SimplyEnlight PCR - A single preparation step for PCR, gel electrophoresis and visualization!

SimplyEnlight PCR Starter-Kit – The All-in-One Solution for Efficient PCRFrom PCR to gel electrophoresis to visualization – all in just one preparation step!No additional pipetting of buffers or stains required. Save time and minimize contamination risks with the SimplyEnlight PCR Starter-Kit! Our innovative Red MasterMix Fluoro (2X) streamlines the PCR workflow by combining PCR amplification, gel loading dye, and nucleic acid staining in a single reaction. Additionally, the kit includes the pre-stained GenLadder 100 bp Plus, eliminating the need for extra staining.Your Key Benefits:Fast & efficient workflow – just one preparation step requiredNo additional gel staining – integrated fluorescent DNA stain for instant visualizationReduced contamination risk – fewer pipetting steps, improved reproducibilityNon-toxic fluorescent dye – environmentally friendly and safe disposalOptimized for screening & high-throughput applications – ideal for colony PCR and routine analysisContents of the SimplyEnlight PCR Starter-Kit: Red MasterMix Fluoro (2X) – the 3-in-1 solution: PCR MasterMix with DNA polymerase, buffer, dNTPs & MgCl₂ Integrated red loading dye for pipetting control Built-in fluorescent nucleic acid stain – no additional staining required GenLadder 100 bp Plus – pre-stained DNA marker:Instant DNA band visualization under UV or blue light No separate staining or pipetting required Loading Buffer I Fluoro (6X) – for additional DNA markers: Flexible use for staining extra DNA markers It doesn’t get any easier! With the SimplyEnlight PCR Starter-Kit, just mix your DNA and primers with the Red MasterMix Fluoro (2X) and start your PCR. After amplification, the PCR product can be analyzed and visualized via gel electrophoresis without any additional staining. Optimize your PCR workflow – with the SimplyEnlight PCR Starter-Kit from Genaxxon!

ready-to-use PCR Mastermix with red Loading Dye for visual control of the pipetting steps and additional fluorescent dye for fast and easy detection of the DNA bands. After PCR, the PCR mix can be pipetted directly into the gel pockets without adding loading buffer. This makes our RedMasterMix Fluoro (2x) even more time- and cost-saving than our proven Red Mastermix. The RedMasterMix Fluoro (2x) with fluorescent gel staining dye and red loading dye also features high specificity for best results. The RedMasterMix Fluoro (2x) is a ready-to-use mixture of: - Taq DNA Polymerase- PCR reaction buffer- dNTPs- MgCl2- red loading dye- fluorescence gel staining dye for DNA band detection in an optimal concentration for efficient amplification of DNA templates by PCR. Only the primers and the template DNA have to be added. At the same time, the PCR Matermix contains an additive and a red dye, which allows subsequent electrophoresis without the addition of loading buffer. After electrophoresis, detection is performed directly under blue light without further staining. This saves additional time and costs. The RedMasterMix Fluoro (2x) with fluorescent dye was developed for use in routine PCR up to 4 kb amplicon length. The special composition of the buffer guarantees reproducible results even after repeated thawing and freezing cycles. Our Red MasterMix (2x) Fluoro is shipped in convenient aliquots of 1.25mL. For more information on our SimplyEnlight PCR products, read our blog now. Genaxxon's SimplyEnlight PCR - Unlock the Power of Your PCR!

The GenLadder 100 bp Plus with gel staining dye and loading dye - ready-to-use is an innovation for high throughput approaches or colony screenings! Ideally suited to determine the size of double-stranded DNA between 100 and 1000 base pairs. The additional bands at 1.5kbp and 3.0kbp allow additional DNA sizes in this range to be assigned without having to use an additional 1kbp DNA marker. At the same time, the mixture also contains a fluorescent gel staining dye. Additional staining (pre- or post staining) or the addition of fluorescent dye prior to application is thus unnecessary and saves an additional pipetting step. At the same time, it is possible to check whether a PCR was successful or not before applying the PCR sample! This DNA ladder is an ideal match for our Red MasterMix Fluoro 2X, which also contains the gel staining dye. Together the ideal pair for Colony Screens! For more information on our SimplyEnlight PCR products, read our blog now. Genaxxon's SimplyEnlight PCR - Unlock the Power of Your PCR! The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp. The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 3000 bp (40ng/6µL), 2x 1500 bp (70ng/6µL), 1000 bp (50ng/6µL), 900 bp (40ng/6µL), 800 bp (40ng/6µL), 700 bp (30ng/6µL), 600 bp (30ng/6µL), 2x 500 bp (90ng/6µL), 400 bp (40ng/6µL), 300 bp (30ng/6µL), 200 bp (40ng/6µL), 100 bp (40ng/6µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 90 µg/mL. The recommended amount of DNA marker per lane is 0,5μg (6µL). GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3084 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

Loading buffer I Fluoro is a fluorescent reagent that produces instant visualization of DNA bands upon blue light or UV illumination of agarose gels. Supplied in 6X DNA Loading Buffer, Loading buffer I Fluoro is used to prepare DNA markers or samples for loading on agarose or polyacrylamide gels.  Loading buffer I Fluoro is the sensitive staining reagent available for detecting the double-stranded DNA (dsDNA). It contains three tracking dyes (bromophenol blue, xylene cyanol FF, and orange G) for visually tracking the DNA migration during the electrophoresis plus an additional fluorescence dye for detection of DNA bands without an additional staining of the gel. It is an ideal alternative to Ethidium Bromide (EtBr). Approximate fluorescence excitation / emission: 300, 495 / 537 nm, bound to nucleic acid. For more information on our SimplyEnlight PCR products, read our blog now. Genaxxon's SimplyEnlight PCR - Unlock the Power of Your PCR! Picture 1: Comparison of UV- and blue light excitation             Picture 2: Effect of EtBr plus UV on cloning efficiency.

pBLooK™ is a remarkable blue light LED transilluminator for nucleic acid detection with non-UV excitation conditions, hence, no damage to your DNA fragments. The excitation wavelength of the device is 470 nm, the best excitation condition suitable for most of all commercial fluorescent DNA staining dye. The specially designed device can inspect the amplification result in the tube after PCR reaction. Users can observe the signal simultaneously through the amber filter on the cover frame.  * Please note that BLooK™ is not suitable for ethidium bromide. Colours available: white, black, blue, red, pink, purple, yellow For more information on our SimplyEnlight PCR products, read our blog now. Genaxxon's SimplyEnlight PCR - Unlock the Power of Your PCR! Unit dimensions (W x L x H) 83 mm x 83 mm x 22 mm Weight (g) 100 g Input voltage AAA battery, 1.5 V x 3  pieces LED life (hours) < 30000 hours LED source Built-in blue light LED module Emission maxima (nm) 470 nm Store temperature 25 °C Operating temperature 40 °C Filter type Amber filter