Special Products

Why Choose Challenger Hotstart RT-qPCR Mix?Room-temperature setup – Easy pipetting without cooling, saving time and reducing errors.warm-start reverse transcriptase – Prevents nonspecific amplification and allows primer/probe premixing.Superior contamination control – UDG/dUTP system eliminates carryover contamination for reliable qPCR results.Fast RT-qPCR protocols – Complete assays in just 30 minutes with a 5-minute reverse transcription step.High sensitivity RT-qPCR – Detect as few as 1–10 RNA copies.Flexible applications – Single and multiplex RT-qPCR for viral detection, gene expression, and diagnostics.Optimized for High-Performance qPCR WorkflowsThe 4× concentrated formulation allows high-throughput and multiplexing while keeping reaction efficiency high. The combination of warm-start RTase and antibody-modified Taq DNA polymerase and a RNAse inhibitor ensures precise control, so your reactions only start when you are ready.Contamination-Free One-Step RT-qPCRBuilt-in dUTP/UDG safeguards protect your assays from false positives due to carryover contamination. This, in combination with an RNase inhibitor is essential for labs running high-sensitivity viral RNA detection and diagnostic assay developments.Fast RT-qPCR Protocols Without Sacrificing AccuracyWith Challenger, you can speed up detection while keeping sensitivity intact:Standard program: 55 °C for 15 min RT, ~1 hr totalFast program: 55 °C for 5 min RT, ~30 min total Benchmark studies with HBV/HCV RNA samples confirmed superior stability and consistent Ct values compared to competitor brands.Long-Term Stability You Can TrustUnlike conventional RT-qPCR mixes, the Challenger Hotstart RT-qPCR Master Mix is engineered for long shelf life. Even under accelerated testing at 37 °C for 10 days, the mix maintained stable multiplex detection - ensuring reliable performance during storage and shipping.Example ApplicationsViral RNA detection: HBV, HCV, Influenza, RSV, SARS-CoV-2, hMPV, PIV1, and moreGene expression analysis: accurate detection of high- and low-copy RNA targetsClinical research & diagnostics: robust, reproducible dataHigh-throughput testing: multiplex RT-qPCR panels for respiratory, gastrointestinal, and hepatitis virusesMany more…Key Features SummaryOne-step RT-qPCR Master Mix with warm-start RTaseHigh sensitivity qPCR mix – detects single RNA copiesFast RT-qPCR protocol – 5 min RT, 30 min totalLong stability RT enzyme – stable at 37 °C for 10 daysUDG/dUTP contamination control – eliminates carryover risk4× concentrated format – supports multiplexing High sensitivity and stable detectionStable detection of single-digit copy templates using the Challenger Hotstart RT-qPCR 4X Mastermix:

Why Choose Challenger Hotstart RT?Ultra-Fast cDNA synthesis – Achievable in just 2 minutes.Extended stability – In pre-assembled all-in-one reaction mixtures, Challenger Hotstart RT maintains full performance even after 7 days at 37 °C.Room-temperature setup – Pipette without cooling, saving time and reducing errors.Warm-start reverse transcriptase – Activated only at high temperatures, preventing nonspecific cDNA synthesis before the reaction begins.Exceptional thermostability – Over 99 % of enzyme activity remains sealed at 42 °C for controlled and reliable activation.Versatile applications – Ideal for rapid cDNA synthesis, RT-PCR, and RT-qPCR with challenging RNA templatesOptimized for High-Performance Reverse Transcription Workflows Challenger Hotstart RT is designed for demanding applications. Its warm-start technology keeps the enzyme inactive during setup, giving precise control over when cDNA synthesis begins. Enhanced thermostability ensures robust performance: activity remains fully sealed at 42 °C and is only released when the reaction starts.Fast and Reliable cDNA SynthesisChallenger Hotstart RT allows efficient reverse transcription even with templates that have complex secondary structures or high GC content. Its enhanced thermostability ensures rapid and accurate cDNA generation without compromising specificity or yield.Long-Term Stability and Easy HandlingEngineered for routine and high-throughput lab work, Challenger Hotstart RT supports room-temperature setup, simplifying workflows and reducing errors. Its robust formulation guarantees reliable storage and shipping stability.Typical ApplicationscDNA synthesis from total RNA or mRNA, including difficult templates.One-step or two-step RT-qPCR with high sensitivity, even at low RNA input.Research and diagnostic workflows requiring reproducible results.High-throughput applications, including multiplexed panels or screening assays.Key Features SummaryUltra-Fast cDNA synthesis – as quick as 2 minutes.Extended stability – retains full performance after 7 days at 37 °C in premixed reaction setups.Warm-start reverse transcriptase – delayed activation improves specificity.High thermostability – supports challenging RNA templates. Room-temperature setup – simplifies workflow, reduces errors. Robust performance – suitable for routine and high-throughput applications.