SimplyEnlight PCR

SimplyEnlight PCR Starter-Kit – The All-in-One Solution for Efficient PCRFrom PCR to gel electrophoresis to visualization – all in just one preparation step!No additional pipetting of buffers or stains required. Save time and minimize contamination risks with the SimplyEnlight PCR Starter-Kit! Our innovative Red MasterMix Fluoro (2X) streamlines the PCR workflow by combining PCR amplification, gel loading dye, and nucleic acid staining in a single reaction. Additionally, the kit includes the pre-stained GenLadder 100 bp Plus, eliminating the need for extra staining.Your Key Benefits:Fast & efficient workflow – just one preparation step requiredNo additional gel staining – integrated fluorescent DNA stain for instant visualizationReduced contamination risk – fewer pipetting steps, improved reproducibilityNon-toxic fluorescent dye – environmentally friendly and safe disposalOptimized for screening & high-throughput applications – ideal for colony PCR and routine analysisContents of the SimplyEnlight PCR Starter-Kit: Red MasterMix Fluoro (2X) – the 3-in-1 solution: PCR MasterMix with DNA polymerase, buffer, dNTPs & MgCl₂ Integrated red loading dye for pipetting control Built-in fluorescent nucleic acid stain – no additional staining required GenLadder 100 bp Plus – pre-stained DNA marker:Instant DNA band visualization under UV or blue light No separate staining or pipetting required Loading Buffer I Fluoro (6X) – for additional DNA markers: Flexible use for staining extra DNA markers Please note: DNA Loading Buffer I Fluoro does show a small excitation peak in the UV range, but fluorescence is significantly stronger under blue light excitation (ca 490nm). We therefore recommend using blue light to achieve the best possible results.It doesn’t get any easier! With the SimplyEnlight PCR Starter-Kit, just mix your DNA and primers with the Red MasterMix Fluoro (2X) and start your PCR. After amplification, the PCR product can be analyzed and visualized via gel electrophoresis without any additional staining. Optimize your PCR workflow – with the SimplyEnlight PCR Starter-Kit from Genaxxon!

Ready-to-use PCR Mastermix with red Loading Dye for visual control of the pipetting steps and additional fluorescent dye for fast and easy detection of the DNA bands. After PCR, the PCR mix can be pipetted directly into the gel pockets without adding loading buffer. This makes our RedMasterMix Fluoro (2x) even more time- and cost-saving than our proven Red Mastermix. The RedMasterMix Fluoro (2x) with fluorescent gel staining dye and red loading dye also features high specificity for best results. The RedMasterMix Fluoro (2x) is a ready-to-use mixture of: - Taq DNA Polymerase- PCR reaction buffer- dNTPs- MgCl2- red loading dye- fluorescence gel staining dye for DNA band detection in an optimal concentration for efficient amplification of DNA templates by PCR. Only the primers and the template DNA have to be added. At the same time, the PCR Mastermix contains an additive and a red dye, which allows subsequent electrophoresis without the addition of loading buffer. After electrophoresis, detection is performed directly under blue light (ca. 490nm) without further staining. This saves additional time and costs. The RedMasterMix Fluoro (2x) with fluorescent dye was developed for use in routine PCR up to 4 kb amplicon length. The special composition of the buffer guarantees reproducible results even after repeated thawing and freezing cycles. Our Red MasterMix (2x) Fluoro is shipped in convenient aliquots of 1.25mL.Please note: The included fluorescent dye does show only a small excitation peak in the UV range, but fluorescence is significantly stronger under blue light excitation at 490nm. We therefore recommend using blue light to achieve the best possible results.

The GenLadder 100 bp Plus with gel staining dye and loading dye - ready-to-use is an innovation for high throughput approaches or colony screenings! Ideally suited to determine the size of double-stranded DNA between 100 and 1000 base pairs. The additional bands at 1.5kbp and 3.0kbp allow DNA sizes in this range to be assigned without having to use an additional 1kbp DNA marker. At the same time, the mixture also contains a fluorescent gel staining dye detectable with blue light (ca. 490nm excitation). Additional staining (pre- or post staining) or the addition of fluorescent dye prior to application is thus unnecessary and saves an additional pipetting step. This DNA ladder is an ideal match for our Red MasterMix Fluoro 2X, which also contains the gel staining dye. Together the ideal pair for Colony Screens! The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp. The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 3000 bp (40ng/6µL), 2x 1500 bp (70ng/6µL), 1000 bp (50ng/6µL), 900 bp (40ng/6µL), 800 bp (40ng/6µL), 700 bp (30ng/6µL), 600 bp (30ng/6µL), 2x 500 bp (90ng/6µL), 400 bp (40ng/6µL), 300 bp (30ng/6µL), 200 bp (40ng/6µL), 100 bp (40ng/6µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 90 µg/mL. The recommended amount of DNA marker per lane is 0,5μg (6µL).Please note: The included dye does show a small excitation peak in the UV range, but fluorescence is significantly stronger under blue light excitation. We therefore recommend using blue light to achieve the best possible results. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3084 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

The GenLadder 1kb bp Plus with gel staining dye and loading dye - ready-to-use is an innovation for high throughput approaches or colony screenings! Ideally suited to determine the size of double-stranded DNA between 250 and 10,000 base pairs. At the same time, the mixture also contains a fluorescent gel staining dye detectable with blue light (ca. 490nm excitation). Additional staining (pre- or post staining) or the addition of fluorescent dye prior to application is thus unnecessary and saves an additional pipetting step. This DNA ladder is an ideal match for our Red MasterMix Fluoro 2X, which also contains the gel staining dye. Together the ideal pair for Colony Screens! The Gene Ladder 1kbp Plus consists of 13 fragments in sizes between 250 - 10,000bp. The 1000bp and 3000bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 10.000 bp (28ng/6µL), 8000 bp (28ng/6µL), 6000 bp (28ng/6µL), 5000 bp (28ng/6µL), 4000 bp(18ng/6µL), 2x 3000 bp (92ng/6µL), 2500 bp (34ng/6µL), 2000 bp (34ng/6µL), 1500 bp (20ng/6µL), 2x 1000 bp (92ng/6µL), 750 bp (23ng/6µL), 500 bp (30ng/6µL), 250 bp (45ng/6µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 90 µg/mL. The recommended amount of DNA marker per lane is 0.5-0.6μg (5-6µL).Please note: The included dye does show a small excitation peak in the UV range, but fluorescence is significantly stronger under blue light excitation. We therefore recommend using blue light to achieve the best possible results. The GenLadder with gel staining dye is also available as a GenLadder 100bp Plus (M3237).GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3084 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

Loading buffer I Fluoro is a fluorescent reagent that produces instant visualization of DNA bands upon blue light or UV illumination of agarose gels. Supplied in 6X DNA Loading Buffer, Loading buffer I Fluoro is used to prepare DNA markers or samples for loading on agarose or polyacrylamide gels.  Loading buffer I Fluoro is the sensitive staining reagent available for detecting the double-stranded DNA (dsDNA). It contains three tracking dyes (bromophenol blue, xylene cyanol FF, and orange G) for visually tracking the DNA migration during the electrophoresis plus an additional fluorescence dye for detection of DNA bands without an additional staining of the gel.  Please note: DNA Loading Buffer I Fluoro does show a small excitation peak in the UV range, but fluorescence is significantly stronger under blue light excitation (ca. 490nm). We therefore recommend using blue light to achieve the best possible results.