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- Molekularbiologie - weitere Produkte
Dextran sulfate 500 Sodium salt
€233.00*
Dextran sulfate is a polyanion produced by esterification of dextran with chlorosulphonic acid. Dextran sulfate 500 is a dextran with an average molecular weight of approx. 500,000 Daltons.
Anionic dextrans such as dextran sulfate (10% in hybridisation buffer) accelerate the hybridisation of nucleic acids by a factor of approx. 10.
Dextran sulfate is routinely used forSelective precipitation of lipoproteinsProbe hybridization of membrane-immobilized DNARelease of DNA from DNA-histone complexesInhibition of RNA binding to ribosomes
Salmon Sperm DNA (Na salt)
From
€92.44*
DNA extracted from salmon testes. To prevent unspecific binding of a DNA to the membrane in Southern- and Northern-Hybridisations salmon sperm DNA is added to the pre-hybridisation solution (Blotto, Denhardt's etc.). This Salmon Sperm DNA shows a molecular weight of ca. 10 - 2500 bp (20 - 650000 Da) with the main component of approx. 2000 bp.
The lyophilised DNA can be dissolved in sterile bidistilled water (stock solution e. g. 10mg/mL) and stored at -20°C. This DNA is used with a final concentration of 100μg/mL (Ref.: Sambrook, J., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, NY (1989), 9.48-9.49, 13.15. Silhavy, T., et al., Experiments with Gene Fusions, Cold Spring Harbor, NY (1984), 195.)