- lyophilisierte qPCR-Mastermixe

LyoPlex Multiplex PCR master mix for fast and reliable quantification of up to 5 cDNA or gDNA targets in a single tube by multiplex, real-time PCR. This specially optimized and lyophilized master mix promotes fast multiplex real-time PCR. The combination of the specially designed hot start DNA polymerase together with a unique PCR buffer system as a lyophilized ready-to-use master mix ensures highly sensitive qPCR together with a very convenient storage at room temperature. Our new lyophilized Multiplex master mix for fast and easy multiplexing minimizes the need for optimization and makes the development of multiplex PCR assays fast and easy.- Amplification of multiple targets in a single tube (tested for up to 5 targets)- All-in-one master mix for convenient multiplexing- High specificity, sensitivity and product yield- Easy reaction setup at room temperature- stable for 24 months at room temperature The Multiplex PCR master mix is a premixed, ready-to-use solution containing our highly specific chemically modified Taq DNA Polymerase, highly pure dNTPs, MgCl2, additives and a reaction buffer at optimal concentrations which enable reliable multiplex PCR, e.g. microsatellite, clonality or gene rearrangement analysis. Just add your primers and template DNA. The Multiplex HS Master mix 2X is shipped in aliquots of 1.25mL. Our Standard Agarose LE > and especially our high resolution Agarose Tiny > are ideally suited for the subsequent electrophoresis analysis. Other realtime master mixes for your realtime PCR experiments can be found here >. Examples of Multiplex applications: F. Javier Pérez-Pérez and Nancy D. Hanson, Detection of Plasmid-Mediated AmpC β-Lactamase Genes in Clinical Isolates by Using Multiplex PCR, J. Clin. Microbiol. June 2002 vol. 40 no. 6 2153-2162. doi: 10.1128/JCM.40.6.2153-2162.2002. Tamara B. Souzaa, Diego M. Lozerb, Sônia M. S. Kitagawab, Liliana C. Spanob, Neusa P. Silvac and Isabel C. A. Scaletskya, Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli. J. Clin. Microbiol. March 2013 vol. 51 no. 3 1031-1033, doi: 10.1128/JCM.02478-12.

Lyophilized (freeze-dried) temperature stable and pre-mixed Hotstart master mix with all necessary components for probe realtime PCR (optimized buffer, dNTPs, Hotstart Taq) for difficult to amplify templates, qPCR and Multiplex applications. No cooling/cooling chain necessary! Our LyoMix and all ingredients are unopened stable at ambient temperature for at least 24 months! Our lyophilized qPCR master mix is also available as LyoBalls pre-portioned in PCR tube strips or PCR plates (M3069 > - 0.1mL tubes or M3070 > - 0.2mL tubes)! Our Hotstart Polymerase guarantees that during setup and the first PCR cycle the enzyme is not active and misprimed primers are not extended. As a result specificity and efficiency are increased by far compared to standard Taq DNA polymerase. Additionally, difficult targets with high GC-content can be amplified. The higher sensitivity improves multiplex PCR results.This lyophilized master mix in powder form is the perfect choice for a fast reaction setup that reduces the time required for pipetting and the possibility for pipetting errors. Just add PCR-grade water, template and primers to reconstitute the master mix and you are all set! Lyophilized realtime PCR master mix:• Hotstart Polymerase• increased specificity• increased sensitivity• for difficult to amplify templates with high GC content• for Multiplex applications• for qPCR• ready-to-useThis lyophilized Hotstart master mix is delivered in powder form that can be easily dispensed. With our high quality dNTPs as Set > (M3015.4100 and M3015.0250) or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.

While using our LyoBalls there is no need for cooling anymore! The balls and all ingredients are unopened stable at ambient temperature for at least 24 months! Lyophilized (freeze-dried) temperature stable, pre-mixed and pre-dispensed Hotstart master mix with all necessary components for probe realtime PCR (optimized buffer, dNTPs, Hotstart Taq) for difficult to amplify templates, qPCR and Multiplex applications. Only PCR-grade water has to be added to get the ready-to-use PCR master mix. The lyophilized master mix is supplied in a breakable 96-well PCR plate. The plate can be divided into individual "8's strips". Thus, you have the variability to run smaller or larger preparations, depending on your needs. No cooling/cooling chain necessary! The beads and all ingredients are unopened stable at ambient temperature for at least 24 months! Our Hotstart Polymerase guarantees that during setup and the first PCR cycle the enzyme is not active and misprimed primers are not extended. As a result specificity and efficiency are increased by far compared to standard Taq DNA polymerase. Additionally, difficult targets with high GC-content can be amplified. The higher sensitivity improves multiplex PCR results.This lyophilized master mix in a easy to handle bead format is the perfect choice for a fast reaction setup that reduces the time required for pipetting and the possibility for pipetting errors. Just add  PCR-grade water, template and primers to reconstitute the master mix and you are all set!Lyophilized realtime PCR master mix:• Hotstart Polymerase• increased specificity• increased sensitivity• for difficult to amplify templates with high GC content• for Multiplex applications•    for qPCR• ready-to-useThis lyophilized Hotstart master mix is delivered in single beads that can be easily dispensed. Each bead is equivalent to one 20µL PCR reaction. Our lyophilized qPCR Probe master mix is available in powder form (LyoMix, M3071) > and pre-dispensed in a 96-well PCR plate or strips. With our high quality dNTPs as Set > (M3015.4100 and M3015.0250) or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.

While using our LyoBalls there is no need for cooling anymore! The balls and all ingredients are unopened stable at ambient temperature for at least 24 months!  Lyophilized (freeze-dried) temperature stable, pre-mixed and pre-dispensed Hotstart master mix with all necessary components for probe realtime PCR (optimized buffer, dNTPs, Hotstart Taq) for difficult to amplify templates, qPCR and Multiplex applications. Only PCR-grade water has to be added to get the ready-to-use PCR master mix. The lyophilized master mix is supplied in a "break-a-way" 96-well PCR plate. The plate can be divided into individual "8's strips". Thus, you have the variability to run smaller or larger preparations, depending on your needs. No cooling/cooling chain necessary! The beads and all ingredients are unopened stable at ambient temperature for at least 24 months! Our Hotstart Polymerase guarantees that during setup and the first PCR cycle the enzyme is not active and misprimed primers are not extended. As a result specificity and efficiency are increased by far compared to standard Taq DNA polymerase. Additionally, difficult targets with high GC-content can be amplified. The higher sensitivity improves multiplex PCR results.This lyophilized master mix in a easy to handle bead format is the perfect choice for a fast reaction setup that reduces the time required for pipetting and the possibility for pipetting errors. Just add  PCR-grade water, template and primers to reconstitute the master mix and you are all set!Lyophilized realtime PCR master mix:•    Hotstart Polymerase•    increased specificity•    increased sensitivity•    for difficult to amplify templates with high GC content•    for Multiplex applications•    for qPCR•    ready-to-useThis lyophilized Hotstart master mix is delivered in single beads that can be easily dispensed. Each bead is equivalent to one 20µL PCR reaction. Our lyophilized qPCR Probe master mix is available in powder form (LyoMix, M3071) > and pre-dispensed in a breakable 96-well PCR plate. With our high quality dNTPs as Set > (M3015.4100 and M3015.0250) or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.