Hot Start DNA Polymerase has been designed to avoid spurious amplification and to diminish the formation of non specific priming events during your set up. TEMPase Hot Start DNA Polymerase allows for detection of low copy number targets and for multiplex PCR in a single reaction tube. TEMPase Hot Start DNA Polymerase is available together with different buffers. This one (A2251xx) is supplied with 10X ammonium sulfate buffer plus 10X combination buffer plus separate 25mM MgCl2. TEMPase Hot Start DNA Polymerase is also available together with 10X ammonium sulfate buffer and separate 25mM MgCl2 (A2211xx). Features     - Reaction set up at room temperature    - Increased sensitivity    - Increased specificity    - Increased product yieldDescription TEMPase Hot Start DNA Polymerase is a modified form of Ampliqon Taq DNA Polymerase, which is activated by heat treatment.A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during set up and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended.Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a heat activation step, releasing the active TEMPase Hot Start DNA Polymerase into the reaction. Please check also our SuperHot Hot Start Mastermix > with or without tracking dye. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.