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Overview:Proteinase K is a robust and versatile serine protease used extensively in molecular biology for the digestion of proteins in nucleic acid preparations. This solution, at a concentration of 20 mg/mL, is ideal for a wide range of applications including the preparation of DNA and RNA samples. The PCR grade quality ensures that the enzyme is free from contaminants that could interfere with downstream applications, making it highly suitable for sensitive techniques such as PCR.Product Details:ready-to-useConcentration: 20 mg/mLactive over a wide range of reaction conditionsAn elevation of the reaction temperature from 37°C to 50 - 60°C may increase the activity several timesQuality: PCR grade, ensuring high purity and no interference with PCR reactions.Applications: Suitable for DNA and RNA extractionRemoval of protein contaminantsPreparation of samples for various molecular biology techniques.Benefits:High Purity: The PCR grade quality guarantees minimal contamination, ensuring reliable results in sensitive applications. Versatility: Ideal for various molecular biology procedures, including sample preparation for PCR.Convenience: Ready-to-use solution at an optimal concentration for ease of use. Notes:The recommended working concentration of Proteinase K is 0.05 to 1 mg/mL. The activity of the enzyme is stimulated by 0.2 to 1% SDS and also by 1 to 4M ureaCa2+ protects Proteinase K against autolysis and increases the thermal stabilityStable over a wide pH range: 4.0 to 12.5, optimum pH 7.5 to 8.0Activity optimum: 50°C to 55°CRapid denaturation of enzyme occurs at temperatures above 65°CFeatures:No detected exonuclease, endonuclease or RNase activityConcentration ≥20 mg/mL / Activity ≥600 U/mLDNA content ≤200 pg/mLShipping conditions: Shipment on wet ice Storage buffer: 10 mM Tris/HCl, pH7.5; 1mM (CH3COO)2Ca; 50% glycerolProteinase K can be obtained from Genaxxon as Powder (M3036) > or as 20mg/mL Solution (M3037) >.Produced by Genaxxon bioscience GmbH, founded in 2002 by Dr. Norbert Tröndle to provide reliable products for PCR and custom cell culture media formulations, the Proteinase K Solution 20 mg/mL offers a dependable and efficient solution for your molecular biology needs. Achieve consistent, high-quality results with an enzyme designed specifically for your requirements.

Overview:Proteinase K is a robust and versatile serine protease used extensively in molecular biology for the digestion of proteins in nucleic acid preparations. This solution, at a concentration of 20 mg/mL, is ideal for a wide range of applications including the preparation of DNA and RNA samples. The PCR grade quality ensures that the enzyme is free from contaminants that could interfere with downstream applications, making it highly suitable for sensitive techniques such as PCR.Product Details:ready-to-useConcentration: 20 mg/mLactive over a wide range of reaction conditionsAn elevation of the reaction temperature from 37°C to 50 - 60°C may increase the activity several timesQuality: PCR grade, ensuring high purity and no interference with PCR reactions.Applications: Suitable for DNA and RNA extractionRemoval of protein contaminantsPreparation of samples for various molecular biology techniques.Benefits:High Purity: The PCR grade quality guarantees minimal contamination, ensuring reliable results in sensitive applications. Versatility: Ideal for various molecular biology procedures, including sample preparation for PCR.Convenience: Ready-to-use solution at an optimal concentration for ease of use. Notes:The recommended working concentration of Proteinase K is 0.05 to 1 mg/mL. The activity of the enzyme is stimulated by 0.2 to 1% SDS and also by 1 to 4M ureaCa2+ protects Proteinase K against autolysis and increases the thermal stabilityStable over a wide pH range: 4.0 to 12.5, optimum pH 7.5 to 8.0Activity optimum: 50°C to 55°CRapid denaturation of enzyme occurs at temperatures above 65°CFeatures:No detected exonuclease, endonuclease or RNase activitySpecific activity: >40 units/mg protein Activity: ≥30 units/mg lyophilizateSolubility: ≥20 mg/mLDNA content ≤10 pg/mgShipping conditions: Shipment on wet iceInhibitors of proteinase KWhich substances can inhibit proteinase K? Proteinase K can be inhibited by a variety of substances. These include ‘denaturing substances’, detergents and proteinase inhibitors. Sensitivity to inhibitors depends pretty much on the specific application (exact pH, concentration, temperature). Inhibitors frequently used in biochemistry and molecular biology are:SDS: High concentrations of SDS can denature and inactivate proteinase K.EDTA: EDTA is a chelating agent that can bind to metal ions that are essential for proteinase K activity.Urea: High concentrations of urea can denature and thereby inactivate proteinase K.Detergents: Some detergents, such as Triton X-100 or Tween® 20, can inhibit proteinase K, especially when the detergents are present in high concentrations.Protease inhibitors: Protease inhibitors, such as phenylmethylsulfonyl fluoride (PMSF), can irreversibly inhibit proteinase K.Chaotropic salts: Depending on the DNA extraction protocol, the presence of chaotropic salts or detergents may increase proteinase K activity. However, the same reagents may also inhibit proteinase K activity.Proteinase K can be obtained from Genaxxon as Powder (M3036) > or as 20mg/mL Solution (M3037) >.Produced by Genaxxon bioscience GmbH, founded in 2002 by Dr. Norbert Tröndle to provide reliable products for PCR and custom cell culture media formulations, the Proteinase K Solution 20 mg/mL offers a dependable and efficient solution for your molecular biology needs. Achieve consistent, high-quality results with an enzyme designed specifically for your requirements.

RedMasterMix (2x) - PCR MasterMix with red dye to help visualize pipetting and mixing steps. Electrophoresis can be performed immediately after PCR without the need of a gel loading buffer. This makes this MasterMix time efficient, cost efficient and a reliable choice for the best PCR results with high efficiency.  One tube, one pipetting step. RedMasterMix (2x) is delivered to you as a optimized, ready-to-use solution containing- Taq DNA Polymerase - dNTPs- MgCl2- a red dye- reaction bufferfor efficient amplification of DNA. Additionally the RedMasterMix (2x) contains an additive and a red dye for proceeding with electrophoresis after PCR without adding loading buffer. Just add your primers and template DNA. This PCR MasterMix (2x) has been optimized for use in routine PCR amplification of DNA templates in the range of 0.2-4kb. The special formulation of our PCR RedMasterMix (2x) can withstand repeated freezing/thawing without compromising yields, sensitivity or results, when used as directed. The PCR MasterMix with red dye is efficient (no left over of reagents), scalable from 10µL to 50µL and stable for at least 24 months.Our RedMastermix can also be used for Sanger sequencing. Just dilute the PCR reaction 1:8 after PCR, or purify using spin columns and then apply. Read now in our blog how Genaxxon's RedMasterMix can also simplify your laboratory work. Our Agaroses > and DNA Ladders > are ideally suited for subsequent electrophoresis of PCR products.

ProbeMastermix High ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains optimised amounts of all ingredients. Multiplex PCR: Applications at Genaxxon and at customers site have shown that the qPCR Probe Mastermix can be used for the simultaneous detection of up to four DNA targets in the same PCR reaction. For further details please refer to the product manual or contact us: info@genaxxon.com. Advantages of the Genaxxon ProbeMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Probe qPCR master mix high ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP 500nM ROX optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: Applied Biosystems® 5700, 7000, 7300, 7700, 7900, 7900 HT, Eppendorf Realplex4, StepOne™ and StepOnePlus™. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

Direct PCR from Plant Samples – Fast, Robust and Inhibitor-ResistantThe InhibiCore Rapid Plant DNA Q-Extraction PCR Kit is a streamlined, high-performance solution for rapid DNA analysis from plant material. It combines the efficient Plant DNA Q-Extraction Solution (M3230) with the highly robust InhibiCore Rapid Plant PCR MasterMix (5X) (M3250), enabling a complete workflow from sample preparation to reliable qPCR analysis.Plant tissues often contain strong PCR inhibitors such as polyphenols, polysaccharides, and other secondary metabolites. This kit is specifically designed to overcome these challenges, delivering reproducible and highly sensitive qPCR results even from difficult plant matrices.The Plant DNA Q-Extraction Solution enables fast and efficient DNA release without the need for conventional, time-consuming extraction procedures. Combined with the inhibitor-resistant PCR master mix, it creates a seamless Direct-PCR workflow that significantly reduces hands-on time and simplifies the overall process.The included InhibiCore Rapid Plant PCR MasterMix (5X) is based on a modified HotStart Taq DNA polymerase, ensuring high specificity, sensitivity, and robustness. Its exceptional inhibitor tolerance makes it ideally suited for demanding applications in plant research, agriculture, and quality control. The kit is also lyo-ready and includes dUTP for optional carry-over contamination control when used with UDG (Uracil-DNA Glycosylase). The HotStart polymerase enables convenient room-temperature setup without cooling or activation steps.Key BenefitsComplete solution for fast direct PCR from plant materialSeamless workflow: DNA extraction + qPCR in one systemHigh inhibitor tolerance for robust amplification from plant samplesFast and simple sample preparation without classical DNA isolationHigh sensitivity and specificity for reliable resultsTypical Applications Plant genotyping GMO and transgene detection Agricultural and plant research Plant pathogen detection Seed and quality control High-throughput direct PCR from plant material Applications examples:1. Sensitive qPCR analysis of chlorophyll gene targets from wheat lysatesReliable and sensitive detection of chlorophyll gene targets from plant lysates was demonstrated using qPCR with the InhibiCore Rapid Plant PCR MasterMix (5X) and fluorescent dye chemistry. A serial dilution of wheat lysate (10% to 0.001%), prepared with Plant DNA Q-Extraction Solution (M3230) was used to evaluate sensitivity and inhibitor tolerance.Successful amplification was achieved across all dilutions, confirming strong robustness against plant-derived inhibitors even at high lysate concentrations. A single, specific melt curve peak confirmed assay specificity with no non-specific amplification. Samples were prepared using the rapid 8-minute Plant DNA Q-Extraction Solution protocol, enabling fast DNA preparation without conventional purification steps. Figure 1: qPCR data by wheat lysate qPCR with InhibiCore Rapid Plant PCR MasterMix (5X).The figure shows qPCR amplification curves and melting curve analysis of a wheat lysate dilution series (10% to 0.001%) and a no-template control (NTC) using InhibiCore Rapid Plant PCR MasterMix (5X) and 1xGreenDye. Clear, concentration-dependent amplification is observed in the FAM channel, with reduced signal intensity at 10% lysate due to matrix effects. Melting curve analysis reveals a single specific peak for all positive samples, confirming specific target amplification without non-specific products. 2. Benchmark of Direct PCR performance from 10% tomato lysateThe performance of the InhibiCore Rapid Plant PCR MasterMix (5X) was evaluated against three competitor master mixes for qPCR from 10% tomato lysate. Samples were prepared using the Plant DNA Q-Extraction Solution and analyzed by fluorescent dye detection in the FAM channel.The InhibiCore Master Mix showed successful amplification with the earliest Ct values, demonstrating superior efficiency and the highest inhibitor tolerance compared to all tested competitors. Two competitor systems also produced amplification, while two showed strong inhibition with no detectable signals. Specific amplification was confirmed by a single, distinct melt curve peak with no evidence of non-specific products or primer-dimer formation.Figure 2: Plant direct PCR from plant samples Benchmark - inhibitor effect from Tomato lysateDirect PCR was performed using a cycling protocol of 95 °C for 3 min, followed by 40 cycles of 95 °C for 10 sec and 60 °C for 30 sec, with subsequent melt curve analysis. InhibiCore Rapid Plant PCR MasterMix (5X) and Competitor N showed successful amplification, while Competitor T and Competitor Q were strongly inhibited and did not generate reliable amplification curves. InhibiCore Rapid Plant PCR MasterMix (5X) exhibited the earliest Ct value among all tested systems, indicating the highest tolerance to plant-derived PCR inhibitors and superior performance in crude tomato lysates.

Why Choose Challenger Hotstart RT?Ultra-Fast cDNA synthesis – Achievable in just 2 minutes.Extended stability – In pre-assembled all-in-one reaction mixtures, Challenger Hotstart RT maintains full performance even after 7 days at 37 °C.Room-temperature setup – Pipette without cooling, saving time and reducing errors.Warm-start reverse transcriptase – Activated only at high temperatures, preventing nonspecific cDNA synthesis before the reaction begins.Exceptional thermostability – Over 99 % of enzyme activity remains sealed at 42 °C for controlled and reliable activation.Versatile applications – Ideal for rapid cDNA synthesis, RT-PCR, and RT-qPCR with challenging RNA templatesOptimized for High-Performance Reverse Transcription Workflows Challenger Hotstart RT is designed for demanding applications. Its Warm-Start technology keeps the enzyme inactive during setup, enabling precise control over the start of cDNA synthesis. The improved thermostability ensures robust performance: Challenger Hotstart RT remains inactive at 42 °C and is only activated at higher temperatures. Therefore, a standard reverse transcription reaction with oligo(dT) primers or random hexamer primers at 42 °C is not possible with this enzyme.Fast and Reliable cDNA SynthesisChallenger Hotstart RT allows efficient reverse transcription even with templates that have complex secondary structures or high GC content. Its enhanced thermostability ensures rapid and accurate cDNA generation without compromising specificity or yield.Long-Term Stability and Easy HandlingEngineered for routine and high-throughput lab work, Challenger Hotstart RT supports room-temperature setup, simplifying workflows and reducing errors. Its robust formulation guarantees reliable storage and shipping stability.Typical ApplicationscDNA synthesis from total RNA or mRNA, including difficult templates.One-step or two-step RT-qPCR with high sensitivity, even at low RNA input.Research and diagnostic workflows requiring reproducible results.High-throughput applications, including multiplexed panels or screening assays.Key Features SummaryUltra-Fast cDNA synthesis – as quick as 2 minutes.Extended stability – retains full performance after 7 days at 37 °C in premixed reaction setups.Warm-start reverse transcriptase – delayed activation improves specificity.High thermostability – supports challenging RNA templates. Room-temperature setup – simplifies workflow, reduces errors. Robust performance – suitable for routine and high-throughput applications.

Why Choose Challenger Hotstart RT-qPCR Mix?Room-temperature setup – Easy pipetting without cooling, saving time and reducing errors.warm-start reverse transcriptase – Prevents nonspecific amplification and allows primer/probe premixing.Superior contamination control – UDG/dUTP system eliminates carryover contamination for reliable qPCR results.Fast RT-qPCR protocols – Complete assays in just 30 minutes with a 5-minute reverse transcription step.High sensitivity RT-qPCR – Detect as few as 1–10 RNA copies.Flexible applications – Single and multiplex RT-qPCR for viral detection, gene expression, and diagnostics.Optimized for High-Performance qPCR WorkflowsThe 4× concentrated formulation allows high-throughput and multiplexing while keeping reaction efficiency high. The combination of warm-start RTase and antibody-modified Taq DNA polymerase and a RNAse inhibitor ensures precise control, so your reactions only start when you are ready.Contamination-Free One-Step RT-qPCRBuilt-in dUTP/UDG safeguards protect your assays from false positives due to carryover contamination. This, in combination with an RNase inhibitor is essential for labs running high-sensitivity viral RNA detection and diagnostic assay developments.Fast RT-qPCR Protocols Without Sacrificing AccuracyWith Challenger, you can speed up detection while keeping sensitivity intact:Standard program: 55 °C for 15 min RT, ~1 hr totalFast program: 55 °C for 5 min RT, ~30 min total Benchmark studies with HBV/HCV RNA samples confirmed superior stability and consistent Ct values compared to competitor brands.Long-Term Stability You Can TrustUnlike conventional RT-qPCR mixes, the Challenger Hotstart RT-qPCR Master Mix is engineered for long shelf life. Even under accelerated testing at 37 °C for 10 days, the mix maintained stable multiplex detection - ensuring reliable performance during storage and shipping.Example ApplicationsViral RNA detection: HBV, HCV, Influenza, RSV, SARS-CoV-2, hMPV, PIV1, and moreGene expression analysis: accurate detection of high- and low-copy RNA targetsClinical research & diagnostics: robust, reproducible dataHigh-throughput testing: multiplex RT-qPCR panels for respiratory, gastrointestinal, and hepatitis virusesMany more…Key Features SummaryOne-step RT-qPCR Master Mix with warm-start RTaseHigh sensitivity qPCR mix – detects single RNA copiesFast RT-qPCR protocol – 5 min RT, 30 min totalLong stability RT enzyme – stable at 37 °C for 10 daysUDG/dUTP contamination control – eliminates carryover risk4× concentrated format – supports multiplexing High sensitivity and stable detectionStable detection of single-digit copy templates using the Challenger Hotstart RT-qPCR 4X Mastermix:

Fast DNA Extraction & Hot-Start PCR KitThe DNA Q-Extraction Hot-Start PCR Kit combines our rapid DNA Q-Extraction Solution with the high-performance SuperHot Mastermix (2X) Blue. This all-in-one kit enables fast DNA extraction and reliable Hot-Start PCR – with PCR-ready DNA in just 8 minutes, without any additional purification steps. Ideal for genotyping and the amplification of DNA from a wide range of sources including mammalian tissues, bacterial cells, saliva, and oral swabs.Recent user data confirm that the DNA Q-Extraction Solution can also be successfully used with fungi samples, such as yeast and mold. The standard 8-minute protocol yields PCR-ready DNA without the need for additional clean-up or purification steps. This expands the range of applications and makes the DNA Q-Extraction Solution a versatile tool for rapid DNA extraction, even from more challenging sample types.Key FeaturesCombined DNA extraction & hot-start PCRFast 8-minute protocolNo centrifugation neededPCR-ready DNA from cells and tissuesIncludes loading dye for direct gel loading and pipetting controlApplicationThe simple one-step lysis is performed in two heating steps (65 °C / 98 °C) using a thermocycler or heating block. The resulting lysate contains PCR-ready DNA, suitable for standard and real-time PCR, without vortexing, or centrifugation.The included SuperHot Mastermix (2X) Blue contains a hot-start Taq polymerase for improved specificity and sensitivity. The integrated blue loading dye allows direct gel loading and visual control of pipetting.Kit ComponentsDNA Q-Extraction SolutionSuperHot Mastermix (2X) BlueStorageExtracted DNA is stable at -20 °C for up to one week, and for long-term storage at -80 °C.Also available as a non-hot-start version with our reliable Red Mastermix (2X) – perfect for robust results in routine PCR.Application Notes:application note genotyping with m3231_dna_q_extraction_solution_en_vs250527

Fast DNA Extraction & PCR in One KitThe DNA Q-Extraction PCR Kit Red combines the powerful DNA Q-Extraction Solution with our reliable Red Mastermix (2X) – enabling rapid and easy DNA extraction followed by PCR amplification in a single streamlined workflow. Perfect for genotyping, this kit delivers PCR-ready DNA in just 8 minutes from a variety of sample types such as mammalian cells, bacteria, saliva, buccal swabs, or tissue samples.Recent user data confirm that the DNA Q-Extraction Solution can also be successfully used with fungi samples, such as yeast and mold. The standard 8-minute protocol yields PCR-ready DNA without the need for additional clean-up or purification steps. This expands the range of applications and makes the DNA Q-Extraction Solution a versatile tool for rapid DNA extraction, even from more challenging sample types.Your Benefits at a GlanceFast 8-minute protocolNo vortexing, centrifugation or dilutionPCR-ready DNA directly from lysateSimple protocol: lysis and PCR with minimal handlingNon-toxic reagents – safe and convenientScalable and automation-friendlyDirect gel loading thanks to built-in loading dyeApplicationThe lysis is performed in two simple heating steps (65 °C for 6 min, 98 °C for 2 min) using a thermocycler or heating block. The resulting DNA is immediately ready for PCR without any further purification – ideal for genotyping applications. The included Red Mastermix (2x) ensures reliable amplification results and enables direct loading onto agarose gels with visual pipetting control.Kit ComponentsDNA Q-Extraction SolutionRed Mastermix (2X)Storage & StabilityExtracted DNA is stable at -20 °C for up to one week. For long-term storage, we recommend -80 °C.Also available as a hot-start version with our SuperHot Mastermix (2X) Blue – ideal for increased specificity with complex templates.Application Notes:application note genotyping with m3231_dna_q_extraction_solution_en_vs250527

The non-toxic Plant DNA Q-Extraction Solution provides fast and easy extraction of nucleic acids from various plant material (e.g. ivy or stinging nettle). The PCR-ready nucleic acid is extracted, using one tube and one reaction, in as little as 8 minutes. The one-step lysis is performed in either a thermocycler or heating block and is divided into two simple heating steps. The extracted DNA is then ready for PCR without further handling such as vortex, centrifugation or dilutions. The obtained DNA extract is stable at -20°C for up to one week and at -80°C for long term storage. For optimal genotyping results we recommend to use our Plant DNA Q-Extraction Solution together with our InhibiCore Rapid Plant PCR MasterMix (5X). We also offer them as a complete InhibiCore Rapid Plant DNA Q-Extraction PCR Kit for fast direct PCR from plant material. Features:- One-reagent set-up- Minimal handling- Rapid 8-minute protocol- PCR-ready DNA- no sample / yield loss- DNA extracts from plants- Non-toxic reagents- Scalable set-up- Automation-friendly Extraction ProtocolPreparation of DNA extraction should be performed in a separate area from that used for setting up the PCR reaction. 1. Thaw Plant DNA Q-Extraction Solution. For the first time use, mix the Plant DNA Q-Extraction solution well and aliquote it into smaller volumes. (Plant DNA Q-Extraction Solution has a cloudy appearance).2. Add your sample to a tube containing 100µL Plant DNA Q-Extraction Solution. Recommended sample sizes are shown in the table of the manual.3. Vortex the tube containing the sample and the DNA extraction solution for 15 sec. Make sure that the sample is completely covered by the Plant DNA Q-Extraction Solution.4. Transfer the tube to a heat block or a thermal cycler and incubate for 1. 65 °C for 6 min2. 98 °C for 2 min3. 4 °C (or cool down on ice) The DNA extract is now ready for PCR. Please note: Plant DNA lysate should be diluted 5- to 10-fold prior to PCR. We recommend performing a serial dilution. Applications examples:1. Sensitive qPCR analysis of chlorophyll gene targets from wheat lysatesReliable and sensitive detection of chlorophyll gene targets from plant lysates was demonstrated using qPCR with the InhibiCore Rapid Plant PCR MasterMix (5X) (M3250) and fluorescent dye chemistry. A serial dilution of wheat lysate (10% to 0.001%) was used to evaluate sensitivity and inhibitor tolerance.Successful amplification was achieved across all dilutions, confirming strong robustness against plant-derived inhibitors even at high lysate concentrations. A single, specific melt curve peak confirmed assay specificity with no non-specific amplification. Samples were prepared using the rapid 8-minute Plant DNA Q-Extraction Solution protocol, enabling fast DNA preparation without conventional purification steps. Figure 1: qPCR data by wheat lysate qPCR with InhibiCore Rapid Plant PCR MasterMix (5X).The figure shows qPCR amplification curves and melting curve analysis of a wheat lysate dilution series (10% to 0.001%) and a no-template control (NTC) using InhibiCore Rapid Plant PCR MasterMix (5X) and 1xGreenDye. Clear, concentration-dependent amplification is observed in the FAM channel, with reduced signal intensity at 10% lysate due to matrix effects. Melting curve analysis reveals a single specific peak for all positive samples, confirming specific target amplification without non-specific products. 2. Benchmark of Direct PCR performance from 10% tomato lysateThe performance of the InhibiCore Rapid Plant PCR MasterMix (5X) (M3250) was evaluated against three competitor master mixes for qPCR from 10% tomato lysate. Samples were prepared using the Plant DNA Q-Extraction Solution and analyzed by fluorescent dye detection in the FAM channel.The InhibiCore Master Mix showed successful amplification with the earliest Ct values, demonstrating superior efficiency and the highest inhibitor tolerance compared to all tested competitors. Two competitor systems also produced amplification, while two showed strong inhibition with no detectable signals. Specific amplification was confirmed by a single, distinct melt curve peak with no evidence of non-specific products or primer-dimer formation.Figure 2: Plant direct PCR from plant samples Benchmark - inhibitor effect from Tomato lysateDirect PCR was performed using a cycling protocol of 95 °C for 3 min, followed by 40 cycles of 95 °C for 10 sec and 60 °C for 30 sec, with subsequent melt curve analysis. InhibiCore Rapid Plant PCR MasterMix (5X) and Competitor N showed successful amplification, while Competitor T and Competitor Q were strongly inhibited and did not generate reliable amplification curves. InhibiCore Rapid Plant PCR MasterMix (5X) exhibited the earliest Ct value among all tested systems, indicating the highest tolerance to plant-derived PCR inhibitors and superior performance in crude tomato lysates. 3.  Easy sample handlingPlant DNA Q-Extraction Solution was used to extract genomic DNA from different plant tissues. For DNA extraction, 10mg tissue (leave) was added to 100µL of Plant DNA Q-Extraction Solution.Figure 3: DNA extracts of stinging needle and ivy. PCR tubes in duplicate with 100µL Plant DNA Q Extract Solution + 10mg leave material from either stinging neetles or ivy. A. stinging neetle and B. Ivy before extraction with Plant DNA Q Extraction Solution. C. stinging neetle and D. Ivy after extraction with Plant DNA Q Extraction Solution.Figure 4. The extracted DNA from ivy was amplified and two primer sets targeting: A. Genomic plant DNA (ITS 335 bp) or B. Chloroplast DNA (trnL 380 bp). The positive controls are 1 ng/reaction of DNA purified from stinging nettle using DNeasy Plant Mini Kit (Qiagen). Also included are duplicates of samples extracted in buffer without lysing agents, but using the Plant DNA Q-Extraction protocol (No treatment). All DNA extracts are amplified in duplicates.  More information? Watch our explainer video:

Highly pure, HPLC purified (>99%) dNTPs packaged as 4 separate 100mM solutions of dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. Genaxxon’s dNTP solutions have been optimized for use in DNA amplification and other related methods. Genaxxon dNTPs contain no measurable bacterial or human DNA. For long term storage and/or for repeated use, our recommendation is to aliquot the stock solutions. Solutions of dNTP sodium salts (dATP, dCTP, dGTP und dTTP) Concentration: 100 mM each nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mixes with 2 mM or 10 mM or the modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003) as well as the ready-to-use RedMastermix (M3029), already including dNTPs.

The non-toxic DNA Q-Extraction Solution provides fast and easy extraction of nucleic acids from various mammalian tissues (e.g. mouse tails, ear snips, liver, kidney, lung), saliva and bacteria. The PCR-ready nucleic acid is extracted, using one tube and one reaction, in as little as 8 minutes. The one-step lysis is performed in either a thermocycler or heating block and is divided into two simple heating steps. The extracted DNA is then ready for PCR without further handling such as vortex, centrifugation or dilutions. The obtained DNA extract is stable at -20°C for up to one week and at -80°C for long term storage.Recent user data confirm that the DNA Q-Extraction Solution can also be successfully used with fungi samples, such as yeast and mold. The standard 8-minute protocol yields PCR-ready DNA without the need for additional clean-up or purification steps. This expands the range of applications and makes the DNA Q-Extraction Solution a versatile tool for rapid DNA extraction, even from more challenging sample types. For optimal genotyping results we recommend to use our DNA Q-Extraction Solution together with our Red Mastermix Master Mix (2X) for PCR (M3029) or our SuperHot Mastermix Blue (2X) (M3007b). E.g. use 2µL to 5µL of the Q-Extract solution for a 25µL PCR reaction. For plant tissue we recommend using our specific Plant DNA Q-Extraction Solution (M3230). Features:- One-reagent set-up- Minimal handling- Rapid 8-minute protocol- PCR-ready DNA- no sample / yield loss- DNA extracts from mammalian tissues and bacteria- Non-toxic reagents- Scalable set-up- Automation-friendly Extraction ProtocolPreparation of DNA extraction should be performed in a separate area from that used for setting up the PCR reaction. 1. Thaw DNA Q-Extraction Solution. For the first time use, aliquot the DNA Q-Extraction solution into smaller volumes. (DNA Q-Extraction Solution has a cloudy appearance).2. Add your sample to a tube containing 100µL DNA Q-Extraction Solution. Recommended sample sizes are shown in Table 1 of the data sheet.3. Vortex the tube containing the sample and the DNA extraction solution for 15 sec. Make sure that the sample is completely covered by the DNA Q-Extraction Solution.4. Transfer the tube to a heat block or a thermal cycler and incubate for 1. 65 °C for 6 min2. 98 °C for 2 min3. 4 °C (or cool down on ice) The DNA extract is now ready for PCR. Anwendungsbeispiele:Efficient Amplification of DNA extracts from various mammalian tissue.DNA Q-Extraction Solution was used together with our RedMastermix (M3029 >) to extract and amplify genomic DNA from various mammalian tissues. For DNA extraction, 0.5-10 mg tissue or 20µL salvia was added to 100µL of DNA Q-Extraction Solution.M: DNA marker Iqon Low DNA Ladder. Lane 1-5: Different mouse tissues as depicted, GAPDH (266 bp). Lane 6: Chicken muscle tissue, HRPT1 (245 bp) and Lane 7: Human saliva, DMD17 (415 bp). DNA Q-Exctract Solution works over a wide range of sample sizesIn order to investigate the flexibility of DNA Q-Extraction Solution protocol, varying amounts of chicken muscle tissues were added to 100µL of DNA Q-Extraction Solution. The extraction was conducted as specified in the datasheet for DNA Q-Extraction Solution. The extracted DNA was subjected to PCR using the Genaxxon RedMastermix (M3029 >). The results show that the DNA Q-Extraction protocol provides the user with a high degree of flexibility over a wide range of applied sample amount.M: DNA marker Iqon Low DNA Ladder. Lanes 1-28: Varying amounts (mg) of chicken muscle tissue, HRPT1 (245 bp). Application Notes:application note genotyping with m3231_dna_q_extraction_solution_en_vs250527

GreenMasterMix without ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains optimised amounts of all ingredients. Advantages of the chemically modified SuperHot Taq polymerase used in the Genaxxon GreenMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Green qPCR master mix without ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP intercalating green fluorescent dye optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: BioRad CFX96 Touch™, CFX384 Touch™, CFX Connect™, DNA Engine Opticon® 2, Chromo4™, iCycler iQ™ and My iQ™ , Roche LightCycler® 480, LightCycler® 1536, LightCycler® Nano, LightCycler® 96 and QuantStudio™ instruments, Thermo Scientific™ PikoReal™, Cepheid SmartCycler®, Bio Molecular Systems Mic qPCR cycler, Qiagen Rotor Gene Q, Rotor Gene 6000, MyGo Mini and MyGo Pro. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

GreenMastermix High ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X PCR master mix is ready-to-use and contains optimised amounts of all ingredients. Advantages of the chemically modified SuperHot Taq polymerase used in the Genaxxon GreenMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Green qPCR master mix high ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP intercalating green fluorescent dye 500nM ROX optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: Applied Biosystems® 5700, 7000, 7300, 7700, 7900, 7900 HT, Eppendorf Realplex4, StepOne™ and StepOnePlus™. Other realtime PCR master mixes from Genaxxon are: M3023 - GreenMastermix No ROX >, M3045 - ProbeMastermix No ROX >, M3011 - GreenMastermix Low ROX >, M3031 - ProbeMastermix Low ROX >, M3052 - GreenMastermix High ROX >, M3010 - ProbeMastermix High ROX >.

ProbeMasterMix without ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains optimised amounts of all ingredients. Multiplex PCR: Applications at Genaxxon and at customers site have shown that the qPCR Probe Mastermix can be used for the simultaneous detection of up to four DNA targets in the same PCR reaction. For further details please refer to the product manual or contact us: info@genaxxon.com. Advantages of the chemically modified SuperHot Taq polymerase used in the Genaxxon ProbeMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Probe qPCR master mix without ROX contains all the necessary components in an optimized composition to carry out quantitative PCR. chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: BioRad CFX96 Touch™, CFX384 Touch™, CFX Connect™, DNA Engine Opticon® 2, Chromo4™, iCycler iQ™ and My iQ™ , Roche LightCycler® 480, LightCycler® 1536, LightCycler® Nano, LightCycler® 96 and QuantStudio™ instruments, Thermo Scientific™ PikoReal™, Cepheid SmartCycler®, Bio Molecular Systems Mic qPCR cycler, Qiagen Rotor Gene Q, Rotor Gene 6000, MyGo Mini and MyGo Pro. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

GreenMastermix Low ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains optimised amounts of all ingredients. Advantages of the chemically modified SuperHot Taq polymerase used in the Genaxxon GreenMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Green qPCR master mix high ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP intercalating green fluorescent dye 50nM ROX optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: Applied Biosystems® 7500, 7500 Fast and ViiA™ 7, QuantStudio™ instruments, Agilent Mx3000P™, Mx3005P™, Mx4000™ and AriaMx. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

ProbeMasterMix low ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X master mix is ready-to-use and contains optimised amounts of all ingredients. Multiplex PCR: Applications at Genaxxon and at customers site have shown that the qPCR Probe Mastermix can be used for the simultaneous detection of up to four DNA targets in the same PCR reaction. For further details please refer to the product manual or contact us: info@genaxxon.com. Advantages of the Genaxxon ProbeMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Probe qPCR master mix without ROX contains all the necessary components in an optimized composition to carry out quantitative PCR. chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP 50nM ROX optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: Applied Biosystems® 7500, 7500 Fast and ViiA™ 7, QuantStudio™ instruments, Agilent Mx3000P™, Mx3005P™, Mx4000™ and AriaMx. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

Agarose LE is a standard agarose for the separation of DNA in the size range between 100bp and 25kbp. It is suitable for all analytical and preparative electrophoresis of nucleic acids in routine gel electrophoresis. Depending on the concentration of Agarose LE used, the size range of nucleic acid separation will vary between 100bp and 25kbp. The low EEO makes this useful for a broad range of applications: PCR product analysis, restriction enzyme digest analysis, separation of RNA before blotting, etc. This agarose is comparable with, e.g. Agarose BioRagent, low EEO from Sigma or with the Universal-Agarose, peqGOLD from Peqlab. Free Taq DNA Polymerase test sample available! No shipping costs within Germany. Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available. Standard normal melting agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 100bp up to 25kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. They are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, agarase, as well as for "in-gel" DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.

Deoxynucleotide (dNTP) Solution Mix as an equimolar solution of ultrapure, HPLC purified (>99%) dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. The Genaxxon dNTP solutions are optimized for use in DNA amplification and other related methods. The Genaxxon dNTPs and dNTP-mixes contain no measurable bacterial or human DNA. For long term storage and/or for repeated use we do recommend to aliquot the stock solutions. Mixture of dNTP sodium salts (dATP, dCTP, dGTP und dTTP) Concentration: 10 mM each nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mix withv2 mM dNTP mix , our dNTP set, or modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003), as well as the ready-to-use RedMastermix (M3029), already including dNTPs.

Highly pure, HPLC purified dNTPs (>99%) delivered as a 2 mM solution of dATP, dCTP, dGTP and dTTP for use in qPCR, standard PCR, RT-PCR and Klenow reactions. Use 5 µL of Mix for PCR in 50µL reaction volume.The Genaxxon dNTP mix is optimized for its use in DNA polymerisation and related methods. Our dNTPs contain no measurable bacterial or human DNA. For storage for a prolonged periode of time we recommend to prepare small aliquots, especially in case of rare use. Mixture of dNTP sodium salt solutions (dATP, dCTP, dGTP und dTTP) Concentration: 2 mM of ach nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mix with 10 mM dNTP mix, our dNTP set , or modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003), as well as the ready-to-use RedMastermix (M3029), already including dNTPs.

The High-Fidelity Pfu proofreading DNA polymerase from Genaxxon bioscience is a thermostable, highly processive enzyme possessing 5'-3' DNA polymerase with additional 3'-5' proofreading exonuclease activity, which enables the correction of nucleotide incorporation errors. It has no 5'→3' exonuclease activity. The High-Fidelity Pfu DNA polymerase is a recombinant form of the hyperthermophilic archaebacteria Pyrococcus furiosus (Pfu). Pfu proofreading DNA polymerase shows an increased thermostability and a 10-times higher accuracy compared to Taq DNA polymerase. A mixture of Taq DNA Polymerase and Pfu DNA polymerase provides more robust synthesis of longer amplification products (Barnes, 1994. Proc. Natl. Acad. Sci. USA 91:2216-2220). Test sample available at a special price! The test sample price will be refunded on the first official order of the product. Features: 10-times higher accuracy compared to Taq DNA polymerase High-Fidelity polymerase Proofreading function (3' - 5' exonuclease activity) High thermo stability Generates blunt-end PCR products Generates PCR products for cloning and expression More High-Fidelity Proofreading Polymerases from Genaxxon bioscience:- M3003 ReproFast Proofreading Polymerase- M3002 Pwo Proofreading Polymerase- M3012 ReproHot (KOD) Proofreading Polymerase - AQ97 High Fidelity proofreading Polymerase With our high quality dNTPs as Set (M3015) or Mix (M3016) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.

The High-Fidelity Pwo proofreading DNA polymerase from Genaxxon bioscience is a thermostable, highly processive enzyme possessing 5'-3' DNA polymerase with additional 3'-5' proofreading exonuclease activity, which enables the correction of nucleotide incorporation errors. It has no 5'→3' exonuclease activity. The High-Fidelity Pwo DNA polymerase is a recombinant form of the hyperthermophilic archaebacteria Pyrococcus woesei. Pwo proofreading DNA polymerase shows an increased thermostability and a 10-times higher accuracy compared to Taq DNA polymerase. A mixture of Taq DNA Polymerase and Pwo DNA polymerase provides more robust synthesis of longer amplification products (Barnes, 1994. Proc. Natl. Acad. Sci. USA 91:2216-2220). Test sample available at a special price! The test sample price will be refunded on the first official order of the product. Features: 10-times higher accuracy compared to Taq DNA polymerase High-Fidelity polymerase Proofreading function (3' - 5' exonuclease activity) High thermo stability Generates blunt-end PCR products Generates PCR products for cloning and expression More High-Fidelity Proofreading Polymerases from Genaxxon bioscience:- M3003 ReproFast Proofreading Polymerase- M3004 Pfu Proofreading Polymerase- M3012 ReproHot (KOD) Proofreading Polymerase - AQ97 High Fidelity proofreading Polymerase With our high quality dNTPs as Set (M3015) or Mix (M3016) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.

High-quality Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience Taq Polymerase allows amplification of DNA fragment of up to >7 kb. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer and separate MgCL2.The enzyme is delivered with our buffer component 'Buffer-S'. The buffer is optimised for high specificity amplification of DNA-templates. Our complete buffer contains 15mM MgCl2. Free Taq DNA Polymerase test sample available!No shipping costs within Germany. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.

High-quality Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience Taq Polymerase allows amplification of DNA fragment of >7 kb. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer and separate MgCL2.The enzyme is delivered with our buffer component 'Buffer-E'. The buffer is optimised for high yield amplification of DNA-templates. Our complete buffer contains 25mM MgCl2. Taq DNA Polymerase test sample available! No shipping costs within Germany. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.

SuperHot Taq DNA Polymerase is a superior DNA Polymerase for Real Time PCR, Hot-Start PCR, low-copy number PCR, or PCR of difficult templates. The enzyme is developed to enhance the specificity, sensitivity and yield of DNA amplification. SuperHot Taq DNA polymerase is a chemicaly modified form of thermostable DNA polymerase Taq, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and before the first PCR cycle, the enzyme is not active and misprimed primers are not extended. As a result specifity and efficiency are increased by far compared to standard Taq DNA polymerase. Additionally, difficult targets with high GC-content can be amplified. For realtime PCR Genaxxon offers specially optimized 2-times qPCR mastermix >, respective a special Multiplex-PCR mastermix >. Features of Genaxxons SuperHot DNA Polymerase chemically modified increased specifity increased sensitivity for difficult templates with high GC-content Please have also a look on our SuperHot Taq 2-times Mastermix >. Even more convenient than the SuperHot Taq. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and favourable standard agarose (M3044) > we can offer additional products for your PCR.