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Potassium tetrathionate for use in microbiology. This substance can be used as an incredient for Tetrathionate Broth Base acc. to Muller-Kauffmann (Dehydrated Culture Media) for microbiology.

Dextran sulfate is a polyanion produced by esterification of dextran with chlorosulphonic acid. Dextran sulfate 500 is a dextran with an average molecular weight of approx. 500,000 Daltons. Anionic dextrans such as dextran sulfate (10% in hybridisation buffer) accelerate the hybridisation of nucleic acids by a factor of approx. 10. Dextran sulfate is routinely used forSelective precipitation of lipoproteinsProbe hybridization of membrane-immobilized DNARelease of DNA from DNA-histone complexesInhibition of RNA binding to ribosomes

Reliable RNA Stabilization for Optimal AnalysisThe Genaxxon Fix-it - Nucleic Acid Stabilization Reagent is a non-toxic reagent designed to stabilize and preserve RNA & DNA in biological samples such as tissues, cells, and blood. It rapidly penetrates the sample and effectively prevents DNA and RNA degradation by inhibiting Nuclease activity. This eliminates the need for immediate sample processing or storage in liquid nitrogen. Tissue samples can be collected and immersed in the Fix-it - Nucleic Acid Stabilization Reagent immediately after harvesting, ensuring high RNA quality and yield for subsequent isolation and analysis.AdvantagesReliable RNA stabilization – Protects RNA in tissues, cells, and blood by inhibiting RNases.Immediate protection – Quickly preserves RNA integrity after sample collection.Flexible storage – No need for immediate processing or freezing in liquid nitrogen.High-quality RNA – Ensures optimal yield for downstream applications.Safe and easy to use – Non-toxic, aqueous formulation for convenient handling.ApplicationsGene expression analysis – Provides high-quality RNA for RT-PCR and qPCR.RNA sequencing (RNA-Seq) – Preserves RNA integrity for transcriptome studies.Microarray analysis – Maintains RNA stability for accurate expression profiling.Biobanking – Enables long-term RNA storage without degradation.Field sample collection – Allows RNA preservation without immediate freezing.

T4 DNA Ligase catalyzes the formation of a phosphodiester bonds between 5' phosphate and 3' hydroxyl termini in duplex DNA/RNA. The enzyme can join blunt end and cohesive end termini, repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.Cohesive End Ligation:For most cohesive end ligations, a 30 minute incubation at 20°C is sufficient. Incubations at 16°C for 4-16 hours are routinely used for the majority of applications. Ligation of blunt ends and single-base pair overhang fragments requires more enzyme to achieve the same extent of ligation as cohesive end DNA fragments. Ligation may be enhanced by addition of PEG, or by reducing the rATP concentration. T4 DNA Ligase requires ATP > as a cofactor. For PCR we recommend our cost-efficient Taq DNA Polymerase S (M3001 >), or our Pfu Proofreading Polymerase (M3004 >). Applications • Cloning of restriction enzymes generated DNA fragments • Cloning of PCR products • Connection of double-stranded oligonucleotide linkers or adapters with DNA • site-specific mutagenesis • Amplified fragment length polymorphism (AFLP) • Nicking repair in duplex DNA, RNA or DNA/RNA hybrids • Self-circulation of linear DNA.Definition of T4 Ligase activity2 different definitions of T4 Ligase activities are used!One Weiss unit is defined as the amount of enzyme required to convert 1 nmol of 32P-labeled inorganic pyrophosphate into Norit adsorbable material in 20 minutes at 37°C, using specified reaction conditions (Weiss, B., et al., (1968) J. Biol. Chem., 243, 4543). Norit is a type of activated carbon.A Cohesive End Unit is defined as the amount of enzyme required to give 50% ligation of Hind III fragments of lambda DNA (5´ DNA termini concentration of 0.12µM (300µg/mL)) in 20µL of 1X T4 DNA Ligase Buffer in 30 minutes at 16°C. The conversion factor between the two activities is: One Cohesive End Ligation units (CEL units) corresponds to 0.015 Weiss units. One Weiss unit therefore corresponds to 66.67 Cohesive End Ligation units (CEL units).