RNA Purification

Genaxxon´s Total RNA Purification Kit - Tissue is designed for the rapid and efficient purification of high quality RNA from 1-30mg of tissue (fresh or frozen) or up to 25mg Paraffin embedded tissue. The isolation protocol and buffer formulations were optimised for high isolation efficiency and purity of RNA. The Total RNA Purification Kit utilises spin columns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. The isolation procedure consists of 6 steps. In the first isolation step, the tissue is homogenized in order to disintegrate intercellular bonds (epithelial tissue) and fragmentize high-molecular proteins (muscle or connective tissue). Then the homogenate is lysed. Any RNases are inactivated. The three-step washing stage effectively removes impurities and enzyme inhibitors. The purified RNA is eluted using a low ionic strength buffer or RNase-free water and can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR and so forth. Genaxxon offers also ceramic beads > for rapid cell disintegration and other RNA purification kits >. With our HotScriptase RT Polymerase >, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step. Here you will find further Purification kits >.

Genaxxon´s Total RNA Purification Kit - Whole Blood/Cell culture is designed for the rapid and efficient purification of high quality RNA from whole blood (up to 300µL) or mammalian/bacterial/fungi cells.  The isolation protocol and buffer formulations were optimised for high isolation efficiency and purity of RNA. The Total RNA Purification Kit utilises spin columns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. The isolation procedure consists of 5 steps. In the first step, cells are harvested and then lysed in the second step. Any RNases are inactivated. The two-step washing stage effectively removes impurities and enzyme inhibitors. The purified RNA is eluted using a low ionic strength buffer or RNase-free water and can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR and so forth. Genaxxon offers also ceramic beads for rapid cell disintegration and other RNA purification kits. With our HotScriptase RT Mastermix, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step.

Ceramic beads for the lysis of tissue from a wide range of sample material, e.g. for RNA extraction. The kit contains 100 preps with 1.4mm ceramic beads (zirconia) and comes pre-proportioned to 1g in 2mL standard screw cap tubes. The beads are suitable for the homogenization of "soft" tissue from lung, liver, brain, kidney, spleen, skin, cells from cell culture, as well as for plant tissues such as e.g. Leaves or even whole insects. The usabl sample amount ranges from up to 200mg tissue, up to 200μL of cells suspended in water or isotonic saline solution, or up to 100mg wet weight of tissue culture cells grown in suspension (e.g., ≤10E7 mammalian cells). The beads can be used for a variety of samples and applications, e.g. B. for veterinary diagnostics (BSE screening), pharmacology and toxicology (drug detection), criminology and forensic medicine, food industry (GMO detection), microbiology in the environment (microorganism lysis). as well as the isolation of nucleic acids and proteins. They are chemically inert and do not bind nucleic acids. The beads are comparable with MP Biomedical No. 116913100 (equivalent to Lysing Matrix D) and are compatible with most commercially available homogenizers, like FastPrep® (MP Biomedicals), MagNA Lyser Instrument (Roche). You can reorder our RNA > and DNA purification columns > for the isolation of total RNA or DNA from enzymatic reactions or ceramic beads > separately. These articles can also be used with buffers/kits from other suppliers. With our HotScriptase RT PCR master mix >, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step. 

RNA Purification Mini Spin Columns for RNA purification kits of different suppliers. These RNA purification spin columns are a convenient and cheap alternative if buffers of already existing RNA purification kits are left but columns are out. For purification of total RNA. RNA can be used for: Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array analysis, next-generation sequencing. Elution volume of the RNA purification spin columns: 30-100µL, main sample types: tissue, cells, sample amount: 1 - 30mg. Additional Genaxxon offer also ceramic beads for rapid cell disintegration. You can reorder our RNA > and DNA purification columns > for the isolation of total RNA or DNA from enzymatic reactions or ceramic beads > separately. These articles can also be used with buffers/kits from other suppliers. With our HotScriptase RT Polymerase >, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step.

GENAzol is a ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA and proteins from biological samples. The single-phase solution of aqueous guanidine isothiocyanate solution can be used to isolate RNA, DNA and proteins in various fractions from cell and tissue samples, e.g. from humans, animals, plants, yeasts, bacteria or viruses (usually within one hour). Properties of GENAzol:- Parallel isolation of RNA, DNA and protein from the same sample- Superior suitability for lysis even with difficult samples- Optimized formulations and protocols for tissues, cells, blood, viruses and bacteria. Reliable purification of RNA from samples of different volumes and sourcesGENAzol is also suitable for small amounts of tissue (50–100mg) and cells (5× 10E6) as well as for larger amounts of cells (>10E7) and contains protocols for the purification of RNA from human, animal, plant or bacterial Rehearse. GENAzol maintains the integrity of the RNA due to the very effective inhibition of RNase activity when cells and components are dissolved during sample homogenization. GENAzol enables the simultaneous processing of a large number of samples. The entire isolation step is usually completed in 1 hour. The total RNA isolated with GENAzol is free from protein and DNA contamination. Procedure for the isolation of RNA, DNA and proteinsGENAzol enables sequential precipitation of RNA, DNA and proteins from a single sample. After homogenizing the sample with GENAzol, chloroform is added and the homogenate separates into a clear aqueous top layer (which contains RNA), a boundary layer and a organic bottom layer (with DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA precipitation from the organic boundary layer takes place with ethanol. Finally, the protein from the phenol-ethanol supernatant is precipitated with isopropanol. Precipitated RNA, DNA or protein is washed to remove impurities and then resuspended for use in subsequent applications.This is a dangerous good with UN number UN2821. Not for sale outside the European Union. UFI-Code: 7H2D-1Q71-Q00M-D0WU

Reliable RNA Stabilization for Optimal AnalysisThe Genaxxon Fix-it - Nucleic Acid Stabilization Reagent is a non-toxic reagent designed to stabilize and preserve RNA & DNA in biological samples such as tissues, cells, and blood. It rapidly penetrates the sample and effectively prevents DNA and RNA degradation by inhibiting Nuclease activity. This eliminates the need for immediate sample processing or storage in liquid nitrogen. Tissue samples can be collected and immersed in the Fix-it - Nucleic Acid Stabilization Reagent immediately after harvesting, ensuring high RNA quality and yield for subsequent isolation and analysis.AdvantagesReliable RNA stabilization – Protects RNA in tissues, cells, and blood by inhibiting RNases.Immediate protection – Quickly preserves RNA integrity after sample collection.Flexible storage – No need for immediate processing or freezing in liquid nitrogen.High-quality RNA – Ensures optimal yield for downstream applications.Safe and easy to use – Non-toxic, aqueous formulation for convenient handling.Highly effective - Stabilizes RNA for 1 day at 37°C, for 1 week at 25°C, for 1 month at +2°C to +8°C, or indefinitely at -20°C/-80°C.ApplicationsGene expression analysis – Provides high-quality RNA for RT-PCR and qPCR.RNA sequencing (RNA-Seq) – Preserves RNA integrity for transcriptome studies.Microarray analysis – Maintains RNA stability for accurate expression profiling.Biobanking – Enables long-term RNA storage without degradation.Field sample collection – Allows RNA preservation without immediate freezing.

G2 DNA/RNA Enhancer for improved DNA/RNA extraction!The G2 DNA/RNA Enhancer is developed to increase the yield of microbial DNA during DNA extraction from difficult matrices for example clay and soil samples. The primary function of G2 DNA/RNA Enhancer is to relieve inhibitory DNA - particle or other inhibitory DNA complexes. FEATURES- Inhibition of DNA and RNA sorption to clay particles- At least 2-10 fold increased yield of microbial DNA and RNA.- No trace of endonuclease-, nicking-, exonuclease- or RNase activity- Patent EP2 443 251 DESCRIPTIONG2 DNA/RNA Enhancer is available freeze dried with 0.1mm or 1.4mm ceramic beads in a 2mL tube. G2 DNA/Enhancer is also available in liquid format in three packing sizes including either 10, 50 or 100 reactions of 500µL. G2 DNA/RNA Enhancer should be used in combination with either a standardised extraction methods or commercial purification kits intended for DNA & RNA extraction. G2 DNA/RNA ENHANCER INCREASES DNA YIELD FROM SOIL SIGNIFICANTLY. G2 DNA/RNA Enhancer is available with 0.1mm ceramic beads (> A4201xx) or with 1.4mm ceramic beads (> A4214xx) and as solution (> A4200xx).Protocol for using G2 DNA/RNA Enhancer BeadsHow to use G2 DNA/RNA Enhancer in combination with commerical extraction kits:This protocol serves as a guideline for extraction of DNA and RNA from difficult matrices, such as soil clays, soils, activated coal etc. when using the G2 DNA/RNA Enhancer (G2) in combination with a commercial DNA or RNA extraction kit intended for soil.

G2 DNA/RNA Enhancer for improved DNA/RNA extraction!The G2 DNA/RNA Enhancer is developed to increase the yield of microbial DNA during DNA extraction from difficult matrices for example clay and soil samples. The primary function of G2 DNA/RNA Enhancer is to relieve inhibitory DNA - particle or other inhibitory DNA complexes. FEATURES- Inhibition of DNA and RNA sorption to clay particles- At least 2-10 fold increased yield of microbial DNA and RNA.- No trace of endonuclease-, nicking-, exonuclease- or RNase activity- Patent EP2 443 251 DESCRIPTIONG2 DNA/RNA Enhancer is available freeze dried with 0.1mm or 1.4mm ceramic beads in a 2mL tube. G2 DNA/Enhancer is also available in liquid format in three packing sizes including either 10, 50 or 100 reactions of 500µL. G2 DNA/RNA Enhancer should be used in combination with either a standardised extraction methods or commercial purification kits intended for DNA & RNA extraction. G2 DNA/RNA ENHANCER INCREASES DNA YIELD FROM SOIL SIGNIFICANTLY. G2 DNA/RNA Enhancer is available with 0.1mm ceramic beads (> A4201xx) or with 1.4mm ceramic beads (> A4214xx) and as solution (> A4200xx). Protocol for using G2 DNA/RNA Enhancer BeadsHow to use G2 DNA/RNA Enhancer in combination with commerical extraction kits:This protocol serves as a guideline for extraction of DNA and RNA from difficult matrices, such as soil clays, soils, activated coal etc. when using the G2 DNA/RNA Enhancer (G2) in combination with a commercial DNA or RNA extraction kit intended for soil.

G2 DNA/RNA Enhancer for improved DNA/RNA extraction!The G2 DNA/RNA Enhancer is developed to increase the yield of microbial DNA during DNA extraction from difficult matrices for example clay and soil samples. The primary function of G2 DNA/RNA Enhancer is to relieve inhibitory DNA - particle or other inhibitory DNA complexes. FEATURES- Inhibition of DNA and RNA sorption to clay particles- At least 2-10 fold increased yield of microbial DNA and RNA.- No trace of endonuclease-, nicking-, exonuclease- or RNase activity- Patent EP2 443 251 DESCRIPTIONG2 DNA/RNA Enhancer is available freeze dried with 0.1mm or 1.4mm ceramic beads in a 2mL tube. G2 DNA/Enhancer is also available in liquid format in three packing sizes including either 10, 50 or 100 reactions of 500µL. G2 DNA/RNA Enhancer should be used in combination with either a standardised extraction methods or commercial purification kits intended for DNA & RNA extraction. G2 DNA/RNA ENHANCER INCREASES DNA YIELD FROM SOIL SIGNIFICANTLY. G2 DNA/RNA Enhancer is available with 0.1mm ceramic beads (> A4201xx) or with 1.4mm ceramic beads (> A4214xx) and as solution (> A4200xx).